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Citations for Macherey-Nagel GmbH :
251 - 269 of 269 citations for Rat NDUFS4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Plasmid purifications for mammalian cell lines transfection were performed using high-yield column-based commercial NucleoBond Xtra Midi kit (Macherey-Nagel, Germany) following usage protocols.
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were grown in Luria-Bertani Broth at 37°C and plasmid DNA was isolated using the Nucleobond Xtra midi kit (MACHEREY-NAGEL, 740410), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... or 1.2 l LB-medium and incubated for 4 h at 37 °C before extracting plasmid DNA using Nucleobond Xtra Midi kit (Macherey-Nagel, C640003) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2023Quote: ... The resulting constructs were used for protoplast transformation through midiprep using the NucleoBond Xtra plasmid kit (Macherey-Nagel GmbH & Co.KG, Duren, German) as per the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... 700ng of the plasmid library was isolated from the liquid culture using the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Biochemistry 2023Quote: ... consecutive copies of 197 bp with modified 601 sequence were transformed into DH5α Escherichia coli strain and purified from four liters of culture by using plasmid DNA purification kit Nucleobond® PC 10000 (Macherey-Nagel). Three mg of the plasmids were digested with EcoRI HF (0.25 U/µg DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... these cultures were incubated overnight (800 RPM, 37°C) and miniprepped the following day using a 96 Plasmid NucleoSpin kit (Macherey-Nagel #740625.1). The resulting plasmids were able to be sequenced verified for proper VH and VL insertion ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... All colonies were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in water ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... All colonies were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in 20 µL 1X TE buffer ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in 20 µL water ...
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bioRxiv - Molecular Biology 2020Quote: ... The overnight culture was used to inoculate a 500 mL LB with the antibiotic and the plasmid DNA was isolated using NucleoBond Xtra Midi EF (Macherey-Nagel, Dueren, Germany). Sequence flanking the spacer sequences were amplified and were sequenced ...
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bioRxiv - Physiology 2021Quote: ... Ak1 cDNA and plasmids were digested with SpeI and BglII and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Ligation was performed using Ligation High Ver ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... Correct clones were determined by sequencing and cultured for subsequent plasmid preparation using NucleoBond Xtra Midi/Maxi kits (Macherey-Nagel GmbH & Co. KG). For scaled CFPS reactions ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid DNA was isolated with NucleoSpin Plasmid Mini Kit and extracted from an agarose gel with NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Duren, Germany). All constructs were verified by restriction digest and Sanger sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was isolated with NucleoSpin Plasmid Mini Kit and extracted from an agarose gel with NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel, Duren, Germany). All constructs were verified by restriction digest and Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... If no genomic DNA could be obtained strains were cultured according to the manufacturer’s protocol and DNA was extracted using the NucleosSpin Plasmid (NoLid) Mini kit (Macherey-Nagel cat. No. 740499) with vortexing after addition of BufferA2 and BufferA3 ...
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bioRxiv - Bioengineering 2024Quote: ... The constructs were assembled into the AAV2-ITR plasmid via molecular cloning and purified using the NucleoBond Xtra Maxi EF kit (Macherey-Nagel, Düren, Germany). The purified plasmids were sent to Vigene Biosciences (now Charles River ...
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bioRxiv - Genomics 2022Quote: ... After 20 hours at 30 ºC bacteria were harvested and plasmid preparations were performed using Macherey-Nagel™ NucleoBond™ Xtra Midi EF (Macherey-Nagel #740420.50).
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bioRxiv - Developmental Biology 2022Quote: ... All plasmids were cloned into the DH5a strain (Enzynomics) and purified with a NucleoBond Xtra Midi Plus kit (MACHEREY-NAGEL GmbH & Co. KG, Dueren, Germany) according to the manufacturer’s instructions.