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Citations for Macherey-Nagel GmbH :
51 - 100 of 206 citations for 6 OXO 1 2 DIHYDRO 6H PYRROLO 3 2 1 IJ QUINOLINE 5 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from 2 × 105 cells untreated or butyrate-conditioned BMDMs using the NucleoSpin RNA extraction kit (Macherey-Nagel, 740902.50) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... and 6 µg total endotoxin free plasmid DNA (4 µg HaloTag-KIF5B1-560 plasmid DNA + 2 µg of α-tubulin-GFP) isolated using the NucleoBond Xtra Midi Plus EF kit (MACHEREY-NAGEL GmbH & Co ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products from the same sample were pooled and purified over 2% agarose gels (band size at 275 bp) using NucleoSpin® Gel and PCR clean up kit (Macherey-Nagel). Purified amplicons were quantified by Qubit (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA gene of Ca ...
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bioRxiv - Plant Biology 2023Quote: ... using a polar OPTIMA WAX column (30 m 3 0.25 mm 3 0.5 mm, Macherey-Nagel). The GC conditions are as followed ...
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bioRxiv - Biochemistry 2020Quote: ... 4°C for 45 min) and the supernatant was incubated for 25 min at 4°C with 2 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Neuroscience 2020Quote: ... The total proteins were precipitated and prepared for Polyacrylamide Gel Electrophoresis using protein precipitator and resuspension buffer PSB/TCEP (Protein solving buffer and (tris(2-carboxyethyl)phosphine) TCEP reducing agent) from the NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France). The elution fraction containing RNA was further used for PCR analysis (see below) ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µL of extracts were injected into a Nucleoshell RP 18 column (2.7 µm x 150 mm x 2 mm, Macherey-Nagel GmbH, Düren, Germany). A solvent system composed of A ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:100 RNase A (MACHEREY-NAGEL, 740505) was added in each sample for 15 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Germany) with a guard column (EC 4/3 NUCLEODUR C18 Gravity, 3 μm, Macherey-Nagel GmbH & Co. KG Germany), while the column temperature was kept at 40 °C in a thermostatic column compartment (Thermo Fisher UltiMate 3000) ...
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bioRxiv - Microbiology 2020Quote: ... The lysed culture was centrifuged at 46,000 x g for 1 hour and the supernatant was loaded onto a 1-ml Protino® Ni-NTA column (Macherey-Nagel). The protein was eluted with buffer A + 300 mM imidazole and protein-containing fractions were pooled and dialyzed overnight in buffer A with TEV protease (1/10 w/w ratio ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 100 μL of 1:1 diluted apical wash using the NucleoMag VET (Macherey-Nagel AG, Oensingen, Switzerland), according to the manufacturer’s guidelines ...
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bioRxiv - Zoology 2023Quote: ... Germany) or on a hydrophilic interaction liquid chromatography (HILIC) Nucleodur column (125 × 3 mm, 3 μm, Macherey-Nagel, Düren, Germany) for normalphase chromatography ...
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bioRxiv - Microbiology 2024Quote: ... we added 6 µL of rDNAse buffer and 0.6 µL rDNAse (Macherey-Nagel) per sample and incubated at 37°C for 10 min ...
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The complete mitochondrial genome of Spurilla braziliana MacFarland 1909 (Nudibranchia, Aeolidiidae)bioRxiv - Genomics 2023Quote: ... Japan (Figure 1) and preserved in NucleoProtect RNA (MACHEREY-NAGEL) for 5.5 months at -80 °C before DNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted using the pathogen Nucleic Acid Extraction kit (Macherey-Nagel, Düren, Germany) and eluted in 50 μL elution buffer ...
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bioRxiv - Microbiology 2019Quote: ... day 6 and day 9 postinoculation using pH indicator strips (Macherey-Nagel SARL, Hœrdt, France). We placed the pH indicator strip directly on the SAB agar plate for two minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was loaded onto 1 ml Ni-NTA agarose beads (Macherey-Nagel), pre-equilibrated with 10 ml wash buffer and rotated end-over-end for 1h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 mM NaCl and 1 mg/ml proteinase K (Macherey-Nagel, 740506) and 0.05 mg/ml RNase A (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA was extracted from 6 pools of 50 worms using an RNeasy® kit (Macherey-Nagel). Total RNA was used for oligo(dT ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Genetics 2019Quote: ... The frozen pellet was resuspended in 1 ml of RA1 buffer (Macherey-Nagel) supplemented 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Genomics 2023Quote: ... The ground tissues were lysed with 1 ml CF lysis buffer (MACHEREY-NAGEL) supplemented with 20 μl RNase and incubated for 1 h at 65°C under agitation ...
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bioRxiv - Genetics 2021Quote: ... Nucleic acid extraction was performed with the NucleoSpin DNA Insect Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Solid-phase extraction cartridges (Chromabond HR-X, 30mg, 1 ml, Macherey-Nagel, Dueren, Germany) were conditioned with 1 ml MeOH followed by 1 ml distilled HPLC-water ...
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bioRxiv - Plant Biology 2020Quote: ... The pellet was resuspended on ice in 1 ml NucleoZol (Macherey-Nagel, Düren, Germany) and incubated for 10 minutes at 65°C with shaking at 250 rpm ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from 1 × 106 cells using NucleoSpin DNA RapidLyse (MACHEREY-NAGEL). qPCR was performed with primers targeting the ND2 locus in the mitochondrial genome (5′-TGTTGGTTATACCCTTCCCGTACTA-3′ and 5′- CCTGCAAAGATGGTAGAGTAGATGA-3′ ...
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bioRxiv - Microbiology 2023Quote: ... 29-18-1 using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from ethylenediaminetetraacetic acid (EDTA)-anticoagulated blood using NucleoSpin Blood Quick Pure kit (Macherey-Nagel, Duren, Germany) and was stored in the gene bank run by Azabu University (Kanagawa ...
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bioRxiv - Microbiology 2023Quote: The nucleic acids were extracted from the FFPE tissue sections using the Nucleospin total RNA FFPE XS (Macherey-Nagel). RNA sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... The sample was then acidified using trifluoroacetic acid and peptides were purified using Chromabond C18 Microspin columns (Macherey-Nagel).
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bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from 1 mL overnight culture using NucleoSpin Plasmid Easypure columns (Macherey-Nagel) from the Plasmid Easypure Kit (Nucleospin).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with preequilibrated Protino Ni-NTA beads (1 ml resin, Macherey-Nagel) at 4°C for 1h ...
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bioRxiv - Microbiology 2023Quote: ... PA14 and PAK from ∼1 × 108 CFU using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel), as per manufacturer’s instructions ...
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Convergent evolution of distinct immune sensor systems for fungal polygalacturonases in BrassicaceaebioRxiv - Plant Biology 2020Quote: ... infiltrated leaves were harvested after 6 h for total RNA isolation with NucleoSpin® RNA Plus Kit (Macherey-Nagel). First strand cDNA was synthesized with RevertAid™ M-MuLV reverse transcriptase (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: RNA was isolated from 6-days-old seedlings using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Japan) equipped with an analytical CN-RP column (NUCLEODUR 100-5, CN-RP, 150 × 4.6, 5 µm column; Macherey-Nagel GmbH & Co. KG, Düren, Germany) and a UV-diode-array detector ...
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bioRxiv - Synthetic Biology 2022Quote: ... SLS was precipitated with trifluoroacetic acid (TFA, 1.5% final concentration) and peptides were purified using Chromabond C18 microspin columns (Macherey-Nagel). Acidified peptides were loaded on spin columns equilibrated with 400 μL acetonitrile and then 400 μL 0.15% TFA ...
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bioRxiv - Cell Biology 2019Quote: ... 1 ml supernatant containing Upa2 variant was added directly to the washed Ni-NTA (Macherey-Nagel) bead ...
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bioRxiv - Evolutionary Biology 2022Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Gibson assembly was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... Pre_S or FECD corneal endothelial tissues (Supplemental Table 1) by NucleoSpin RNA XS kit (Macherey-Nagel). cDNAs were prepared by reverse transcription ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM PMSF) by sonication followed by loading on to Ni-IDA column (Macherey-Nagel, Germany). Endonuclease G was eluted in gradient imidazole concentration (100-500 mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was first extracted from 1 x 105 cells using NucleoSpin Tissue (MACHEREY-NAGEL #740952) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... single colonies were picked and transferred into bacterial starter cultures and incubated for ∼6 h for mini-prep (Macherey-Nagel, NucleoSpin Plasimd Mini kit for plasmid DNA ...
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bioRxiv - Bioengineering 2022Quote: ... containing an insert (15 mm tip, wide opening, 0.2 mL, 6×31 mm, clear, Macherey-Nagel GmbH & Co. KG Germany) for High-Performance Liquid Chromatography (HPLC ...
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bioRxiv - Immunology 2023Quote: Cells from a confluent 6-well were lysed and RNA was purified using the NucleoSpin RNA Plus kit for RNA purification (740984.50, Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from the spleen of 4-6 weeks-old asymptomatic mice using nucleospin RNA II (Macherey-Nagel). The quality of RNA was checked with Bioanalyzer Agilent 2100 and RNAs with a RIN (RNA integrity number ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from 6-days-old seedlings grown in sterile culture using the RNA NucleoSpin Plant kit (Macherey-Nagel) according to the manufacturer’s instructions with on-column DNase treatment ...
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bioRxiv - Plant Biology 2021Quote: Ribonucleic acid (RNA) samples were isolated from seeds and leaves using the NucleoSpin® RNA Plant kit (Macherey-Nagel, Düren, Germany) according to manufacturer’s instructions ...