Labshake search
Citations for Macherey-Nagel GmbH :
51 - 100 of 269 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... Plasmids were extracted from various colonies with the NucleoSpin plasmid mini-kit (740588.50, Macherey-Nagel). Validated plasmids were then sequenced by Eurofins.
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmid DNA was extracted from pelleted cells using minipreps (NucleoSpin Plasmid EasyPure Kit - Macherey-Nagel) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from 1 mL overnight culture using NucleoSpin Plasmid Easypure columns (Macherey-Nagel) from the Plasmid Easypure Kit (Nucleospin).
-
bioRxiv - Developmental Biology 2024Quote: ... and resistant clones then used for miniprep plasmid preparations (Nucleospin plasmid, Macherey-Nagel, Cat # 740588.50), followed by sequencing ...
-
bioRxiv - Molecular Biology 2019Quote: ... grown in liquid culture overnight and plasmids were extracted using the NucleoSpin Plasmid Kit (Macherey-Nagel) according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... cultures were subjected to plasmid isolation using the NucleoSpin® Plasmid EasyPure Kit (Macherey-Nagel, Germany) or colony PCR using Q5® High-Fidelity 2× Master Mix (New England Biolabs ...
-
bioRxiv - Biochemistry 2021Quote: This plasmid was amplified and purified using the NucleoBond PC 500 Plasmid Maxiprep Kit (MACHEREY-NAGEL). P107M612-S687 was subcloned into an MBP-fusion vector (pTHMT) ...
-
bioRxiv - Microbiology 2020Quote: Plasmid DNA was isolated from DH5α using the Nucleospin Plasmid kit (MACHEREY-NAGEL Inc., Bethlehem, PA) and transformed into the DAP-auxotroph donor strain E ...
-
bioRxiv - Synthetic Biology 2023Quote: ... or NucleoSpin Plasmid kit (Macherey-Nagel). The correct assembly of all plasmids was verified by Sanger sequencing (Microsynth Seqlab).
-
bioRxiv - Microbiology 2024Quote: ... or Nucleospin Plasmid Kit (Macherey-Nagel). DNA-modifying enzymes and restriction enzymes were purchased from New England Biolabs ...
-
bioRxiv - Genetics 2021Quote: ... coli (High Efficiency) cells and subsequent plasmid isolation was performed using the NucleoSpin Plasmid kit (Macherey-Nagel).
-
bioRxiv - Microbiology 2022Quote: ... Plasmids from selected positive clones were extracted and purified on silica columns (NucleoSpin® Plasmid, Macherey-Nagel™), then sequenced with their specific oligonucleotides (Eurofins Genomics) ...
-
bioRxiv - Microbiology 2022Quote: ... Cloning was performed according to standard protocols and plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel). DNA fragments were amplified using Phusion High Fidelity DNA polymerase (NEB ...
-
bioRxiv - Cell Biology 2021Quote: ... and NucleoSpin® Plasmid (Macherey-Nagel #740499). All the entry vectors were sequenced ...
-
bioRxiv - Microbiology 2021Quote: ... before plasmid extraction by miniprep (Macherey-Nagel). Electrocompetent cells of MG1655 carrying PARIS from E ...
-
bioRxiv - Biochemistry 2021Quote: ... Mini kit for plasmid DNA (Macherey-Nagel). To obtain linear DNA of the indicated length ...
-
bioRxiv - Microbiology 2022Quote: ... The nucleoSpin plasmid easypure Kit (Macherey-Nagel) was used for plasmid purification and expression cassettes were sequence verified ...
-
bioRxiv - Biophysics 2023Quote: ... Mini kit for plasmid DNA (Macherey-Nagel). Correct mutation of the plasmids was verified by sequencing performed by MicroSynth GmbH Göttingen.
-
bioRxiv - Microbiology 2023Quote: ... NucleoSpin Plasmid EasyPure Kit (Macherey-Nagel, Germany) was used for plasmid preparation ...
-
bioRxiv - Molecular Biology 2024Quote: ... the NucleoSpin Plasmid Mini kit (MACHEREY-NAGEL) was used for minipreparations ...
-
bioRxiv - Cell Biology 2020Quote: ... All plasmids were purified from 4 ml bacterial culture using either NucleoSpin® Plasmid (REF 740588.50, Macherey-Nagel®) or PureLink™ HiPure Plasmid Miniprep Kit (Art ...
-
bioRxiv - Bioengineering 2023Quote: ... Library plasmids were harvested from transformed bacteria and purified using the NucleoBond Xtra MIDI plasmid preparation kit (Macherey-Nagel). The AAV library genomes were packaged into 293T cells by transfecting 1 × 107 in ten 15-cm with the library plasmids (containing the cap gene with peptide insertion ...
-
bioRxiv - Immunology 2020Quote: ... Positive clones were identified by colony PCR and the plasmid was purified using NucleoSpin® Plasmid EasyPure kit (Macherey-Nagel). The cloned devil NLRC5 transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI) ...
-
bioRxiv - Biochemistry 2023Quote: ... Plasmids were then purified using the NucleoBond PC 10000 EF Giga kit for endotoxin-free plasmid DNA from Macherey-Nagel. In parallel ...
-
bioRxiv - Bioengineering 2020Quote: ... NucleoSpin® Plasmid EasyPure Kit (Macherey-Nagel, Germany) was used for plasmid preparation ...
-
bioRxiv - Microbiology 2021Quote: ... using the NucleoSpin Plasmid Mini Kit (Macherey-Nagel) according to manufacturer’s protocol ...
-
bioRxiv - Biophysics 2022Quote: ... Plasmid preparation kits were purchased from Macherey-Nagel.
-
bioRxiv - Synthetic Biology 2023Quote: ... and plasmid kits were purchased from Macherey-Nagel. Precision Plus ProteinTM protein standard was provided by Biorad ...
-
bioRxiv - Neuroscience 2023Quote: ... coli was done using NucleoSpin Plasmid (MACHEREY-NAGEL). The mutations were verified by sequencing (Microsynth ...
-
bioRxiv - Biochemistry 2024Quote: ... Plasmid preparation kits were purchased from Macherey-Nagel.
-
bioRxiv - Microbiology 2021Quote: ... 107 cells were inoculated in 50 ml LB medium supplemented with 100 µg/ml spectinomycin and grown overnight at 37 °C for purification of the plasmid library DNA by miniprep (NucleoSpin Plasmid, Macherey-Nagel). The efficiency of fragment insertion (100% ...
-
bioRxiv - Molecular Biology 2020Quote: ... the total genomic DNA from plasmids was extracted from fresh transformed bacteria using a plasmid DNA Maxi Prep kit (Nucleobond XtraMaxi EF, Macherey-Nagel). Transgenic control GFP fluorescent marker expressing line (NB30 ...
-
bioRxiv - Microbiology 2021Quote: ... selected clones were grown overnight at 37°C under agitation and the plasmid DNA was extracted and purified using the NucleoSpin plasmid Mini or Midi kit (Macherey-Nagel).
-
bioRxiv - Genomics 2019Quote: ... One colony per construct was grown overnight in a shake incubator at 37°C before plasmid extraction using the NucleoSpin Plasmid Transfection-grade kit (Macherey-Nagel). For each construct three biological replicates were performed by transfecting the plasmids into 80% confluent cells of MM001 in a 24 well plate ...
-
bioRxiv - Plant Biology 2022Quote: ... Plasmids were extracted from positive colonies using 5 mL liquid cultures with the NucleoSpin Plasmid Purification kit (Macherey-Nagel; Ref.: 11932392) and sequenced (GATC ...
-
bioRxiv - Bioengineering 2023Quote: ... supernatant was removed and cell pellets were used for plasmid isolation by using NucleoBond□ Xtra Midi Plasmid DNA Purification Kit (Macherey-Nagel) according to the protocol supplied by the kit.
-
bioRxiv - Immunology 2021Quote: ... Positive clones were identified by colony PCR and the plasmids were isolated using NucleoSpin® Plasmid EasyPure kit (Macherey-Nagel, Düren, Germany). The DNA sequence of the cloned devil CIITA transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmid DNA was purified from the remaining cultures using a NucleoSpin Plasmid (NoLid) high-pure plasmid mini prep kit (cat. no. 740499.50, Macherey-Nagel, Bethlehem, PA) according to vendor instructions and quantified using the Qubit dsDNA BR Assay (cat ...
-
bioRxiv - Microbiology 2020Quote: ... coli using NucleoSpin Plasmid Kit (Macherey-Nagel, Düren, Germany). Deletion of the degU ...
-
bioRxiv - Microbiology 2020Quote: ... the NucleoSpin® Plasmid kit (Macherey-Nagel, Düren, Germany) was used ...
-
bioRxiv - Genomics 2021Quote: ... using the NucleoSpin Plasmid EasyPure kit #740727250 (Macherey-Nagel), according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... coli cultures using NucleoBond Plasmid MAXI KIT (Macherey-Nagel) and resuspended in TE buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmid DNA was purified by NucleoSpin column (Macherey-Nagel), then analysed on a 1% agarose gel containing RedSafe™ (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA was purified with NucleoBond (Macherey-Nagel, Germany). 2A encodes for a ribosomal skip sentence ...
-
bioRxiv - Microbiology 2023Quote: ... Mini kit for plasmid DNA (Macherey-Nagel, Cat. # 740588) or Plasmid Plus Maxi Kits (QIAGEN ...
-
bioRxiv - Microbiology 2023Quote: ... pLC10 purification was performed with Plasmid EasyPure (Macherey-Nagel).
-
bioRxiv - Plant Biology 2023Quote: ... performed with the NucleoSpin Plasmid kit (Macherey-Nagel, Germany); the successful cloning was verified by Sanger sequencing.
-
bioRxiv - Cell Biology 2021Quote: ... Plasmids were purified using Plasmid DNA Mini Kit I (Omega Biotech, cat# D6943-02) or Nucleobond Xtra Mid kit (Macherey-Nagel, cat# 740410.50), according to protocols supplied by the manufacturers ...
-
bioRxiv - Molecular Biology 2019Quote: ... Starting from overnight grown liquid cultures of respective pAGM9121 constructs (prepared as described in section 5) plasmid mixtures were prepared using a NucleoSpin Plasmid Kit (Macherey-Nagel, Düren, DE). Following the preparation ...
-
bioRxiv - Molecular Biology 2024Quote: ... All plasmids were maintained in Escherichia coli and purified with a standard plasmid midi-prep kit (NucleoBond® Xtra Midi, MACHEREY-NAGEL, DE).