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Citations for Macherey-Nagel GmbH :
1 - 50 of 131 citations for 7 Azabicyclo 2.2.1 heptane 7 carboxylic acid 2 phenylmethoxy carbonyl amino 1 1 dimethylethyl ester 1R 2S 4S rel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The resulting bottom organic phase was dried and on-line derivatized to fatty acid methyl ester by a chloroform/methanol (1:2)-TMSH solution (Macherey-Nagel) and analyzed by gas chromatography-mass spectrometry (Agilent 5977A-7890B) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2022Quote: ... VP Nucleodur 100-5C 18 ec column (250 ×40 mm, 7 μm: Macherey-Nagel) used as stationary phase ...
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bioRxiv - Microbiology 2023Quote: ... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
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bioRxiv - Microbiology 2020Quote: ... the soluble fraction was directly loaded onto a 1-ml nickel column (Protino Ni-nitrotriacetic acid [NTA]; Macherey-Nagel). The column was washed with Wash Buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... from 2 to 3-day-old WT and mea-1-/-;dme-2-/- seedlings using NucleoSpin® Plant II (MACHEREY-NAGEL). 200 ng of DNA was subjected to fragmentation using ultrasonicator (Covaris ...
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bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from Kasumi-1 cells after 2 days after shRUNX1::ETO knockdown was induced with doxycycline using the Nucleospin RNA kit (Macherey-Nagel). cDNA was synthesised using Superscript II (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared from cell debris by centrifugation at 35.000 g for 30 min and incubated for 1 hour with 2 g Ni-IDA matrix (Protino; Macherey-Nagel, Düren, Germany). The matrix was washed extensively with buffer L and bound protein was eluted in buffer L containing 250 mM imidazole ...
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bioRxiv - Genetics 2023Quote: ... and then incubated for 2 h on ice on a rocking platform with 1 ml Protino Ni-NTA Agarose (MN, Macherey-Nagel GmbH, Düren, Germany) pre-equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:100 RNase A (MACHEREY-NAGEL, 740505) was added in each sample for 15 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The lysed culture was centrifuged at 46,000 x g for 1 hour and the supernatant was loaded onto a 1-ml Protino® Ni-NTA column (Macherey-Nagel). The protein was eluted with buffer A + 300 mM imidazole and protein-containing fractions were pooled and dialyzed overnight in buffer A with TEV protease (1/10 w/w ratio ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 100 μL of 1:1 diluted apical wash using the NucleoMag VET (Macherey-Nagel AG, Oensingen, Switzerland), according to the manufacturer’s guidelines ...
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The complete mitochondrial genome of Spurilla braziliana MacFarland 1909 (Nudibranchia, Aeolidiidae)bioRxiv - Genomics 2023Quote: ... Japan (Figure 1) and preserved in NucleoProtect RNA (MACHEREY-NAGEL) for 5.5 months at -80 °C before DNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted using the pathogen Nucleic Acid Extraction kit (Macherey-Nagel, Düren, Germany) and eluted in 50 μL elution buffer ...
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bioRxiv - Plant Biology 2021Quote: ... an EC 100/2 Nucleoshell Phenyl-Hexyl column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was loaded onto 1 ml Ni-NTA agarose beads (Macherey-Nagel), pre-equilibrated with 10 ml wash buffer and rotated end-over-end for 1h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 mM NaCl and 1 mg/ml proteinase K (Macherey-Nagel, 740506) and 0.05 mg/ml RNase A (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Genetics 2019Quote: ... The frozen pellet was resuspended in 1 ml of RA1 buffer (Macherey-Nagel) supplemented 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Genomics 2023Quote: ... The ground tissues were lysed with 1 ml CF lysis buffer (MACHEREY-NAGEL) supplemented with 20 μl RNase and incubated for 1 h at 65°C under agitation ...
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bioRxiv - Genetics 2021Quote: ... Nucleic acid extraction was performed with the NucleoSpin DNA Insect Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Solid-phase extraction cartridges (Chromabond HR-X, 30mg, 1 ml, Macherey-Nagel, Dueren, Germany) were conditioned with 1 ml MeOH followed by 1 ml distilled HPLC-water ...
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bioRxiv - Plant Biology 2020Quote: ... The pellet was resuspended on ice in 1 ml NucleoZol (Macherey-Nagel, Düren, Germany) and incubated for 10 minutes at 65°C with shaking at 250 rpm ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from 1 × 106 cells using NucleoSpin DNA RapidLyse (MACHEREY-NAGEL). qPCR was performed with primers targeting the ND2 locus in the mitochondrial genome (5′-TGTTGGTTATACCCTTCCCGTACTA-3′ and 5′- CCTGCAAAGATGGTAGAGTAGATGA-3′ ...
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bioRxiv - Microbiology 2023Quote: ... 29-18-1 using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from ethylenediaminetetraacetic acid (EDTA)-anticoagulated blood using NucleoSpin Blood Quick Pure kit (Macherey-Nagel, Duren, Germany) and was stored in the gene bank run by Azabu University (Kanagawa ...
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bioRxiv - Microbiology 2023Quote: The nucleic acids were extracted from the FFPE tissue sections using the Nucleospin total RNA FFPE XS (Macherey-Nagel). RNA sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... The sample was then acidified using trifluoroacetic acid and peptides were purified using Chromabond C18 Microspin columns (Macherey-Nagel).
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bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from 1 mL overnight culture using NucleoSpin Plasmid Easypure columns (Macherey-Nagel) from the Plasmid Easypure Kit (Nucleospin).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with preequilibrated Protino Ni-NTA beads (1 ml resin, Macherey-Nagel) at 4°C for 1h ...
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bioRxiv - Microbiology 2023Quote: ... PA14 and PAK from ∼1 × 108 CFU using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel), as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Amino acids were separated on a reverse-phase C18 column (EC 250/2 NUCLEOSIL 100-3 C18HD, L=250 mm, ID: 2 mm; Macherey-Nagel). Samples (5 µL ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μL sample volume was injected into a Nucleodur HILIC column (250 mm * 2 mm, 3 μm particle size, Macherey-Nagel). Metabolites were eluted at 20 °C with an acetonitrile gradient (90% (v/v ...
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bioRxiv - Synthetic Biology 2022Quote: ... SLS was precipitated with trifluoroacetic acid (TFA, 1.5% final concentration) and peptides were purified using Chromabond C18 microspin columns (Macherey-Nagel). Acidified peptides were loaded on spin columns equilibrated with 400 μL acetonitrile and then 400 μL 0.15% TFA ...
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bioRxiv - Plant Biology 2020Quote: ... LMW carbonyls were separated using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an Ekspert ultraLC 100 UHPLC system (AB SCIEX ...
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bioRxiv - Plant Biology 2023Quote: ... Separation was carried out using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an ekspert ultraLC 100 UHPLC system coupled to a QTRAP 4500 mass spectrometer (AB SCIEX ...
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bioRxiv - Cell Biology 2019Quote: ... 1 ml supernatant containing Upa2 variant was added directly to the washed Ni-NTA (Macherey-Nagel) bead ...
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bioRxiv - Evolutionary Biology 2022Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Gibson assembly was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... Pre_S or FECD corneal endothelial tissues (Supplemental Table 1) by NucleoSpin RNA XS kit (Macherey-Nagel). cDNAs were prepared by reverse transcription ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM PMSF) by sonication followed by loading on to Ni-IDA column (Macherey-Nagel, Germany). Endonuclease G was eluted in gradient imidazole concentration (100-500 mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was first extracted from 1 x 105 cells using NucleoSpin Tissue (MACHEREY-NAGEL #740952) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: Ribonucleic acid (RNA) samples were isolated from seeds and leaves using the NucleoSpin® RNA Plant kit (Macherey-Nagel, Düren, Germany) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was then loaded on a Protino® GST/4B column 1 ml (Macherey-Nagel) for pull-down experiments ...
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bioRxiv - Systems Biology 2019Quote: ... RNA was recovered from 1 x 106 cells using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The RNA was sent to Eurofins for RNA-seq analysis ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µl from each sample was spotted onto a POLYGRAM CEL 300 PEI-TLC plate (Macherey-Nagel), and developed with 0.5 M LiCl (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... was purified from a 1% agarose gel using a Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The PCR product (325 bp) was purified from a 1% agarose gel via NucleoSpin columns (Macherey-Nagel). NGS was performed on an Illumina Miniseq using a MiniSeq Mid Output Kit (300-cycles ...
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bioRxiv - Evolutionary Biology 2020Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean‑up (Macherey-Nagel, Düren, Germany). Following digestion with restriction enzymes ...