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Citations for Macherey-Nagel GmbH :
1 - 50 of 63 citations for Phosphatase Inhibitor Cocktail 2 Tubes 100X since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... elegans animals were transferred to 2 ml MN Bead Tubes Type A (Macherey-Nagel, Düren, Germany) and lysed using a Precellys Bead Beating system with an additional Cryolys cooling module (Bertin Instruments ...
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bioRxiv - Systems Biology 2024Quote: Fecal pellets (∼40 mg) were weighed in sterile ceramic bead tubes (NucleoSpin Bead Tubes; Macherey-Nagel, Dueren, Germany) and extracted with 1 mL of chilled methanol (−20 °C ...
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bioRxiv - Microbiology 2022Quote: ... Filter pieces were transferred into MN Bead Tubes Type A (Macherey-Nagel) containing 800 μl of buffer RLT Plus that were pre-chilled at −80°C for 10 minutes ...
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bioRxiv - Evolutionary Biology 2019Quote: ... attenuata was taken and incubated with PDMS tubes in a sealed 8 mL glass vial (MACHEREY-NAGEL).
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bioRxiv - Microbiology 2023Quote: ... The filters were then individually placed into 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel), added with 1,200 µL MWA1 lysis buffer and stored at - 80°C until nucleic acids extraction as specified in the corresponding section below.
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bioRxiv - Molecular Biology 2023Quote: ... Five tubes of each amplicon were pooled and DNA purified using NucleoSpin Gel and PCR Clean-Up (Macherey-Nagel). DNA was eluted in 20 µl of Elution Buffer and concentration was measured using Qubit 3.0 fluorometer (Life Technologies).
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bioRxiv - Microbiology 2023Quote: ... slight adaptations were made from manufacturer’s instructions: half MCE filters were placed into individual 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel) and added with 1,200 µL MWA1 lysis buffer ...
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bioRxiv - Plant Biology 2021Quote: ... an EC 100/2 Nucleoshell Phenyl-Hexyl column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Evolutionary Biology 2019Quote: ... one freshly opened flower was taken out of the plant at 20:00 and incubated with polydimethylsiloxane (PDMS) tubes in a sealed 8 mL glass vial (MACHEREY-NAGEL)33.
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bioRxiv - Molecular Biology 2022Quote: ... at least eight separate tubes were used for PCR and then pooled and purified by using PCR and gel extraction kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatants were transferred to fresh tubes and filtrated first through a 5 µm syringe PES filter (CHROMAFIL® Xtra PES, 5.0 µm, Macherey-Nagel), then through a 1 µm (Whatman™ Puradisc ...
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bioRxiv - Microbiology 2024Quote: ... into prefilled tube strips (8 x 12) containing 750 µl DNA/RNA ShieldTM (ZymoBIOMICS) and 40-400 µm glass beads (Macherey-Nagel) and arranged on a 96-well rack ...
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bioRxiv - Plant Biology 2021Quote: ... The aqueous phase was transferred to a new tube and incubated with 1 μL of RNase A (Macherey-Nagel, 12mg/mL stock) for 30 min at 37 °C ...
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bioRxiv - Plant Biology 2021Quote: ... The amplicons were purified from the PCR reagent tube via the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Evolutionary Biology 2022Quote: ... 35 mg of animals were transferred into a 1.5 mL tube and total RNA was extracted using a Nucleospin® RNA Plus Kit (Macherey-Nagel, Düren, Germany). The prey cDNA library was screened for interactions with the 70 distal C-terminal amino acids of the TadNaC2 and TadNaC10 channels expressed in yeast from the bait vector pGBK-T7 ...
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bioRxiv - Biochemistry 2020Quote: ... The sampling unit was pelleted on the bottom of the tube and the supernatant was transferred into HPLC vials equipped with a 200 μL V-shape glass insert (both Macherey-Nagel GmbH & Co.KG) and analysed by LC-MS/MS ...
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bioRxiv - Immunology 2020Quote: OMM bacteria were grown as described and 1 mL of each bacterial culture supernatant was homogenized using NucleoSpin Bead Tubes (Macherey-Nagel, Düren, Germany) and a Precellys Evolution Homogenizer (Bertin Corp. ...
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bioRxiv - Microbiology 2022Quote: ... Each female was held above a sterile microcentrifuge tube filled with 700 μl lysis buffer SL1 (NucleoSpin Soil kit, Macherey-Nagel, Düren, Germany), and the ovipositor was dipped into the liquid and moved in circles for one min to wash microbes off of the ovipositor’s surface (WS) ...
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bioRxiv - Plant Biology 2023Quote: ... from 2 to 3-day-old WT and mea-1-/-;dme-2-/- seedlings using NucleoSpin® Plant II (MACHEREY-NAGEL). 200 ng of DNA was subjected to fragmentation using ultrasonicator (Covaris ...
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bioRxiv - Biochemistry 2022Quote: ... Amino acids were separated on a reverse-phase C18 column (EC 250/2 NUCLEOSIL 100-3 C18HD, L=250 mm, ID: 2 mm; Macherey-Nagel). Samples (5 µL ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μL sample volume was injected into a Nucleodur HILIC column (250 mm * 2 mm, 3 μm particle size, Macherey-Nagel). Metabolites were eluted at 20 °C with an acetonitrile gradient (90% (v/v ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we measured the chemical oxygen demand (COD) using NANOCOLOR COD tube tests (detection range 1-15 g/l by Macherey-Nagel (ref: 985 038), see (32 ...
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bioRxiv - Cell Biology 2024Quote: ... RNAlater was removed then tissues were transferred into screw-capped tube pre-filled with 350 µl of RA1 buffer of NucleoSPin RNAXS kit (Macherey-Nagel Cat# SKU: 740902.250). Tissues were homogenised with 10 pcs of 3 mm Acid-Washed Zirconium Beads (OPS diagnostics Cat# BAWZ 3000-300-23 ...
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bioRxiv - Plant Biology 2020Quote: ... LMW carbonyls were separated using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an Ekspert ultraLC 100 UHPLC system (AB SCIEX ...
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bioRxiv - Plant Biology 2023Quote: ... Separation was carried out using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an ekspert ultraLC 100 UHPLC system coupled to a QTRAP 4500 mass spectrometer (AB SCIEX ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleosil 100-5 N(CH3)2 column (Macherey-Nagel GmbH & Co, Düren, Germany) and eluted isocratically with 100% methanol containing 0.01% acetic acid at a flow rate of 1 ml min-1 ...
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bioRxiv - Microbiology 2023Quote: ... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
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bioRxiv - Plant Biology 2021Quote: ... a Nucleoshell RP 18 plus reversed-phase column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Genomics 2020Quote: Total mRNA of edited HUDEP-2 cells was isolated using the NucleoSpin RNA kit (Macherey-Nagel) and reverse transcribed into cDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... The supernatant was collected and loaded onto 2 mL pre-equilibrated Ni-NTA agarose (Macherey-Nagel). The mixture was rotated at 4 □ for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... BM DOHH2 were resuspended in RA1 buffer and tris(2-carboxyethyl)phosphine (TCEP) from Macherey-Nagel and stored at -80°C for less than 3 months ...
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bioRxiv - Microbiology 2022Quote: The yeast expression vector pAG416-GAL-SARS-CoV-2-3CL was freshly prepared by Midiprep (Macherey-Nagel), and variants were introduced by a modified single primer site-directed mutagenesis method (Shenoy and Visweswariah ...
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bioRxiv - Microbiology 2023Quote: Separation was performed on an EC 100/2 Nucleoshell Phenyl-Hexyl column (2C×C100Cmm, 2.7Cμm; Macherey-Nagel) at 40°C ...
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bioRxiv - Biochemistry 2023Quote: ... SUV420H1 proteins were isolated by incubating the soluble fraction for 2 hrs with GST beads (Macherey-Nagel). The beads were washed with two column volumes of wash buffer (20 mM Tris ...
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bioRxiv - Genomics 2023Quote: ... DNA purification was carried out by adding a 2 ml mixture of wash buffer PW2 (MACHEREY-NAGEL), wash buffer B5 (MACHEREY-NAGEL) ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from isolated islets from 2 mice per genotype with Nucleospin RNA plus XS (Macherey-Nagel). Complementary DNA was generated and linearly amplified from 3 ng total RNA using the Ovation RNA-seq V2 system (NuGEN technologies Inc. ...
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bioRxiv - Genetics 2020Quote: ... Samples were separated on a C18 column (EC 100/2 Nucleoshell RP-18, 2.7 μm, from Macherey-Nagel) using a gradient of 0.1% formic acid / isopropanol over 0.1% formic acid / water ...
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bioRxiv - Molecular Biology 2023Quote: ... the soluble fraction was incubated for 2 hours in batch with Ni2+-nitrilotriacetate (NTA) agarose resin (Macherey-Nagel). After extensive washing of the beads with lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected by gel electrophoresis on 2% Agarose/TAE and gel extracted by column purification (Macherey-Nagel). Amplicons were paired-end sequenced with 500 cycles on a MiSeq system (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected for 400-550 bp using a 2% agarose/TAE gel and column purification (Macherey-Nagel). Libraries were sequenced as above ...
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bioRxiv - Genomics 2022Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA from a male wild type blood sample (2 ml) was purified with the NucleoBand®HMW DNA Kit (Macherey-Nagel). To eliminate fragments below 40 kb the Short Read Elimination Kit XL (Circulomics ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were excised from 2% agarose gels and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) using NTC buffer for solubilization of the gel slices ...
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bioRxiv - Neuroscience 2021Quote: Viral RNA was isolated from 4 µl of a typical lentivirus preparation (∼2×106 TU/µl) using the NucleoSpin RNA Virus mini kit (Macherey-Nagel). Purified RNA was used as a template for reverse transcription using the SuperScript VILO cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Solid phase extraction was carried out using Chromabond SiOH cartridges filled with 2 g of unmodified silica gel (Macherey-Nagel, Germany). Chemicals ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting bottom organic phase was dried and on-line derivatized to fatty acid methyl ester by a chloroform/methanol (1:2)-TMSH solution (Macherey-Nagel) and analyzed by gas chromatography-mass spectrometry (Agilent 5977A-7890B) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PCR products were confirmed on 2% agarose gels and purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Purified PCR products were quantified using the Quant-iT dsDNA kit (Invitrogen ...