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Citations for Macherey-Nagel GmbH :
201 - 250 of 621 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) was used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... ssDNA was column-purified using Buffer NTC (Macherey-Nagel) as a binding buffer ...
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bioRxiv - Microbiology 2019Quote: ... plus the NucleoSpin RNA/DNA Buffer Set (Macherey-Nagel) procedures ...
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bioRxiv - Immunology 2021Quote: ... or RA1 buffer supplemented with β-mercaptoethanol (Macherey-Nagel). All samples were stored at -80°C until processing ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted DNA was eluted in NE buffer (Macherey-Nagel) and quantified with dsDNA HS assay kit (Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The NaOH was neutralized by the addition of 1 M HCl and the cDNA was purified by a PCR-clean-up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Germany) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... genomic fragments containing the gRNA target site were amplified with PCR and purified using NucleoSpin Gel and a PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Then 200 ng of each of the PCR products derived from the transfected and non-transfected cells were denatured at 95° C for 5 min and reannealed ...
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bioRxiv - Plant Biology 2021Quote: ... The amplicons were purified from the PCR reagent tube via the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Genomics 2021Quote: ... Gel extraction is performed to isolate the PCR product with the correct size range using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). PacBio sequencing library preparation for both libraries was done by the Genomics Core Leuven (KU Leuven) ...
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bioRxiv - Microbiology 2023Quote: ... Read length was assessed by DNA agarose gel electrophoresis (1% w/v) and amplicons were purified from the PCR mix using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, DE) following manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... PCR amplicons were purified via gel purification or column purification using the NucleoSpin Gel and PCR Clean–up kit (Macherey-Nagel, Cat.#740609.50). Amplicon sequences were verified by Sanger sequencing using the Overnight Mix2Seq Kit service (Eurofins Genomics) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we purified the amplification reaction following the PCR clean-up protocol of the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Finally ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products from genomic DNA and cDNA were purified using NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Allentown, PA, USA), and quality was validated via agarose gel electrophoresis.
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bioRxiv - Neuroscience 2020Quote: ... The total proteins were precipitated and prepared for Polyacrylamide Gel Electrophoresis using protein precipitator and resuspension buffer PSB/TCEP (Protein solving buffer and (tris(2-carboxyethyl)phosphine) TCEP reducing agent) from the NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France). The elution fraction containing RNA was further used for PCR analysis (see below) ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR products were checked out in an agarose gel and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Finally ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR fragments were extracted from agarose gels (Macherey-Nagel, 740609.250). pBlueRabbit vector was digested with EagI and XbaI ...
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bioRxiv - Immunology 2019Quote: ... using NucleoSpin Gel and PCR Clean-up kits (Macherey-Nagel), and the DNA was then used for PCR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or by NucleoSpin Gel and PCR clean-up (Macherey-Nagel). PCR products were sequenced by Genewiz ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were purified using NucleoSpin® kit (Macherey-Nagel). Plasmids generated ...
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bioRxiv - Bioengineering 2022Quote: ... NucleoSpin Gel and PCR Clean-up Midi kit (MACHEREY-NAGEL) were used to extract the pooled PCR products from 24 samples in 200 μl of water ...
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bioRxiv - Microbiology 2020Quote: ... gel purified (Nucleoscopic gel and PCR cleanup kit; Macherey-Nagel), quality checked (BioAnalyzer HS DNA kit ...
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bioRxiv - Immunology 2019Quote: ... purified using a PCR purification kit (Macherey-Nagel Nucleospin; 740609), and sent for Illumina sequencing ...
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bioRxiv - Bioengineering 2021Quote: ... or NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). gRNA targeting BFP (gBFP ...
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bioRxiv - Immunology 2022Quote: ... PCR products were first purified using NucleoSpin kit (Macherey-Nagel) followed by Ampure bead purification (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified using the “PCR Clean-up System” (Macherey-Nagel).
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bioRxiv - Neuroscience 2022Quote: ... concentrated using a PCR cleanup kit (Macherey-Nagel, Düren, Germany), and run on the Illumina NextSeq 500 sequencing platform (Illumina Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... using the PCR and gel clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... NucleoSpin Gel and PCR Clean-up Kits (Macherey-Nagel, Germany) were used for DNA clean-up from PCR products and agarose gel extracts ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was purified using the PCR Purification kit (Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... and followed with a PCR clean-up (Macherey-Nagel, F40609.250). PCR products and plasmids were restricted with FastDigest BshTI (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), and cloned into pGEM-T Easy Vector (Promega) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The PCR products were purified using NucleoSpin columns (Macherey-Nagel) and eluted into 33 μL water ...
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bioRxiv - Developmental Biology 2022Quote: ... Dissected tissue was immediately placed into lysis buffer (Macherey-Nagel) on dry ice and stored at -80C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The DNA pellet was digested in CF buffer (Macherey-Nagel) for 45 min at 65°C and purified using NucleoBond AXG20 Mini columns according to the user manual (Macherey-Nagel) ...
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bioRxiv - Biophysics 2022Quote: ... and the 3’-terminal poly A) were amplified by PCR and purified using the NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... containing 1819 adenine bases) was amplified by PCR and purified using a NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) with Milli-Q water ...
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bioRxiv - Genetics 2021Quote: ... followed by purification (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Membranes were washed twice with 2x SSC ...
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bioRxiv - Bioengineering 2020Quote: ... NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Germany) was used for DNA clean-up both from PCR products and agarose gel extracts ...
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bioRxiv - Cell Biology 2021Quote: ... The product was purified by NucleoSpin PCR clean-up (Macherey-Nagel) and transformed into Escherichia coli ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted using Nucleospin PCR clean-up kit (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl pH 8.5) ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted using a Nucleospin PCR cleanup kit (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl [pH 8.5]) ...
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bioRxiv - Immunology 2022Quote: ... and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel).
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bioRxiv - Neuroscience 2019Quote: ... purified with Nucleospin Gel and PCR Clean-up (Macherey-Nagel, Germany), and cloned into pEX-A (Eurofins Genomics ...
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bioRxiv - Microbiology 2021Quote: ... The NucleoSpin® Gel and PCR Clean-up (MACHEREY-NAGEL, Germany) were used to purify the PCR products ...
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bioRxiv - Microbiology 2020Quote: ... and purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel). Then ...
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bioRxiv - Microbiology 2021Quote: ... using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Gel-purified amplicons were then assembled using NEBuilder HiFi DNA Assembly Master Mix at a 1:1 molar ratio for each DNA fragment and incubated at 50°C for two hours ...
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bioRxiv - Plant Biology 2022Quote: ... purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and circularized using T4 DNA ligase.
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bioRxiv - Synthetic Biology 2022Quote: A NucleoSpin PCR clean-up gel extraction kit (Macherey-Nagel, Germany) was used to purify PCR products before their use in cloning ...
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bioRxiv - Microbiology 2022Quote: ... NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Bethlehem, PA) was used to remove contaminants ...
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bioRxiv - Molecular Biology 2023Quote: ... or the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s instructions ...