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Citations for Macherey-Nagel GmbH :
201 - 250 of 319 citations for Solid Phase Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... The band size between 137nt–149nt was selected and purified using Gel Extraction Kit (Macherey-Nagel, Germany). The quality of the purified product (sRNA-seq libraries ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was isolated from each sample using the NucleoSpin Plant RNA extraction kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions and in combination with Fruit-mate for RNA Purification solution (Takara Bio Europe SAS ...
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bioRxiv - Biochemistry 2019Quote: ... followed by gel extraction and clean-up using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). The products were mixed at an equimolar ratio and sent for paired-end sequening on Illumina HiSeq2000 to Novogene Bioinformatics Technology Co. ...
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bioRxiv - Microbiology 2021Quote: ... total cellular RNA was isolated using the NucleoSpin RNA extraction kit (Macherey-Nagel/Takara, San Jose, California). cDNA was generated from bulk RNA with the high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: Total RNAs were extracted from each pool using the Nucleospin RNA II extraction kit (Macherey-Nagel, Germany). Pools were ground in 350 µL Lysis Buffer and 3.5 µL β-mercaptoethanol using the homogenizer Precellys®24 Dual (Bertin ...
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bioRxiv - Genomics 2021Quote: ... BACs were extracted from bacterial culture using the NucleoBond® Xtra Midi plasmid extraction kit (Macherey-Nagel) according to the manufacturer’s provided instructions ...
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bioRxiv - Plant Biology 2021Quote: ... ∼500 mg of rhizosphere soil was used for the NucleoSpin Soil gDNA Extraction Kit (Macherey-Nagel, Germany), and ∼130 mg of roots for the DNeasy Plant DNA Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... Total RNAs from harvested cells were extracted using the NucleoSpin RNA extraction kit (Cat. # 740955.250, MACHEREY-NAGEL), and 0.4-1 μg RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Cat ...
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bioRxiv - Genomics 2020Quote: ... We extracted genomic DNA using NucleoSpin Microbial DNA extraction kit according to the manufacturer’s instructions (Macherey-Nagel). Genomic DNA for strain Y55 was extracted independently using the QIAGEN Blood & Culture DNA Midi Kit ...
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bioRxiv - Immunology 2022Quote: ... lymph nodes and bone marrow cells by manual extraction with the NucleoSpin® Tissue kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... ~500 mg of rhizosphere soil was used for the NucleoSpin Soil gDNA Extraction Kit (Macherey-Nagel, Germany), and ~130 mg of roots for the DNeasy Plant DNA Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used for cloning reactions were purified by using the Nucleospin gel extraction kit (Macherey-Nagel) according to the manufacturer’s specifications ...
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bioRxiv - Plant Biology 2023Quote: RNA samples were extracted using the Nucleospin plant RNA extraction kit (Macherey-Nagel GmbH & Co. KG, Germany). cDNAs were synthesized using PrimeScript RT Mastermix (Takara Bio Inc. ...
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bioRxiv - Immunology 2023Quote: ... RNA from tumours was extracted and purified from tumours using the NucleoSpin RNA extraction kit (Macherey-Nagel). Library preparation and sequencing were performed by Genewiz (Leipzig ...
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bioRxiv - Microbiology 2024Quote: Total genomic DNA was extracted using the NucleoMag® Tissue extraction kit (Macherey-Nagel™, Düren, Germany) and the V3-V4 region of the 16S rDNA gene was amplified with the prokaryotic primers 343F (5′-ACGGRAGGCAGCAG – 3′ ...
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bioRxiv - Biochemistry 2021Quote: ... Both peptide segments were purified by C18 reverse phase (RP)-HPLC on a Nucleosil C18 column (300 Å, 250 × 10 mm) (Macherey-Nagel, Düren, Germany), maintained at 25°C ...
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bioRxiv - Cell Biology 2020Quote: ... were purified using an endonuclease free plasmid extraction kit (NucleoBond Xtra Maxi Plus EF, Macherey-Nagel, cat# 740426.10).
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bioRxiv - Immunology 2021Quote: ... RNA extraction (two replicates per cell line) was performed using the Nucleospin® RNA Plus Kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from N/Tert or HFK cells using the NucleoSpin RNA extraction kit (Macherey-Nagel). RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: DNA and RNA extractions were performed using the RNA x DNA from soil kit (740143, Macherey-Nagel, Germany). The RNA sample was treated with Turbo DNA-free Kit (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... purification of the high molecular weight product by gel extraction kit using PCR Clean-up kit (Macherey-Nagel), and subsequent ligation using T4 DNA ligase.
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bioRxiv - Plant Biology 2019Quote: ... Frozen samples were subjected to RNA extraction using the NucleoSpin RNA Plant kit (Cat# 740949, Macherey-Nagel, Germany) following the manufacturer’s protocol.
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bioRxiv - Genetics 2021Quote: ... Nucleic acid extraction was performed with the NucleoSpin DNA Insect Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: RNA extraction of in vitro cultured Th17 cells was performed with the NucleoSpin RNA XS Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were purified via gel extraction using a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). Illumina sequencing adapters were added with Phusion High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA extraction was performed using a combination protocol between the NucleoSpin® Plant II kit from Macherey-Nagel and the DNeasy plant mini kit from QIAGEN ...
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bioRxiv - Plant Biology 2024Quote: ... and subjected to genomic DNA extraction using the Nucleo Mag Plant kit (Macherey-Nagel GmbH &Co. KG, Germany) in the automated nucleic acid extractor Maelstrom 4800 (TANBead Taiwan Advanced Nanotech Inc. ...
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bioRxiv - Microbiology 2023Quote: ... and the selection of inactivated cells was performed by DNA extraction using the NucleoSpin Blood kit (Macherey-Nagel) followed by PCR amplification using primers flanking the Cas9 cleavage site ...
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bioRxiv - Molecular Biology 2024Quote: ... A 200 ul aliquot was used for RNA extraction using a NucleoMag Virus kit (Macherey-Nagel, Düren, Germany) and a Magnetic Particle Processor (MPP ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 g of soil were used for DNA extraction using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and genomic DNA was extracted using the Nucleospin Blood L Midi genomic DNA extraction kit (Macherey-Nagel, 740954.20). Genomic DNA was PCR amplified by the Genetic Perturbation Platform at the Broad Institute using the following primers:
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bioRxiv - Plant Biology 2022Quote: ... undecanal-tagged peptides and excess reagents were removed by passing the reaction through HRX-reverse phase spin-columns (Macherey-Nagel GmbH & Co, KG, Dueren, Germany). The flow-through containing the enriched N-termini were dried with a SpeedVac to a remaining volume of ~50 μL ...
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bioRxiv - Molecular Biology 2021Quote: ... Fragments of 300–2000 bp were excised from the gel and isolated with a gel-extraction kit (Macherey-Nagel, NucleoSpin Gel and PCR Clean-up ...
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bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA extraction was performed on the whole guts of five workers using the NucleoSpin Soil kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: The total RNA was isolated from macrophages (infected and uninfected) using Nucleo spin RNA extraction kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: Testis DNA extraction was carried out for each individual testis piece using the NucleoMag Tissue Kit (Macherey-Nagel, #744300.1) according to the manufacturer’s conditions except for a few modifications ...
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bioRxiv - Cell Biology 2023Quote: Total RNA extraction and complementary DNA (cDNA) synthesis were performed using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany) and High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: A second purification step was performed using a PCR product extraction from gel agarose kit from Macherey-Nagel (MN) NucleoSpin® Gel and PCR Clean-up kit and restarting the above protocol from the step with the addition of CQW buffer then PW2 buffer.
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bioRxiv - Genomics 2021Quote: ... Menaquinones were then removed from the plate and analyzed using HPLC fitted with a reverse phase column (Macherey-Nagel, 2 mm x 125 mm, 3 µm, RP18) with methanol ...
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bioRxiv - Microbiology 2019Quote: Total cellular RNA from well-differentiated hAECs was extracted with the Nucleospin™ RNA extraction kit (Macherey-Nagel, Oensingen, Switzerland) according to the manufacturer’s guidelines ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA and proteins (used for the proteomic study) were extracted using the NucleoSpin® RNA/Protein extraction kit (Macherey-Nagel). RNA quantification was performed using a Nanodrop® spectrophotometer (ND-1000) ...
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bioRxiv - Cell Biology 2021Quote: ... The final transgenic BAC DNA is then transferred back to DH10b cells for better extraction yield (Nucleobond BAC100, Macherey-Nagel). DNA is resuspended in 10mM Tris-HCL pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: ... The final transgenic BAC DNA is then transferred back to DH10b cells for better extraction yield (Nucleobond BAC100, Macherey-Nagel). DNA is resuspended in injection buffer (10mM Tris-HCL pH 7.0 ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... PBS was removed by aspiration after spinning down and continued to RNA extraction with Nucleospin RNA kit (#740955.50, Macherey-Nagel) (incubated in the first buffer for 15 min after vortexing) ...
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bioRxiv - Microbiology 2020Quote: Spike peptide-stimulated splenocytes split were used for RNA extraction by using the sNucleoSpin™ Kit Plus kit (Macherey-Nagel). cDNA was generated by using a high-capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... difficile strains was done using standard methods.33 Routine DNA extractions were performed using the Nucleospin Plasmid Easypure (Macherey-Nagel) and DNeasy Blood and Tissue (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: ... Size selection on each pool was performed via gel extractions using NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL) targeting fragment sizes comprised between 250 and 500 bp ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Bacterial DNA extraction was done after 48h of fermentation using Nucleospin DNA Stool Genomic DNA Purification Kit (Macherey-Nagel, Germany) and relative abundance of selected gut bacteria was determined by qRT-PCR.
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bioRxiv - Neuroscience 2019Quote: ... RNA samples were obtained from the cell pellet using the RNA-protein extraction kit as specified by manufacturer (Macherey-Nagel). Library construction and RNA sequencing were performed by the Duke-NUS Genome Biology Facility ...
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bioRxiv - Neuroscience 2019Quote: ... high quality DNA was obtained from ear punches using a tissue DNA extraction kit according to the manufacturer’s instructions (Macherey-Nagel). One amplicon spanning approximately 1.5 kb (open arrows ...