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Citations for Macherey-Nagel GmbH :
301 - 350 of 492 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... mCherry+ cells were sorted 48 hr later and their total RNA isolated using NucleoZOL (Macherey-Nagel). The mRNA fraction was then enriched using a NEBNext Poly(A ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from the cells with NucleoSpin RNA Mini Kit (Macherey-Nagel; MN 740955.250), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Total DNA was extracted from cells using the commercially available NucleoSpin Triprep kit (Macherey-Nagel, Germany). To quantify levels of mtDNA ...
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bioRxiv - Developmental Biology 2023Quote: Total RNAs from cultured human iPS cells were extracted using NucleoSpin RNA Plus XS (Macherey-Nagel). The 100 ng of total RNAs were used as starting materials to generate RNA-seq libraries with the TruSeq Stranded mRNA LT sample prep kit (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed mechanically via bead-beating and DNA was purified on columns (Macherey-Nagel, Germany). A robotized platform (Biomek4000 ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from 1 mL overnight culture using NucleoSpin Plasmid Easypure columns (Macherey-Nagel) from the Plasmid Easypure Kit (Nucleospin).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with preequilibrated Protino Ni-NTA beads (1 ml resin, Macherey-Nagel) at 4°C for 1h ...
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bioRxiv - Microbiology 2023Quote: ... PA14 and PAK from ∼1 × 108 CFU using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel), as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... total RNA of hCMEC/D3 cells were extracted using NucleoSpin RNA kit (Macherey-Nagel; catalog no. 740955.50) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA from cell pellets was extracted and purified using the Nucleospin RNA protocol from Macherey-Nagel kit (740955) ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from U2OS and HeLa cell pellets using a Nucleospin RNA isolation kit (Macherey-Nagel) according to the manufacturer’s instructions and the RNA concentration was measured using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: RNA of naïve and polyclonally activated CD8+ T cells was purified using NucleoSpin RNA kit (Macherey-Nagel) according to manufacturer’s protocol and submitted to the Scientific Genomics Platform (Max Delbrück Center for Molecular Medicine ...
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bioRxiv - Cancer Biology 2020Quote: ... ovarian carcinoma cells had their RNA chemically purified using the Nucleospin RNA Plus kit (Macherey-Nagel: 740984.50) as recommended by the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... and viral RNA from cell lysate was extracted using the Nucleospin RNA plus mini kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... RNA from sorted MAIT cells was isolated using the Nucleospin RNA isolation kit (Macherey-Nagel, Düren, Germany) as per the manufacturer’s instructions and stored at −80 °C ...
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bioRxiv - Molecular Biology 2019Quote: Cells were lysed for RNA 36h-40h post-transfection with 350µL RNA lysis buffer LBP (Macherey-Nagel), and RNA were extracted with the NucleoSpin RNA Plus kit (Macherey-Nagel ...
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bioRxiv - Physiology 2020Quote: ... Genomic DNA from 32 MPM cell lines was extracted with Nucleospin Blood kit (Macherey-Nagel, Düren, Germany) and 500 ng were hybridized to Affymetrix CytoScanHD Arrays (Affymetrix ...
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bioRxiv - Microbiology 2021Quote: ... The intracellular and extracellular RNA from cells was isolated using respective kits (MACHEREY-NAGEL GmbH & Co. KG) and the SARS-CoV-2 RNA was quantified using a commercial kit (LabGun™ COVID-19 RT-PCR Kit ...
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bioRxiv - Neuroscience 2019Quote: ... Cell lysis and RNA extraction were performed 24h post-activation using Nucleospin RNA extraction kit (Macherey-Nagel). Quality of RNA was confirmed on Agilent TapeStation (RINe>8).
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 5×106 B16F10 tumor cell lines using NucleoSpin® Tissue (MACHEREY-NAGEL), according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Total RNAs from harvested cells were extracted using the NucleoSpin RNA extraction kit (Cat. # 740955.250, MACHEREY-NAGEL), and 0.4-1 μg RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Cat ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: RNA was isolated from MEFs or NIH3T3 cells using the Nucleospin RNA plus kit (Macherey-Nagel, 740984.5). Subsequently ...
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bioRxiv - Immunology 2020Quote: Viral RNA was extracted from the infected cells using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel) and Covid-19 virus detection was performed using One-Step qRT-PCR SuperScript™ III Platinum™ (Life Technologies ...
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bioRxiv - Genetics 2021Quote: ... coli (High Efficiency) cells and subsequent plasmid isolation was performed using the NucleoSpin Plasmid kit (Macherey-Nagel).
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bioRxiv - Immunology 2022Quote: ... lymph nodes and bone marrow cells by manual extraction with the NucleoSpin® Tissue kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was extracted from liver using the Genomic DNA from organs and cells Kit (Macherey-Nagel) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: Cells were harvested and total RNA was isolated using either the Nucleospin RNA mini kit (MACHEREY-NAGEL) or the Quick-DNA/RNA™ Viral kit (Zymo Research ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from sorted Nkp46+ or CD8+ cells with the NucleoSpin RNA XS kit (Macherey-Nagel) and cDNA was prepared using the SMARTer Ultra Low RNA kit (Clontech Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was extracted from cells corresponding to 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel).
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bioRxiv - Immunology 2024Quote: ... gDNA from T cells and frozen liver biopsies was extracted with NucleoSpin® Blood kit (Macherey-Nagel) and TCRβ sequencing was performed as previously described45.
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bioRxiv - Cell Biology 2019Quote: ... 1 ml supernatant containing Upa2 variant was added directly to the washed Ni-NTA (Macherey-Nagel) bead ...
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bioRxiv - Evolutionary Biology 2022Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Gibson assembly was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... Pre_S or FECD corneal endothelial tissues (Supplemental Table 1) by NucleoSpin RNA XS kit (Macherey-Nagel). cDNAs were prepared by reverse transcription ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM PMSF) by sonication followed by loading on to Ni-IDA column (Macherey-Nagel, Germany). Endonuclease G was eluted in gradient imidazole concentration (100-500 mM ...
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bioRxiv - Cell Biology 2020Quote: ... Total RNA from cells of interest was isolated using the NucleoSpin RNA Kit from Macherey-Nagel (Düren, Germany) according to manufacturer’s recommendation ...
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bioRxiv - Immunology 2021Quote: ... RNA extraction (two replicates per cell line) was performed using the Nucleospin® RNA Plus Kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was collected from the DT40 cells using a NucleoSpin RNA kit (Macherey-Nagel GmbH & Co., Germany) and subjected to complementary DNA synthesis and quantitative PCR using a ReverTra Ace qPCR RT Kit (Toyobo Life Science ...
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bioRxiv - Neuroscience 2021Quote: mRNAs of O4+ MACSorted cells were extracted using the Macherey-Nagel NucleoSpin RNA XS kit (740902.50, Macherey-Nagel) and quantified with Nanodrop spectrophotometer ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from N/Tert or HFK cells using the NucleoSpin RNA extraction kit (Macherey-Nagel). RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted from GYP treated GLUTag cells using the NucleoSpin® RNA kit (Macherey-Nagel, UK) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Genomic DNA was extracted using the NucleoSpin tissue mini kit for DNA from cells and tissue (Macherey-Nagel). DNA integrity was assessed by TapeStation (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA (gDNA) was isolated from sorted and unsorted cells using the Nucleospin Tissue Mini Kit (Macherey-Nagel) and sgRNA sequences were retrieved by a nested PCR approach that pre-amplified sgRNAs in a first round with primers specific to the lentiGuide-Puro construct (5’-AGAGGGCCTATTTCCCATGA-3’ ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from infected and non-infected HCT8 cells using NucleoSpin RNA Kit (Macherey-Nagel, Germany). An on-column DNase digestion with a RNase-free DNase was included in the process described by the fabricant to remove any genomic DNA contamination in RNA samples ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from 2.5×105 HEK293T cells was purified using a Nucleo Spin Tissue XS kit (MACHEREY-NAGEL). Using 25 mg of human postmortem tissue ...
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bioRxiv - Immunology 2020Quote: Viral RNA was extracted from infected cells using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel, Hoerdt, France) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: To clone the NERCLIN cDNA we extracted total RNA from 143B cells using Mini spin kit (Macherey-Nagel). Then RNA was reverse transcribed using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher scientific) ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA was isolated using the NucleoSpin Tissue – Mini kit for DNA from cells and tissue (MACHEREY-NAGEL). Genomic DNA was sheared with a 30-G syringe ...
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bioRxiv - Microbiology 2022Quote: Total DNA was isolated from frozen cell pellets using the NucleoSpin Microbial DNA Kit (Macherey-Nagel, Düren, Germany). Methods for DNA quality control and quantification were reported previously [28] ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from the cells with a PGTX solution42 and purified using NucleoSpin RNA (MACHEREY-NAGEL). cDNA was synthesized from 1 μg of total RNA using ReverTra Ace qPCR RT Master Mix (TOYOBO) ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of homogenate was set aside as total cell (T) and mixed with NucleoZOL (Macherey-Nagel, #740404.200) before storage at -80°C ...