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Citations for Macherey-Nagel GmbH :
1 - 50 of 165 citations for Dickkopf related protein 1 DKK 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
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bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
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bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
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bioRxiv - Biochemistry 2021Quote: ... total RNA from HEK293 cells were isolated using the NucleoSpin RNA kit (Macherey-Nagel, Bethlehem, PA). cDNA was synthesized using the iScript kit (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... total RNAs were isolated from HeLa and HEK293 cells using the NucleoSpin RNA kit (Macherey-Nagel). To examine endogenous mRNA expression levels and the splicing products ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the DNA of HEK293 cells was extracted and purified following the NucleoSpin Tissue protocol (Macherey-Nagel, Düren, Germany). The genotype at rs1047626 was verified to be heterozygote (AG ...
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bioRxiv - Microbiology 2023Quote: ... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
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bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... Total protein was isolated using an RNA-protein extraction kit (Macherey-Nagel), as specified by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:100 RNase A (MACHEREY-NAGEL, 740505) was added in each sample for 15 min at room temperature ...
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bioRxiv - Genetics 2022Quote: ... The protein concentration was determined by Protein Quantification Assay (Cat.# 740967,Macherey-Nagel). 10 µg of the protein was separated by SDS-PAGE electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration was quantified using the Protein Quantification Assay kit (Macherey-Nagel). The protein samples were resolved using 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gels (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... All protein concentrations were determined with the MN Protein Quantification Assay (Macherey-Nagel). Western blotting was performed utilizing AnykD Mini-PROTEAN TGX Gels (Biorad ...
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bioRxiv - Microbiology 2020Quote: ... The lysed culture was centrifuged at 46,000 x g for 1 hour and the supernatant was loaded onto a 1-ml Protino® Ni-NTA column (Macherey-Nagel). The protein was eluted with buffer A + 300 mM imidazole and protein-containing fractions were pooled and dialyzed overnight in buffer A with TEV protease (1/10 w/w ratio ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 100 μL of 1:1 diluted apical wash using the NucleoMag VET (Macherey-Nagel AG, Oensingen, Switzerland), according to the manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein samples were extracted from the organs with the NucleoSpin RNA/Protein kit (Macherey-Nagel). Macherey-Nagel Bead Tubes Type F was used in tissue homogenization ...
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The complete mitochondrial genome of Spurilla braziliana MacFarland 1909 (Nudibranchia, Aeolidiidae)bioRxiv - Genomics 2023Quote: ... Japan (Figure 1) and preserved in NucleoProtect RNA (MACHEREY-NAGEL) for 5.5 months at -80 °C before DNA extraction ...
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bioRxiv - Cell Biology 2023Quote: ... and protein isolation kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and protein extraction kit (Macherey-Nagel). RNA was further purified by using RNA Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Genomics 2024Quote: ... RNA & Protein Purification kit (Macherey-Nagel). Clones were screened by XhoI digestion and Sanger sequencing.
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bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
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bioRxiv - Physiology 2022Quote: ... Human keratinocyte RNA was purified using NucleoSpin RNA (Macherey-Nagel, Düren, Germany). Before the RNA preparation ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was loaded onto 1 ml Ni-NTA agarose beads (Macherey-Nagel), pre-equilibrated with 10 ml wash buffer and rotated end-over-end for 1h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 mM NaCl and 1 mg/ml proteinase K (Macherey-Nagel, 740506) and 0.05 mg/ml RNase A (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: Total protein was extracted from differentiated neurons at DIV 35 with the NucleoSpin RNA/Protein Kit (Macherey-Nagel 740933) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA from human islets was extracted using Nucleospin RNA XS (Macherey-Nagel) three days post-transfection.
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA and proteins (used for the proteomic study) were extracted using the NucleoSpin® RNA/Protein extraction kit (Macherey-Nagel). RNA quantification was performed using a Nanodrop® spectrophotometer (ND-1000) ...
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bioRxiv - Developmental Biology 2023Quote: ... and protein extraction kit (Macherey-Nagel, Allentown, PA) (gene expression studies ...
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bioRxiv - Genetics 2019Quote: ... The frozen pellet was resuspended in 1 ml of RA1 buffer (Macherey-Nagel) supplemented 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Genomics 2023Quote: ... The ground tissues were lysed with 1 ml CF lysis buffer (MACHEREY-NAGEL) supplemented with 20 μl RNase and incubated for 1 h at 65°C under agitation ...
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bioRxiv - Plant Biology 2023Quote: ... Protein and mRNA were extracted from the same leaf sample (NucleoSpin RNA/Protein kit, REF740933, Macherey-Nagel GmbH & Co., Düren, Germany).
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Solid-phase extraction cartridges (Chromabond HR-X, 30mg, 1 ml, Macherey-Nagel, Dueren, Germany) were conditioned with 1 ml MeOH followed by 1 ml distilled HPLC-water ...
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bioRxiv - Plant Biology 2020Quote: ... The pellet was resuspended on ice in 1 ml NucleoZol (Macherey-Nagel, Düren, Germany) and incubated for 10 minutes at 65°C with shaking at 250 rpm ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from 1 × 106 cells using NucleoSpin DNA RapidLyse (MACHEREY-NAGEL). qPCR was performed with primers targeting the ND2 locus in the mitochondrial genome (5′-TGTTGGTTATACCCTTCCCGTACTA-3′ and 5′- CCTGCAAAGATGGTAGAGTAGATGA-3′ ...
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bioRxiv - Microbiology 2023Quote: ... 29-18-1 using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved protein was passed through Ni-TED (Macherey-Nagel) to remove uncleaved protein ...
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bioRxiv - Biochemistry 2023Quote: All proteins were purified using Ni-IDA (Macherey-Nagel) following the instructions provided by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... −20M and GLT1cc infected human liver chimeric mice using the NucleoSpin Virus kit (Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from 1 mL overnight culture using NucleoSpin Plasmid Easypure columns (Macherey-Nagel) from the Plasmid Easypure Kit (Nucleospin).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with preequilibrated Protino Ni-NTA beads (1 ml resin, Macherey-Nagel) at 4°C for 1h ...
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bioRxiv - Microbiology 2023Quote: ... PA14 and PAK from ∼1 × 108 CFU using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel), as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... total protein was isolated by NucleoSpin TriPrep kit (Macherey-Nagel) from 5×106 PMNs ...
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jouvence, a new human H/ACA snoRNA involves in the control of cell proliferation and differentiationbioRxiv - Genetics 2020Quote: Total RNA from human cell lines were extracted using NucleoSpin RNA Plus columns (Macherey-Nagel, France), according to the manufacturer’s instructions ...