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Citations for Macherey-Nagel GmbH :
51 - 100 of 433 citations for Cell Division Cycle 2 Like Protein Kinase 6 CDC2L6 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
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bioRxiv - Genetics 2019Quote: ... Cells were then treated with JQ1 at the effective IC50 value for 6 hours and RNA was harvested using NucleoSpin RNA Plus columns (Macherey-Nagel). RNA expression levels were quantified after reverse transcription with MMLV H-(Promega ...
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bioRxiv - Plant Biology 2023Quote: PCR products were pooled by 6 to 12 according to the linkers and cleaned using the Nucleospin Gel and PCR clean up (Macherey-Nagel). Samples containing asymptomatic plants were pooled separately from those containing symptomatic leaves to limit potential cross-contaminations of highly concentrated viruses in symptomatic pools ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified using Protino NTA columns (Macherey-Nagel, Freemansburg, PA, USA) using linear imidazole gradients (30-250 mM) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified at 4 °C on Ni-NTA-Sepharose (Macherey-Nagel) packed in a gravity flow column ...
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bioRxiv - Immunology 2022Quote: ... The protein was purified on a Ni-NTA resin column (Macherey-Nagel) and the eluted fractions were concentrated and dialyzed against PBS using the same Amicon system as described above ...
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bioRxiv - Cell Biology 2019Quote: mRNA was isolated using the NucleoSpin® RNA/Protein kit (Macherey-Nagel). Purified mRNA was transcribed into cDNA with the QuantiTect®Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were then transferred onto Porablot NCP nitrocellulose membrane (Macherey-Nagel) and blocked for 1 hour at RT in 3% BSA (Applichem ...
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bioRxiv - Genetics 2019Quote: ... 8 pairs of ovaries were dissected from 6 day-old virgin females and total RNA were extracted using the NucleoSpin® RNA isolation kit (Macherey-Nagel), following the instructions of the manufacturer ...
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bioRxiv - Developmental Biology 2022Quote: gDNA from 6 male and 5 female pupae (sexed by eye) was extracted using the NucleoSpin Tissue kit (Macherey-Nagel, Düren, Germany), pooled by sex and used as template for subsequent PCRs ...
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bioRxiv - Cancer Biology 2019Quote: Cells pellet (1.5*10^6) were collected at different time points after LPS stimulation and DNA was extracted with the Nucleospin tissue kit (Macherey-Nagel, 740952). The genomic DNA was eluted in 50 μl of BE buffer (5 mM Tris/HCl ...
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bioRxiv - Systems Biology 2023Quote: ... equipped with a 30-m OPTIMA® 1301-MS column (6% cyanopropylphenyl, 94% dimethylpolysiloxane, 0.25 mm internal diameter, 0.25 µm film thickness; Macherey-Nagel, Düren, Germany). Injections of 1 µL aliquots of sample solution were performed in splitless or split mode depending on the peak intensities (n = 2 ...
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bioRxiv - Biochemistry 2023Quote: ... The GST fusion proteins were purified with Protino Glutathione Agarose 4B (Macherey-Nagel) and GST was cleaved by human thrombin (Biopharm laboratories ...
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bioRxiv - Plant Biology 2024Quote: ... The protein was purified using Protino Ni-NTA agarose (Macherey-Nagel, #7450400-500).
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was isolated from peripheral blood and tail tissues of each chimeric mouse 6 weeks after BM transplantation with NucleoSpin® Tissue Kit (MACHEREY-NAGEL, Duren, Germany). Then ...
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bioRxiv - Microbiology 2023Quote: ... inoculated seeds and seedlings of the SynCom conditions (control, 6-strain, 8-strain, 12-strain SynComs) with the NucleoSpin® 96 Food kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2019Quote: ... Proteins were captured using glutathione-agarose column (Protino glutathione-agarose 4B, Macherey-Nagel Gmbh) equilibrated with 20 mM Tris HCl ...
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bioRxiv - Neuroscience 2020Quote: ... Protein and RNA extractions were performed with NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The Psyn ThyX recombinant protein was purified on Protino Ni-TED column (Macherey-Nagel) as previously described49 ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted using the total RNA and protein isolation kit (Macherey-Nagel). RNA samples were retrotranscribed (High-Capacity cDNA Reverse Transcription kit ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NuleoSpin® RNA/Protein extraction kit (740933.50) was purchased from Macherey-Nagel (Hoerdt, France). Sequencing grade modified trypsin (V5111) ...
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bioRxiv - Microbiology 2020Quote: ... Protein was purified by SPE with a Chromabond SPE-C4 cartridge (Macherey-Nagel, Germany). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted with the Nucleospin RNA/Protein kit (Macherey-Nagel, Düren, DE) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleosil 100-5 N(CH3)2 column (Macherey-Nagel GmbH & Co, Düren, Germany) and eluted isocratically with 100% methanol containing 0.01% acetic acid at a flow rate of 1 ml min-1 ...
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bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
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bioRxiv - Biophysics 2022Quote: ... and the protein was affinity- purified from lysate using Ni-NTA affinity resin (Macherey-Nagel) and elution with imidazole ...
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bioRxiv - Microbiology 2023Quote: ... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were purified from the cleared lysate with Ni-NTA agarose (Macherey-Nagel, Düren, Germany) following concentration and dialysis against 0.1 M potassium phosphate buffer pH 7.
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bioRxiv - Neuroscience 2022Quote: RNA used for sequencing was extracted from tissue using the NucleoSpin RNA/Protein Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-tagged proteins in soluble extracts were purified by GSH-agarose beads (Macherey-Nagel, Düren, Germany) from which were eluted by the addition of 50 mM reduced L-glutathione (dissolved in 25 mM Tris-HCl pH 8).
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bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then purified from bacterial extracts by affinity chromatography using glutathione-conjugated agarose (Macherey-Nagel), followed by cleavage of the GST-tag with PreScission protease ...
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bioRxiv - Systems Biology 2022Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Systems Biology 2022Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Biochemistry 2023Quote: ... The protein samples resolved in agarose gel were vacuum-transferred onto the nitrocellulose membrain (Porablot, MACHEREY-NAGEL), and subjected to a standard Western-Blotting procedure with either anti-Sup35NM ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Biochemistry 2024Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Biochemistry 2020Quote: ... After proteolytic cleavage the protein was incubated with 1.5 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) for 25 min at 4°C to remove His6-SUMO (for purification of SUMO-Hsc70 overnight digestion with Ulp1 and second incubation with Protino Ni-IDA was omitted) ...
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bioRxiv - Molecular Biology 2021Quote: Complementary DNAs were obtained by reverse transcription of total RNA using the NucleoSpin RNA/Protein kit (Macherey-Nagel) and used as templates for PCR amplification using Brilliant III Ultra-Fast QPCR Master Mix (Agilent ...
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bioRxiv - Genetics 2020Quote: ... RNA and protein were simultaneously extracted from pellets using the NucleoSpin TriPrep Kit (Macherey-Nagel, supplied by ThermoFisher). Deletion of the target region was confirmed by end-point of DNA using primers flanking the guides (primers 5’-AGGGGTTGAGCTGGATTCC-3’ and 5’-CCAGCACCCACTCTGTAGAT-3’ ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were run on bis-acrylamide gels and then transferred to PVDF membranes (Macherey-Nagel, Düren, Germany) using a Trans-blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total mRNA was extracted from leaf tissue with a NucleoSpin RNA/Protein kit (REF740933, Macherey-Nagel GmbH & Co.), then treated with DNase before transcription into cDNA using a Superscript III First-Strand Synthesis System (18080-051 ...
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bioRxiv - Plant Biology 2021Quote: ... a Nucleoshell RP 18 plus reversed-phase column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Developmental Biology 2022Quote: ... The supernatant was collected and loaded onto 2 mL pre-equilibrated Ni-NTA agarose (Macherey-Nagel). The mixture was rotated at 4 □ for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... elegans animals were transferred to 2 ml MN Bead Tubes Type A (Macherey-Nagel, Düren, Germany) and lysed using a Precellys Bead Beating system with an additional Cryolys cooling module (Bertin Instruments ...
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bioRxiv - Cancer Biology 2023Quote: ... BM DOHH2 were resuspended in RA1 buffer and tris(2-carboxyethyl)phosphine (TCEP) from Macherey-Nagel and stored at -80°C for less than 3 months ...
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were supplemented with 20 mM imidazole and incubated with Protino Ni-NTA agarose beads (Macherey-Nagel) for 1 hour at 4°C ...