Labshake search
Citations for Macherey-Nagel GmbH :
201 - 250 of 277 citations for Human LACC1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... BACs were extracted from bacterial culture using the NucleoBond® Xtra Midi plasmid extraction kit (Macherey-Nagel) according to the manufacturer’s provided instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... Protino® Ni-NTA Agarose and NucleoBond® Midi Plasmid DNA Purification Kit were from Macherey-Nagel, Germany ...
-
bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was extracted as pooled libraries using NucleoBond Xtra Maxi EF kit (Macherey-Nagel, Duere, Germany) and stored at −20 ℃.
-
bioRxiv - Microbiology 2023Quote: ... The pBAC infectious clone plasmid was extracted and purified using NucleoBond Xtra Maxi prep kit (Macherey-Nagel). The plasmid was then sequenced using Primordium Labs “Large” whole plasmid sequencing service ...
-
bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA was extracted from cells corresponding to 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel).
-
bioRxiv - Neuroscience 2023Quote: ... Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US).
-
bioRxiv - Neuroscience 2024Quote: Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US). Plasmids expressing red fluorescent protein mRuby341 at 25–100 ng/µL under CAG promoter in combination with ASAP328 voltage sensor at 30–100 ng/µL under either CAG ...
-
bioRxiv - Microbiology 2020Quote: DNA was extracted from six human fecal samples using the NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Germany), as described previously [36] ...
-
bioRxiv - Cell Biology 2020Quote: ... were purified using an endonuclease free plasmid extraction kit (NucleoBond Xtra Maxi Plus EF, Macherey-Nagel, cat# 740426.10).
-
bioRxiv - Neuroscience 2021Quote: ... High yield of plasmid DNA was obtained using the NucleoBond® Xtra Maxi Plus EF (Macherey-Nagel, 740426.50). 70μg AAV packaging plasmid ...
-
bioRxiv - Immunology 2022Quote: ... The plasmid DNA was extracted from the bacterial cultures using NucleoBond™ Xtra Midi Plus (Macherey-Nagel, 740412.10) according to the manufacturer’s guidelines ...
-
bioRxiv - Neuroscience 2021Quote: ... Endotoxin-free plasmid DNA was purified from clones with correct sequences (Nucleobond Xtra Maxi EF, Macherey-Nagel Inc.), and re-suspended in endotoxin-free water.
-
bioRxiv - Genetics 2021Quote: ... The final plasmid library DNA was isolated from the culture using the Nucleobond Xtra Maxi kit (Macherey-Nagel).
-
bioRxiv - Microbiology 2022Quote: Large-scale purification of BAC/YAC plasmid DNA was done using a NucleoBond® Xtra BAC (Macherey-Nagel) kit ...
-
bioRxiv - Pathology 2023Quote: ... we isolated mtDNA from postnuclear fractions with the alkaline denaturation-neutralization procedure (Nucleospin Plasmid Mini kit, Macherey-Nagel). Liver tissue was homogenized and nuclei pelleted (1000g 10 min ...
-
bioRxiv - Neuroscience 2024Quote: ... Maxi-preps of the plasmid construct were isolated using a Nucleobond Xtra Maxi Plus EF kit (Macherey-Nagel). The full coding frame of all pRK5-rPanx1 and pRK5-mPanx1 constructs were sequenced at the University of Calgary Sequencing Core using the primers CMV-F (5′-CGCAAATGGGCGGTAGGCGTG-3′ ...
-
bioRxiv - Molecular Biology 2024Quote: ... extracted by using the NucleoSpin® Plasmid or NucleoBond® Xtra Midi Kit (Macherey-Nagel; #740588.250 and #740410.50) and controlled by Sanger Sequencing (Eurofins Genomics or Seqlab Microsynth).
-
bioRxiv - Molecular Biology 2024Quote: ... The pUC57 mini plasmid used as carrier cDNA for transfections was gigaprepped as per kit instructions (Macherey-Nagel). The normalized ACE-tRNA library cDNA at 50 ng/μL was mixed at equal volumes with pUC57 mini carrier plasmid at 150 ng/μL to give final concentrations of 25 ng/μL and 75 ng/μL of the library and carrier plasmids respectively.
-
Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... were grown overnight at 37 °C and plasmid was subsequently purified using the NucleoBond Xtra Midi kit (Macherey-Nagel). Plasmid DNA was solubilized in 150 µl of TE buffer and quantified using NanoDrop ND-1000 spectrophotometer (Celbio) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Bacteria were collected and the plasmid DNA was purified using NucleoBond® Xtra Midi kit (MACHEREY-NAGEL, cat. 740410.50). The transformation efficiency was estimated by plating 1/10000 of the transformation reaction onto 15 cm LB-agar plate with 100 µg/ml ampicillin and counting number of clones after incubation overnight at 37°C.
-
bioRxiv - Neuroscience 2022Quote: ... The plasmid DNA was prepared with the High copy Midi-Prep (Nucleobond Xtra Midi Plus, Macherey-Nagel, Düren, Germany).
-
bioRxiv - Molecular Biology 2022Quote: ... All PCR fragments for cloning and plasmids were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and GenElute™ Plasmid Miniprep Kit (Merck) ...
-
bioRxiv - Cancer Biology 2022Quote: ... plasmid DNAs were recovered from ampicillin resistant colonies using a kit (NucleoBond Xtra Midi, Macherey-Nagel GmbH, Düren, Germany), and the nucleotide sequences of each construct confirmed as correct by Sanger direct cycle sequencing using a U6-specific sequencing primer.
-
bioRxiv - Molecular Biology 2023Quote: ... and linearized plasmid was purified from the gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). In-Fusion reaction was prepared as follows ...
-
bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA containing the ACE-tRNA TTT sequences was midiprepped with the NucleoBond PC 20 miniprep kit (Macherey-Nagel). 16HBE14o-cells were diluted to 6 x 105 cells per mL with 1 mL added per well of a coated 6-well plate as previously described22 ...
-
bioRxiv - Immunology 2021Quote: ... apical washes of Human coronavirus 229E (HCoV-229E) and MERS-CoV-infected hAECs were extracted using the NucleoMag VET (Macherey-Nagel) according to manufacturer’s guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from snap frozen mouse or human liver tissue using NucleoSpin RNA Mini kit (Macherey-Nagel, Düren, Germany). First strand cDNA was synthesized from 1 μg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... the cells were collected from the plate and plasmid pools were extracted using the NucleoBond Xtra Midi Kit (Macherey-Nagel) according to the manufacturer’s recommendation.
-
bioRxiv - Microbiology 2019Quote: ... difficile strains was done using standard methods.33 Routine DNA extractions were performed using the Nucleospin Plasmid Easypure (Macherey-Nagel) and DNeasy Blood and Tissue (Qiagen ...
-
bioRxiv - Cancer Biology 2019Quote: ... guide RNAs and Cas9 were expressed from plasmids that were purified with the NucleoBond® Xtra Midi Kit (Macherey-Nagel). In the remaining mixes ...
-
bioRxiv - Physiology 2023Quote: ... We prepared a large amount of these plasmid vectors using a NucleoBond Xtra Midi Plus kit (Macherey-Nagel, Düren, Germany). Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2024Quote: ... pcDNA5/FRT-SUGCT and pOG44 plasmid DNA was purified by NucleoBond Xtra Midi Plus EF DNA purification Kit (Macherey-Nagel). Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen ...
-
bioRxiv - Cell Biology 2024Quote: ... A large amount of these plasmid vectors was prepared using a NucleoBond Xtra Midi Plus kit (Macherey-Nagel, Düren, Germany). Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific).
-
bioRxiv - Biophysics 2024Quote: ... NucleoSpin® plasmid isolation kit and NucleoSpin® gel and PCR clean-up kit were purchased from Macherey-Nagel (Germany). PierceTM protease inhibitor tablets EDTA free and Pierce™ high capacity Ni-IMAC resin were purchased from Thermo Scientific (international) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2021Quote: ... the amplified plasmid library was extracted from bacterial cells using 4 reactions of Midiprep kit (Macherey-Nagel NucleoBond Xtra Midi Plus).
-
bioRxiv - Cell Biology 2022Quote: ... Plasmids were propagated in Eschericia coli DH10B cells using standard procedures and purified using NucleoBond Xtra Midi EF Kit (Macherey-Nagel).
-
bioRxiv - Genetics 2022Quote: ... cells were scraped from plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel). The resulting plasmid DNA was used for yeast transformation.
-
bioRxiv - Genetics 2022Quote: ... the cells were scraped from the plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel).
-
bioRxiv - Genetics 2019Quote: ... Endotoxin-free plasmid DNA from sequence-verified clones was isolated using the Nucleobond Xtra Midi EF Kit (Macherey-Nagel, Düren Germany), ammonium acetate precipitated and re-suspended in MaxCyte buffer (MaxCyte Inc. ...
-
bioRxiv - Genetics 2021Quote: ... 500ml of LB media with Ampicillin were inoculated with the rest of the mixture and incubated overnight for subsequent plasmid purification using the NucleoBond Xtra Maxi Plus kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Synchronized ring stage parasites were transfected with 100 – 200 μg plasmids purified by NucleoBond Xtra Midi EF midiprep kit (Macherey-Nagel) by electroporation using Bio-Rad laboratories gene pulser X cell31 ...
-
bioRxiv - Immunology 2020Quote: ... was expressed in 293T cells from a plasmid kindly provided by Florian Krammer and purified using Ni-NTA Agarose (Macherey-Nagel), as previously published (Stadlbauer et al. ...
-
bioRxiv - Immunology 2022Quote: ... Transfection-grade plasmid DNA was isolated from XL10Gold cell overnight culture with the NucleoBond™ Xtra Midi Kit (Macherey-Nagel, Germany).
-
bioRxiv - Genetics 2022Quote: ... 500 ml of LB media with Ampicillin were inoculated with the rest of the mixture and incubated overnight for subsequent plasmid purification using the NucleoBond Xtra Maxi Plus kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel). The selection markers for fungal transformation were cloned into digested plasmids by using the Rapid DNA Ligation Kit (Thermo) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The ligated products were transformed into XL1 blue bacterial cells by electroporation followed by isolating the plasmids from single bacterial colonies using NucleoBond Xtra Midi kit (Macherey-Nagel). The Cas9-sgRNA plasmids were validated by BbsI restriction analysis and confirmed by Sanger sequencing ...
-
bioRxiv - Neuroscience 2023Quote: ... The positive clones were grown in 300 mL LB cultures and the plasmids were extracted using the NucleoBond Xtra Midi EF kit (Macherey-Nagel). The plasmids were sent out for sequencing again before being packaged into AAVs.