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Citations for Macherey-Nagel GmbH :
1 - 50 of 81 citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
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bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
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bioRxiv - Biophysics 2022Quote: ... and the protein was affinity- purified from lysate using Ni-NTA affinity resin (Macherey-Nagel) and elution with imidazole ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified by affinity column purification (Macherey-Nagel). Reverse transcription was performed using Maxima Reverse Transcriptase (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... Ni-IDA affinity beads (Macherey-Nagel, Bethlehem, PA, USA) were used to purify TBC-DEG assemblies ...
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bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
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bioRxiv - Microbiology 2020Quote: ... PomXK13AR15A-His6 was affinity purified with Protino Ni-NTA resin (Macherey-Nagel) from a batch ...
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bioRxiv - Microbiology 2020Quote: ... PomXC-His6 was affinity purified with Protino Ni-NTA resin (Macherey-Nagel) from a batch ...
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bioRxiv - Microbiology 2020Quote: ... PomXN-His6 was affinity purified with Protino Ni-NTA resin (Macherey-Nagel) from a batch ...
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bioRxiv - Molecular Biology 2019Quote: ... coli (DE3) and affinity purified using Protino Ni-NTA Agarose (Macherey-Nagel). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... DNA fragments were purified using NucleoSpin (Macherey-Nagel) and diluted to 1/100 for input and to the half for immunoprecipitated fractions ...
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bioRxiv - Cell Biology 2021Quote: ... Fragments were gel purified (following kit instructions, Macherey-Nagel) and 30 ng of insert and 10 ng of vector ligated using 6U of T4 DNA ligase (Thermo ...
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bioRxiv - Microbiology 2022Quote: ... The clarified lysate was subjected to affinity chromatography on Ni2+ agarose resin (Macherey-Nagel) and washed extensively with lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... Low copy plasmids were extracted by NucleoBond Xtra Midi kit (MACHEREY-NAGEL) with the other plasmids were extracted by plasmids midi kit (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... Low copy plasmids were extracted by NucleoBond Xtra Midi kit (MACHEREY-NAGEL) with the other plasmids were extracted by plasmids midi kit (QIAGEN ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR fragments were extracted from agarose gels (Macherey-Nagel, 740609.250). pBlueRabbit vector was digested with EagI and XbaI ...
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bioRxiv - Cell Biology 2023Quote: ... The large DNA fragment was gel purified (Macherey-Nagel, 740609.50) and used as a target backbone for cloning the AviTag constructs ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged proteins in soluble extracts were purified by GSH-agarose beads (Macherey-Nagel, Düren, Germany) from which were eluted by the addition of 50 mM reduced L-glutathione (dissolved in 25 mM Tris-HCl pH 8).
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then purified from bacterial extracts by affinity chromatography using glutathione-conjugated agarose (Macherey-Nagel), followed by cleavage of the GST-tag with PreScission protease ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Microbiology 2021Quote: ... The Psyn ThyX recombinant protein was purified on Protino Ni-TED column (Macherey-Nagel) as previously described49 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM PMSF and purified by immobilized metal affinity chromatography using a 5 ml nickel column (Macherey-Nagel). The protein was eluted from the nickel column with solubilization buffer supplemented with 500 mM imidazole ...
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bioRxiv - Plant Biology 2020Quote: ... The fragment was purified using a NucleoSpin® kit (Macherey-Nagel, Düren, Germany) and cloned into the pENTR/SD/D-Topo vector (Invitrogen ...
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bioRxiv - Synthetic Biology 2020Quote: ... PCR fragments were purified using a PCR Purification Kit (Macherey-Nagel, Duren, Germany), and plasmids were purified with a Plasmid Miniprep Kit (Macherey-Nagel).
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from cell-free lysate by metal ion affinity chromatography with Protino Ni-NTA Agarose (Macherey-Nagel) as matrix followed by ultrafiltration (Amicon Ultra-15 centrifugal filter units ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins from the soluble fraction were purified by affinity chromatography with Protino Ni-TED PackedColumns2000 (Macherey-Nagel, Düren, Germany) based on the His-tag tail ...
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bioRxiv - Synthetic Biology 2019Quote: ... The amplified fragment was purified with NucleoSpin Gel and PCR clean-up (Macherey-Nagel) and ligated with T4 DNA Ligase (Thermo-Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... Infectious KSHV recombinants were generated by transfection of purified bacmid DNA (NucleoBond Xtra Midi (Macherey-Nagel)) into iSLK cells using GenJet Ver ...
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bioRxiv - Synthetic Biology 2019Quote: The amplified DNA fragments were then gel purified with NucleoSpin Extract II kit (Macherey-Nagel) and quantified ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments were purified by using NucleoSpin Gel and PCR Clean-up kits (Macherey-Nagel). Oligos were synthesized by TAG Copenhagen A/S and DNA sequences were sequenced at Eurofins Genomics ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplified fragments were purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany) and DNA concentrations were measured by NanoDrop.
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bioRxiv - Cancer Biology 2023Quote: ... then the larger linear fragment pBA439_linear was recycled and purified by columns (Macherey-Nagel, 740609.50). The ssDNA_UMI20 was assembled with pBA439_linear to construct a pBA439_UMI20 library by the NEBuilder HiFi DNA assembly (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was transferred to a fresh falcon and directly used for immobilized metal affinity chromatography (IMAC) using the Äktapure25 system with a sample pump (Cytiva) and 1ml Ni-NTA columns (Macherey-Nagel). Proteins were concentrated where necessary using Amicon filter devices with appropriate molecular weight cut-off (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... The BoNT-X/NTNH complex was purified by gravity-flow affinity chromatography on Ni-NTA agarose resin (Macherey-Nagel, Düren, Germany) in lysis buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... The His6-3C protease was then removed from the sample by immobilized metal affinity chromatography using a 1 mL Protino Ni-NTA column (Macherey-Nagel). The mTRIM66 PHD-bromo-domain protein was further purified by size exclusion chromatography using a HiLoad 16/600 Superdex 75 pg column (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA fragments were purified with NucleoSpin gel and PCR clean-up columns (Macherey-Nagel, Düren, Germany) and were Sanger- sequenced using their corresponding primers by VBC Biotech (Vienna ...
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bioRxiv - Microbiology 2022Quote: ... PCR fragments were purified with the Gel and PCR cleanup kit (Cat. n° 740609.250, Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting fragments were gel purified with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel). The 3×FLAG-tag (DYKDHDGDYKDHDIDYKDDDDK ...
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bioRxiv - Immunology 2023Quote: ... PCR fragments were cleaned up using NucleoSpin gel and PCR clean-up kit (740609.50, MACHEREY-NAGEL) according to the users’ manual and subjected to next generation sequencing (NGS) ...
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bioRxiv - Synthetic Biology 2023Quote: ... All PCR fragments were gel-purified using NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL) by following manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The linear fragments were purified on agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Following the manufacturer’s instructions ...