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Citations for Macherey-Nagel GmbH :
101 - 149 of 149 citations for Dengue Virus Serotype 4 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
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bioRxiv - Cell Biology 2020Quote: ... All plasmids were purified from 4 ml bacterial culture using either NucleoSpin® Plasmid (REF 740588.50, Macherey-Nagel®) or PureLink™ HiPure Plasmid Miniprep Kit (Art ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and 100 µL of the supernatant was collected in gaschromatography (GC) glass vials (Macherey-Nagel, Düren, Germany); both were stored at -80°C ...
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bioRxiv - Bioengineering 2022Quote: ... Germany) with a guard column (EC 4/3 NUCLEODUR C18 Gravity, 3 μm, Macherey-Nagel GmbH & Co. KG Germany), while the column temperature was kept at 40 °C in a thermostatic column compartment (Thermo Fisher UltiMate 3000) ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then purified from bacterial extracts by affinity chromatography using glutathione-conjugated agarose (Macherey-Nagel), followed by cleavage of the GST-tag with PreScission protease ...
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bioRxiv - Systems Biology 2022Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Immunology 2022Quote: Total RNA from cultured cells was extracted using the Nucleospin RNA/Protein kit (Macherey-Nagel, Düren, Germany). RNA was retrotranscribed using SuperScript III reverse transcriptase (Invitrogen ...
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bioRxiv - Systems Biology 2022Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Biochemistry 2023Quote: ... SUV420H1 proteins were isolated by incubating the soluble fraction for 2 hrs with GST beads (Macherey-Nagel). The beads were washed with two column volumes of wash buffer (20 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The protein samples resolved in agarose gel were vacuum-transferred onto the nitrocellulose membrain (Porablot, MACHEREY-NAGEL), and subjected to a standard Western-Blotting procedure with either anti-Sup35NM ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Biochemistry 2024Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Immunology 2021Quote: ... Virus was purified by centrifugation at 12,000 xg for 10 mins at 4 °C and genomic DNA extracted (NucleoSpin Tissue kit, Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Biophysics 2019Quote: ... centrifuged (60,000xg, 45min at 4°C) and the supernatant was incubated with Nickel agarose beads (Protino Ni-TED, Macherey-Nagel) for 1h at 4°C.
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected onto an RP18 column (4 × 50 mm, 2.7 μm particle size, Nucleoshell, Macherey-Nagel) with a flow rate of 0.25 mL min−1 ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from the spleen of 4-6 weeks-old asymptomatic mice using nucleospin RNA II (Macherey-Nagel). The quality of RNA was checked with Bioanalyzer Agilent 2100 and RNAs with a RIN (RNA integrity number ...
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bioRxiv - Plant Biology 2024Quote: ... Samples (80 µL) were separated on a NUCLEOSIL® C18 column (EC 250/4, 300-5, MACHEREY-NAGEL GmbH, Germany) in a LaChrome Elite HPLC equipped with an L-2130 pump module ...
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bioRxiv - Biochemistry 2020Quote: ... After proteolytic cleavage the protein was incubated with 1.5 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) for 25 min at 4°C to remove His6-SUMO (for purification of SUMO-Hsc70 overnight digestion with Ulp1 and second incubation with Protino Ni-IDA was omitted) ...
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bioRxiv - Molecular Biology 2021Quote: Complementary DNAs were obtained by reverse transcription of total RNA using the NucleoSpin RNA/Protein kit (Macherey-Nagel) and used as templates for PCR amplification using Brilliant III Ultra-Fast QPCR Master Mix (Agilent ...
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bioRxiv - Genetics 2020Quote: ... RNA and protein were simultaneously extracted from pellets using the NucleoSpin TriPrep Kit (Macherey-Nagel, supplied by ThermoFisher). Deletion of the target region was confirmed by end-point of DNA using primers flanking the guides (primers 5’-AGGGGTTGAGCTGGATTCC-3’ and 5’-CCAGCACCCACTCTGTAGAT-3’ ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total mRNA was extracted from leaf tissue with a NucleoSpin RNA/Protein kit (REF740933, Macherey-Nagel GmbH & Co.), then treated with DNase before transcription into cDNA using a Superscript III First-Strand Synthesis System (18080-051 ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were run on bis-acrylamide gels and then transferred to PVDF membranes (Macherey-Nagel, Düren, Germany) using a Trans-blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Systems Biology 2021Quote: ... the amplified plasmid library was extracted from bacterial cells using 4 reactions of Midiprep kit (Macherey-Nagel NucleoBond Xtra Midi Plus).
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was separated from the debris by centrifugation at 4000g for 40 minutes at 4°C and the supernatant was purified using 1 ml Ni-NTA columns (Macherey-Nagel). His-tagged UL12 was eluted using lysis buffer with increasing concentration of Imidazole (100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... After cell lysis using a French press the cell debris was removed by centrifugation at 17000 g for 1 h at 4 °C and the cleared lysate was incubated with Protino IDA resin (Macherey-Nagel) for 20 min at 4° C ...
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were supplemented with 20 mM imidazole and incubated with Protino Ni-NTA agarose beads (Macherey-Nagel) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from cell-free lysate by metal ion affinity chromatography with Protino Ni-NTA Agarose (Macherey-Nagel) as matrix followed by ultrafiltration (Amicon Ultra-15 centrifugal filter units ...
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bioRxiv - Biochemistry 2021Quote: ... and the GST fusion proteins were purified using Protino gluthathione agarose 4B according to the manufacturer’s instruction (Macherey-Nagel). SPR interaction studies were performed according to previous studies46,85 ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of plants 5 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins from the soluble fraction were purified by affinity chromatography with Protino Ni-TED PackedColumns2000 (Macherey-Nagel, Düren, Germany) based on the His-tag tail ...
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bioRxiv - Molecular Biology 2024Quote: ... Freezing and RNA isolation was done following the instructions of the NucleoSpin RNA/Protein Kit (Macherey-Nagel; Düren, Germany). 1 µg of the extracted total RNA per sample was reverse transcribed using oligo(dt)18 primers (First Strand cDNA Synthesis Kit ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from tissues including apical notches in 4-day-old gemmalings using NucleoSpin RNA Plant (Macherey-Nagel, Duren, Germany) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... or 1.2 l LB-medium and incubated for 4 h at 37 °C before extracting plasmid DNA using Nucleobond Xtra Midi kit (Macherey-Nagel, C640003) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... The samples (40 μl) were injected onto a MN Nucleosil C18 analytical column (250 mm x 4 mm i.d., 100-5) (Macherey-Nagel, Duren, Germany), using a flow rate of 0.8 mL.min-1 at 27 °C ...
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bioRxiv - Neuroscience 2019Quote: ... RNA samples were obtained from the cell pellet using the RNA-protein extraction kit as specified by manufacturer (Macherey-Nagel). Library construction and RNA sequencing were performed by the Duke-NUS Genome Biology Facility ...
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bioRxiv - Biochemistry 2023Quote: Pull-down experiments using GST (Glutathion-S-Transferase)-fused proteins were performed using glutathione agarose beads (Macherey-Nagel, Düren, Germany). The beads were incubated with the GST-fused proteins for 1 h ...
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bioRxiv - Biochemistry 2024Quote: ... The CBP-His6-ZZ-Upf1-HD proteins were first purified on a Protino® Ni-NTA agarose resin (Macherey-Nagel). After successive washing steps using buffers C200 first ...
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bioRxiv - Biochemistry 2020Quote: ... 4°C for 45 min) and the supernatant was incubated for 25 min at 4°C with 2 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
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bioRxiv - Molecular Biology 2019Quote: ... Twenty microliters have been collected for protein analysis and RNA was extracted from remaining supernatant with the Nucleospin RNA extraction II Kit (Macherey-Nagel).
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bioRxiv - Neuroscience 2022Quote: ... and 1:8 of His-SUMO-CREB and MBP-Jacob-45-228) were incubated with GST-LMO4 and GST (control protein) immobilized on Protino Glutathione Agarose 4B beads (Macherey-Nagel). Probes were eluted with 25µl of 2x SDS sample buffer after incubation and washing steps (washing buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of respective proteins from bacterial extracts was performed by affinity chromatography using Protino glutathione-conjugated agarose 4B (Macherey-Nagel, Düren, Germany). Except for GST-WAVE2-WCA ...
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bioRxiv - Neuroscience 2020Quote: ... The total proteins were precipitated and prepared for Polyacrylamide Gel Electrophoresis using protein precipitator and resuspension buffer PSB/TCEP (Protein solving buffer and (tris(2-carboxyethyl)phosphine) TCEP reducing agent) from the NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France). The elution fraction containing RNA was further used for PCR analysis (see below) ...
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bioRxiv - Plant Biology 2021Quote: ... in pyridine followed by a 30 min treatment at 37 °C with 100 μI of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). For each sample ...
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bioRxiv - Plant Biology 2022Quote: ... in pyridine followed by a 30 min treatment at 37 °C with 70 μl of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). An autosampler Gerstel Multi-Purpose system (Gerstel GmbH & Co.KG ...
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bioRxiv - Plant Biology 2023Quote: ... in pyridine followed by a 30 min treatment at 37°C with 70 μl of N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). An autosampler Gerstel Multi-Purpose system (Gerstel GmbH & Co ...