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Citations for Macherey-Nagel GmbH :
701 - 750 of 778 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Products were run on 1% agarose gels and then gel-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... The PCR products of correct size were purified by agarose gel electrophoresis (NucleoSpin Gel and PCR Clean-up Kit, Macherey-Nagel). The purified linear DNA was used for cloning of pTS040 by Gibson assembly (NEBuilder HiFi DNA Assembly Reaction ...
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bioRxiv - Biochemistry 2023Quote: ... The BoNT-X/NTNH complex was purified by gravity-flow affinity chromatography on Ni-NTA agarose resin (Macherey-Nagel, Düren, Germany) in lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... was added on top of the bone powder and total RNA were purified with Nucleospin RNA set nucleozol column (Macherey-Nagel) according to the manufacturer recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR product was run on a 1% agarose gel and the correct band according to size was purified using the NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products were visualized on a 1% TAE agarose gel and purified using the Nucleospin PCR Clean-up Kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were scraped with 30-50 mL Luria-Bertani (LB) broth and plasmids were purified with Endotoxin-Free MaxiPrep kit (Macherey-Nagel). Precipitated DNA was resuspended in 1 mL water and the concentration was measured at Nanodrop ...
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bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
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bioRxiv - Microbiology 2023Quote: ... and 16S amplicons (500 bp amplicon size) were purified by agarose gel separation using the NucleoSpin Gel and PCR Clean-up purification kit (Macherey-Nagel). Bacterial 16S amplifications were obtained using the FastStart High-Fidelity PCR system (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was separated from the debris by centrifugation at 4000g for 40 minutes at 4°C and the supernatant was purified using 1 ml Ni-NTA columns (Macherey-Nagel). His-tagged UL12 was eluted using lysis buffer with increasing concentration of Imidazole (100 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... pX458 plasmid was digested with BpiI (BbsI; Thermo, #ER1011) and gel-purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel); gRNA oligonucleotides were ligated into pX458 plasmid with T4 DNA ligase (NEB ...
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bioRxiv - Immunology 2023Quote: RNAs from whole lungs or BM of naive or IAV-infected mice were extracted and purified using the NucleoSpin RNA Extraction Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids were propagated in cultures of terrific broth medium with appropriate antibiotics overnight at 37 °C and purified with NucleoSpin miniprep kit (Macherey-Nagel) following the manufacturer’s prototol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Three independent PCR reactions from the same library were combined after PCR and purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Sequencing was carried out on an Illumina HiSeq XTen at Macrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... All PCR or vector digestion products were purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel; #740609.50). The final plasmids were transformed into competent E.coli XL1-Blue ...
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bioRxiv - Genomics 2024Quote: ... dissolved in TE buffer (10 mM Tris pH8, 1 mM EDTA pH8) and purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel). DNA was loaded on a 2% agarose gel ...
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bioRxiv - Genomics 2024Quote: ... Cross-linking was reversed by overnight incubation at 65°C and DNA fragments were purified using NucleoSpin Gel and PCR Clean-up columns (Macherey-Nagel).
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bioRxiv - Neuroscience 2022Quote: RNA from purified astrocytes or brain cell suspension after enzymatic digestion was extracted using RNA-binding columns (NucleoSpin RNA plus Macherey-Nagel #740984.250), genomic DNA was eliminated and cDNA was synthetised (Thermo Fisher Scientific #K1672) ...
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bioRxiv - Microbiology 2021Quote: ... P1-P4 were purified P1-P4 containing cultures by solid phase extraction with reversed phase C18 columns (Chromabond, Macherey-Nagel, Düren, Germany). Supernatants were acidified to pH 3 with HCl ...
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bioRxiv - Genomics 2021Quote: ... embryos were ground in Tri-Reagent and RNA was purified using affinity chromatography (Nucleospin RNA Clean up kit, Macherey-Nagel, Duren, Germany). Potential contaminating DNA was removed by digestion with rDNase then RNA was purified using Beckman Coulter’s solid-phase reversible immobilization (SPRI ...
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bioRxiv - Cell Biology 2021Quote: ... Eluates were digested with proteinase K and RNAse A and DNA fragments were purified using a PCR purification kit (Macherey-Nagel, #740609).
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bioRxiv - Developmental Biology 2021Quote: ... and the band with the expected size excised and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). The second PCR products were sent for Sanger sequencing to Eurofins Genomics and checked for quality ...
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bioRxiv - Microbiology 2020Quote: ... Polymerase chain reaction (PCR) products and plasmid digestion derivatives were purified using NucleoSpin® Gel and PCR Clean-up kits (Macherey-Nagel). Plasmids were extracted from E ...
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bioRxiv - Microbiology 2019Quote: ... All single fragment PCR reactions were realized using Phusion High-Fidelity DNA Polymerase (ThermoScientific) and PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). Overlap PCRs were carried out using 100 ng of each purified PCR products and the resulting fragment of interest was purified from agarose gel ...
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bioRxiv - Microbiology 2020Quote: ... to reach at least 200x colonies per guide-RNA and the library plasmid pool was purified using NucleoBond Xtra-Maxi Kit (Macherey-Nagel, #740414.50). Then ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting PCR product was purified using a standard PCR purification kit (NucleoSpin® Gel and PCR Clean-up kit, Macherey-Nagel) and used as a template for the second PCR that used the gene-specific forward primer and a 3’ T7 universal reverse primer targeting the forward linker sequence for the antisense probe ...
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bioRxiv - Immunology 2021Quote: ... The amplified PCR product is then purified from gel using the “Nucleospin Gel and PCR Clean-up” kit (Macherey-Nagel, Reference: 740609.250).
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were realized using Phusion High-Fidelity DNA Polymerase (ThermoScientific) and PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). The overlap PCR product was purified from agarose gel and was directly used to transform wild type F ...
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bioRxiv - Developmental Biology 2022Quote: ... plasmids were linearized by AscI (pBSK vector) or SfiI (pYX vector) and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, #740609). cRNA was produced by in vitro transcription using mMessage mMachine™ T3 kit (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was amplified by PCR methods using the following oligo-DNA primers and the products were purified with micro spin columns (MACHEREY-NAGEL, 74060910). Each reverse primer also possesses T3 sequence for transcription.
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bioRxiv - Plant Biology 2023Quote: ... The two resulting PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Ref. REF 740609.50; Macherey-Nagel, Düren, Germany), digested at 37°C overnight using FastDigest Eco31I (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products from the same sample were pooled and purified over 2% agarose gels (band size at 275 bp) using NucleoSpin® Gel and PCR clean up kit (Macherey-Nagel). Purified amplicons were quantified by Qubit (Thermofisher ...
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bioRxiv - Genetics 2023Quote: ... was added for 30 min at 37°C and DNA was purified using NucleoSpin Gel and PCR Clean-Up columns (Macherey-Nagel, #740609.250) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR reaction was performed on the new synthetized vector to amplify the HA1-DCK*-P2A-GFP-HA2 sequence corresponding to the HDR (homology directed repair) DNA template and was purified using the NucleoSpin PCR Clean-up kit (Macherey-Nagel # 740609.10) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of the sample with gRNA–TAR and –p24 combined was run on a 1% agarose gel containing ethidium bromide and the two expected bands were cut out separately and purified using the Nucleospin® Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2023Quote: ... were then extracted from the agarose gel and the DNA purified following the manufacturer instruction (NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL)) ...
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bioRxiv - Biochemistry 2023Quote: ... consecutive copies of 197 bp with modified 601 sequence were transformed into DH5α Escherichia coli strain and purified from four liters of culture by using plasmid DNA purification kit Nucleobond® PC 10000 (Macherey-Nagel). Three mg of the plasmids were digested with EcoRI HF (0.25 U/µg DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The three constructs were then cultured for 24 h at 37°C and purified with NucleoBond Xtra Midi Kit (Macherey-Nagel, Germany) and used for gene reporter assay.
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bioRxiv - Cancer Biology 2024Quote: ... The resulting amplicons were purified using the NucleoSpin gel and PCR cleanup kit following the manufacturer’s instructions (Macherey-Nagel Inc., Bethlehem, PA) and submitted for Sanger sequencing with the same primers used to generate the amplicons ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... and we collected bands containing arrays ≥2-plex at 550-650 bps and 650-800 bps to be purified separately using a NucleoSpin Gel Purification Kit (Macherey-Nagel 740609.250). We also collected the cut vector backbone ...
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bioRxiv - Microbiology 2023Quote: ... The dephosphorylated pEX18Tc vector and PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). TEDA reaction was then carried out by mixing 150 ng of pEX18Tc with the corresponding PCR products at a molar ratio of 1:4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The NaOH was neutralized by the addition of 1 M HCl and the cDNA was purified by a PCR-clean-up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Germany) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... GRANTA-519 and B-cells purified from four MCL patients using the NucleoSpin® RNA II kit according to the manufacturer’s protocol (Macherey-Nagel, Oensingen, Switzerland). A minimum of 2µg per samples were used for analysis ...
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bioRxiv - Bioengineering 2019Quote: ... genomic fragments containing the gRNA target site were amplified with PCR and purified using NucleoSpin Gel and a PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Then 200 ng of each of the PCR products derived from the transfected and non-transfected cells were denatured at 95° C for 5 min and reannealed ...
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bioRxiv - Plant Biology 2021Quote: ... The amplicons were purified from the PCR reagent tube via the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were purified using Plasmid DNA Mini Kit I (Omega Biotech, cat# D6943-02) or Nucleobond Xtra Mid kit (Macherey-Nagel, cat# 740410.50), according to protocols supplied by the manufacturers ...
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bioRxiv - Microbiology 2021Quote: ... was linearized by AdeI restriction enzyme digestion and DNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). For in-vitro transcription ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and products of the expected size were cut out and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Duren, Germany). 10 ng of purified PCR product was then used as template for RNA probe synthesis ...
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bioRxiv - Microbiology 2021Quote: The qPCR product for hsv1-miR-H27 was first subjected to agarose gel electrophoresis and the band complementary to the size of cDNA was excised and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Germany). The purified product was cloned into T&A™ cloning vector by using T&A™ Cloning Vector (Yeastern Biotech Co. ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were analysed using 1.5% agarose gel electrophoresis in 1x Tris-borate-EDTA (TBE) buffer and purified from the gel using a NucleoSpin® gel and PCR clean-up kit (Macherey-Nagel, Germany).