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Citations for Macherey-Nagel GmbH :
51 - 70 of 70 citations for Protein Conjugation Labeling since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... After proteolytic cleavage the protein was incubated with 1.5 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) for 25 min at 4°C to remove His6-SUMO (for purification of SUMO-Hsc70 overnight digestion with Ulp1 and second incubation with Protino Ni-IDA was omitted) ...
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bioRxiv - Molecular Biology 2021Quote: Complementary DNAs were obtained by reverse transcription of total RNA using the NucleoSpin RNA/Protein kit (Macherey-Nagel) and used as templates for PCR amplification using Brilliant III Ultra-Fast QPCR Master Mix (Agilent ...
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bioRxiv - Genetics 2020Quote: ... RNA and protein were simultaneously extracted from pellets using the NucleoSpin TriPrep Kit (Macherey-Nagel, supplied by ThermoFisher). Deletion of the target region was confirmed by end-point of DNA using primers flanking the guides (primers 5’-AGGGGTTGAGCTGGATTCC-3’ and 5’-CCAGCACCCACTCTGTAGAT-3’ ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total mRNA was extracted from leaf tissue with a NucleoSpin RNA/Protein kit (REF740933, Macherey-Nagel GmbH & Co.), then treated with DNase before transcription into cDNA using a Superscript III First-Strand Synthesis System (18080-051 ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were run on bis-acrylamide gels and then transferred to PVDF membranes (Macherey-Nagel, Düren, Germany) using a Trans-blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were supplemented with 20 mM imidazole and incubated with Protino Ni-NTA agarose beads (Macherey-Nagel) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from cell-free lysate by metal ion affinity chromatography with Protino Ni-NTA Agarose (Macherey-Nagel) as matrix followed by ultrafiltration (Amicon Ultra-15 centrifugal filter units ...
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bioRxiv - Biochemistry 2021Quote: ... and the GST fusion proteins were purified using Protino gluthathione agarose 4B according to the manufacturer’s instruction (Macherey-Nagel). SPR interaction studies were performed according to previous studies46,85 ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of plants 5 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins from the soluble fraction were purified by affinity chromatography with Protino Ni-TED PackedColumns2000 (Macherey-Nagel, Düren, Germany) based on the His-tag tail ...
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bioRxiv - Molecular Biology 2024Quote: ... Freezing and RNA isolation was done following the instructions of the NucleoSpin RNA/Protein Kit (Macherey-Nagel; Düren, Germany). 1 µg of the extracted total RNA per sample was reverse transcribed using oligo(dt)18 primers (First Strand cDNA Synthesis Kit ...
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bioRxiv - Neuroscience 2019Quote: ... RNA samples were obtained from the cell pellet using the RNA-protein extraction kit as specified by manufacturer (Macherey-Nagel). Library construction and RNA sequencing were performed by the Duke-NUS Genome Biology Facility ...
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bioRxiv - Biochemistry 2023Quote: Pull-down experiments using GST (Glutathion-S-Transferase)-fused proteins were performed using glutathione agarose beads (Macherey-Nagel, Düren, Germany). The beads were incubated with the GST-fused proteins for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... The CBP-His6-ZZ-Upf1-HD proteins were first purified on a Protino® Ni-NTA agarose resin (Macherey-Nagel). After successive washing steps using buffers C200 first ...
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bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
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bioRxiv - Molecular Biology 2019Quote: ... Twenty microliters have been collected for protein analysis and RNA was extracted from remaining supernatant with the Nucleospin RNA extraction II Kit (Macherey-Nagel).
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bioRxiv - Neuroscience 2022Quote: ... and 1:8 of His-SUMO-CREB and MBP-Jacob-45-228) were incubated with GST-LMO4 and GST (control protein) immobilized on Protino Glutathione Agarose 4B beads (Macherey-Nagel). Probes were eluted with 25µl of 2x SDS sample buffer after incubation and washing steps (washing buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of respective proteins from bacterial extracts was performed by affinity chromatography using Protino glutathione-conjugated agarose 4B (Macherey-Nagel, Düren, Germany). Except for GST-WAVE2-WCA ...
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bioRxiv - Neuroscience 2020Quote: ... The total proteins were precipitated and prepared for Polyacrylamide Gel Electrophoresis using protein precipitator and resuspension buffer PSB/TCEP (Protein solving buffer and (tris(2-carboxyethyl)phosphine) TCEP reducing agent) from the NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France). The elution fraction containing RNA was further used for PCR analysis (see below) ...