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Citations for Macherey-Nagel GmbH :
1 - 50 of 152 citations for Aminomethylphosphonic Acid 13C 99%;15N 98%;Methylene D2 98% 100 Ug Ml H2O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from only the 13C-acetate grown cultures that yielded enough DNA at 94 days of incubation using the NucleoSpin Microbial DNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the soluble fraction was directly loaded onto a 1-ml nickel column (Protino Ni-nitrotriacetic acid [NTA]; Macherey-Nagel). The column was washed with Wash Buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2024Quote: ... The fixed cells were washed twice with PBS and stained with 20 µg/mL propidium iodide (PI) containing 100 mg/mL RNAse A (740505, MACHEREY-NAGEL) for 15 min at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... The pellet was washed once with sterile H2O and then each library was purified over two columns of the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany). A total of 17 Mio were harvested ...
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bioRxiv - Microbiology 2021Quote: ... The supercoiled pUC19 (pUC19sc) was extracted from 100 ml overnight cultures using the NucleoBond Xtra Midi kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... coli strain in presence of 100 μg/ml ampicillin and prepared by Midi DNA-prep kit (Macherey-Nagel, 740410) for the transient transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA in the supernatant was precipitated with isopropanol and suspended in 250 μL of STE (10 mM Tris-HCl [pH 8.0], 1 mM EDTA [pH 8.0], 100 mM NaCl containing 80 μg/mL RNaseA [Macherey-Nagel]). RNA was digested by incubation for 1 hr at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: 100 µL pyridine and 100 µL MSTFA (Macherey-Nagel) were added to dried yeast extracts and incubated for 2 h at 70 °C.
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was eluted from HisPur Cobalt Resin by incubating samples in a final volume of 100 µl of PBS with 0.4 mg/ml of Proteinase K (Macherey-Nagel, #740506) and incubated for 2 h at 65°C with shaking at 800 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel). The selection markers for fungal transformation were cloned into digested plasmids by using the Rapid DNA Ligation Kit (Thermo) ...
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bioRxiv - Molecular Biology 2023Quote: ... positive colonies were cultured in 100 ml of 2YT medium and prepped using a midi-scale commercial kit (Macherey-Nagel 740410100).
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bioRxiv - Plant Biology 2021Quote: ... an EC 100/2 Nucleoshell Phenyl-Hexyl column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted using the pathogen Nucleic Acid Extraction kit (Macherey-Nagel, Düren, Germany) and eluted in 50 μL elution buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Spheroids were transferred into PCR-clean 1.5 mL microtubes with 100 µL of lysis buffer (LB1 from Macherey-Nagel or 700 µL of Buffer RLT (79216, Qiagen). Lysates were snap-frozen and stored at −80 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... following which it was applied onto a pre-equilibrated (EQ buffer containing 0.15% Triton X-100) Nucleobond AX-100 column (Macherey-Nagel). The column-bound RNA was washed twice with wash buffer WB (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:100 RNase A (MACHEREY-NAGEL, 740505) was added in each sample for 15 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Genetics 2021Quote: ... Nucleic acid extraction was performed with the NucleoSpin DNA Insect Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from ethylenediaminetetraacetic acid (EDTA)-anticoagulated blood using NucleoSpin Blood Quick Pure kit (Macherey-Nagel, Duren, Germany) and was stored in the gene bank run by Azabu University (Kanagawa ...
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bioRxiv - Microbiology 2023Quote: The nucleic acids were extracted from the FFPE tissue sections using the Nucleospin total RNA FFPE XS (Macherey-Nagel). RNA sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... The sample was then acidified using trifluoroacetic acid and peptides were purified using Chromabond C18 Microspin columns (Macherey-Nagel).
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bioRxiv - Synthetic Biology 2022Quote: ... SLS was precipitated with trifluoroacetic acid (TFA, 1.5% final concentration) and peptides were purified using Chromabond C18 microspin columns (Macherey-Nagel). Acidified peptides were loaded on spin columns equilibrated with 400 μL acetonitrile and then 400 μL 0.15% TFA ...
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bioRxiv - Plant Biology 2021Quote: Ribonucleic acid (RNA) samples were isolated from seeds and leaves using the NucleoSpin® RNA Plant kit (Macherey-Nagel, Düren, Germany) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting bottom organic phase was dried and on-line derivatized to fatty acid methyl ester by a chloroform/methanol (1:2)-TMSH solution (Macherey-Nagel) and analyzed by gas chromatography-mass spectrometry (Agilent 5977A-7890B) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1mg/mL proteinase K (Macherey-Nagel) and then with 0.1 mg/mL RNaseA (Qiagen ...
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bioRxiv - Plant Biology 2023Quote: ... then added 1.2 µL of RNase (MN, Macherey-Nagel, Ref. 740505, 1200 U/mL, 10 mg/mL), and transferred the DNA to a 96-well KingFisher Flex plate (ThermoFischer Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were resuspended in PBS with 20 µg/ml PI and 0.2 mg/mL RNAse A (Macherey-Nagel) and incubated at RT for 30 min before analysis on a FACSCalibur (Beckton Dickinson) ...
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bioRxiv - Biochemistry 2021Quote: ... with an EC 250/4 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel) with a general gradient of 10% to 90% ACN in H2O ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleosil 100-5 N(CH3)2 column (Macherey-Nagel GmbH & Co, Düren, Germany) and eluted isocratically with 100% methanol containing 0.01% acetic acid at a flow rate of 1 ml min-1 ...
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bioRxiv - Microbiology 2019Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Macherey-Nagel #740579) and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... placed in 100 μl of T1 extraction buffer (Nucleo-Spin Tissue, Macherey-Nagel), and stored at −80°C until DNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Macherey-Nagel #740579) and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075).
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bioRxiv - Microbiology 2022Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Macherey-Nagel #740579) and were transfected into early passage NIH-3T3 cells in 6 well plates using TransIT-X2® dynamic delivery transfection system (Mirus) ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from 1 ml of crude homogenate containing 6 μl of proteinase K (22 mg/ml, Macherey-Nagel), incubated overnight at 55°C.
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bioRxiv - Biochemistry 2022Quote: ... VP Nucleodur 100-5C 18 ec column (250 ×40 mm, 7 μm: Macherey-Nagel) used as stationary phase ...
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bioRxiv - Biochemistry 2021Quote: ... with a VP 250/10 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel, Düren, Germany) running a general gradient of 10% to 70% acetonitrile (ACN ...
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bioRxiv - Plant Biology 2022Quote: ... with the difference that a Nucleodur 100-5 NH2 125x4 mm column (Macherey-Nagel, #760730.40) and an eluent consisting of 8.5% acetonitrile and 25mM formic acid pH 4 were used (18) ...
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bioRxiv - Biochemistry 2021Quote: ... The Ni2+-NTA column (4 ml; Macherey-Nagel, Düren) was equilibrated with ten column volumes of the solubilization buffer before loading the sample ...
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bioRxiv - Developmental Biology 2023Quote: ... permeabilized with 10 µg/mL Proteinase K (Macherey-Nagel) in PBS containing 0.1% tween (PBST ...
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bioRxiv - Microbiology 2019Quote: ... Total nucleic acids were extracted from mouse samples submitted to IDEXX BioAnalytics using a commercially available platform (NucleoMag® VET Kit; Macherey-Nagel GmbH & Co. KG, Düren, Germany).
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bioRxiv - Plant Biology 2021Quote: ... a Nucleoshell RP 18 plus reversed-phase column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 250 μg/mL of RNase A (Macherey-Nagel) and 0.5 mg/mL of lysing enzymes from Trichoderma harzianum (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5% β-mercaptoethanol and 10 μg/mL RNaseA (Macherey-Nagel)) and incubated for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5% β-mercaptoethanol and 10 μg/mL RNaseA (Macherey-Nagel)) and incubated for 1 hr at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 7.5% β-mercaptoethanol and 10 μg/ml RNaseA (Macherey-Nagel). The plugs were then incubated overnight at 50°C in a solution containing 250 mM EDTA (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from 100 μL blood using the Genomic DNA Extraction kit (Macherey-Nagel, Düren, Germany) and eluted in an equivalent volume of elution buffer ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... The concentrate was applied to a C18 column (Polygoprep C18, 25–40 μm, 100 Å (Macherey-Nagel), 40 i.d ...
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bioRxiv - Microbiology 2023Quote: Separation was performed on an EC 100/2 Nucleoshell Phenyl-Hexyl column (2C×C100Cmm, 2.7Cμm; Macherey-Nagel) at 40°C ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using 100 µL of homogenate with the Genomic DNA tissue kit (Macherey-Nagel, Germany). Detection and quantification of R ...