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Citations for Macherey-Nagel GmbH :
51 - 100 of 224 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was separated from the debris by centrifugation at 4000g for 40 minutes at 4°C and the supernatant was purified using 1 ml Ni-NTA columns (Macherey-Nagel). His-tagged UL12 was eluted using lysis buffer with increasing concentration of Imidazole (100 mM ...
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bioRxiv - Developmental Biology 2024Quote: ... The His6-3C protease was then removed from the sample by immobilized metal affinity chromatography using a 1 mL Protino Ni-NTA column (Macherey-Nagel). The mTRIM66 PHD-bromo-domain protein was further purified by size exclusion chromatography using a HiLoad 16/600 Superdex 75 pg column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μL sample volume was injected into a Nucleodur HILIC column (250 mm * 2 mm, 3 μm particle size, Macherey-Nagel). Metabolites were eluted at 20 °C with an acetonitrile gradient (90% (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... The aqueous phase was transferred to a new tube and incubated with 1 μL of RNase A (Macherey-Nagel, 12mg/mL stock) for 30 min at 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... LMW carbonyls were separated using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an Ekspert ultraLC 100 UHPLC system (AB SCIEX ...
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bioRxiv - Plant Biology 2023Quote: ... Separation was carried out using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an ekspert ultraLC 100 UHPLC system coupled to a QTRAP 4500 mass spectrometer (AB SCIEX ...
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bioRxiv - Immunology 2020Quote: OMM bacteria were grown as described and 1 mL of each bacterial culture supernatant was homogenized using NucleoSpin Bead Tubes (Macherey-Nagel, Düren, Germany) and a Precellys Evolution Homogenizer (Bertin Corp. ...
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bioRxiv - Molecular Biology 2020Quote: ... 1mg/mL proteinase K (Macherey-Nagel) and then with 0.1 mg/mL RNaseA (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... then added 1.2 µL of RNase (MN, Macherey-Nagel, Ref. 740505, 1200 U/mL, 10 mg/mL), and transferred the DNA to a 96-well KingFisher Flex plate (ThermoFischer Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were resuspended in PBS with 20 µg/ml PI and 0.2 mg/mL RNAse A (Macherey-Nagel) and incubated at RT for 30 min before analysis on a FACSCalibur (Beckton Dickinson) ...
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bioRxiv - Biochemistry 2021Quote: ... with an EC 250/4 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel) with a general gradient of 10% to 90% ACN in H2O ...
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bioRxiv - Microbiology 2019Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Macherey-Nagel #740579) and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... placed in 100 μl of T1 extraction buffer (Nucleo-Spin Tissue, Macherey-Nagel), and stored at −80°C until DNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Macherey-Nagel #740579) and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075).
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bioRxiv - Microbiology 2022Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Macherey-Nagel #740579) and were transfected into early passage NIH-3T3 cells in 6 well plates using TransIT-X2® dynamic delivery transfection system (Mirus) ...
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bioRxiv - Biochemistry 2022Quote: ... VP Nucleodur 100-5C 18 ec column (250 ×40 mm, 7 μm: Macherey-Nagel) used as stationary phase ...
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bioRxiv - Biochemistry 2021Quote: ... with a VP 250/10 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel, Düren, Germany) running a general gradient of 10% to 70% acetonitrile (ACN ...
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bioRxiv - Plant Biology 2022Quote: ... with the difference that a Nucleodur 100-5 NH2 125x4 mm column (Macherey-Nagel, #760730.40) and an eluent consisting of 8.5% acetonitrile and 25mM formic acid pH 4 were used (18) ...
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bioRxiv - Biochemistry 2021Quote: ... The Ni2+-NTA column (4 ml; Macherey-Nagel, Düren) was equilibrated with ten column volumes of the solubilization buffer before loading the sample ...
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bioRxiv - Developmental Biology 2023Quote: ... permeabilized with 10 µg/mL Proteinase K (Macherey-Nagel) in PBS containing 0.1% tween (PBST ...
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 250 μg/mL of RNase A (Macherey-Nagel) and 0.5 mg/mL of lysing enzymes from Trichoderma harzianum (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5% β-mercaptoethanol and 10 μg/mL RNaseA (Macherey-Nagel)) and incubated for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5% β-mercaptoethanol and 10 μg/mL RNaseA (Macherey-Nagel)) and incubated for 1 hr at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 7.5% β-mercaptoethanol and 10 μg/ml RNaseA (Macherey-Nagel). The plugs were then incubated overnight at 50°C in a solution containing 250 mM EDTA (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from 100 μL blood using the Genomic DNA Extraction kit (Macherey-Nagel, Düren, Germany) and eluted in an equivalent volume of elution buffer ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... The concentrate was applied to a C18 column (Polygoprep C18, 25–40 μm, 100 Å (Macherey-Nagel), 40 i.d ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using 100 µL of homogenate with the Genomic DNA tissue kit (Macherey-Nagel, Germany). Detection and quantification of R ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μg/ml proteinase K (PK, Macherey-Nagel, Düren, Germany, 740506)) ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were permeabilized with 10 μg/ml Proteinase K (Macherey-Nagel) in PBS containing 0.1% tween (PBST ...
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bioRxiv - Bioengineering 2022Quote: ... 20 µL of proteinase K (20 mg/mL; MACHEREY-NAGEL # 740506) was added and the mixture incubated at 70°C for 20 min ...
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bioRxiv - Plant Biology 2021Quote: ... dissolved and fractionated by RP-HPLC using a Nucleodur 100-5 C18ec 125×4.6 mm column (Macherey-Nagel). The ABA-containing fraction was collected ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of homogenate was set aside as total cell (T) and mixed with NucleoZOL (Macherey-Nagel, #740404.200) before storage at -80°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... preparative HPLC was employed using a reversed-phase C18 column (Macherey-Nagel VP 250/21 Nucleodur 100-5) with a mobile phase solvent A (Water + 0.05% vol/vol trifluoroacetic acid (TFA)) ...
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bioRxiv - Genomics 2020Quote: Total mRNA of edited HUDEP-2 cells was isolated using the NucleoSpin RNA kit (Macherey-Nagel) and reverse transcribed into cDNA ...
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bioRxiv - Cancer Biology 2023Quote: ... BM DOHH2 were resuspended in RA1 buffer and tris(2-carboxyethyl)phosphine (TCEP) from Macherey-Nagel and stored at -80°C for less than 3 months ...
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bioRxiv - Plant Biology 2021Quote: ... Separation of metabolites was achieved on a Nucleoshell RP18 column (100 x 4.6 mm, 2.7 µm; Macherey-Nagel, Germany). The column was equipped with a precolumn (Macherey–Nagel ...
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bioRxiv - Cell Biology 2021Quote: ... The amplified rDNA gene fragments were purified using nucleobond AX 100 columns for Miniprep System (Macherey-Nagel, ref 740521.100). The rDNA sequences were then labeled with Digoxigenin-11-dUTP by nick translation according to the manufacturer’s protocol (Roche ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and 100 µL of the supernatant was collected in gaschromatography (GC) glass vials (Macherey-Nagel, Düren, Germany); both were stored at -80°C ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from approximately 100 mg of ground tissue using the NucleoSpin RNA Plant Kit (Macherey-Nagel) and treated with DNase I on the column ...
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bioRxiv - Microbiology 2022Quote: ... sodium deoxycholate 1.5%) containing 200 μg/mL of RNAse A (Macherey-Nagel), and incubated during 15 min at 37 °C ...
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bioRxiv - Microbiology 2022Quote: The yeast expression vector pAG416-GAL-SARS-CoV-2-3CL was freshly prepared by Midiprep (Macherey-Nagel), and variants were introduced by a modified single primer site-directed mutagenesis method (Shenoy and Visweswariah ...
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bioRxiv - Biochemistry 2023Quote: ... SUV420H1 proteins were isolated by incubating the soluble fraction for 2 hrs with GST beads (Macherey-Nagel). The beads were washed with two column volumes of wash buffer (20 mM Tris ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from 100 mg of ripe fruits using the Nucleo-spin RNA Plant mini kit (Macherey-Nagel). Library preparation and Nanopore sequencing were performed at the Ecole normale superieure genomic core facility (Paris ...
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bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was prepared from 100 mg of 11-day-old seedlings with the NucleoSpin RNA Plant Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Then total RNAs were extracted from 100 µL of homogenates using the Nucleospin RNA II extract kit (Macherey-Nagel, Germany) and 200 µL were conserved at −80°C for attempts to isolate the virus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The drug concentration in samples was measured with high-performance liquid chromatography (HPLC) using a reverse phase C18 Nucleosil 100-5 (4.0 × 250 mm; 5.0 μm particle size, Macherey-Nagel, Germany) column and mobile phase consisting of MeOH/water (90/10 v/v ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from isolated islets from 2 mice per genotype with Nucleospin RNA plus XS (Macherey-Nagel). Complementary DNA was generated and linearly amplified from 3 ng total RNA using the Ovation RNA-seq V2 system (NuGEN technologies Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... the soluble fraction was incubated for 2 hours in batch with Ni2+-nitrilotriacetate (NTA) agarose resin (Macherey-Nagel). After extensive washing of the beads with lysis buffer ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from ~100 mg of feces with the use of a Nucleospin DNA Stool Kit (740472, Macherey-Nagel) according to the manufacturer’s instructions with the following modifications ...