Labshake search
Citations for Macherey-Nagel GmbH :
151 - 200 of 2219 citations for rno mir 134 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... NucleoSpin Gel and PCR Clean-up kit was purchased from Macherey-Nagel. SF Cell Line 4D-Nucleofector X Kit S were purchased from Lonza ...
-
bioRxiv - Genetics 2021Quote: cleaning step using the Nucleospin Gel and PCR Cleanup kit (Macherey-Nagel). Samples were PCR amplified using the Phusion High-Fidelity DNA Polymerase (NEB ...
-
bioRxiv - Genetics 2021Quote: ... purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Libraries from the primary screen were sequenced 2×50bp on the HiSeq 2500 Platform (18 pM loading concentration ...
-
bioRxiv - Molecular Biology 2021Quote: ... were purified with NucleoSpin Gel and PCR Clean-Up kit (Macherey-Nagel), and quantified with the Quant-It PicoGreen double stranded DNA Assay kit (Invitrogen) ...
-
bioRxiv - Genomics 2021Quote: ... purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel), digested with HindIII-HF (NEB ...
-
bioRxiv - Genomics 2021Quote: ... The DNA was purified using the PCR Clean-up kit (Macherey-Nagel).
-
bioRxiv - Microbiology 2022Quote: ... followed by the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel), and sent to Eurofins (Ebersberg ...
-
bioRxiv - Molecular Biology 2022Quote: ... NucleoSpin Gel and PCR Clean-up kit was purchased from Macherey-Nagel. 2 mm electroporation cuvettes were acquired from Cell Projects Ltd ...
-
bioRxiv - Molecular Biology 2023Quote: ... using the NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... purified using the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) and ligated using the NEBuilder HiFi DNA assembly kit (BioLabs).
-
bioRxiv - Genomics 2023Quote: ... purified with a PCR clean-up and gel extraction kit (Macherey-Nagel) and quantified with the Kapa Library Quantification Kit (Kapa Biosystems) ...
-
bioRxiv - Genetics 2024Quote: ... Resulting PCR products were purified with the Bioanalysis NucleoSEQ Kit (Macherey-Nagel), and 15 µL of each eluate was mixed with the same volume of Hi-Di formamide (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2024Quote: ... gel-purified with the NucleoSpin Gel and PCR Cleanl1Jup kit (MACHEREY-NAGEL) and assembled using the In-Fusion HD Cloning Kit (Takara Bio) ...
-
bioRxiv - Microbiology 2023Quote: ... and purified with the NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel). The DNA binding reactions were performed in interaction buffer (10 mM Tris-HCl [pH 7.5] ...
-
bioRxiv - Genomics 2021Quote: Total RNA was extracted from each time point samples using the NucleoSpin RNA kit (Macherey-Nagel) as described in our earlier publications (Oláh et al ...
-
bioRxiv - Genetics 2020Quote: ... PCR reactions were separated on agarose gels and PCR fragments isolated using a Nucleo-Spin Gel and PCR Clean-up kit (Macherey-Nagel). Sanger sequencing of PMEL and KIT ...
-
bioRxiv - Cell Biology 2021Quote: ... and a ∼500 bp portion around the predicted CRISPR cut site was amplified by PCR and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). This fragment was then ligated into an EGFP-N vector using Xho1 and Apa1 restriction sites and transformed into DH5-a competent cells ...
-
bioRxiv - Biochemistry 2022Quote: ... pre-tRNAPheGAA2-2 and human pre-tRNATyrgta8-1 and tRNAArgtct3-2 were amplified by PCR of respective pUC19 vectors and template DNA was isolated using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). RNA substrates were produced by run-off in vitro transcription using T7 RNA polymerase (New England Biolabs ...
-
bioRxiv - Molecular Biology 2022Quote: ... at least eight separate tubes were used for PCR and then pooled and purified by using PCR and gel extraction kit (Macherey-Nagel) according to manufacturer’s protocol ...
-
bioRxiv - Systems Biology 2023Quote: ... The PCR products of correct size were purified by agarose gel electrophoresis (NucleoSpin Gel and PCR Clean-up Kit, Macherey-Nagel). The purified linear DNA was used for cloning of pTS040 by Gibson assembly (NEBuilder HiFi DNA Assembly Reaction ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... PCR products were confirmed on 2% agarose gels and purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Purified PCR products were quantified using the Quant-iT dsDNA kit (Invitrogen ...
-
bioRxiv - Biochemistry 2022Quote: ... PCR products were visualized on a 1% TAE agarose gel and purified using the Nucleospin PCR Clean-up Kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Three independent PCR reactions from the same library were combined after PCR and purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Sequencing was carried out on an Illumina HiSeq XTen at Macrogen ...
-
bioRxiv - Molecular Biology 2024Quote: ... All PCR or vector digestion products were purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel; #740609.50). The final plasmids were transformed into competent E.coli XL1-Blue ...
-
bioRxiv - Microbiology 2019Quote: ... purification of the high molecular weight product by gel extraction kit using PCR Clean-up kit (Macherey-Nagel), and subsequent ligation using T4 DNA ligase.
-
bioRxiv - Microbiology 2019Quote: ... were pooled and purified with Qiagen PCR CleanUp kit (Macherey-Nagel, Hoerdt, France). Paired-end sequencing (2×300 bp ...
-
bioRxiv - Molecular Biology 2019Quote: ... purified using the NucleoSpin® Gel and PCR Clean-Up kit (Macherey-Nagel) according to the manufacturers’ instructions ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... excised and purified with a PCR clean-up gel extraction kit (Macherey-Nagel), then cloned into the pJET 1.2/blunt vector (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... samples were cleaned up (NucleoSpin gel and PCR clean up kit, Macherey-Nagel), and size groups per sample were pooled according to specimen numbers per group ...
-
bioRxiv - Microbiology 2021Quote: ... Amplified DNA was purified using Gel and PCR Clean-up kits (Macherey-Nagel) and single-end Sanger-sequenced with the corresponding forward primer at Eurofins Scientific ...
-
bioRxiv - Genetics 2020Quote: ... sliced and purified with Nucleospin PCR and Gel Clean-up Kit (Macherey-Nagel). Concentration was measured in a NanoDrop device.
-
bioRxiv - Cell Biology 2022Quote: ... and purified using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). In vitro transcription reaction was carried out in a final volume of 100 μl transcription mix containing 80 mM HEPES (pH 7.5) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Reactions were purified using the NucleoSpin PCR and Gel Purification Kit (Macherey-Nagel). Samples were pooled after qPCR quantification ...
-
bioRxiv - Cell Biology 2021Quote: ... The DNA template was purified with NucleoSpin PCR Clean-up kit (Macherey-Nagel). Pre-miR-181a-1 in vitro synthesis was performed using T7 MEGAshortscriptTM kit (Ambion ...
-
bioRxiv - Microbiology 2022Quote: ... and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). The plasmid N and inserts were digested and purified ...
-
bioRxiv - Microbiology 2022Quote: ... and then purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel). Amplicons were then assembled (1:1 insert:vector molar ratio ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Nucleospin® extract II kit (Macherey-Nagel) according to manufacturer’s instructions and were Sanger sequenced (Eurofins UK).
-
bioRxiv - Developmental Biology 2023Quote: ... DNA was purified with the NucleoSpin Gel and PCR cleanup Kit (Macherey-Nagel) and used for quantitative PCR to detect gene-specific regions near the respective transcriptional start (Suppl ...
-
bioRxiv - Molecular Biology 2023Quote: ... and then purified using NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL) with Buffer NTB.
-
bioRxiv - Genetics 2023Quote: ... DNA was purified using NucleoSpin PCR clean-up kit (Macherey-Nagel, cat# 740609), dissolved with ddH2O and adjusted to a concentration of 0.6 μM ...
-
bioRxiv - Immunology 2024Quote: ... followed by purification using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Linearized DNA templates (1 µg ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Nucleospin® extract II kit (Macherey-Nagel) according to manufacturer’s instructions and were Sanger sequenced (Eurofins UK).
-
bioRxiv - Cancer Biology 2020Quote: ... All amplified PCR products were isolated using silica membrane spin column technique (NucleoSpin® Gel, PCR clean up kit Macherey-Nagel, Germany) and were eluted in 20 µl of nuclease free water ...
-
bioRxiv - Plant Biology 2021Quote: ... Labelling was performed by PCR using vector-specific DY-682-labelled primers followed by PCR purification with NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL). Gel shifts were visualized using a LiCor Odyssey imaging system at 700 nm.
-
bioRxiv - Microbiology 2020Quote: ... Polymerase chain reaction (PCR) products and plasmid digestion derivatives were purified using NucleoSpin® Gel and PCR Clean-up kits (Macherey-Nagel). Plasmids were extracted from E ...
-
bioRxiv - Microbiology 2019Quote: ... All single fragment PCR reactions were realized using Phusion High-Fidelity DNA Polymerase (ThermoScientific) and PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). Overlap PCRs were carried out using 100 ng of each purified PCR products and the resulting fragment of interest was purified from agarose gel ...
-
bioRxiv - Immunology 2021Quote: ... The amplified PCR product is then purified from gel using the “Nucleospin Gel and PCR Clean-up” kit (Macherey-Nagel, Reference: 740609.250).
-
bioRxiv - Microbiology 2021Quote: ... PCR reactions were realized using Phusion High-Fidelity DNA Polymerase (ThermoScientific) and PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). The overlap PCR product was purified from agarose gel and was directly used to transform wild type F ...
-
bioRxiv - Plant Biology 2023Quote: ... The two resulting PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Ref. REF 740609.50; Macherey-Nagel, Düren, Germany), digested at 37°C overnight using FastDigest Eco31I (Thermo Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Both gel purifications and PCR clean-ups were performed using the NucleoSpin® PCR and Gel Clean Up Kit (Macherey-Nagel, Germany). DNA concentrations and purities were measured on a NanoDropTM 2000 (Thermo Fisher Scientific Inc. ...