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Citations for Macherey-Nagel GmbH :
201 - 250 of 2323 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: RNAs from mock infected or infected (MOI=0.001) cell culture supernatant were extracted using the Nucleospin Dx Virus RNA purification kit (Macherey-Nagel). Then qRT-PCR was performed in triplicate as described20 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the cells were collected from the plate and plasmid pools were extracted using the NucleoBond Xtra Midi Kit (Macherey-Nagel) according to the manufacturer’s recommendation.
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bioRxiv - Neuroscience 2021Quote: Cell lysis and RNA extraction were performed on macrophage samples 24h post-activation using Nucleospin RNA extraction kit (Macherey-Nagel). Quality of RNA was confirmed on Agilent TapeStation (RINe>8) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from ΔbolA and control cell line was isolated independently six times using NucleoSpinTM RNA isolation kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: RNAs from mock infected or infected (MOI=0.01) A549-hACE2 cell culture supernatant were extracted using the Nucleospin Dx Virus RNA purification kit (Macherey-Nagel). Then qRT-PCR was performed in triplicate as described20 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with PBS and lysed with RNA extraction buffer (Nucleo spin RNA extraction kit, Macherey-Nagel, Cat# 740955). Total RNA was extracted according to the manufacturer instructions ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA was generated by isolating RNA from 5×106 HEK293T cells with the Nucleospin RNA isolation Mini kit (Macherey-Nagel)according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: We extracted RNA from live cells for bulk RNA-sequencing using the NucleoSpin totalRNA FFPE micro kit (Macherey-Nagel #740969.50) ...
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bioRxiv - Genetics 2023Quote: Total RNA was isolated from the cultured cells using the Nucleospin RNA Plus kit (cat. 740984, Macherey-Nagel, CA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Total RNA from BMDMs and RAW 264.7 cells was isolated using the NucleoSpin RNA II kit (Macherey-Nagel, Catalog #740955). The cDNA of pre-mRNA was synthesized by the use of random hexamer primers (Thermo-Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested in RIPA with 1X HALT or the RNA lysis buffer from the Nucleospin RNA kit (Macherey-Nagel).
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from 200 μL of pelleted red blood cells diluted 1:1 in PBS1X using the DNA Nucleospin blood extraction kit following the manufacturer’s instructions (Macherey-Nagel). The extracted DNA was then stored at -20°C for later use.
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bioRxiv - Neuroscience 2024Quote: ... RNA from tissues or cells was isolated using an RNA isolation kit (NucleoSpin RNA, Macherey-Nagel GmbH & co.KG, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Total RNAs were extracted from about 6 ×106 HepaRG cells using a Nucleospin RNA isolation Kit (Macherey-Nagel, Hoert, France) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: RNA from human prostate cancer cell lines was extracted using NucleoSpin® RNA isolation kit from Macherey-Nagel (Ref: 740955.240C), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated from wt and Uhrf1- or Usp7-knockdown BHK cells using the nucleospin triprep kit from Macherey-Nagel. 500 ng of total RNA was reverse transcribed with a high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Bioengineering 2021Quote: Cell pellets were washed and lysed using RA1 RNA extraction buffer and purified using the NucleoSpin RNA kit (MAcherey-Nagel; 740955.250) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... the amplified plasmid library was extracted from bacterial cells using 4 reactions of Midiprep kit (Macherey-Nagel NucleoBond Xtra Midi Plus).
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bioRxiv - Genomics 2020Quote: Cells were rinsed with phosphate buffer saline (PBS) before to be homogenized in RNA lysis buffer (NucleoSpin-RNA Kit, Macherey-Nagel), supplemented with 1% β-Mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were collected into the RA1 lysis buffer and extracted using the Nucleospin RNA Plus Extraction kit (740984, Macherey-Nagel).
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were propagated in Eschericia coli DH10B cells using standard procedures and purified using NucleoBond Xtra Midi EF Kit (Macherey-Nagel).
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bioRxiv - Genomics 2020Quote: ... the genomic DNA from the edited cells was isolated after the transduction for eight days using a DNA isolation kit (NucleoSpinBlood - Macherey-Nagel). The target region for base editing was amplified using the respective primers (Sup Tables 2 and 3) ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNAs were extracted from yeast cells disrupted by bead beating and purified using the Nucleospin RNA II kit (Macherey-Nagel). For RNA-seq analysis ...
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bioRxiv - Evolutionary Biology 2021Quote: ... isolation of vRNA from 150 µL cell-free supernatants was carried out with the NucleoSpin RNA virus kit (Macherey-Nagel, 740956) as described in the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... flanking Cas9 targeted site was amplified from genomic DNA extracted from wild type MCF10A cells (NucleoSpin tissue extraction kit, Macherey-Nagel), Puro-T2A were amplified from the custom-made plasmid MXS Puro bGHpA using primers containing T2A sequence ...
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bioRxiv - Cell Biology 2020Quote: Cells were rinsed with phosphate buffer saline (PBS) before to be homogenized in RNA lysis buffer (NucleoSpin RNA Kit, Macherey-Nagel), supplemented with 1% β-Mercaptoethanol ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from flow-sorted naive CD8+ T cells (n = 300 per condition) using a NucleoSpin RNA XS Kit (Macherey-Nagel), and cDNA was synthesized using Reverse Transcription Master Mix (Fluidigm) ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNA was extracted from mESCs and MEFs (3 biological replicates of WT and Atxn7l3−/− for each cell types) using the NucleoSpin RNA isolation kit (Macherey-Nagel), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from U251 cells (SETD6 control vs. KO or Empty vs. TWIST1 WT) using the NucleoSpin RNA Kit (Macherey-Nagel). Samples were prepared in triplicates (SETD6 KO ...
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bioRxiv - Immunology 2022Quote: ... The surviving cells were expanded for 24 h and DNA was extracted using a NucleoSpin Tissue kit (Macherey-Nagel, Cat#740952). An additional eight biological replicates were similarly prepared but did not undergo killing by TC5A87 ...
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bioRxiv - Immunology 2022Quote: ... Transfection-grade plasmid DNA was isolated from XL10Gold cell overnight culture with the NucleoBond™ Xtra Midi Kit (Macherey-Nagel, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... The ligated products were transformed into XL1 blue bacterial cells by electroporation followed by isolating the plasmids from single bacterial colonies using NucleoBond Xtra Midi kit (Macherey-Nagel). The Cas9-sgRNA plasmids were validated by BbsI restriction analysis and confirmed by Sanger sequencing ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from control and IMCD PCM1 KO cells before SAG treatment and 24 h after SAG treatment using NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: HIV RNA was extracted from plasma samples collected from either HIV-1 infected or uninfected humanized hNSG mice transplanted with CD4+ T cells from PWH in the absence or presence of immunotherapy using NucleospinTM RNA Virus kit (Cultek, Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated from confluent cells from one well of a 6-well plate with the NucleoSpin RNA isolation kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA from three replicates of PRMT7-KO2 and control PC3 cells was extracted using the NucleoSpin RNA kit (Macherey-Nagel). mRNA quality control checks and NGS for RNA-seq analysis were performed at the NIMgenetics facility (Madrid ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA was successfully isolated from blood cells of 34 14-day-old great tits with NucleoSpin RNA Plus Kit (Macherey-Nagel). Packed blood cells (10 µl per sample ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from Kasumi-1 cells after 2 days after shRUNX1::ETO knockdown was induced with doxycycline using the Nucleospin RNA kit (Macherey-Nagel). cDNA was synthesised using Superscript II (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The viruses were characterized by isolating viral RNA from 150-µL aliquots of the cell culture supernatant with the NucleoSpin RNA Virus kit (Macherey-Nagel) followed by conventional RT-PCR and bidirectional Sanger sequence analysis of the inserted SARS-CoV-2 sequences and flanking regions ...
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bioRxiv - Genomics 2023Quote: The genomic DNA was extracted from at least one million cells (typically 3 million, coverage 300x) using the NucleoSpin Tissue kit (Macherey-Nagel). To enrich for GBCs ...
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bioRxiv - Bioengineering 2023Quote: ... supernatant was removed and cell pellets were used for plasmid isolation by using NucleoBond□ Xtra Midi Plasmid DNA Purification Kit (Macherey-Nagel) according to the protocol supplied by the kit.
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from T-REx-293 and A549 cells using the NucleoSpin RNA Mini kit for RNA purification (Macherey-Nagel). 500 ng of total RNA was used for cDNA synthesis using Maxima™ Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: Total RNA was prepared from isolated colonic epithelial cells of Hsp60Δ/ΔIEC and Hsp60fl/fl mice on day 2 with the column-based NucleoSpin RNAII kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from cells at the indicated time points post stimulation and/or infection using the NucleoSpin RNA extraction kit (Macherey-Nagel) as directed by manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products for each single cell clone were combined and purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Afterward using a second round of PCR ...
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bioRxiv - Cell Biology 2024Quote: Total RNA from MCF7 cells was obtained with a Macherey-Nagel™ Total RNA Isolation kit (Macherey-Nagel, Germany, Cat # 740955.50) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... DNA expression plasmids were extracted and were purified from bacterial cells with NucleoBond® Extra Midi Plus kit (Macherey-Nagel, Germany) and they were stored at -20°C until their use for transient transfection into mammalian cells.
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA genes of AOB and AOA was amplified with primers amoA-1F/amoA-2R (66 ...
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bioRxiv - Microbiology 2020Quote: ... Clonal virus populations were characterized by isolating viral RNA from 150 µl aliquots of the cell culture supernatant with the NucleoSpin® RNA Virus kit (MACHEREY-NAGEL) followed by conventional RT-PCR and bidirectional Sanger sequence analysis.