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Citations for Macherey-Nagel GmbH :
1 - 50 of 180 citations for 7 Quinolinamine 1 2 3 4 tetrahydro 1 methyl hydrochloride 1 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The resulting bottom organic phase was dried and on-line derivatized to fatty acid methyl ester by a chloroform/methanol (1:2)-TMSH solution (Macherey-Nagel) and analyzed by gas chromatography-mass spectrometry (Agilent 5977A-7890B) ...
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bioRxiv - Microbiology 2023Quote: ... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
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bioRxiv - Plant Biology 2023Quote: ... from 2 to 3-day-old WT and mea-1-/-;dme-2-/- seedlings using NucleoSpin® Plant II (MACHEREY-NAGEL). 200 ng of DNA was subjected to fragmentation using ultrasonicator (Covaris ...
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bioRxiv - Bioengineering 2022Quote: ... The remaining cuticular wax-chloroform mixture was filtered (cellulose folded filter paper; 4-12 µm pore size, M&N 615 1/4, Ø 185 mm, Macherey-Nagel GmbH & Co. KG), and chloroform was evaporated (rotary evaporator ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:100 RNase A (MACHEREY-NAGEL, 740505) was added in each sample for 15 min at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected onto an RP18 column (4 × 50 mm, 2.7 μm particle size, Nucleoshell, Macherey-Nagel) with a flow rate of 0.25 mL min−1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Harvested bacteria were lysed using a French press (3 runs at 20.000 psi) in 1× LEW buffer (Macherey-Nagel, Düren, Germany) containing 5% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was separated from the debris by centrifugation at 4000g for 40 minutes at 4°C and the supernatant was purified using 1 ml Ni-NTA columns (Macherey-Nagel). His-tagged UL12 was eluted using lysis buffer with increasing concentration of Imidazole (100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... After cell lysis using a French press the cell debris was removed by centrifugation at 17000 g for 1 h at 4 °C and the cleared lysate was incubated with Protino IDA resin (Macherey-Nagel) for 20 min at 4° C ...
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bioRxiv - Microbiology 2020Quote: ... The lysed culture was centrifuged at 46,000 x g for 1 hour and the supernatant was loaded onto a 1-ml Protino® Ni-NTA column (Macherey-Nagel). The protein was eluted with buffer A + 300 mM imidazole and protein-containing fractions were pooled and dialyzed overnight in buffer A with TEV protease (1/10 w/w ratio ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 100 μL of 1:1 diluted apical wash using the NucleoMag VET (Macherey-Nagel AG, Oensingen, Switzerland), according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
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bioRxiv - Neuroscience 2021Quote: ... 3 mice at each time point (ZT 1, 5, 9, 13, 17, 21) were sacrificed and RNA was extracted (Macherey-Nagel, Oensingen, Switzerland) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from Kasumi-1 cells after 2 days after shRUNX1::ETO knockdown was induced with doxycycline using the Nucleospin RNA kit (Macherey-Nagel). cDNA was synthesised using Superscript II (Invitrogen ...
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The complete mitochondrial genome of Spurilla braziliana MacFarland 1909 (Nudibranchia, Aeolidiidae)bioRxiv - Genomics 2023Quote: ... Japan (Figure 1) and preserved in NucleoProtect RNA (MACHEREY-NAGEL) for 5.5 months at -80 °C before DNA extraction ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared from cell debris by centrifugation at 35.000 g for 30 min and incubated for 1 hour with 2 g Ni-IDA matrix (Protino; Macherey-Nagel, Düren, Germany). The matrix was washed extensively with buffer L and bound protein was eluted in buffer L containing 250 mM imidazole ...
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bioRxiv - Genetics 2023Quote: ... and then incubated for 2 h on ice on a rocking platform with 1 ml Protino Ni-NTA Agarose (MN, Macherey-Nagel GmbH, Düren, Germany) pre-equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was loaded onto 1 ml Ni-NTA agarose beads (Macherey-Nagel), pre-equilibrated with 10 ml wash buffer and rotated end-over-end for 1h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 mM NaCl and 1 mg/ml proteinase K (Macherey-Nagel, 740506) and 0.05 mg/ml RNase A (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... and N-Methyl-N-(trimethylsilyl)trifluoroacetamide (Macherey-Nagel). Fatty acid methyl esters (C8-C24 ...
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bioRxiv - Genetics 2019Quote: ... The frozen pellet was resuspended in 1 ml of RA1 buffer (Macherey-Nagel) supplemented 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Genomics 2023Quote: ... The ground tissues were lysed with 1 ml CF lysis buffer (MACHEREY-NAGEL) supplemented with 20 μl RNase and incubated for 1 h at 65°C under agitation ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Solid-phase extraction cartridges (Chromabond HR-X, 30mg, 1 ml, Macherey-Nagel, Dueren, Germany) were conditioned with 1 ml MeOH followed by 1 ml distilled HPLC-water ...
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bioRxiv - Plant Biology 2020Quote: ... The pellet was resuspended on ice in 1 ml NucleoZol (Macherey-Nagel, Düren, Germany) and incubated for 10 minutes at 65°C with shaking at 250 rpm ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from 1 × 106 cells using NucleoSpin DNA RapidLyse (MACHEREY-NAGEL). qPCR was performed with primers targeting the ND2 locus in the mitochondrial genome (5′-TGTTGGTTATACCCTTCCCGTACTA-3′ and 5′- CCTGCAAAGATGGTAGAGTAGATGA-3′ ...
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bioRxiv - Microbiology 2023Quote: ... 29-18-1 using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Germany) with a guard column (EC 4/3 NUCLEODUR C18 Gravity, 3 μm, Macherey-Nagel GmbH & Co. KG Germany), while the column temperature was kept at 40 °C in a thermostatic column compartment (Thermo Fisher UltiMate 3000) ...
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bioRxiv - Immunology 2021Quote: ... 90 μl N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA; Macherey-Nagel) containing 0.2 μg/ ml each of C4-C24 fatty acid methyl esters (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... 80 μL N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA, Macherey-Nagel) was added and the samples were incubated at 37 °C for 30 min (1.200 rpm) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 80 µL of N-methyl-N-trimethylsilyl-trifluoroacetamid (Macherey-Nagel) were added ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from 1 mL overnight culture using NucleoSpin Plasmid Easypure columns (Macherey-Nagel) from the Plasmid Easypure Kit (Nucleospin).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with preequilibrated Protino Ni-NTA beads (1 ml resin, Macherey-Nagel) at 4°C for 1h ...
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bioRxiv - Microbiology 2023Quote: ... PA14 and PAK from ∼1 × 108 CFU using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel), as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... in pyridine followed by a 30 min treatment at 37 °C with 100 μI of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). For each sample ...
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bioRxiv - Plant Biology 2022Quote: ... in pyridine followed by a 30 min treatment at 37 °C with 70 μl of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). An autosampler Gerstel Multi-Purpose system (Gerstel GmbH & Co.KG ...
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bioRxiv - Plant Biology 2023Quote: ... in pyridine followed by a 30 min treatment at 37°C with 70 μl of N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). An autosampler Gerstel Multi-Purpose system (Gerstel GmbH & Co ...
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bioRxiv - Neuroscience 2021Quote: ... After adding 30 µl N-methyl-N-trimethylsilyl-trifluoroacetamide (Macherey-Nagel), samples were incubated for 30 min at 45 °C under continuous shaking.
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bioRxiv - Cell Biology 2023Quote: ... After adding 30 µl N-methyl-N-trimethylsilyl-trifluoroacetamide (Macherey-Nagel), samples were incubated for 30 min at 45°C under continuous shaking.
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bioRxiv - Cell Biology 2019Quote: ... 1 ml supernatant containing Upa2 variant was added directly to the washed Ni-NTA (Macherey-Nagel) bead ...
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bioRxiv - Evolutionary Biology 2022Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Gibson assembly was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... Pre_S or FECD corneal endothelial tissues (Supplemental Table 1) by NucleoSpin RNA XS kit (Macherey-Nagel). cDNAs were prepared by reverse transcription ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM PMSF) by sonication followed by loading on to Ni-IDA column (Macherey-Nagel, Germany). Endonuclease G was eluted in gradient imidazole concentration (100-500 mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was first extracted from 1 x 105 cells using NucleoSpin Tissue (MACHEREY-NAGEL #740952) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was then loaded on a Protino® GST/4B column 1 ml (Macherey-Nagel) for pull-down experiments ...
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bioRxiv - Systems Biology 2019Quote: ... RNA was recovered from 1 x 106 cells using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The RNA was sent to Eurofins for RNA-seq analysis ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µl from each sample was spotted onto a POLYGRAM CEL 300 PEI-TLC plate (Macherey-Nagel), and developed with 0.5 M LiCl (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... was purified from a 1% agarose gel using a Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The PCR product (325 bp) was purified from a 1% agarose gel via NucleoSpin columns (Macherey-Nagel). NGS was performed on an Illumina Miniseq using a MiniSeq Mid Output Kit (300-cycles ...
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bioRxiv - Physiology 2019Quote: ... 50 μL of N-Methyl-N-(trimethylsilyl) trifluoroacetamide (Macherey-Nagel, Düren, Germany) was added to each sample and incubated at 40 °C for 1 h ...