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Citations for Macherey-Nagel GmbH :
1 - 50 of 2292 citations for Bicinchoninic Acid BCA Protein Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration was quantified using the Protein Quantification Assay kit (Macherey-Nagel). The protein samples were resolved using 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gels (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... The protein concentration was determined by Protein Quantification Assay (Cat.# 740967,Macherey-Nagel). 10 µg of the protein was separated by SDS-PAGE electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... All protein concentrations were determined with the MN Protein Quantification Assay (Macherey-Nagel). Western blotting was performed utilizing AnykD Mini-PROTEAN TGX Gels (Biorad ...
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bioRxiv - Genomics 2024Quote: ... RNA & Protein Purification kit (Macherey-Nagel). Clones were screened by XhoI digestion and Sanger sequencing.
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bioRxiv - Cell Biology 2023Quote: ... and protein isolation kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and protein extraction kit (Macherey-Nagel). RNA was further purified by using RNA Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Plant Biology 2024Quote: ... Protein quantification was performed using the protein quantification Kit from Macherey-Nagel. Samples were loaded on an equal protein basis (5-10 μg per well depending on the experiment) ...
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bioRxiv - Cancer Biology 2024Quote: The Nucleospin RNA/Protein Kit (Macherey-Nagel) was used to extract RNA and also proteins from siRNA validation experiments (Fig ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Developmental Biology 2023Quote: ... and protein extraction kit (Macherey-Nagel, Allentown, PA) (gene expression studies ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein samples were extracted from the organs with the NucleoSpin RNA/Protein kit (Macherey-Nagel). Macherey-Nagel Bead Tubes Type F was used in tissue homogenization ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted using the pathogen Nucleic Acid Extraction kit (Macherey-Nagel, Düren, Germany) and eluted in 50 μL elution buffer ...
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bioRxiv - Immunology 2021Quote: ... total protein was isolated by NucleoSpin TriPrep kit (Macherey-Nagel) from 5×106 PMNs ...
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bioRxiv - Immunology 2024Quote: ... and RNA was isolated using a Mini Kit for RNA and protein purification (NucleoSpin RNA/Protein, Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: Total protein was extracted from differentiated neurons at DIV 35 with the NucleoSpin RNA/Protein Kit (Macherey-Nagel 740933) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA and proteins (used for the proteomic study) were extracted using the NucleoSpin® RNA/Protein extraction kit (Macherey-Nagel). RNA quantification was performed using a Nanodrop® spectrophotometer (ND-1000) ...
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bioRxiv - Cancer Biology 2024Quote: After total RNA extraction using the Nucleospin RNA/Protein Kit (Macherey-Nagel) and quantification using NanoDrop™ 2000 (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was extracted (DNA, RNA and protein purification Kit, #74098 Macherey-Nagel) and then converted to cDNA (High Capacity cDNA Reverse Transcription ...
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bioRxiv - Genetics 2021Quote: ... Nucleic acid extraction was performed with the NucleoSpin DNA Insect Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Protein and mRNA were extracted from the same leaf sample (NucleoSpin RNA/Protein kit, REF740933, Macherey-Nagel GmbH & Co., Düren, Germany).
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bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from ethylenediaminetetraacetic acid (EDTA)-anticoagulated blood using NucleoSpin Blood Quick Pure kit (Macherey-Nagel, Duren, Germany) and was stored in the gene bank run by Azabu University (Kanagawa ...
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bioRxiv - Plant Biology 2024Quote: ... (2017) and using the nucleospin RNA/Protein kit (Macherey-Nagel, http://www.mn-net.com/). Protein quantification was performed using the protein quantification Kit from Macherey-Nagel ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Neuroscience 2020Quote: ... Protein and RNA extractions were performed with NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NuleoSpin® RNA/Protein extraction kit (740933.50) was purchased from Macherey-Nagel (Hoerdt, France). Sequencing grade modified trypsin (V5111) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted with the Nucleospin RNA/Protein kit (Macherey-Nagel, Düren, DE) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was isolated utilizing an RNA and protein purification kit (Macherey-Nagel, Düren, Germany) as per the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: RNAs were extracted and purified from samples using NucleoSpin RNA/Protein kit (Macherey-Nagel). Reverse transcription was performed with PrimeScript RT reagent kit containing gDNA Eraser (Takara Bio Europe ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted using the total RNA and protein isolation kit (Macherey-Nagel). RNA samples were reverse-transcribed with High-Capacity cDNA Reverse Transcription reagents kit (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: Ribonucleic acid (RNA) samples were isolated from seeds and leaves using the NucleoSpin® RNA Plant kit (Macherey-Nagel, Düren, Germany) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were used for functional assays or RNA isolation by NucleoSpin RNA isolation kit (Macherey-Nagel, Germany). In total ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted using Nucleospin RNA/protein kit following the manufacturer protocol (Macherey-Nagel, Germany). Reverse transcriptase reaction was performed using 500–1000ng of RNA ...
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bioRxiv - Neuroscience 2022Quote: RNA used for sequencing was extracted from tissue using the NucleoSpin RNA/Protein Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Total RNA from cultured cells was extracted using the Nucleospin RNA/Protein kit (Macherey-Nagel, Düren, Germany). RNA was retrotranscribed using SuperScript III reverse transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: Complementary DNAs were obtained by reverse transcription of total RNA using the NucleoSpin RNA/Protein kit (Macherey-Nagel) and used as templates for PCR amplification using Brilliant III Ultra-Fast QPCR Master Mix (Agilent ...
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bioRxiv - Genetics 2020Quote: ... RNA and protein were simultaneously extracted from pellets using the NucleoSpin TriPrep Kit (Macherey-Nagel, supplied by ThermoFisher). Deletion of the target region was confirmed by end-point of DNA using primers flanking the guides (primers 5’-AGGGGTTGAGCTGGATTCC-3’ and 5’-CCAGCACCCACTCTGTAGAT-3’ ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total mRNA was extracted from leaf tissue with a NucleoSpin RNA/Protein kit (REF740933, Macherey-Nagel GmbH & Co.), then treated with DNase before transcription into cDNA using a Superscript III First-Strand Synthesis System (18080-051 ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of plants 5 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Molecular Biology 2024Quote: ... Freezing and RNA isolation was done following the instructions of the NucleoSpin RNA/Protein Kit (Macherey-Nagel; Düren, Germany). 1 µg of the extracted total RNA per sample was reverse transcribed using oligo(dt)18 primers (First Strand cDNA Synthesis Kit ...
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bioRxiv - Plant Biology 2024Quote: Leaf discs of the relevant transgenic and azygous lines were ground in dry ice and RNA extractions were performed following the nucleospin RNA/Protein kit (Macherey-Nagel). RNAseq was performed by Novogene ...
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bioRxiv - Neuroscience 2024Quote: Snap frozen ipsi- and contralateral brain hemispheres were homogenized using ceramic beads (PeriCellyse system, Bertin Technology) and RNA and protein simultaneously purified with the Nucleospin® TriPrep kit (Macherey-Nagel) according to the manufactureŕs instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The total proteins were precipitated and prepared for Polyacrylamide Gel Electrophoresis using protein precipitator and resuspension buffer PSB/TCEP (Protein solving buffer and (tris(2-carboxyethyl)phosphine) TCEP reducing agent) from the NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France). The elution fraction containing RNA was further used for PCR analysis (see below) ...
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bioRxiv - Biochemistry 2024Quote: All GST-fusion proteins were purified with soluble protein fractions bound to Protino Glutathione Agarose resin 4B (GSH resin, Macherey-Nagel). For Isothermal Titration Calorimetry (ITC) ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved protein was passed through Ni-TED (Macherey-Nagel) to remove uncleaved protein ...
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bioRxiv - Biochemistry 2024Quote: All proteins were purified using Ni-IDA (Macherey-Nagel) following the instructions provided by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... the soluble fraction was directly loaded onto a 1-ml nickel column (Protino Ni-nitrotriacetic acid [NTA]; Macherey-Nagel). The column was washed with Wash Buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The sample was then acidified using trifluoroacetic acid and peptides were purified using Chromabond C18 Microspin columns (Macherey-Nagel).
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bioRxiv - Microbiology 2023Quote: The nucleic acids were extracted from the FFPE tissue sections using the Nucleospin total RNA FFPE XS (Macherey-Nagel). RNA sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Synthetic Biology 2022Quote: ... SLS was precipitated with trifluoroacetic acid (TFA, 1.5% final concentration) and peptides were purified using Chromabond C18 microspin columns (Macherey-Nagel). Acidified peptides were loaded on spin columns equilibrated with 400 μL acetonitrile and then 400 μL 0.15% TFA ...