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Citations for Macherey-Nagel GmbH :
51 - 100 of 167 citations for 7 8 9 10 TETRAHYDRO 6H AZEPINO 2 1 B QUINAZOLIN 12 YLIDENEAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... a Nucleoshell RP 18 plus reversed-phase column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Genomics 2020Quote: Total mRNA of edited HUDEP-2 cells was isolated using the NucleoSpin RNA kit (Macherey-Nagel) and reverse transcribed into cDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... The supernatant was collected and loaded onto 2 mL pre-equilibrated Ni-NTA agarose (Macherey-Nagel). The mixture was rotated at 4 □ for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... elegans animals were transferred to 2 ml MN Bead Tubes Type A (Macherey-Nagel, Düren, Germany) and lysed using a Precellys Bead Beating system with an additional Cryolys cooling module (Bertin Instruments ...
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bioRxiv - Cancer Biology 2023Quote: ... BM DOHH2 were resuspended in RA1 buffer and tris(2-carboxyethyl)phosphine (TCEP) from Macherey-Nagel and stored at -80°C for less than 3 months ...
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bioRxiv - Biochemistry 2021Quote: ... with a VP 250/10 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel, Düren, Germany) running a general gradient of 10% to 70% acetonitrile (ACN ...
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bioRxiv - Microbiology 2022Quote: The yeast expression vector pAG416-GAL-SARS-CoV-2-3CL was freshly prepared by Midiprep (Macherey-Nagel), and variants were introduced by a modified single primer site-directed mutagenesis method (Shenoy and Visweswariah ...
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bioRxiv - Microbiology 2023Quote: Separation was performed on an EC 100/2 Nucleoshell Phenyl-Hexyl column (2C×C100Cmm, 2.7Cμm; Macherey-Nagel) at 40°C ...
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bioRxiv - Biochemistry 2023Quote: ... SUV420H1 proteins were isolated by incubating the soluble fraction for 2 hrs with GST beads (Macherey-Nagel). The beads were washed with two column volumes of wash buffer (20 mM Tris ...
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bioRxiv - Genomics 2023Quote: ... DNA purification was carried out by adding a 2 ml mixture of wash buffer PW2 (MACHEREY-NAGEL), wash buffer B5 (MACHEREY-NAGEL) ...
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bioRxiv - Biochemistry 2019Quote: ... For preparative HPLC applications a VP 250/32 Nucleodur C18 HTec 10 μm column (Macherey-Nagel) was used ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from isolated islets from 2 mice per genotype with Nucleospin RNA plus XS (Macherey-Nagel). Complementary DNA was generated and linearly amplified from 3 ng total RNA using the Ovation RNA-seq V2 system (NuGEN technologies Inc. ...
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bioRxiv - Genetics 2020Quote: ... Samples were separated on a C18 column (EC 100/2 Nucleoshell RP-18, 2.7 μm, from Macherey-Nagel) using a gradient of 0.1% formic acid / isopropanol over 0.1% formic acid / water ...
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bioRxiv - Molecular Biology 2023Quote: ... the soluble fraction was incubated for 2 hours in batch with Ni2+-nitrilotriacetate (NTA) agarose resin (Macherey-Nagel). After extensive washing of the beads with lysis buffer ...
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bioRxiv - Synthetic Biology 2022Quote: ... gDNA was extracted from 10 individuals from pools bgcn-Cas9D using the NucleoSpin Tissue kit (Macherey-Nagel). DNA was digested with the restriction enzymes BamHI ...
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bioRxiv - Plant Biology 2023Quote: ... then added 1.2 µL of RNase (MN, Macherey-Nagel, Ref. 740505, 1200 U/mL, 10 mg/mL), and transferred the DNA to a 96-well KingFisher Flex plate (ThermoFischer Scientific ...
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bioRxiv - Pathology 2022Quote: We extracted total RNA from 10 mg liver tissue using NucleoZOL reagent (Macherey-Nagel GmbH & Co. KG, Germany). First ...
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bioRxiv - Neuroscience 2021Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected by gel electrophoresis on 2% Agarose/TAE and gel extracted by column purification (Macherey-Nagel). Amplicons were paired-end sequenced with 500 cycles on a MiSeq system (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected for 400-550 bp using a 2% agarose/TAE gel and column purification (Macherey-Nagel). Libraries were sequenced as above ...
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bioRxiv - Genomics 2022Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from 10 ml of culture using the NucleoSpin RNA kit (Macherey-Nagel Inc., PA, USA) as described by the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... genomic DNA was isolated from a confluent 3.5- or 10-cm dish with the Nucleospin Tissue Kit (Macherey-Nagel). The rearranged region between the promoter and GOIs (500-600 bp ...
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bioRxiv - Plant Biology 2022Quote: ... for 10 min at 37°C and then cleaned up with the NucleoSpin RNA clean-up kit (Macherey-Nagel). RNA quality was verified on a 2100 Bioanalyzer Instrument (Agilent ...
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA from a male wild type blood sample (2 ml) was purified with the NucleoBand®HMW DNA Kit (Macherey-Nagel). To eliminate fragments below 40 kb the Short Read Elimination Kit XL (Circulomics ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were excised from 2% agarose gels and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) using NTC buffer for solubilization of the gel slices ...
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bioRxiv - Neuroscience 2021Quote: Viral RNA was isolated from 4 µl of a typical lentivirus preparation (∼2×106 TU/µl) using the NucleoSpin RNA Virus mini kit (Macherey-Nagel). Purified RNA was used as a template for reverse transcription using the SuperScript VILO cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Solid phase extraction was carried out using Chromabond SiOH cartridges filled with 2 g of unmodified silica gel (Macherey-Nagel, Germany). Chemicals ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PCR products were confirmed on 2% agarose gels and purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Purified PCR products were quantified using the Quant-iT dsDNA kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Total RNA was prepared from isolated colonic epithelial cells of Hsp60Δ/ΔIEC and Hsp60fl/fl mice on day 2 with the column-based NucleoSpin RNAII kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Virus was purified by centrifugation at 12,000 xg for 10 mins at 4 °C and genomic DNA extracted (NucleoSpin Tissue kit, Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were centrifuged at 21000 g for 10 min and supernatants were transferred to silica membrane spin columns (Macherey-Nagel) and centrifuged at 11000 g for 1 min ...
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bioRxiv - Biochemistry 2023Quote: ... of a saline solution (0.9%) and immediately stored at −20°C in 10 vol NucleoProtect RNA stabilization reagent (Macherey-Nagel) before further RNA extraction and target mRNA quantification.
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bioRxiv - Molecular Biology 2024Quote: ... Chromatography of 10 µL of each sample was performed using a Nucleoshell C18 reversed phase column (Macherey-Nagel, Düren, Germany) for human bile acids ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA genes of AOB and AOA was amplified with primers amoA-1F/amoA-2R (66 ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from 2 × 105 cells untreated or butyrate-conditioned BMDMs using the NucleoSpin RNA extraction kit (Macherey-Nagel, 740902.50) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... and 6 µg total endotoxin free plasmid DNA (4 µg HaloTag-KIF5B1-560 plasmid DNA + 2 µg of α-tubulin-GFP) isolated using the NucleoBond Xtra Midi Plus EF kit (MACHEREY-NAGEL GmbH & Co ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products from the same sample were pooled and purified over 2% agarose gels (band size at 275 bp) using NucleoSpin® Gel and PCR clean up kit (Macherey-Nagel). Purified amplicons were quantified by Qubit (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA gene of Ca ...
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bioRxiv - Biochemistry 2020Quote: ... 4°C for 45 min) and the supernatant was incubated for 25 min at 4°C with 2 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Neuroscience 2020Quote: ... The total proteins were precipitated and prepared for Polyacrylamide Gel Electrophoresis using protein precipitator and resuspension buffer PSB/TCEP (Protein solving buffer and (tris(2-carboxyethyl)phosphine) TCEP reducing agent) from the NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France). The elution fraction containing RNA was further used for PCR analysis (see below) ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µL of extracts were injected into a Nucleoshell RP 18 column (2.7 µm x 150 mm x 2 mm, Macherey-Nagel GmbH, Düren, Germany). A solvent system composed of A ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:100 RNase A (MACHEREY-NAGEL, 740505) was added in each sample for 15 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a 10% SDS-polyacrylamide gels and transferred onto a 0.45 µm pore size nitrocellulose membrane (Macherey-Nagel GMBH: 741280) by wet blotting ...
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bioRxiv - Evolutionary Biology 2021Quote: ... equipped with a photodiode array detector and a semi-preparative RP-Nucleodur C18 Htec column (5 µm, 250 × 10 mm, Macherey-Nagel, Düren, Germany) using a water-acetonitrile gradient ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... containing 10 nM internal standard, and 100 µL were transferred into mass spectrometry vials (Wicom, Heppenheim, Germany) with inserts (Macherey-Nagel, Düren, Germany) for carprofen quantification ...
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bioRxiv - Microbiology 2020Quote: ... The lysed culture was centrifuged at 46,000 x g for 1 hour and the supernatant was loaded onto a 1-ml Protino® Ni-NTA column (Macherey-Nagel). The protein was eluted with buffer A + 300 mM imidazole and protein-containing fractions were pooled and dialyzed overnight in buffer A with TEV protease (1/10 w/w ratio ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 100 μL of 1:1 diluted apical wash using the NucleoMag VET (Macherey-Nagel AG, Oensingen, Switzerland), according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2021Quote: ... Both peptide segments were purified by C18 reverse phase (RP)-HPLC on a Nucleosil C18 column (300 Å, 250 × 10 mm) (Macherey-Nagel, Düren, Germany), maintained at 25°C ...