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Citations for Macherey-Nagel GmbH :
501 - 550 of 724 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel, 740609.50). DNA amount was measured using a Nanodrop ...
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bioRxiv - Microbiology 2022Quote: ... whole transformation populations were recovered and their genomic DNA was isolated using Nucleospin tissue kit (Macherey-Nagel). Deletions were confirmed by two PCR reactions using primers outside of the PCR product and primers specific to the antibiotic marker used (F0-MR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Total DNA was extracted using the Nucleospin® plant II kit (Macherey-Nagel GmbH & Co., Düren, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Physiology 2020Quote: ... Genomic DNA from 32 MPM cell lines was extracted with Nucleospin Blood kit (Macherey-Nagel, Düren, Germany) and 500 ng were hybridized to Affymetrix CytoScanHD Arrays (Affymetrix ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragments were then purified with a NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel). The integrity of the purified DNA fragments was confirmed by 2% (w/v ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 5×106 B16F10 tumor cell lines using NucleoSpin® Tissue (MACHEREY-NAGEL), according to manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... DNA was extracted from ten individuals (males and females) using NucleoSpin Tissue XS kit (Macherey-Nagel, Germany) for each DNA-library ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using Nucleospin® 96 Tissue Kit according to the manufacturer’s protocol (Macherey-Nagel, Germany). Individual double-digest RAD-seq libraries were constructed with PstI and MseI according to the protocol detailed in Brelsford ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we extracted DNA from liver tissue of a single mouse using a Nucleospin Tissue Kit (Macherey-Nagel) and quantified the DNA with a QuantiFluor dsDNA System (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... Protino® Ni-NTA Agarose and NucleoBond® Midi Plasmid DNA Purification Kit were from Macherey-Nagel, Germany ...
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bioRxiv - Evolutionary Biology 2021Quote: ... total DNA was extracted using either the NucleoSpin® Plant II Midi Kit (Macherey-Nagel, Düren, Germany) or a modified CTAB protocol (Rogers and Bendich 1985 ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was extracted as pooled libraries using NucleoBond Xtra Maxi EF kit (Macherey-Nagel, Duere, Germany) and stored at −20 ℃.
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from iPSCs and EBs using the NucleoSpin Tissue Kit (Macherey-Nagel, Düren, Germany) with the manufacturer’s instructions and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Genetics 2022Quote: ... Gametophyte DNA was extracted using the Macherey-Nagel NucleoSpin Plant II Maxi Kit (Macherey-Nagel, Duren, Germany) with a modified protocol ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used for cloning reactions were purified by using the Nucleospin gel extraction kit (Macherey-Nagel) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2023Quote: ... The genomic DNA used for WGBS was prepared using a NucleoSpin Tissue Kit (Macherey-Nagel, Dueren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and reverse-crosslinked DNA was subsequently purified using isopropanol and magnetic beads (Macherey-Nagel NucleoMag PCR Beads). Purified DNA was sheared to 300-500 bp with Covaris and subsequently size-selected by AMPure XP (Agencourt) ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was extracted from cells corresponding to 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel).
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bioRxiv - Biochemistry 2023Quote: ... Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel, 740609.50). DNA amount was measured using a Nanodrop ...
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bioRxiv - Genomics 2023Quote: ... DNA purification was carried out by adding a 2 ml mixture of wash buffer PW2 (MACHEREY-NAGEL), wash buffer B5 (MACHEREY-NAGEL) ...
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bioRxiv - Microbiology 2024Quote: Total genomic DNA was extracted using the NucleoMag® Tissue extraction kit (Macherey-Nagel™, Düren, Germany) and the V3-V4 region of the 16S rDNA gene was amplified with the prokaryotic primers 343F (5′-ACGGRAGGCAGCAG – 3′ ...
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bioRxiv - Molecular Biology 2024Quote: ... and DNA was purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel; #740609.50) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were supplemented with 20 mM imidazole and incubated with Protino Ni-NTA agarose beads (Macherey-Nagel) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from cell-free lysate by metal ion affinity chromatography with Protino Ni-NTA Agarose (Macherey-Nagel) as matrix followed by ultrafiltration (Amicon Ultra-15 centrifugal filter units ...
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bioRxiv - Biochemistry 2021Quote: ... and the GST fusion proteins were purified using Protino gluthathione agarose 4B according to the manufacturer’s instruction (Macherey-Nagel). SPR interaction studies were performed according to previous studies46,85 ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of plants 5 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins from the soluble fraction were purified by affinity chromatography with Protino Ni-TED PackedColumns2000 (Macherey-Nagel, Düren, Germany) based on the His-tag tail ...
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bioRxiv - Molecular Biology 2024Quote: ... Freezing and RNA isolation was done following the instructions of the NucleoSpin RNA/Protein Kit (Macherey-Nagel; Düren, Germany). 1 µg of the extracted total RNA per sample was reverse transcribed using oligo(dt)18 primers (First Strand cDNA Synthesis Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA from individual embryos was isolated with the NucleoSpin® Tissue XS kit (Cat#740901, Macherey-Nagel). A PCR reaction was performed using the isolated genomic DNA with primers flanking the CAS9 target site ...
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bioRxiv - Genetics 2021Quote: Genomic DNA from 5 males was extracted using the NucleoSpin Tissue XS kit (Macherey-Nagel, ref #740 901). To amplify the Rsp satDNA region ...
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bioRxiv - Neuroscience 2021Quote: ... High yield of plasmid DNA was obtained using the NucleoBond® Xtra Maxi Plus EF (Macherey-Nagel, 740426.50). 70μg AAV packaging plasmid ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted DNA from fresh spores stored at -20°C using the Nucleospin Soil Kit (Macherey-Nagel, Germany). Genomes were sequenced using Illumina 150 bp paired-end sequencing technology at 46X coverage on average ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA (gDNA) was isolated from sorted and unsorted cells using the Nucleospin Tissue Mini Kit (Macherey-Nagel) and sgRNA sequences were retrieved by a nested PCR approach that pre-amplified sgRNAs in a first round with primers specific to the lentiGuide-Puro construct (5’-AGAGGGCCTATTTCCCATGA-3’ ...
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bioRxiv - Immunology 2022Quote: ... The plasmid DNA was extracted from the bacterial cultures using NucleoBond™ Xtra Midi Plus (Macherey-Nagel, 740412.10) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... Endotoxin-free plasmid DNA was purified from clones with correct sequences (Nucleobond Xtra Maxi EF, Macherey-Nagel Inc.), and re-suspended in endotoxin-free water.
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bioRxiv - Biochemistry 2019Quote: ... The eluted DNA samples were further purified with a NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL). The DNA libraries were prepared using a SMARTer ThruPLEX Tag-seq Kit (Takara Bio) ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were purified with a NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Restriction enzymes and T4 DNA ligase were obtained from Thermo Fisher Scientific and were used according to the supplier’s specifications ...
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bioRxiv - Genetics 2020Quote: DNA was extracted from blood samples using the NucleoSpin Blood kit (Macherey-Nagel, GmbH & Co. KG, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Nucleic acid extraction was performed with the NucleoSpin DNA Insect Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The final plasmid library DNA was isolated from the culture using the Nucleobond Xtra Maxi kit (Macherey-Nagel).
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from 2.5×105 HEK293T cells was purified using a Nucleo Spin Tissue XS kit (MACHEREY-NAGEL). Using 25 mg of human postmortem tissue ...
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bioRxiv - Bioengineering 2022Quote: ... DNA was purified from PCR mixtures or agarose gels using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). The purity and concentration of DNA were checked by NanoDrop 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: Large-scale purification of BAC/YAC plasmid DNA was done using a NucleoBond® Xtra BAC (Macherey-Nagel) kit ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used in cloning reactions were purified using the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the DNA of HEK293 cells was extracted and purified following the NucleoSpin Tissue protocol (Macherey-Nagel, Düren, Germany). The genotype at rs1047626 was verified to be heterozygote (AG ...
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bioRxiv - Neuroscience 2023Quote: ... genomic DNA was extracted from a single F1 mosquito using the NucleoSpin Tissue kit (Macherey-Nagel Inc., 740952.50). A diagnostic PCR was then performed to fully amplify the Act5C-ECFP marker using the primer ...
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bioRxiv - Molecular Biology 2023Quote: DNA samples was extracted from different salmon tissues using the NucleoSpin® Tissue kit (Macherey-Nagel, no. 740741) according to the manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: ... The DNA donors were then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and stored at -20°C or directly used for electroporation ...
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bioRxiv - Genomics 2023Quote: High molecular weight DNA was extracted from blood cells using a NucleoBond AXG column (Macherey-Nagel, Düren, Germany), which was followed by purification with phenol-chloroform ...