Labshake search
Citations for Macherey-Nagel GmbH :
1 - 50 of 84 citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
-
bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
-
bioRxiv - Neuroscience 2019Quote: ... Total protein was isolated using an RNA-protein extraction kit (Macherey-Nagel), as specified by the manufacturer ...
-
bioRxiv - Genetics 2022Quote: ... The protein concentration was determined by Protein Quantification Assay (Cat.# 740967,Macherey-Nagel). 10 µg of the protein was separated by SDS-PAGE electrophoresis ...
-
bioRxiv - Molecular Biology 2020Quote: ... The protein concentration was quantified using the Protein Quantification Assay kit (Macherey-Nagel). The protein samples were resolved using 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gels (Bio-Rad ...
-
bioRxiv - Immunology 2021Quote: ... All protein concentrations were determined with the MN Protein Quantification Assay (Macherey-Nagel). Western blotting was performed utilizing AnykD Mini-PROTEAN TGX Gels (Biorad ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein samples were extracted from the organs with the NucleoSpin RNA/Protein kit (Macherey-Nagel). Macherey-Nagel Bead Tubes Type F was used in tissue homogenization ...
-
bioRxiv - Cell Biology 2023Quote: ... and protein isolation kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... and protein extraction kit (Macherey-Nagel). RNA was further purified by using RNA Clean & Concentrator-25 kit (Zymo Research ...
-
bioRxiv - Genomics 2024Quote: ... RNA & Protein Purification kit (Macherey-Nagel). Clones were screened by XhoI digestion and Sanger sequencing.
-
bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
-
bioRxiv - Microbiology 2023Quote: ... a 450 bp long sequence upstream of timA was synthesized by PCR from EGD-e genomic DNA using primers TE74 and TE75 and purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). For the EMSA experiment ...
-
bioRxiv - Genomics 2020Quote: Total protein was extracted from differentiated neurons at DIV 35 with the NucleoSpin RNA/Protein Kit (Macherey-Nagel 740933) according to manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: RNA and proteins (used for the proteomic study) were extracted using the NucleoSpin® RNA/Protein extraction kit (Macherey-Nagel). RNA quantification was performed using a Nanodrop® spectrophotometer (ND-1000) ...
-
bioRxiv - Developmental Biology 2023Quote: ... and protein extraction kit (Macherey-Nagel, Allentown, PA) (gene expression studies ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein and mRNA were extracted from the same leaf sample (NucleoSpin RNA/Protein kit, REF740933, Macherey-Nagel GmbH & Co., Düren, Germany).
-
bioRxiv - Biochemistry 2021Quote: ... Cleaved protein was passed through Ni-TED (Macherey-Nagel) to remove uncleaved protein ...
-
bioRxiv - Biochemistry 2023Quote: All proteins were purified using Ni-IDA (Macherey-Nagel) following the instructions provided by the manufacturer ...
-
bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
-
bioRxiv - Immunology 2021Quote: ... total protein was isolated by NucleoSpin TriPrep kit (Macherey-Nagel) from 5×106 PMNs ...
-
bioRxiv - Plant Biology 2022Quote: ... Proteins were batch-purified using Ni-NTA agarose resin (Macherey-Nagel) and eluted using the abovementioned lysis buffer with increased imidazole concentration (250 mM) ...
-
bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified using Protino NTA columns (Macherey-Nagel, Freemansburg, PA, USA) using linear imidazole gradients (30-250 mM) ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were purified at 4 °C on Ni-NTA-Sepharose (Macherey-Nagel) packed in a gravity flow column ...
-
bioRxiv - Immunology 2022Quote: ... The protein was purified on a Ni-NTA resin column (Macherey-Nagel) and the eluted fractions were concentrated and dialyzed against PBS using the same Amicon system as described above ...
-
bioRxiv - Cell Biology 2019Quote: mRNA was isolated using the NucleoSpin® RNA/Protein kit (Macherey-Nagel). Purified mRNA was transcribed into cDNA with the QuantiTect®Reverse Transcription Kit (Qiagen) ...
-
bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
-
bioRxiv - Cell Biology 2023Quote: ... The proteins were then transferred onto Porablot NCP nitrocellulose membrane (Macherey-Nagel) and blocked for 1 hour at RT in 3% BSA (Applichem ...
-
bioRxiv - Biochemistry 2023Quote: ... The GST fusion proteins were purified with Protino Glutathione Agarose 4B (Macherey-Nagel) and GST was cleaved by human thrombin (Biopharm laboratories ...
-
bioRxiv - Plant Biology 2024Quote: ... The protein was purified using Protino Ni-NTA agarose (Macherey-Nagel, #7450400-500).
-
bioRxiv - Biochemistry 2019Quote: ... Proteins were captured using glutathione-agarose column (Protino glutathione-agarose 4B, Macherey-Nagel Gmbh) equilibrated with 20 mM Tris HCl ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein and RNA extractions were performed with NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... The Psyn ThyX recombinant protein was purified on Protino Ni-TED column (Macherey-Nagel) as previously described49 ...
-
bioRxiv - Immunology 2019Quote: Total RNA was extracted using the total RNA and protein isolation kit (Macherey-Nagel). RNA samples were retrotranscribed (High-Capacity cDNA Reverse Transcription kit ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... NuleoSpin® RNA/Protein extraction kit (740933.50) was purchased from Macherey-Nagel (Hoerdt, France). Sequencing grade modified trypsin (V5111) ...
-
bioRxiv - Microbiology 2020Quote: ... Protein was purified by SPE with a Chromabond SPE-C4 cartridge (Macherey-Nagel, Germany). Briefly ...
-
bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted with the Nucleospin RNA/Protein kit (Macherey-Nagel, Düren, DE) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
-
bioRxiv - Biophysics 2022Quote: ... and the protein was affinity- purified from lysate using Ni-NTA affinity resin (Macherey-Nagel) and elution with imidazole ...
-
bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from primary mouse hepatocytes using NucleoSpin kit (Macherey-Nagel cat# 740955.25) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... The proteins were purified from the cleared lysate with Ni-NTA agarose (Macherey-Nagel, Düren, Germany) following concentration and dialysis against 0.1 M potassium phosphate buffer pH 7.
-
bioRxiv - Neuroscience 2022Quote: RNA used for sequencing was extracted from tissue using the NucleoSpin RNA/Protein Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... GST-tagged proteins in soluble extracts were purified by GSH-agarose beads (Macherey-Nagel, Düren, Germany) from which were eluted by the addition of 50 mM reduced L-glutathione (dissolved in 25 mM Tris-HCl pH 8).
-
bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were then purified from bacterial extracts by affinity chromatography using glutathione-conjugated agarose (Macherey-Nagel), followed by cleavage of the GST-tag with PreScission protease ...
-
bioRxiv - Systems Biology 2022Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
-
bioRxiv - Immunology 2022Quote: Total RNA from cultured cells was extracted using the Nucleospin RNA/Protein kit (Macherey-Nagel, Düren, Germany). RNA was retrotranscribed using SuperScript III reverse transcriptase (Invitrogen ...
-
bioRxiv - Systems Biology 2022Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
-
bioRxiv - Biochemistry 2023Quote: ... SUV420H1 proteins were isolated by incubating the soluble fraction for 2 hrs with GST beads (Macherey-Nagel). The beads were washed with two column volumes of wash buffer (20 mM Tris ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein samples resolved in agarose gel were vacuum-transferred onto the nitrocellulose membrain (Porablot, MACHEREY-NAGEL), and subjected to a standard Western-Blotting procedure with either anti-Sup35NM ...