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Citations for Macherey-Nagel GmbH :
151 - 200 of 241 citations for 6 METHYL 4 4 NITRO PHENYL 2 OXO 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... equipped with a flame ionization detector (FID) and an Optima-s-3 GC capillary column (30 m x 0.25 mm x 0.25 µm - Macherey-Nagel GmbH & Co, Germany). Nitrogen (200 kPa ...
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bioRxiv - Evolutionary Biology 2020Quote: Fifteen microliters of extract were injected into an Agilent 1100 series HPLC and compounds were separated on an EC 150/4.6 NUCLEODUR® C18 Gravity column (3 μm, 150 mm x 4.6 mm, Macherey-Nagel, Düren, Germany). Cardenolides and bufadienolides were eluted at a constant flow of 0.7 ml/min at 30°C with an acetonitrile–H2O gradient as follows ...
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bioRxiv - Genetics 2021Quote: Genomic DNA from 5 males was extracted using the NucleoSpin Tissue XS kit (Macherey-Nagel, ref #740 901). To amplify the Rsp satDNA region ...
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bioRxiv - Plant Biology 2021Quote: ... dissolved and fractionated by RP-HPLC using a Nucleodur 100-5 C18ec 125×4.6 mm column (Macherey-Nagel). The ABA-containing fraction was collected ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM PMSF and purified by immobilized metal affinity chromatography using a 5 ml nickel column (Macherey-Nagel). The protein was eluted from the nickel column with solubilization buffer supplemented with 500 mM imidazole ...
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bioRxiv - Synthetic Biology 2023Quote: ... preparative HPLC was employed using a reversed-phase C18 column (Macherey-Nagel VP 250/21 Nucleodur 100-5) with a mobile phase solvent A (Water + 0.05% vol/vol trifluoroacetic acid (TFA)) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 ml of mutant culture from propagation round 3 were used for gDNA isolation using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). Whole genome sequencing was performed via the INVIEW Resequencing service for bacterial genomes up to 10 Mb with an Illumina standard genomic library provided by Eurofins Genomics (Ebersberg ...
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bioRxiv - Genetics 2024Quote: Total RNA from twenty heads of 3 day-old Drosophila adults for each genetic combination was purified using the RNA isolation kit of Macherey-Nagel (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions.1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... For Clr purification the initial purification was conducted using a 5 mL Protino™ Ni-NTA Column (Macherey-Nagel). Size-exclusion chromatography over a 16/600 Superdex 200 column (GE ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of plants 5 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Microbiology 2023Quote: ... slight adaptations were made from manufacturer’s instructions: half MCE filters were placed into individual 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel) and added with 1,200 µL MWA1 lysis buffer ...
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bioRxiv - Genetics 2019Quote: Total RNA from pools of 5 oocytes were extracted using the Nucleospin RNA XS kit according to the manufacturer’s protocol (Macherey-Nagel) and including a DNase1 treatment ...
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bioRxiv - Microbiology 2019Quote: ... compounds were separated by reverse-phase chromatography using Ultra Performance Liquid chromatography (UPLC IClass, Waters) using a Nucleodur C18ec column (2.0 mm × 150 mm, 5 μm particle size, Macherey-Nagel). Elution was achieved by increasing the acetonitrile/water (milliQ filtrated on 0.22 μm ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA was generated by isolating RNA from 5×106 HEK293T cells with the Nucleospin RNA isolation Mini kit (Macherey-Nagel)according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The drug concentration in samples was measured with high-performance liquid chromatography (HPLC) using a reverse phase C18 Nucleosil 100-5 (4.0 × 250 mm; 5.0 μm particle size, Macherey-Nagel, Germany) column and mobile phase consisting of MeOH/water (90/10 v/v ...
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bioRxiv - Biophysics 2022Quote: ... and the 3’-terminal poly A) were amplified by PCR and purified using the NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2019Quote: ... Cells were collected 5 days after transfection and the cell’s genomic DNA was prepared using NucleoSpin Tissue (MACHEREY-NAGEL & Co. KG).
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bioRxiv - Developmental Biology 2021Quote: ... and the reverse primer 5’AACCCAGGTAATGAACACAGTTTCTAT. PCR products were run on a gel (supplemental Fig. 2B) and bands were gel purified (Macherey-Nagel), inserted in a pCR-bluntII-Topo vector (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were extracted from positive colonies using 5 mL liquid cultures with the NucleoSpin Plasmid Purification kit (Macherey-Nagel; Ref.: 11932392) and sequenced (GATC ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of 5 days old seedlings (both controls and treated with NaCl) using the NucleoSpin RNA Plus (Macherey-Nagel). Reverse-transcription was performed using using the SuperScript III First-Strand VILO cDNA Synthesis Kit (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatants were transferred to fresh tubes and filtrated first through a 5 µm syringe PES filter (CHROMAFIL® Xtra PES, 5.0 µm, Macherey-Nagel), then through a 1 µm (Whatman™ Puradisc ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was filtered using a 0.45 µm syringe filter and loaded onto a 5 ml Protino Ni-NTA column (Macherey-Nagel, Düren, Germany). The column was washed until UV280 had reached a steady value and eluted with 500 mM imidazole ...
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bioRxiv - Molecular Biology 2019Quote: ... Starting from overnight grown liquid cultures of respective pAGM9121 constructs (prepared as described in section 5) plasmid mixtures were prepared using a NucleoSpin Plasmid Kit (Macherey-Nagel, Düren, DE). Following the preparation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... equipped with a photodiode array detector and a semi-preparative RP-Nucleodur C18 Htec column (5 µm, 250 × 10 mm, Macherey-Nagel, Düren, Germany) using a water-acetonitrile gradient ...
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bioRxiv - Cell Biology 2023Quote: ... equipped with C18 trap column packed in-house (1.5 cm, 360-μm outer diameter, 150-μm inner diameter, Nucleosil 100-5 C18; MACHEREY-NAGEL, GmbH & Co. KG) and an analytical C18 capillary self-made column (30 cm ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from ACE-grown cells (DSMZ medium 461 with 5 mM ACE) with the NucleoSpin® Microbial DNA kit (Macherey-Nagel, Düren, Germany) according to the manual and using wide-bore tips ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:100 RNase A (MACHEREY-NAGEL, 740505) was added in each sample for 15 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The lysed culture was centrifuged at 46,000 x g for 1 hour and the supernatant was loaded onto a 1-ml Protino® Ni-NTA column (Macherey-Nagel). The protein was eluted with buffer A + 300 mM imidazole and protein-containing fractions were pooled and dialyzed overnight in buffer A with TEV protease (1/10 w/w ratio ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 100 μL of 1:1 diluted apical wash using the NucleoMag VET (Macherey-Nagel AG, Oensingen, Switzerland), according to the manufacturer’s guidelines ...
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The complete mitochondrial genome of Spurilla braziliana MacFarland 1909 (Nudibranchia, Aeolidiidae)bioRxiv - Genomics 2023Quote: ... Japan (Figure 1) and preserved in NucleoProtect RNA (MACHEREY-NAGEL) for 5.5 months at -80 °C before DNA extraction ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was loaded onto 1 ml Ni-NTA agarose beads (Macherey-Nagel), pre-equilibrated with 10 ml wash buffer and rotated end-over-end for 1h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 mM NaCl and 1 mg/ml proteinase K (Macherey-Nagel, 740506) and 0.05 mg/ml RNase A (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... The frozen pellet was resuspended in 1 ml of RA1 buffer (Macherey-Nagel) supplemented 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Genomics 2023Quote: ... The ground tissues were lysed with 1 ml CF lysis buffer (MACHEREY-NAGEL) supplemented with 20 μl RNase and incubated for 1 h at 65°C under agitation ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Solid-phase extraction cartridges (Chromabond HR-X, 30mg, 1 ml, Macherey-Nagel, Dueren, Germany) were conditioned with 1 ml MeOH followed by 1 ml distilled HPLC-water ...
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bioRxiv - Plant Biology 2020Quote: ... The pellet was resuspended on ice in 1 ml NucleoZol (Macherey-Nagel, Düren, Germany) and incubated for 10 minutes at 65°C with shaking at 250 rpm ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from 1 × 106 cells using NucleoSpin DNA RapidLyse (MACHEREY-NAGEL). qPCR was performed with primers targeting the ND2 locus in the mitochondrial genome (5′-TGTTGGTTATACCCTTCCCGTACTA-3′ and 5′- CCTGCAAAGATGGTAGAGTAGATGA-3′ ...
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bioRxiv - Microbiology 2023Quote: ... 29-18-1 using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from 1 mL overnight culture using NucleoSpin Plasmid Easypure columns (Macherey-Nagel) from the Plasmid Easypure Kit (Nucleospin).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with preequilibrated Protino Ni-NTA beads (1 ml resin, Macherey-Nagel) at 4°C for 1h ...
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bioRxiv - Microbiology 2023Quote: ... PA14 and PAK from ∼1 × 108 CFU using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel), as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 1 ml supernatant containing Upa2 variant was added directly to the washed Ni-NTA (Macherey-Nagel) bead ...
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bioRxiv - Evolutionary Biology 2022Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Gibson assembly was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... Pre_S or FECD corneal endothelial tissues (Supplemental Table 1) by NucleoSpin RNA XS kit (Macherey-Nagel). cDNAs were prepared by reverse transcription ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM PMSF) by sonication followed by loading on to Ni-IDA column (Macherey-Nagel, Germany). Endonuclease G was eluted in gradient imidazole concentration (100-500 mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was first extracted from 1 x 105 cells using NucleoSpin Tissue (MACHEREY-NAGEL #740952) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was then loaded on a Protino® GST/4B column 1 ml (Macherey-Nagel) for pull-down experiments ...
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bioRxiv - Systems Biology 2019Quote: ... RNA was recovered from 1 x 106 cells using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The RNA was sent to Eurofins for RNA-seq analysis ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µl from each sample was spotted onto a POLYGRAM CEL 300 PEI-TLC plate (Macherey-Nagel), and developed with 0.5 M LiCl (Sigma) ...