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Citations for Macherey-Nagel GmbH :
101 - 133 of 133 citations for 7 Benzyl 2 oxa 7 aza spiro4.4nonan 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... In the following steps each amplicon generated either by PCR amplification or vector digestion was isolated and extracted from a 1% agarose gel through the NucleoSpin Gel (Macherey-Nagel) and PCR Clean-Up Kit (Takara ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA from each sample was isolated using the NucleoSpin® RNA isolation kit (Macherey-Nagel, Hoerdt Cedex, France) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Clones whose culture had proven successful (IgG concentration ≥ 1 μg/mL at day 21-25) were selected and extracted using the NucleoSpin96 RNA extraction kit (Macherey-Nagel) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Both PCR products were excised from a 1% agarose gel and extracted using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) and used as templates for In-Fusion HD cloning (Takara) ...
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bioRxiv - Genomics 2021Quote: ... Resulting amplicons were loaded on a 1% agarose gel and purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Libraries were sequenced paired-end 75bp on the NextSeq 500 platform yielding approximately 4*106 fragments per sample (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2019Quote: ... The lipid was then dissolved in the 1-butanol and separated by two dimension high performance thin layer chromatography (NanoSiL, MACHEREY-NAGEL). For the first dimension ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from three independent experiments of the mock- and KSHV-infected lymphatic ECFCs isolated as an adherent subpopulation from donor 1 using the NucleoSpin RNA extraction kit (Macherey-Nagel), after which purity and concentration was determined with NanoDrop spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Barcoded amplicons were extracted from agarose gel (1.5%, 1 h, 100 V) by using NucleoSpin® Gel and PCR Cleanup XS kit (Macherey-Nagel) and the amplicon size and DNA concentration were checked with High Sensitivity D1000 ScreenTape assay in 2200 TapeStation System (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and verified by Sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and sent for Sanger sequencing.
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bioRxiv - Physiology 2023Quote: ... Products were run on 1% agarose gels and then gel-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Clones whose culture had proven successful (IgG concentration ≥ 1 μg/mL at day 21-25) were selected and extracted using the NucleoSpin96 RNA extraction kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products were visualized on a 1% TAE agarose gel and purified using the Nucleospin PCR Clean-up Kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from immature inflorescences (stages 1-12) or aerial parts of 16- day-old seedlings using NucleoSpin RNA Plus kit (Macherey-Nagel) following the manufacturer’s recommendations ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The digestion reaction was run on a 1% agarose gel followed by excision of the digested band and purification via NucleoSpin columns (Macherey-Nagel). 100 ng digested pMB1-10x and 2.4 ng amplified sgRNA library insert were assembled using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was separated from the debris by centrifugation at 4000g for 40 minutes at 4°C and the supernatant was purified using 1 ml Ni-NTA columns (Macherey-Nagel). His-tagged UL12 was eluted using lysis buffer with increasing concentration of Imidazole (100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... After cell lysis using a French press the cell debris was removed by centrifugation at 17000 g for 1 h at 4 °C and the cleared lysate was incubated with Protino IDA resin (Macherey-Nagel) for 20 min at 4° C ...
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bioRxiv - Neuroscience 2024Quote: DNA was extracted from blood samples of patients II-8 and IV-1 using the Nucleospin extraction kit (ref 740952-Macherey-Nagel). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... The His6-3C protease was then removed from the sample by immobilized metal affinity chromatography using a 1 mL Protino Ni-NTA column (Macherey-Nagel). The mTRIM66 PHD-bromo-domain protein was further purified by size exclusion chromatography using a HiLoad 16/600 Superdex 75 pg column (Cytiva ...
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bioRxiv - Genomics 2024Quote: ... dissolved in TE buffer (10 mM Tris pH8, 1 mM EDTA pH8) and purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel). DNA was loaded on a 2% agarose gel ...
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bioRxiv - Plant Biology 2021Quote: ... The aqueous phase was transferred to a new tube and incubated with 1 μL of RNase A (Macherey-Nagel, 12mg/mL stock) for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: Cells were lysed (RLT buffer with 1% β-mercaptoethanol) and RNA was isolated via column purification (NucleoSpin RNA kit, Macherey-Nagel 740955). RNA integrity and concentration were determined with BioAnalyzer (Agilent ...
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bioRxiv - Cell Biology 2023Quote: The final PCR amplified library was submitted to two consecutive 1× NucleoMag NGS Clean-up and Size select magnetic bead selections (Macherey-Nagel – 7449970.5) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from INS-1 832/13 cells 48h after transfection using the NucleoSpin extraction kit (Macherey-Nagel, Düren, Germany). The amount of isolated RNA was measured using the NanoDrop system ...
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bioRxiv - Neuroscience 2021Quote: ... 3 mice at each time point (ZT 1, 5, 9, 13, 17, 21) were sacrificed and RNA was extracted (Macherey-Nagel, Oensingen, Switzerland) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: OMM bacteria were grown as described and 1 mL of each bacterial culture supernatant was homogenized using NucleoSpin Bead Tubes (Macherey-Nagel, Düren, Germany) and a Precellys Evolution Homogenizer (Bertin Corp. ...
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bioRxiv - Bioengineering 2022Quote: Cultured cells were lysed in Macherey-Nagel RA1 buffer containing 1% β-mercaptoethanol and total RNAs were isolated using NucleoSpin RNA isolation kit (Macherey-Nagel 740955). Reverse transcription and PCR amplification were performed as described before21 ...
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bioRxiv - Microbiology 2020Quote: ... The final PCR product from the 5’ RACE procedure was excised from a 1% agarose gel and purified by column capture (Macherey-Nagel Nucleospin mini kit). The purified product was then Sanger sequenced (Eurofins Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we measured the chemical oxygen demand (COD) using NANOCOLOR COD tube tests (detection range 1-15 g/l by Macherey-Nagel (ref: 985 038), see (32 ...
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bioRxiv - Bioengineering 2022Quote: ... The remaining cuticular wax-chloroform mixture was filtered (cellulose folded filter paper; 4-12 µm pore size, M&N 615 1/4, Ø 185 mm, Macherey-Nagel GmbH & Co. KG), and chloroform was evaporated (rotary evaporator ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These were obtained by using as template a 1:10 serial dilution (8 dilutions) of a cleaned (Macherey-Nagel’s NucleoSpin Gel and PCR Clean-up) and quantified (Invitrogen’s Qubit™ ...
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bioRxiv - Genomics 2024Quote: ... each side of the Amicon filter was rinsed and incubated at 56°C for 1 hour (per side) in 200 µL Buffer T1 and 20 µL Proteinase K from the NucleoSpinl1J Tissue Kit (MACHEREY-NAGEL Inc., Allentown, PA, United States) and DNA extraction proceeded from step 3 of the kit ...