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Citations for Macherey-Nagel GmbH :
1 - 50 of 332 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
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bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Immunology 2023Quote: RNA was isolated from oral epithelial cells using the Nucleospin II kit (Macherey-Nagel) and treated with Turbo DNase (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Total RNA was prepared from isolated colonic epithelial cells of Hsp60Δ/ΔIEC and Hsp60fl/fl mice on day 2 with the column-based NucleoSpin RNAII kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
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jouvence, a new human H/ACA snoRNA involves in the control of cell proliferation and differentiationbioRxiv - Genetics 2020Quote: Total RNA from human cell lines were extracted using NucleoSpin RNA Plus columns (Macherey-Nagel, France), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Total RNAs from cultured human iPS cells were extracted using NucleoSpin RNA Plus XS (Macherey-Nagel). The 100 ng of total RNAs were used as starting materials to generate RNA-seq libraries with the TruSeq Stranded mRNA LT sample prep kit (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged proteins in soluble extracts were purified by GSH-agarose beads (Macherey-Nagel, Düren, Germany) from which were eluted by the addition of 50 mM reduced L-glutathione (dissolved in 25 mM Tris-HCl pH 8).
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bioRxiv - Microbiology 2021Quote: ... The Psyn ThyX recombinant protein was purified on Protino Ni-TED column (Macherey-Nagel) as previously described49 ...
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bioRxiv - Microbiology 2023Quote: ... Infectious KSHV recombinants were generated by transfection of purified bacmid DNA (NucleoBond Xtra Midi (Macherey-Nagel)) into iSLK cells using GenJet Ver ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
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bioRxiv - Plant Biology 2022Quote: ... undecanal-tagged peptides and excess reagents were removed by passing the reaction through HRX-reverse phase spin-columns (Macherey-Nagel GmbH & Co, KG, Dueren, Germany). The flow-through containing the enriched N-termini were dried with a SpeedVac to a remaining volume of ~50 μL ...
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bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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bioRxiv - Physiology 2022Quote: ... Human keratinocyte RNA was purified using NucleoSpin RNA (Macherey-Nagel, Düren, Germany). Before the RNA preparation ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA from human islets was extracted using Nucleospin RNA XS (Macherey-Nagel) three days post-transfection.
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bioRxiv - Microbiology 2022Quote: ... −20M and GLT1cc infected human liver chimeric mice using the NucleoSpin Virus kit (Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: DNA was extracted from six human fecal samples using the NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Germany), as described previously [36] ...
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bioRxiv - Immunology 2021Quote: ... apical washes of Human coronavirus 229E (HCoV-229E) and MERS-CoV-infected hAECs were extracted using the NucleoMag VET (Macherey-Nagel) according to manufacturer’s guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from snap frozen mouse or human liver tissue using NucleoSpin RNA Mini kit (Macherey-Nagel, Düren, Germany). First strand cDNA was synthesized from 1 μg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen) ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... Cells were washed with PBS once and RNA isolated from lysed cells Nucleospin RNA kit (#740955.50, Macherey-Nagel) according to manufacturer’s instructions including rDNAse treatment ...
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bioRxiv - Microbiology 2021Quote: ... or cells (NucleoSpin RNA plus kit, Macherey-Nagel) at different time post-electroporation (4 hpe – 7 dpe) ...
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bioRxiv - Cell Biology 2022Quote: Cells were lysed with Nucleozol (Macherey-Nagel, 740404) and RNA extractions were conducted according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were collected 5 days after transfection and the cell’s genomic DNA was prepared using NucleoSpin Tissue (MACHEREY-NAGEL & Co. KG).
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed with Nucleozol™ (Macherey-Nagel, 740404) and RNA extraction was conducted according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: RNA was harvested from the cells (Macherey-Nagel; 740955) and subjected to cDNA synthesis with oligo(dT ...
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bioRxiv - Cell Biology 2020Quote: ... coli DH10 cells using a BAC prep kit (Macherey-Nagel). P19 Copg1-/- cells were transfected with 1µg BAC DNA using Effectene (QIAGEN) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA purification from cultured cells and tissue (740955; Macherey-Nagel). Complementary cDNA was synthesised using Maxima first strand cDNA kit from five hundred nanograms of purified total RNA (K1641 ...
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bioRxiv - Biochemistry 2019Quote: Total RNA was extracted from HEK293T cells using NucleoZOL (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cells were collected in Nucleoprotect RNA solution (Macherey-Nagel, Dueren, Germany). Bacteria were gated as described above ...
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bioRxiv - Cell Biology 2023Quote: RNA was extracted from cells using Nucleospin RNA (Macherey-Nagel; 740955) and cDNA was synthesized from 1 μg of total RNA using the M-MLV (Invitrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified edited cells using a NucleoSpin Tissue Kit (Macherey-Nagel). The HDRT integration site was PCR-amplified from genomic DNA using Q5 High-Fidelity Polymerase (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were lysed with Nucleobond buffer set iii (MACHEREY-NAGEL) and the chromosomal DNA was purified with Sera-Mag magnetic beads (Cytiva) ...
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bioRxiv - Microbiology 2021Quote: ... half of the cells was lysed in RA1 lysis buffer (Macherey-Nagel) (“on ice”) ...
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bioRxiv - Microbiology 2020Quote: ... Total DNA was extracted from cell pellets using NucleoSpin Tissue (Macherey-Nagel) kit ...
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bioRxiv - Genetics 2019Quote: ... coli BL21-Gold cells and purified on Ni-NTA Agarose (Macherey-Nagel). Two guinea pigs were immunized at Bioscience with His-DBN-1(1-132 ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was collected from cells using the NucleoSpin RNA Kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: RNA from mESC and HEK293T cells was extracted with NucleoZOL (Macherey-Nagel); from MCF7 cells BIO TRI RNA reagent (Bio-lab ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then treated with RNase (Macherey-Nagel, Thermo Fisher Scientific), stained with propidium iodide (PI ...
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bioRxiv - Biophysics 2022Quote: ... coli MC1061 cell line using the Nucleobond Xtra Maxi Kit (Macherey-Nagel). 1.3 μg DNA was used per 106 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and processed using the NucleoSpin RNA kit (Macherey-Nagel) or the PureLink RNA Mini Kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was isolated from cell lines using NucleoSpin RNA Kit (Macherey-Nagel) and subjected to Real-Time PCR using the high-capacity cDNA reverse transcription kit (Applied Biosystems ...