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Citations for Macherey-Nagel GmbH :
201 - 250 of 718 citations for Saporin Chicken Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... sliced and purified with Nucleospin PCR and Gel Clean-up Kit (Macherey-Nagel). Concentration was measured in a NanoDrop device.
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bioRxiv - Cell Biology 2021Quote: ... and the mRNA was purified using a NucleoSpin RNA mini kit (Macherey-Nagel), following manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... gRNA constructs were linearized by BamHI digestion and purified by column (Macherey-Nagel). gRNA was synthesized by in vitro transcription using MEGAshortscript T7 Transcription kit (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... Subsequently the plasmid DNA was purified using a Giga Purification Kit (Macherey-Nagel). After EcoRV digestion the insert DNA was separated from the backbone DNA by PEG precipitation ...
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bioRxiv - Cell Biology 2022Quote: ... and purified using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). In vitro transcription reaction was carried out in a final volume of 100 μl transcription mix containing 80 mM HEPES (pH 7.5) ...
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bioRxiv - Developmental Biology 2021Quote: ... Reactions were purified using the NucleoSpin PCR and Gel Purification Kit (Macherey-Nagel). Samples were pooled after qPCR quantification ...
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bioRxiv - Cell Biology 2021Quote: ... purified and subjected to DNA digestion using the NucleoSpin RNA kit (MACHEREY-NAGEL). RNA concentration was measured using NanoQuant Plate™ (Infinite M200 ...
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bioRxiv - Genetics 2021Quote: Assembled constructs were purified with NucleoBond Xtra Midi Prep Kit EF (Macherey-Nagel) before injection.
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bioRxiv - Immunology 2020Quote: ... The PCR product was purified using the PCR Clean-up Kit (Macherey-Nagel) and was subsequently digested with PspXI (New England BioLabs ...
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bioRxiv - Immunology 2021Quote: ... Barcoded amplicons were pooled and purified using Nucleo Mag NGS beads (Macherey-Nagel), then sequenced using HiSeq 2500 (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Macherey-Nagel #740579) and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075).
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bioRxiv - Cell Biology 2021Quote: ... The DNA template was purified with NucleoSpin PCR Clean-up kit (Macherey-Nagel). Pre-miR-181a-1 in vitro synthesis was performed using T7 MEGAshortscriptTM kit (Ambion ...
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bioRxiv - Neuroscience 2020Quote: ... and purified using the NucleoBond Xtra Midi Plus EF (Macherey-Nagel, Hoerdt, France), according to the manufacturer’s recommendations.
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bioRxiv - Developmental Biology 2022Quote: ... The plasmids were purified using a NucleoBond Xtra Midi EF kit (Macherey-Nagel) and dissolved in TE (10 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2022Quote: ... and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). The plasmid N and inserts were digested and purified ...
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bioRxiv - Microbiology 2022Quote: ... and then purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel). Amplicons were then assembled (1:1 insert:vector molar ratio ...
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bioRxiv - Microbiology 2022Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Macherey-Nagel #740579) and were transfected into early passage NIH-3T3 cells in 6 well plates using TransIT-X2® dynamic delivery transfection system (Mirus) ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Nucleospin® extract II kit (Macherey-Nagel) according to manufacturer’s instructions and were Sanger sequenced (Eurofins UK).
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bioRxiv - Cell Biology 2023Quote: ... and purified with a silica-based resin column (MACHEREY-NAGEL, protino Ni-IDA). Aliquots were flash frozen in liquid nitrogen and stored at –80°C ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA was purified with the NucleoSpin Gel and PCR cleanup Kit (Macherey-Nagel) and used for quantitative PCR to detect gene-specific regions near the respective transcriptional start (Suppl ...
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bioRxiv - Microbiology 2023Quote: ... the purified PCR amplicons (Macherey-Nagel NucleoSpin Gel and PCR Clean-up Kit) of CJnc230 up- and downstream regions ...
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bioRxiv - Molecular Biology 2023Quote: ... purified through agarose gel electrophoresis using NucleoSpin Gel and PCR Cleanup (Macherey-Nagel), eluted in water and concentrated SpeedVac Concentrator SAVANT SPD 121P (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: Total RNA was purified by the NucleoZOL kit (740404.200, MACHEREY-NAGEL, Duren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and then purified using NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL) with Buffer NTB.
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bioRxiv - Developmental Biology 2023Quote: ... and purified with the NucleoSpin RNA Clean-up XS kit (Macherey-Nagel, 740902). The mRNA from plasmid #3 was transcribed using the mMESSAGE mMACHINE T7 Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA was purified from frozen hippocampi using NucleoSpin Tissue columns (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and plasmid DNA was extracted and purified using the NucleoSpinPlasmid kit (Macherey-Nagel). Bait proteins encoded by pIX-Halo were expressed using the TNT T7-coupled wheat germ extract system (Promega ...
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bioRxiv - Bioengineering 2023Quote: ... Pooled library plasmids were purified using NucleoBond PC 500 Maxi kit (Macherey-Nagel). The same protocol was applied to generate the library for the second round of selection ...
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bioRxiv - Biochemistry 2023Quote: ... The GST fusion proteins were purified with Protino Glutathione Agarose 4B (Macherey-Nagel) and GST was cleaved by human thrombin (Biopharm laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... The ligation was purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) and used for the electroporation of ElectroMAX DH5α-E Competent Cells (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Nucleospin® extract II kit (Macherey-Nagel) according to manufacturer’s instructions and were Sanger sequenced (Eurofins UK).
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bioRxiv - Plant Biology 2024Quote: ... The protein was purified using Protino Ni-NTA agarose (Macherey-Nagel, #7450400-500).
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bioRxiv - Genetics 2023Quote: ... DNA was purified using NucleoSpin PCR clean-up kit (Macherey-Nagel, cat# 740609), dissolved with ddH2O and adjusted to a concentration of 0.6 μM ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were purified with the NucleoSpin gel and PCR Clean-up (Macherey-Nagel).
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bioRxiv - Microbiology 2024Quote: PCR products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) according to manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were then purified with a PCR clean-up kit (Macherey-Nagel, DE), quantified with the PicoGreen assay as described above ...
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bioRxiv - Cancer Biology 2021Quote: ... The 3C template was purified using Nucleomag PCR beads (“P-beads”, Macherey-Nagel #744100.1), eluted in 450 μl 5mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was purified from 106 target cells using the Nucleospin RNA plus (Macherey-Nagel), and RT-PCR was performed using the QIAGEN OneStep RT-PCR kit according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was purified by NucleoSpin® Gel and PCR clean up (Macherey-Nagel, Bethlehem, PA) after which the nucleotide sequence determined by the Sanger method (Genewiz ...
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bioRxiv - Evolutionary Biology 2021Quote: ... purified using Nucleospin® gDNA cleanup kit from Macherey-Nagel (Hudlow et al., 2011) following the supplier’s instructions with a two-fold elution step and finally quantified with Qubit® 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Transcripts were purified using the RNA Nucleospin Clean up kit (Macherey-Nagel, Bethlehem, PA). The transcribed NeuroD1 was injected at 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... The products were then purified using the NucleoSpin PCR Clean-up kit (Macherey-Nagel). We then performed 2×250 bp paired-end MiSeq sequencing (Illumina).
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bioRxiv - Genetics 2019Quote: ... Products were then purified and size-selected using NucleoMag beads (Macherey-Nagel, Düren, Germany) for two rounds of purification/size selection according to the supplied protocol at a ratio of bead solution 1.3 times the reaction volume and eluted in 30 µl EBT ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR reactions were purified with NucleoSpin® Gel and PCR Clean-up (MACHEREY-NAGEL). Purified reactions were PCR-amplified with primers Illumina_indX_F and Illumina_indX_R (where XXXXXXXX is index ...
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bioRxiv - Synthetic Biology 2019Quote: ... The amplified fragment was purified with NucleoSpin Gel and PCR clean-up (Macherey-Nagel) and ligated with T4 DNA Ligase (Thermo-Fisher) ...
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bioRxiv - Genetics 2019Quote: ... RNAs were purified using the NucleoSpin® RNA Clean-up XS kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... PCR products were purified using Nucleospin Gel and PCR Clean-up kits (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... All plasmids were purified using NucleoBond Xtra purification kit (MACHEREY-NAGEL Inc., Bethlehem, PA).
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bioRxiv - Microbiology 2020Quote: ... Purified rbcL amplicons (NucleoSpin® gel and PCR clean-up kit, Macherey-Nagel, France) were either Sanger-sequenced directly or cloned into pGEM-T easy vector plasmids using competent Escherichia coli JM109 cells (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... gel-purified using the Nucleospin® Gel and PCR Clean-Up kit (Macherey-Nagel), and cloned into the LentiORF pLEX-MCS Vector (Thermo Scientific ...