Labshake search
Citations for Macherey-Nagel GmbH :
201 - 250 of 2220 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... PCR reactions were realized using Phusion High-Fidelity DNA Polymerase (ThermoScientific) and PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). The overlap PCR product was purified from agarose gel and was directly used to transform wild type F ...
-
bioRxiv - Plant Biology 2023Quote: ... The two resulting PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Ref. REF 740609.50; Macherey-Nagel, Düren, Germany), digested at 37°C overnight using FastDigest Eco31I (Thermo Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Both gel purifications and PCR clean-ups were performed using the NucleoSpin® PCR and Gel Clean Up Kit (Macherey-Nagel, Germany). DNA concentrations and purities were measured on a NanoDropTM 2000 (Thermo Fisher Scientific Inc. ...
-
bioRxiv - Microbiology 2023Quote: ... The dephosphorylated pEX18Tc vector and PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). TEDA reaction was then carried out by mixing 150 ng of pEX18Tc with the corresponding PCR products at a molar ratio of 1:4 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The NaOH was neutralized by the addition of 1 M HCl and the cDNA was purified by a PCR-clean-up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Germany) according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2019Quote: ... genomic fragments containing the gRNA target site were amplified with PCR and purified using NucleoSpin Gel and a PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Then 200 ng of each of the PCR products derived from the transfected and non-transfected cells were denatured at 95° C for 5 min and reannealed ...
-
bioRxiv - Plant Biology 2021Quote: ... The amplicons were purified from the PCR reagent tube via the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).
-
bioRxiv - Genomics 2021Quote: ... Gel extraction is performed to isolate the PCR product with the correct size range using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). PacBio sequencing library preparation for both libraries was done by the Genomics Core Leuven (KU Leuven) ...
-
bioRxiv - Microbiology 2023Quote: ... Read length was assessed by DNA agarose gel electrophoresis (1% w/v) and amplicons were purified from the PCR mix using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, DE) following manufacturer’s instructions.
-
bioRxiv - Synthetic Biology 2023Quote: ... PCR amplicons were purified via gel purification or column purification using the NucleoSpin Gel and PCR Clean–up kit (Macherey-Nagel, Cat.#740609.50). Amplicon sequences were verified by Sanger sequencing using the Overnight Mix2Seq Kit service (Eurofins Genomics) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... we purified the amplification reaction following the PCR clean-up protocol of the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Finally ...
-
bioRxiv - Bioengineering 2024Quote: ... PCR products from genomic DNA and cDNA were purified using NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Allentown, PA, USA), and quality was validated via agarose gel electrophoresis.
-
bioRxiv - Biophysics 2024Quote: ... NucleoSpin® plasmid isolation kit and NucleoSpin® gel and PCR clean-up kit were purchased from Macherey-Nagel (Germany). PierceTM protease inhibitor tablets EDTA free and Pierce™ high capacity Ni-IMAC resin were purchased from Thermo Scientific (international) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The products were then purified using the NucleoSpin PCR Clean-up kit (Macherey-Nagel). We then performed 2×250 bp paired-end MiSeq sequencing (Illumina).
-
bioRxiv - Bioengineering 2022Quote: ... Plasmid extraction and polymerase chain reaction (PCR) purification kits were ordered from Macherey-Nagel GmbH & Co ...
-
bioRxiv - Microbiology 2020Quote: ... Purified rbcL amplicons (NucleoSpin® gel and PCR clean-up kit, Macherey-Nagel, France) were either Sanger-sequenced directly or cloned into pGEM-T easy vector plasmids using competent Escherichia coli JM109 cells (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... gel-purified using the Nucleospin® Gel and PCR Clean-Up kit (Macherey-Nagel), and cloned into the LentiORF pLEX-MCS Vector (Thermo Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: ... Gel extractions were performed using NucleoSpin Gel and PCR Clean-up Kits (Macherey-Nagel). Vectors were assembled via AQUA cloning technology (Beyer et al. ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using the Nucleospin Gel and PCR Clean up kit (Macherey-Nagel) by following the manufacturer’s instructions with slight modifications ...
-
bioRxiv - Neuroscience 2020Quote: ... RNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Germ line transmission was identified by mating sexually mature adult fish to mitfaw2/w2 fish and ...
-
bioRxiv - Neuroscience 2021Quote: ... were purified using a Nucleospin Gel and PCR Clean-up kit (#740609, Macherey-Nagel) and subjected to DNA sequencing to verify if off-target activity had occurred.
-
bioRxiv - Plant Biology 2021Quote: ... DNA was purified with the NucleoSpin PCR Cleanup kit (#740609; Macherey-Nagel, Düren, Germany) with buffer NTB (#740595.150 ...
-
bioRxiv - Synthetic Biology 2019Quote: ... digested with KpnI and purified again with PCR Clean-up kit (Macherey-Nagel, Germany). The p416-loxP-KmR-TEFmut6-yECitrine plasmid was linearized with KpnI and dephosphorylated with Antarctic Phosphatase (New England Biolabs ...
-
bioRxiv - Physiology 2019Quote: ... purified with the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Italy) and subsequently sequenced.
-
bioRxiv - Microbiology 2020Quote: ... The resulting PCR product was purified by column capture (Macherey-Nagel Nucleospin mini kit) and sequenced (Eurofins Scientific ...
-
bioRxiv - Pathology 2021Quote: Amplicons were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and submitted to cycle-sequencing reaction by BigDye Terminator v.3.1 Cycle Sequencing kit (Ther-mo Fisher) ...
-
bioRxiv - Plant Biology 2019Quote: ... cleaned using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany) and sent for Sanger sequencing (Source Biosciences ...
-
bioRxiv - Microbiology 2019Quote: ... One sample was processed with the Nucleospin PCR Clean-up kit (Macherey-Nagel, Germany) according to the manufacturer’s instructions and the resulting DNA was used for qPCR ...
-
bioRxiv - Biochemistry 2022Quote: Plasmids and polymerase chain reaction (PCR) products were purified using kits from Macherey-Nagel. PCR fragments were amplified using Q5 High-Fidelity 2x Master Mix (New England Biolabs) ...
-
bioRxiv - Biochemistry 2022Quote: ... The amplicons were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) following the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2022Quote: ... each pool purified (Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit) and send to GENEWIZ Germany GmbH for Illumina sequencing (Amplicon-EZ Sequencing Service ...
-
bioRxiv - Genetics 2023Quote: ... then column purified (Nucleospin® gel and PCR clean-up kit, Macherey-Nagel, France) in EB elution buffer (Qiagen) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Synthons were purified with the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) following the manufacturer’s protocol using a vacuum manifold ...
-
A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... The reaction was purified using a NucleoSpin PCR Clean-up Kit (Macherey-Nagel 740609.250) and eluted in 30 µL 1X TE buffer.
-
bioRxiv - Biophysics 2022Quote: ... and the 3’-terminal poly A) were amplified by PCR and purified using the NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2021Quote: ... Amplified libraries were purified using a NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel) and size selected by electrophoresis on non-denaturing 6% TBE-Urea gels ...
-
bioRxiv - Developmental Biology 2021Quote: ... the DNA was purified by column purification (Gel and PCR clean up kit, Macherey-Nagel) and used for RNA synthesis (Megashortscript T7 Kit ...
-
bioRxiv - Neuroscience 2021Quote: RNA was purified using the Nucleospin gel and PCR clean-up kit from Macherey-Nagel. Complementary DNA (cDNA ...
-
DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsbioRxiv - Synthetic Biology 2019Quote: ... which was cleaned using a NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel #NC0389463). 10 ng per individual sample from the pooled clean product was sent to Quintara Biosciences or FornaxBio and ran on an Illumina MiSeq.
-
bioRxiv - Plant Biology 2021Quote: ... the bands were purified using the NucleoSpin Gel and PCR Clean kit (Macherey-Nagel®) and sequenced.
-
bioRxiv - Plant Biology 2020Quote: ... PCR products of Acc1 were column-purified with the NucleoSpin Extract ΙΙ Kit (Macherey-Nagel).
-
bioRxiv - Genetics 2021Quote: ... PCR of genomic DNA extracted 3 days after nucleofection using NucleoSpin Tissue kit (Macherey-Nagel) was performed using specific primers and Sanger sequenced (Stabvida ...
-
bioRxiv - Microbiology 2021Quote: ... reactions were cleaned up with the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel), and ligated to pGEM-T Easy (Promega ...
-
bioRxiv - Microbiology 2021Quote: ... DNA fragments were purified by using NucleoSpin Gel and PCR Clean-up kits (Macherey-Nagel). Oligos were synthesized by TAG Copenhagen A/S and DNA sequences were sequenced at Eurofins Genomics ...
-
bioRxiv - Molecular Biology 2022Quote: ... Immunoprecipitated DNAs were purified using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel).
-
bioRxiv - Plant Biology 2021Quote: ... isolated on agarose gel and purified (Macherey-Nagel NucleoSpin Gel and PCR Clean-up Kit). Transformants were generated using electroporation as previously described 61 ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... probes were purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Duren, Germany), re-suspended in 50 µL of DEPC water ...
-
bioRxiv - Synthetic Biology 2020Quote: ... and a 400bp band was extracted using the NucleoSpin PCR Cleanup Kit (Macherey-Nagel, 740609.250). The concentration of DNA in the resulting eluent was determined via a Qubit 2 fluorometer (Thermofisher) ...
-
bioRxiv - Immunology 2020Quote: ... DNA was purified by using the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL). The transposed DNA was amplified by PCR for 10-12 cycles by using the Nextera DNA Library Preparation Kit and Nextera XT Indexing Kit (Illumina) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Amplified fragments were purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany) and DNA concentrations were measured by NanoDrop.