Labshake search
Citations for Macherey-Nagel GmbH :
51 - 100 of 104 citations for Dengue Virus serotype 4 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Clonal virus populations were characterized by isolating viral RNA from 150 µl aliquots of the cell culture supernatant with the NucleoSpin® RNA Virus kit (MACHEREY-NAGEL) followed by conventional RT-PCR and bidirectional Sanger sequence analysis.
-
bioRxiv - Zoology 2024Quote: ... RNA from 150 µl of excreta solution was extracted by adding 600 µl of RAV1 lysis buffer and using NucleoSpin virus RNA kit (Macherey-Nagel, France).
-
bioRxiv - Genetics 2019Quote: ... Total RNA was extracted from the lysate using NucleoSpin RNA Set for NucleoZOL (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: Total RNAs were extracted from cell-lysates using the NucleoSpin RNA II Kit (Macherey-Nagel) following the manufacturer’s protocol and were eluted in nuclease-free water ...
-
bioRxiv - Microbiology 2021Quote: Total RNAs were extracted from cell lysates using the NucleoSpin RNA II Kit (Macherey-Nagel) following the manufacturer’s protocol and were eluted in nuclease-free water ...
-
bioRxiv - Biophysics 2022Quote: ... and the protein was affinity- purified from lysate using Ni-NTA affinity resin (Macherey-Nagel) and elution with imidazole ...
-
bioRxiv - Microbiology 2024Quote: Total RNA from cell lysates was isolated with a NucleoSpin RNA purification kit (Macherey-Nagel). Purified whole cell RNA was converted to cDNA using the high-capacity cDNA reverse transcription kit (Applied Biosystems) ...
-
bioRxiv - Microbiology 2023Quote: Total RNAs from cell lysates were extracted using the NucleoSpin RNA II kit (Macherey-Nagel) following the manufacturer’s protocol and were eluted in nuclease-free water ...
-
bioRxiv - Molecular Biology 2024Quote: Total RNA form cell lysates was obtained using a NucleoSpin® RNA kit (Macherey-Nagel), according to the manufacturer’s instructions and with on-column DNAse treatment ...
-
bioRxiv - Developmental Biology 2024Quote: ... the cleared lysate was loaded onto a 5 mL Protino GST/4B column (Macherey-Nagel) and washed with 10 column volumes of lysis buffer ...
-
bioRxiv - Epidemiology 2020Quote: ... 20 µL of Proteinase K were added to 180 µL of crushed tick sample and DNA was extracted using the NucleoSpin® 96 virus Core kit (Macherey-Nagel, Germany) and the automatic platform Biomek4000 (Beckman Coulter) ...
-
bioRxiv - Microbiology 2021Quote: ... total RNA was extracted from all cell lysates using the NucleoSpin RNA II kit (Macherey-Nagel).
-
bioRxiv - Biochemistry 2022Quote: ... The proteins were purified from the cleared lysate with Ni-NTA agarose (Macherey-Nagel, Düren, Germany) following concentration and dialysis against 0.1 M potassium phosphate buffer pH 7.
-
bioRxiv - Immunology 2023Quote: Total RNA from cell lysates was extracted using the NucleoSpin RNA II kit (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from the lysates using the NucleoMag Vet kit (Macherey-Nagel, cat.no. 744200) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation and ZAP-S was captured using Ni-NTA resin (Macherey-Nagel). After elution with 500 mM imidazole ...
-
bioRxiv - Biochemistry 2022Quote: ... and viral RNA from cell lysate was extracted using the Nucleospin RNA plus mini kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... 4°C and the supernatant was incubated at 4 °C with 1.25 ml Ni-NTA agarose resin (Macherey-Nagel) for 1 h with agitation ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from cell-free lysate by metal ion affinity chromatography with Protino Ni-NTA Agarose (Macherey-Nagel) as matrix followed by ultrafiltration (Amicon Ultra-15 centrifugal filter units ...
-
bioRxiv - Cell Biology 2019Quote: ... lysates prepared from infected and stimulated cells were collected and RNA extracted using a NucleoSpin RNA kit (Macherey-Nagel, 740955). The yield and purity of the extracted RNA was assessed using a spectrophotometer (DS-11 FX ...
-
bioRxiv - Cell Biology 2022Quote: ... lysates were centrifuged at 21000 g for 10 min and supernatants were transferred to silica membrane spin columns (Macherey-Nagel) and centrifuged at 11000 g for 1 min ...
-
bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Molecular Biology 2021Quote: ... His6-BsrA was purified from the cell lysate by chromatography on Ni-agarose columns (Protino Ni-TED 1000, Macherey-Nagel) with 300 mM imidazole in phosphate buffer used for elution ...
-
bioRxiv - Biochemistry 2024Quote: ... Clarified lysates were filtered (0.45 µm syringe tip filters) and applied to pre-equilibrated Protino Ni-NTA Agarose (Macherey-Nagel) beats in a gravety column ...
-
bioRxiv - Biochemistry 2021Quote: ... The Ni2+-NTA column (4 ml; Macherey-Nagel, Düren) was equilibrated with ten column volumes of the solubilization buffer before loading the sample ...
-
bioRxiv - Genetics 2021Quote: ... The lysates were then purified to genomic DNA and RNA using NucleoSpin Tissue Kits and NucleoSpin RNA Plus kits (MACHEREY-NAGEL), respectively ...
-
bioRxiv - Bioengineering 2022Quote: ... The remaining cuticular wax-chloroform mixture was filtered (cellulose folded filter paper; 4-12 µm pore size, M&N 615 1/4, Ø 185 mm, Macherey-Nagel GmbH & Co. KG), and chloroform was evaporated (rotary evaporator ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were purified at 4 °C on Ni-NTA-Sepharose (Macherey-Nagel) packed in a gravity flow column ...
-
bioRxiv - Biochemistry 2021Quote: ... with an EC 250/4 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel) with a general gradient of 10% to 90% ACN in H2O ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were either fixed with 4% paraformaldehyde or lysed with NucleoZOL (MACHEREY-NAGEL) 17 days post infection ...
-
bioRxiv - Microbiology 2023Quote: ... the cooled mixture was filtered using a Whatman filter (no. 4, Macherey-Nagel, Germany). The off-white solid (non-PHBV cell mass ...
-
bioRxiv - Microbiology 2024Quote: ... PO4 concentrations were analysed after filtering extraction solution (Mn 619 G1/4, Macherey-Nagel) using continuous flow analyser (Seal Analytical ...
-
bioRxiv - Plant Biology 2022Quote: Total RNAs were extracted from 4-week-old adult plants using NucleoSpin RNA Plant (MACHEREY-NAGEL) kit ...
-
bioRxiv - Biochemistry 2022Quote: ... 4°C) and fragments from the supernatant purified with a Nucleospin PCR clean-up kit (Macherey-Nagel). Illumina sequencing libraries were prepared using the Hyperprep kit (KAPA ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4 °C) and fragments from the supernatant purified with a Nucleospin PCR clean-up kit (Macherey-Nagel). Experimental success was evaluated by capillary electrophoresis (Agilent ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4 °C) and fragments from the supernatant were purified by PCR clean-up spin column (Macherey-Nagel).
-
bioRxiv - Cell Biology 2020Quote: ... All plasmids were purified from 4 ml bacterial culture using either NucleoSpin® Plasmid (REF 740588.50, Macherey-Nagel®) or PureLink™ HiPure Plasmid Miniprep Kit (Art ...
-
bioRxiv - Microbiology 2022Quote: ... 4°C) and 100 µL of the supernatant was collected in gaschromatography (GC) glass vials (Macherey-Nagel, Düren, Germany); both were stored at -80°C ...
-
bioRxiv - Bioengineering 2022Quote: ... Germany) with a guard column (EC 4/3 NUCLEODUR C18 Gravity, 3 μm, Macherey-Nagel GmbH & Co. KG Germany), while the column temperature was kept at 40 °C in a thermostatic column compartment (Thermo Fisher UltiMate 3000) ...
-
bioRxiv - Immunology 2021Quote: ... Virus was purified by centrifugation at 12,000 xg for 10 mins at 4 °C and genomic DNA extracted (NucleoSpin Tissue kit, Macherey-Nagel). Next generation sequencing (NGS ...
-
bioRxiv - Biophysics 2019Quote: ... centrifuged (60,000xg, 45min at 4°C) and the supernatant was incubated with Nickel agarose beads (Protino Ni-TED, Macherey-Nagel) for 1h at 4°C.
-
bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected onto an RP18 column (4 × 50 mm, 2.7 μm particle size, Nucleoshell, Macherey-Nagel) with a flow rate of 0.25 mL min−1 ...
-
bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from the spleen of 4-6 weeks-old asymptomatic mice using nucleospin RNA II (Macherey-Nagel). The quality of RNA was checked with Bioanalyzer Agilent 2100 and RNAs with a RIN (RNA integrity number ...
-
bioRxiv - Plant Biology 2024Quote: ... Samples (80 µL) were separated on a NUCLEOSIL® C18 column (EC 250/4, 300-5, MACHEREY-NAGEL GmbH, Germany) in a LaChrome Elite HPLC equipped with an L-2130 pump module ...
-
bioRxiv - Systems Biology 2021Quote: ... the amplified plasmid library was extracted from bacterial cells using 4 reactions of Midiprep kit (Macherey-Nagel NucleoBond Xtra Midi Plus).
-
bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was separated from the debris by centrifugation at 4000g for 40 minutes at 4°C and the supernatant was purified using 1 ml Ni-NTA columns (Macherey-Nagel). His-tagged UL12 was eluted using lysis buffer with increasing concentration of Imidazole (100 mM ...
-
bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
-
bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from tissues including apical notches in 4-day-old gemmalings using NucleoSpin RNA Plant (Macherey-Nagel, Duren, Germany) according to manufacturer’s instruction ...
-
bioRxiv - Cancer Biology 2023Quote: ... or 1.2 l LB-medium and incubated for 4 h at 37 °C before extracting plasmid DNA using Nucleobond Xtra Midi kit (Macherey-Nagel, C640003) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2023Quote: ... The samples (40 μl) were injected onto a MN Nucleosil C18 analytical column (250 mm x 4 mm i.d., 100-5) (Macherey-Nagel, Duren, Germany), using a flow rate of 0.8 mL.min-1 at 27 °C ...