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Citations for Macherey-Nagel GmbH :
1 - 50 of 97 citations for 6 Chloro 3 imino 2 3 dihydropyridazine 2 acetic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... from 2 to 3-day-old WT and mea-1-/-;dme-2-/- seedlings using NucleoSpin® Plant II (MACHEREY-NAGEL). 200 ng of DNA was subjected to fragmentation using ultrasonicator (Covaris ...
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bioRxiv - Biochemistry 2022Quote: ... Amino acids were separated on a reverse-phase C18 column (EC 250/2 NUCLEOSIL 100-3 C18HD, L=250 mm, ID: 2 mm; Macherey-Nagel). Samples (5 µL ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μL sample volume was injected into a Nucleodur HILIC column (250 mm * 2 mm, 3 μm particle size, Macherey-Nagel). Metabolites were eluted at 20 °C with an acetonitrile gradient (90% (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... using a polar OPTIMA WAX column (30 m 3 0.25 mm 3 0.5 mm, Macherey-Nagel). The GC conditions are as followed ...
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bioRxiv - Genomics 2021Quote: ... Menaquinones were then removed from the plate and analyzed using HPLC fitted with a reverse phase column (Macherey-Nagel, 2 mm x 125 mm, 3 µm, RP18) with methanol ...
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bioRxiv - Bioengineering 2022Quote: ... Germany) with a guard column (EC 4/3 NUCLEODUR C18 Gravity, 3 μm, Macherey-Nagel GmbH & Co. KG Germany), while the column temperature was kept at 40 °C in a thermostatic column compartment (Thermo Fisher UltiMate 3000) ...
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bioRxiv - Zoology 2023Quote: ... Germany) or on a hydrophilic interaction liquid chromatography (HILIC) Nucleodur column (125 × 3 mm, 3 μm, Macherey-Nagel, Düren, Germany) for normalphase chromatography ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting bottom organic phase was dried and on-line derivatized to fatty acid methyl ester by a chloroform/methanol (1:2)-TMSH solution (Macherey-Nagel) and analyzed by gas chromatography-mass spectrometry (Agilent 5977A-7890B) ...
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bioRxiv - Plant Biology 2021Quote: ... an EC 100/2 Nucleoshell Phenyl-Hexyl column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Plant Biology 2020Quote: ... LMW carbonyls were separated using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an Ekspert ultraLC 100 UHPLC system (AB SCIEX ...
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bioRxiv - Plant Biology 2023Quote: ... Separation was carried out using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an ekspert ultraLC 100 UHPLC system coupled to a QTRAP 4500 mass spectrometer (AB SCIEX ...
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bioRxiv - Genetics 2021Quote: ... PCR of genomic DNA extracted 3 days after nucleofection using NucleoSpin Tissue kit (Macherey-Nagel) was performed using specific primers and Sanger sequenced (Stabvida ...
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bioRxiv - Biochemistry 2024Quote: ... SSC was separated on a 250 x 3 mm 5 µm Nucleodur HTec C18 (Macherey-Nagel) column ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from ovaries of 3-day-old females using the NucleoSpin RNA isolation kit (Macherey-Nagel), following the instructions of the manufacturer ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleosil 100-5 N(CH3)2 column (Macherey-Nagel GmbH & Co, Düren, Germany) and eluted isocratically with 100% methanol containing 0.01% acetic acid at a flow rate of 1 ml min-1 ...
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bioRxiv - Genetics 2019Quote: Total RNA was extracted from ovaries of 3-day-old females using the NucleoSpin® RNA isolation kit (Macherey-Nagel), following the instructions of the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... as well as cKD TgISU1 (incubated with or without ATc at 1.5 μg/mL for 3 days) were extracted using Nucleospin RNA II Kit (Macherey-Nagel). The cDNAs were synthesized with 450 ng of total RNA per RT-PCR reaction using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Harvested bacteria were lysed using a French press (3 runs at 20.000 psi) in 1× LEW buffer (Macherey-Nagel, Düren, Germany) containing 5% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNA was extracted from mESCs and MEFs (3 biological replicates of WT and Atxn7l3−/− for each cell types) using the NucleoSpin RNA isolation kit (Macherey-Nagel), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ACE and its metabolites ANSA and acetoacetate were quantified by HPLC with an UV/VIS detector employing a Nucleosil 100-5 C18 HD column (240/3 mm, Macherey-Nagel) and an eluent at 0.5 ml min−1 containing 100 mM NaH2PO4 and 10 mM tetrabutylammoniumhydrogensulfate ...
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bioRxiv - Genomics 2023Quote: The genomic DNA was extracted from at least one million cells (typically 3 million, coverage 300x) using the NucleoSpin Tissue kit (Macherey-Nagel). To enrich for GBCs ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm×3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel), at 40°C ...
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bioRxiv - Microbiology 2023Quote: ... and 300 mg (JFC) of both fresh and treated frass (n = 3) using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) and following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm × 3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel), at 40 °C ...
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bioRxiv - Biochemistry 2021Quote: ... equipped with a flame ionization detector (FID) and an Optima-s-3 GC capillary column (30 m x 0.25 mm x 0.25 µm - Macherey-Nagel GmbH & Co, Germany). Nitrogen (200 kPa ...
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bioRxiv - Evolutionary Biology 2020Quote: Fifteen microliters of extract were injected into an Agilent 1100 series HPLC and compounds were separated on an EC 150/4.6 NUCLEODUR® C18 Gravity column (3 μm, 150 mm x 4.6 mm, Macherey-Nagel, Düren, Germany). Cardenolides and bufadienolides were eluted at a constant flow of 0.7 ml/min at 30°C with an acetonitrile–H2O gradient as follows ...
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bioRxiv - Plant Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm×3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel, Germany), at 40°C ...
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bioRxiv - Plant Biology 2021Quote: ... a Nucleoshell RP 18 plus reversed-phase column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Genomics 2020Quote: Total mRNA of edited HUDEP-2 cells was isolated using the NucleoSpin RNA kit (Macherey-Nagel) and reverse transcribed into cDNA ...
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bioRxiv - Developmental Biology 2022Quote: ... The supernatant was collected and loaded onto 2 mL pre-equilibrated Ni-NTA agarose (Macherey-Nagel). The mixture was rotated at 4 □ for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... elegans animals were transferred to 2 ml MN Bead Tubes Type A (Macherey-Nagel, Düren, Germany) and lysed using a Precellys Bead Beating system with an additional Cryolys cooling module (Bertin Instruments ...
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bioRxiv - Cancer Biology 2023Quote: ... BM DOHH2 were resuspended in RA1 buffer and tris(2-carboxyethyl)phosphine (TCEP) from Macherey-Nagel and stored at -80°C for less than 3 months ...
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bioRxiv - Neuroscience 2021Quote: ... 3 mice at each time point (ZT 1, 5, 9, 13, 17, 21) were sacrificed and RNA was extracted (Macherey-Nagel, Oensingen, Switzerland) according to manufacturer’s instruction ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 ml of mutant culture from propagation round 3 were used for gDNA isolation using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). Whole genome sequencing was performed via the INVIEW Resequencing service for bacterial genomes up to 10 Mb with an Illumina standard genomic library provided by Eurofins Genomics (Ebersberg ...
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bioRxiv - Genetics 2024Quote: Total RNA from twenty heads of 3 day-old Drosophila adults for each genetic combination was purified using the RNA isolation kit of Macherey-Nagel (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions.1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: The yeast expression vector pAG416-GAL-SARS-CoV-2-3CL was freshly prepared by Midiprep (Macherey-Nagel), and variants were introduced by a modified single primer site-directed mutagenesis method (Shenoy and Visweswariah ...
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bioRxiv - Microbiology 2023Quote: Separation was performed on an EC 100/2 Nucleoshell Phenyl-Hexyl column (2C×C100Cmm, 2.7Cμm; Macherey-Nagel) at 40°C ...
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bioRxiv - Biochemistry 2023Quote: ... SUV420H1 proteins were isolated by incubating the soluble fraction for 2 hrs with GST beads (Macherey-Nagel). The beads were washed with two column volumes of wash buffer (20 mM Tris ...
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bioRxiv - Genomics 2023Quote: ... DNA purification was carried out by adding a 2 ml mixture of wash buffer PW2 (MACHEREY-NAGEL), wash buffer B5 (MACHEREY-NAGEL) ...
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bioRxiv - Biophysics 2022Quote: ... and the 3’-terminal poly A) were amplified by PCR and purified using the NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from isolated islets from 2 mice per genotype with Nucleospin RNA plus XS (Macherey-Nagel). Complementary DNA was generated and linearly amplified from 3 ng total RNA using the Ovation RNA-seq V2 system (NuGEN technologies Inc. ...
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bioRxiv - Genetics 2020Quote: ... Samples were separated on a C18 column (EC 100/2 Nucleoshell RP-18, 2.7 μm, from Macherey-Nagel) using a gradient of 0.1% formic acid / isopropanol over 0.1% formic acid / water ...
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bioRxiv - Molecular Biology 2023Quote: ... the soluble fraction was incubated for 2 hours in batch with Ni2+-nitrilotriacetate (NTA) agarose resin (Macherey-Nagel). After extensive washing of the beads with lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected by gel electrophoresis on 2% Agarose/TAE and gel extracted by column purification (Macherey-Nagel). Amplicons were paired-end sequenced with 500 cycles on a MiSeq system (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected for 400-550 bp using a 2% agarose/TAE gel and column purification (Macherey-Nagel). Libraries were sequenced as above ...
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bioRxiv - Genomics 2022Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...