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Citations for Macherey-Nagel GmbH :
501 - 550 of 713 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were purified with a NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Restriction enzymes and T4 DNA ligase were obtained from Thermo Fisher Scientific and were used according to the supplier’s specifications ...
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bioRxiv - Genetics 2021Quote: ... The PCR amplicon was gel-purified by using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified from agarose gels using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). EIF2AK2 and EIF2S1 sequences were inserted into pET-His 1a vector using the Gibson Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from 2.5×105 HEK293T cells was purified using a Nucleo Spin Tissue XS kit (MACHEREY-NAGEL). Using 25 mg of human postmortem tissue ...
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bioRxiv - Bioengineering 2022Quote: ... DNA was purified from PCR mixtures or agarose gels using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). The purity and concentration of DNA were checked by NanoDrop 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... The products were inspected on agarose gel and purified using Nucleofast PCR purification plates (Macherey-Nagel, Dueren, Germa) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from the cells with a PGTX solution42 and purified using NucleoSpin RNA (MACHEREY-NAGEL). cDNA was synthesized from 1 μg of total RNA using ReverTra Ace qPCR RT Master Mix (TOYOBO) ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used in cloning reactions were purified using the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the DNA of HEK293 cells was extracted and purified following the NucleoSpin Tissue protocol (Macherey-Nagel, Düren, Germany). The genotype at rs1047626 was verified to be heterozygote (AG ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM PMSF and purified by immobilized metal affinity chromatography using a 5 ml nickel column (Macherey-Nagel). The protein was eluted from the nickel column with solubilization buffer supplemented with 500 mM imidazole ...
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bioRxiv - Genomics 2023Quote: ... The DNA donors were then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and stored at -20°C or directly used for electroporation ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified by a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel., Düren, Germany). COI gene fragments were amplified from some samples according to the USDA recommended protocol (https://www.fsis.usda.gov/sites/default/files/media_file/2020-11/CLG-FPCR.pdf ...
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bioRxiv - Molecular Biology 2023Quote: ... and the reverse transcription products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). The reverse transcription products were eluted with 30 μL of HiDi formamide (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from tissues or purified cells using the the NucleoSpin® RNA Plus Kit (Macherey-Nagel). Complementary DNAs were synthesized using the SuperScript™ III First-Strand Synthesis System Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were subsequently purified using the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting amplicons were pooled and gel purified using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Samples were sequenced on a MiSeq platform with ∼10 million reads per sample (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were purified via gel extraction using a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). Illumina sequencing adapters were added with Phusion High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Immunology 2023Quote: ... purified again via agarose gel separation and using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany), before being pooled and submitted to GATC Biotech (Germany) ...
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bioRxiv - Bioengineering 2023Quote: ... PCR samples were then purified using following the manufacturer’s instructions (NucleoSpin® PCR and gel kit, Macherey-Nagel). Purified amplicons were verified by electrophoresis on a 1% agarose gel ...
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bioRxiv - Genetics 2023Quote: ... PCR reactions were purified using a PCR/Gel purification kit (NucleoSpin Gel and PCR Clean□up, Macherey-Nagel).
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bioRxiv - Genetics 2023Quote: ... All probes were gel purified using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel, cat# 740609). The pixel intensities of the bands corresponding to linear ...
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bioRxiv - Genetics 2023Quote: ... the fragments were purified using a PCR/Gel purification kit (NucleoSpin Gel and PCR Clean□up, Macherey-Nagel) and sequenced using the smt0-seq-Rv oligonucleotide ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were purified with the rDNase set and the RNA Clean-up kit (Macherey-Nagel, Düren, Germany). RNA quality was checked with the Prokaryote Total RNA Nano Series II chip using the Agilent Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments and PCR products were purified using the Nucleospin Gel and PCR clean up kit (Macherey-Nagel).
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bioRxiv - Developmental Biology 2024Quote: ... RNA was harvested at day 14 of the protocols and purified using the Nucleo Spin RNA (Macherey-Nagel) kit according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... RNA was column purified using NTC buffer and the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions.
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... were grown overnight at 37 °C and plasmid was subsequently purified using the NucleoBond Xtra Midi kit (Macherey-Nagel). Plasmid DNA was solubilized in 150 µl of TE buffer and quantified using NanoDrop ND-1000 spectrophotometer (Celbio) ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplicons were purified using the NucleoSpin® Gel and PCR clean up kits from Macherey-Nagel (Bethlehem, PA), after which quantification and qualification of purified band(s ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... All cRNA samples were purified with the NucleoSpin RNA Clean-up system (Macherey-Nagel, GmbH & Co. KG, Düren, Germany) and quantified on a Nano-Drop spectrophotometer ND-2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Bacteria were collected and the plasmid DNA was purified using NucleoBond® Xtra Midi kit (MACHEREY-NAGEL, cat. 740410.50). The transformation efficiency was estimated by plating 1/10000 of the transformation reaction onto 15 cm LB-agar plate with 100 µg/ml ampicillin and counting number of clones after incubation overnight at 37°C.
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA were purified after 72 hours incubation with a Nucloespin RNA purification kit (Macherey-Nagel cat. no. 740955) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... purified according to the instructions of the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel GmbH, Düren, Germany) and cloned into the pET22b(+ ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the PCR products were purified using a NucleoSpin Gel and a PCR Clean-up Kit (Macherey-Nagel, Duren, Germany) and then sequenced directly by Sanger sequencing using the same PCR primers ...
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bioRxiv - Biochemistry 2020Quote: ... Immunoprecipitated DNA was purified with NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The vRNA in the cell culture supernatant was purified using a NucleoSpin® RNA virus kit (Macherey-Nagel, 740956) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... ChIP-DNA and input DNA were purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel). The DNA concentration of the input DNA was determined using the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The amplified rDNA gene fragments were purified using nucleobond AX 100 columns for Miniprep System (Macherey-Nagel, ref 740521.100). The rDNA sequences were then labeled with Digoxigenin-11-dUTP by nick translation according to the manufacturer’s protocol (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... All plasmids were purified from 4 ml bacterial culture using either NucleoSpin® Plasmid (REF 740588.50, Macherey-Nagel®) or PureLink™ HiPure Plasmid Miniprep Kit (Art ...
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bioRxiv - Developmental Biology 2022Quote: ... In vitro transcribed mRNAs were purified using Macherey-Nagel Nucleospin® RNA Clean-up kit (Macherey-Nagel, Bethlehem, PA) prior to injections.
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bioRxiv - Microbiology 2022Quote: ... A total of 400 µl of this PCR was run and purified using column-based clean-up (Macherey-Nagel).
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bioRxiv - Immunology 2021Quote: RNA of polyclonally activated CD8+ T cells from C57BL6/N mice was purified using NucleoSpin RNA kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from cell-free lysate by metal ion affinity chromatography with Protino Ni-NTA Agarose (Macherey-Nagel) as matrix followed by ultrafiltration (Amicon Ultra-15 centrifugal filter units ...
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bioRxiv - Biochemistry 2021Quote: ... and the GST fusion proteins were purified using Protino gluthathione agarose 4B according to the manufacturer’s instruction (Macherey-Nagel). SPR interaction studies were performed according to previous studies46,85 ...
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bioRxiv - Genomics 2021Quote: ... PCR was performed and the products were purified with a NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL).
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bioRxiv - Molecular Biology 2021Quote: ... DNA fragments were purified from agarose gels using the NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel. Genomic DNA extractions from Amycolatopsis and E ...
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bioRxiv - Immunology 2022Quote: ... RNA from dry pellets of Vγ9Vδ2 T lymphocytes was extracted and purified using the NucleoSpin RNA plus isolation kit from Macherey-Nagel. DGE-sequencing was performed by the BIRD core facility and data were analyzed using R programming languages and tools.
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the adeD PCR product was additionally gel-purified (NucleoSpin Gel and PCR Clean-Up Kit, Macherey-Nagel, Düren, DE), and 100 ng of purified DNA was subsequently incubated with 0.2 M dATP and 5 U DreamTaq Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Resulting PCR products were purified from 1% agarose gels with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel) to prevent the production of side products during in vitro transcription ...