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Citations for Macherey-Nagel GmbH :
151 - 200 of 231 citations for 7 7 4 4 Bipiperidine 1 1 diyldi 2 1 ethanediyl bis 10 methoxy 7H pyrido 4 3 c carbazole tetramethanesulfonate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ACE and its metabolites ANSA and acetoacetate were quantified by HPLC with an UV/VIS detector employing a Nucleosil 100-5 C18 HD column (240/3 mm, Macherey-Nagel) and an eluent at 0.5 ml min−1 containing 100 mM NaH2PO4 and 10 mM tetrabutylammoniumhydrogensulfate ...
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bioRxiv - Genomics 2023Quote: The genomic DNA was extracted from at least one million cells (typically 3 million, coverage 300x) using the NucleoSpin Tissue kit (Macherey-Nagel). To enrich for GBCs ...
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bioRxiv - Microbiology 2023Quote: ... and 300 mg (JFC) of both fresh and treated frass (n = 3) using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) and following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm×3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel), at 40°C ...
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bioRxiv - Plant Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm × 3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel), at 40 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900 rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... for 30 min at 37 °C and gel purified with a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). The oligo-pool amplicons were assembled into the linearized Lenti-gRNA-Puro plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... Input and ChIP sample crosslinking were reversed at 65°C overnight in 50 μl TE + 200 mM NaCl + 0.1% SDS + 20-40 μg PK (Macherey-Nagel 740506). For sequencing ...
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bioRxiv - Microbiology 2021Quote: ... 107 cells were inoculated in 50 ml LB medium supplemented with 100 µg/ml spectinomycin and grown overnight at 37 °C for purification of the plasmid library DNA by miniprep (NucleoSpin Plasmid, Macherey-Nagel). The efficiency of fragment insertion (100% ...
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bioRxiv - Microbiology 2021Quote: ... selected clones were grown overnight at 37°C under agitation and the plasmid DNA was extracted and purified using the NucleoSpin plasmid Mini or Midi kit (Macherey-Nagel).
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bioRxiv - Genomics 2021Quote: ... genomic DNA was extracted from bacterial strains grown on King’s medium B (King et al. 1954) at 28°C for 24 h using NucleoSpin Microbial DNA kit (Macherey-Nagel, Germany). The quality of the genomic DNA was assessed by electrophoresis in 0.8% agarose gel.
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bioRxiv - Genomics 2019Quote: ... One colony per construct was grown overnight in a shake incubator at 37°C before plasmid extraction using the NucleoSpin Plasmid Transfection-grade kit (Macherey-Nagel). For each construct three biological replicates were performed by transfecting the plasmids into 80% confluent cells of MM001 in a 24 well plate ...
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bioRxiv - Biochemistry 2022Quote: Total RNAs were extracted from mid-log yeast cultures grown 16 h at 20°C in YPD as described above using the Nucleospin RNA II kit (Macherey-Nagel) and were reverse transcribed with Superscript-II reverse transcriptase (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel). The selection markers for fungal transformation were cloned into digested plasmids by using the Rapid DNA Ligation Kit (Thermo) ...
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bioRxiv - Plant Biology 2022Quote: ... The eluted desulfoglucosinolates were separated on a reversed phase C-18 column (Nucleodur Sphinx RP, 250 x 4.6 mm, 5µm, Macherey-Nagel, Düren, Germany) with a water (A)-acetonitrile (B ...
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bioRxiv - Molecular Biology 2023Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... All samples were immediately placed on dry ice and stored at −80 °C until DNA extraction using the NucleoSpin 96 soil kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... the DNA pellet was dried in the oven at 70°C for 30 min then re-suspended in 100 μl elution buffer BE (MACHEREY-NAGEL) (5 mM Tris solution ...
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bioRxiv - Genomics 2024Quote: ... Cross-linking was reversed by overnight incubation at 65°C and DNA fragments were purified using NucleoSpin Gel and PCR Clean-up columns (Macherey-Nagel).
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids were propagated in cultures of terrific broth medium with appropriate antibiotics overnight at 37 °C and purified with NucleoSpin miniprep kit (Macherey-Nagel) following the manufacturer’s prototol ...
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bioRxiv - Microbiology 2023Quote: ... and DMSO as a control at 28°C and 150 rpm using a NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... equipped with a flame ionization detector (FID) and an Optima-s-3 GC capillary column (30 m x 0.25 mm x 0.25 µm - Macherey-Nagel GmbH & Co, Germany). Nitrogen (200 kPa ...
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bioRxiv - Evolutionary Biology 2020Quote: Fifteen microliters of extract were injected into an Agilent 1100 series HPLC and compounds were separated on an EC 150/4.6 NUCLEODUR® C18 Gravity column (3 μm, 150 mm x 4.6 mm, Macherey-Nagel, Düren, Germany). Cardenolides and bufadienolides were eluted at a constant flow of 0.7 ml/min at 30°C with an acetonitrile–H2O gradient as follows ...
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bioRxiv - Plant Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm×3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel, Germany), at 40°C ...
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bioRxiv - Biochemistry 2020Quote: ... using a Nucleodur HTec C18 VP 125/10 semi-preparative column (Macherey-Nagel, Germany), with an average particle diameter of 5 μm and pores 100 Å and a geometry of 150 × 3.0 mm ...
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1H NMR chemical exchange techniques reveal local and global effects of oxidized cytosine derivativesbioRxiv - Biophysics 2021Quote: ... As stationary phase Nucleodur columns (250/10 mm, C18ec, 5 μm) from Macherey-Nagel were used ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Reaction mixtures were incubated at 40°C for 4 h followed by 12 h incubation at 12°C and applied at 120 nl/s to NANO-ADAMANT HP-TLC plates (Macherey-Nagel, Germany) with a CAMAG Linomat 5 TLC sampler (CAMAG ...
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bioRxiv - Microbiology 2020Quote: We extracted DNA from the biomass pellets stored at −20°C using a NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Deutschland), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... was added for 30 min at 37°C and DNA was purified using NucleoSpin Gel and PCR Clean-Up columns (Macherey-Nagel, #740609.250) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were grown in Luria-Bertani Broth at 37°C and plasmid DNA was isolated using the Nucleobond Xtra midi kit (MACHEREY-NAGEL, 740410), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... these cultures were incubated overnight (800 RPM, 37°C) and miniprepped the following day using a 96 Plasmid NucleoSpin kit (Macherey-Nagel #740625.1). The resulting plasmids were able to be sequenced verified for proper VH and VL insertion ...
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bioRxiv - Developmental Biology 2024Quote: ... The three constructs were then cultured for 24 h at 37°C and purified with NucleoBond Xtra Midi Kit (Macherey-Nagel, Germany) and used for gene reporter assay.
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bioRxiv - Plant Biology 2021Quote: ... a Nucleoshell RP 18 plus reversed-phase column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Genomics 2020Quote: Total mRNA of edited HUDEP-2 cells was isolated using the NucleoSpin RNA kit (Macherey-Nagel) and reverse transcribed into cDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... The supernatant was collected and loaded onto 2 mL pre-equilibrated Ni-NTA agarose (Macherey-Nagel). The mixture was rotated at 4 □ for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... elegans animals were transferred to 2 ml MN Bead Tubes Type A (Macherey-Nagel, Düren, Germany) and lysed using a Precellys Bead Beating system with an additional Cryolys cooling module (Bertin Instruments ...
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bioRxiv - Cancer Biology 2023Quote: ... BM DOHH2 were resuspended in RA1 buffer and tris(2-carboxyethyl)phosphine (TCEP) from Macherey-Nagel and stored at -80°C for less than 3 months ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 ml of mutant culture from propagation round 3 were used for gDNA isolation using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). Whole genome sequencing was performed via the INVIEW Resequencing service for bacterial genomes up to 10 Mb with an Illumina standard genomic library provided by Eurofins Genomics (Ebersberg ...
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bioRxiv - Genetics 2024Quote: Total RNA from twenty heads of 3 day-old Drosophila adults for each genetic combination was purified using the RNA isolation kit of Macherey-Nagel (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions.1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the samples were frozen and stored at −80 °C until the total RNA was extracted using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany). The transcriptome was analyzed for three replicates from independent runs of the exposure experiment ...
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bioRxiv - Biochemistry 2021Quote: ... with a VP 250/10 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel, Düren, Germany) running a general gradient of 10% to 70% acetonitrile (ACN ...
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bioRxiv - Microbiology 2022Quote: The yeast expression vector pAG416-GAL-SARS-CoV-2-3CL was freshly prepared by Midiprep (Macherey-Nagel), and variants were introduced by a modified single primer site-directed mutagenesis method (Shenoy and Visweswariah ...
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bioRxiv - Microbiology 2023Quote: Separation was performed on an EC 100/2 Nucleoshell Phenyl-Hexyl column (2C×C100Cmm, 2.7Cμm; Macherey-Nagel) at 40°C ...
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bioRxiv - Biochemistry 2023Quote: ... SUV420H1 proteins were isolated by incubating the soluble fraction for 2 hrs with GST beads (Macherey-Nagel). The beads were washed with two column volumes of wash buffer (20 mM Tris ...
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bioRxiv - Genomics 2023Quote: ... DNA purification was carried out by adding a 2 ml mixture of wash buffer PW2 (MACHEREY-NAGEL), wash buffer B5 (MACHEREY-NAGEL) ...
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bioRxiv - Biophysics 2022Quote: ... and the 3’-terminal poly A) were amplified by PCR and purified using the NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2019Quote: ... For preparative HPLC applications a VP 250/32 Nucleodur C18 HTec 10 μm column (Macherey-Nagel) was used ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from isolated islets from 2 mice per genotype with Nucleospin RNA plus XS (Macherey-Nagel). Complementary DNA was generated and linearly amplified from 3 ng total RNA using the Ovation RNA-seq V2 system (NuGEN technologies Inc. ...
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bioRxiv - Genetics 2020Quote: ... Samples were separated on a C18 column (EC 100/2 Nucleoshell RP-18, 2.7 μm, from Macherey-Nagel) using a gradient of 0.1% formic acid / isopropanol over 0.1% formic acid / water ...