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Citations for Macherey-Nagel GmbH :
1 - 50 of 61 citations for Streptavidin magnetic beads since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... NucleoMag™ magnetic separation beads (Macherey-Nagel, Düren, Germany) were used for double-ended size selection targeting an insert size of 300 bp ...
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bioRxiv - Genomics 2023Quote: ... and purified using NucleoMag™ NGS magnetic beads (Macherey-Nagel, Düren, Germany) prior to DNA libraries synthesis.
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bioRxiv - Genomics 2023Quote: ... Amplification products were cleaned up using NGS clean-up magnetic beads (Macherey-Nagel), introducing a size selection through a concentration of 1.2x beads per sample and eluted in a total amount of 25 µL EBT buffer (1 mM EDTA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... For the final purification with magnetic beads (NucleoMag NGS kit, Macherey-Nagel, Düren, Germany), we used a ratio of 0.65:1 bead solution to the library to discard small fragments originating from heteroduplexes that had passed the size selection ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Size selection was performed using NucleoMag™ magnetic separation beads (Macherey-Nagel, Düren, Germany) targeting an insert size of 300 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were purified using magnetic beads (NucleoMag NGS Clean-up and Size Select, Macherey-Nagel) according to the manufacturer’s instructions in a DNA to beads volume ratio of 1:1.8 ...
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bioRxiv - Cell Biology 2023Quote: All samples were pooled and cleaned using a 1.2× NucleoMag NGS clean-up and size select magnetic beads (Macherey-Nagel – 7449970.5) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: The final PCR amplified library was submitted to two consecutive 1× NucleoMag NGS Clean-up and Size select magnetic bead selections (Macherey-Nagel – 7449970.5) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The 3C template was purified using Nucleomag PCR beads (“P-beads”, Macherey-Nagel #744100.1), eluted in 450 μl 5mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl of a 40% slurry Ni-NTA agarose beads (20 μl beads, Macherey-Nagel) were added followed by an incubation at 4°C for 45 min with shaking ...
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bioRxiv - Biochemistry 2024Quote: ... soluble proteins were purified by affinity chromatography on corresponding beads: Ni-NTA agarose beads (Macherey-Nagel) or glutathione agarose 4B beads (Macherey-Nagel) ...
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bioRxiv - Systems Biology 2024Quote: Fecal pellets (∼40 mg) were weighed in sterile ceramic bead tubes (NucleoSpin Bead Tubes; Macherey-Nagel, Dueren, Germany) and extracted with 1 mL of chilled methanol (−20 °C ...
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bioRxiv - Microbiology 2020Quote: ... Equilibrated Protino Ni-IDA beads (Macherey-Nagel) were added to the supernatant and incubated for 1 h at 4 °C in an over-head rotor ...
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bioRxiv - Microbiology 2023Quote: ... Equilibrated Protino Ni-IDA beads (Macherey-Nagel) were added and rotated for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... or glutathione agarose 4B beads (Macherey-Nagel). PBS supplemented with 200 mM imidazole or 10 mM reduced glutathione was used as the elution buffer for proteins with His6 or GST tags ...
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bioRxiv - Microbiology 2021Quote: ... containing 80 μl Ni-NTA agarose beads (Macherey-Nagel) during 1 h beforehand washed in water and pre-equilibrated in equilibrium buffer 20 mM Tris– HCl pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Ni-IDA affinity beads (Macherey-Nagel, Bethlehem, PA, USA) were used to purify TBC-DEG assemblies ...
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bioRxiv - Cell Biology 2022Quote: ... Precleared lysates were incubated with Ni-NTA beads (Macherey-Nagel) for 1 h at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Cleared extract was batch incubated with Ni-NTA beads (Macherey-Nagel) for 1 hour on a rotating wheel at room temperature for His-tag affinity purification ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
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bioRxiv - Evolutionary Biology 2024Quote: ... crushed the samples using five MN Beads Type D (Macherey-Nagel). We crushed leg samples with only three beads ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was loaded onto 1 ml Ni-NTA agarose beads (Macherey-Nagel), pre-equilibrated with 10 ml wash buffer and rotated end-over-end for 1h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA eluted from beads was purified using Nucleospin RNA XS (Macherey-Nagel) and processed for cDNA synthesis using a High Capacity RNA to cDNA kit (Applied Biosystems).
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The circadian clock gene circuit controls protein and phosphoprotein rhythms in Arabidopsis thalianabioRxiv - Systems Biology 2021Quote: ... with 167µl GSH-agarose bead suspension (Protino glutathione agarose 4B, Macherey-Nagel), a binding incubation of 30min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Filter pieces were transferred into MN Bead Tubes Type A (Macherey-Nagel) containing 800 μl of buffer RLT Plus that were pre-chilled at −80°C for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... Supernatant was incubated with Protino Glutathione Agarose 4B beads (Macherey-Nagel, 745500) in a rotatory shaker overnight at +4°C ...
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bioRxiv - Genetics 2023Quote: ... the DNA was purified with NucleoMag P-beads (Macherey-Nagel, Düren, Germany). A second round of digestion was performed with 50 U of DpnII or Csp6I RE (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Double-stranded DNA was purified using NucleoMag beads (Macherey-Nagel Inc., Bethlehem, PA) and subsequently subjected to random amplification with primer FR20RV prior to sequencing library preparation [20].
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bioRxiv - Immunology 2021Quote: ... Barcoded amplicons were pooled and purified using Nucleo Mag NGS beads (Macherey-Nagel), then sequenced using HiSeq 2500 (Illumina) ...
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bioRxiv - Immunology 2024Quote: ... organs were homogenized at 4 °C using Bead Tubes type F (Macherey-Nagel) and the lysate was further used for RNA isolation ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was transferred to 400 μL Ni-NTA agarose beads (Macherey-Nagel), which had previously been washed once with ddH2O and twice with lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... were prepared with 20 µL of NucleoMag B-Beads (NucleoMag Pathogen kit, Macherey-Nagel) and 975 µL of Buffer NPB2 (NucleoMag Pathogen kit ...
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bioRxiv - Biophysics 2022Quote: ... NiNTA beads were given into a empty Protino column (Macherey-Nagel, Düren, Ger, 745400.10) and washed again ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl of a 40% slurry Ni-NTA agarose (20 μl beads, Macherey-Nagel) were added followed by an incubation for 45 min at 4°C with shaking ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with preequilibrated Protino Ni-NTA beads (1 ml resin, Macherey-Nagel) at 4°C for 1h ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed mechanically via bead-beating and DNA was purified on columns (Macherey-Nagel, Germany). A robotized platform (Biomek4000 ...
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bioRxiv - Developmental Biology 2023Quote: ... elegans animals were transferred to 2 ml MN Bead Tubes Type A (Macherey-Nagel, Düren, Germany) and lysed using a Precellys Bead Beating system with an additional Cryolys cooling module (Bertin Instruments ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged proteins in soluble extracts were purified by GSH-agarose beads (Macherey-Nagel, Düren, Germany) from which were eluted by the addition of 50 mM reduced L-glutathione (dissolved in 25 mM Tris-HCl pH 8).
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bioRxiv - Biochemistry 2023Quote: ... the supernatant discarded and the beads transferred onto a Protino 14 ml gravity column (Macherey-Nagel). After 20 ml of additional equilibration using buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplified DNA was then purified using NucleoMag NGS Clean-up and Size Select beads (Macherey-Nagel) using a 0.5x ratio of beads to DNA to initially clear large DNA fragments from the library and then a 1.5x ratio of beads to supernatant to adsorb DNA fragments of appropriate size for sequencing ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then crushed the remaining tissues using five MN Beads Type D (Macherey-Nagel, Duren, Germany). We extracted total genomic DNA from each sample using NucleoMag® Tissue Kit (Macherey-Nagel ...
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... SUV420H1 proteins were isolated by incubating the soluble fraction for 2 hrs with GST beads (Macherey-Nagel). The beads were washed with two column volumes of wash buffer (20 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... The filters were then individually placed into 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel), added with 1,200 µL MWA1 lysis buffer and stored at - 80°C until nucleic acids extraction as specified in the corresponding section below.
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bioRxiv - Neuroscience 2022Quote: ... beads were magnetized and 150 µl RA1 lysis buffer from NucleoSpin RNA Plus XS Kit (40990.50, Macherey-Nagel) was added to the beads ...
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bioRxiv - Molecular Biology 2024Quote: ... The TEV eluate was incubated with 200 µl pre-washed Protino® Ni-NTA Agarose beads (Macherey-Nagel) for 1 h at 4°C in a rotating wheel ...
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bioRxiv - Microbiology 2022Quote: ... faeces and buccal swabs were homogenized in a bead mill (Precellys) in lysis buffer (Macherey-Nagel; cat no. 740984.10). After homogenization ...
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bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were supplemented with 20 mM imidazole and incubated with Protino Ni-NTA agarose beads (Macherey-Nagel) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... slight adaptations were made from manufacturer’s instructions: half MCE filters were placed into individual 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel) and added with 1,200 µL MWA1 lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: Pull-down experiments using GST (Glutathion-S-Transferase)-fused proteins were performed using glutathione agarose beads (Macherey-Nagel, Düren, Germany). The beads were incubated with the GST-fused proteins for 1 h ...