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Citations for Macherey-Nagel GmbH :
2251 - 2298 of 2298 citations for Estradiol Serum ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from tobacco leaf disks (sampled from glasshouse grown plants and quickly frozen in liquid nitrogen) using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Fisher Scientific, UK). cDNA was synthesized using 1 μg total RNA in 20 μl using the oligo-dT primer according to the protocol in the RevertAid Reverse Transcriptase kit (Fermentas ...
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bioRxiv - Developmental Biology 2022Quote: ... All plasmids were cloned into the DH5a strain (Enzynomics) and purified with a NucleoBond Xtra Midi Plus kit (MACHEREY-NAGEL GmbH & Co. KG, Dueren, Germany) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: RNA from VTMs or swab-immersed TE buffer or cell culture supernatant was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG; 740956.250). The SARS-CoV2 RNA was quantified using (LabGun™ COVID-19 RT-PCR Kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The leaf material was lysed with sterile sand and 7mm glass beads before DNA was extracted with the NucleoMag 96 plant kit (Macherey-Nagel Gmbh & Co., Düren, Germany) using the KingFisher Flex magnetic particle processor (Thermo Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... and the 3’-terminal poly A) were amplified by PCR and purified using the NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Then we co-extracted the total RNA and DNA of the biological replicates on up to 0.35 g frozen soil sample with the NucleoBond RNA Soil Mini kit (Macherey-Nagel GmbH & Co. KG Dueren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from 12-day-old plantlets grown in vitro using the RNA Plus Kit (Macherey-Nagel GmbH & Co. KG, Düren, NRW, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplified regions of CRISPR-C unedited samples and the specific bands derived from the amplification of the deletion scar and circular junction sites were dissected and DNA products purified by NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL Düren, Nordrhein-Westfalen, Germany) and sent for Sanger sequencing at Eurofins Genomics ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid preparation and DNA gel extraction were performed using the Nucleospin Plasmid and the Nucleospin Extract II kits (Macherey-Nagel, Lab Supplies Scientific SA, Hellas). Restriction enzymes were from Takara Bio (Lab Supplies Scientific SA ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were separated on 2.5% agarose gels and extracted using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany, cat. no. 740609). The purified PCR products were then cloned using a Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean‑up (Macherey-Nagel, Düren, Germany). Following digestion with restriction enzymes ...
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bioRxiv - Evolutionary Biology 2019Quote: The pH of individual reaction mixtures was determined via TRITEST L pH 1–11 pH papers (Macherey-Nagel) directly after the reaction ...
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bioRxiv - Biochemistry 2022Quote: ... Table 1) followed by column purification using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). After sequencing the purified PCR product ...
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bioRxiv - Microbiology 2019Quote: For the hybrid LAMP assay total DNA was extracted from two or three frozen cedar baits using the Machery-Nagel Plant Kit II (Macherey-Nagel GmbH & Co. KG, Düren, Germany) and the manufacturer’s recommended protocol for plant material ...
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bioRxiv - Microbiology 2022Quote: Swab tips were excised using sterile scissors and were subject to DNA extraction using the NucleoSpin Soil DNA extraction kit (Macherey-Nagel Inc., Allentown, PA; catalogue: 740780) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). In vitro methylation experiments were performed for 10 min (DNA ...
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bioRxiv - Biochemistry 2023Quote: ... containing 1819 adenine bases) was amplified by PCR and purified using a NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) with Milli-Q water ...
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bioRxiv - Biochemistry 2023Quote: ... and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). In vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer (New England Biolabs Inc. ...
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bioRxiv - Plant Biology 2023Quote: ... RNA were extracted at different germination timepoints and in different tissues using the NucleoSpin® RNA Plant and Fungi Kit (Macherey Nagel, DuiJren, Germany) as described above but including a DNAse treatment (Macherey-Nagel, rDNAse set, DuiJren, Germany). RNA were quantified using a using a NanoDrop ND-1000 (NanoDrop Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Total nucleic acids were extracted from mouse samples submitted to IDEXX BioAnalytics using a commercially available platform (NucleoMag® VET Kit; Macherey-Nagel GmbH & Co. KG, Düren, Germany).
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bioRxiv - Microbiology 2020Quote: ... the soluble fraction was directly loaded onto a 1-ml nickel column (Protino Ni-nitrotriacetic acid [NTA]; Macherey-Nagel). The column was washed with Wash Buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... Products were then run on a 1% agarose gel and the gel was extracted using NucleoSpin (Macherey-Nagel, 740588.50) clean up kit according to manufacturer specifications.
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bioRxiv - Molecular Biology 2024Quote: ... DNA in the supernatant was precipitated with isopropanol and suspended in 250 μL of STE (10 mM Tris-HCl [pH 8.0], 1 mM EDTA [pH 8.0], 100 mM NaCl containing 80 μg/mL RNaseA [Macherey-Nagel]). RNA was digested by incubation for 1 hr at 37°C ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from the youngest leaf tissues of three biological replicates of DM-susceptible and DM-resistant yellow maize inbred lines (mock-inoculated and DM-inoculated) using the modified NucleoSpin™ Mini Kit for RNA purification (Macherey-Nagel™, GmbH & Co. Du ren, Germany). One hundred fifty (150 ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from 1 ml of crude homogenate containing 6 μl of proteinase K (22 mg/ml, Macherey-Nagel), incubated overnight at 55°C.
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected onto an RP18 column (4 × 50 mm, 2.7 μm particle size, Nucleoshell, Macherey-Nagel) with a flow rate of 0.25 mL min−1 ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from around 5×105 to 1×106 cells using the NucleoSpin RNA Plus (MACHEREY-NAGEL, 740984.50) or Quick-RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Plant Biology 2023Quote: ... from 2 to 3-day-old WT and mea-1-/-;dme-2-/- seedlings using NucleoSpin® Plant II (MACHEREY-NAGEL). 200 ng of DNA was subjected to fragmentation using ultrasonicator (Covaris ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were analyzed on 1% agarose gel and purified by NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel). 5’ and 3’ homology arms and the insert were cloned into the pUC19 vector (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... Harvested bacteria were lysed using a French press (3 runs at 20.000 psi) in 1× LEW buffer (Macherey-Nagel, Düren, Germany) containing 5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... In the following steps each amplicon generated either by PCR amplification or vector digestion was isolated and extracted from a 1% agarose gel through the NucleoSpin Gel (Macherey-Nagel) and PCR Clean-Up Kit (Takara ...
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bioRxiv - Cell Biology 2019Quote: ... The lipid was then dissolved in the 1-butanol and separated by two dimension high performance thin layer chromatography (NanoSiL, MACHEREY-NAGEL). For the first dimension ...
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bioRxiv - Developmental Biology 2024Quote: ... The His6-3C protease was then removed from the sample by immobilized metal affinity chromatography using a 1 mL Protino Ni-NTA column (Macherey-Nagel). The mTRIM66 PHD-bromo-domain protein was further purified by size exclusion chromatography using a HiLoad 16/600 Superdex 75 pg column (Cytiva ...
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bioRxiv - Genomics 2024Quote: ... dissolved in TE buffer (10 mM Tris pH8, 1 mM EDTA pH8) and purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel). DNA was loaded on a 2% agarose gel ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting bottom organic phase was dried and on-line derivatized to fatty acid methyl ester by a chloroform/methanol (1:2)-TMSH solution (Macherey-Nagel) and analyzed by gas chromatography-mass spectrometry (Agilent 5977A-7890B) ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The digestion reaction was run on a 1% agarose gel followed by excision of the digested band and purification via NucleoSpin columns (Macherey-Nagel). 100 ng digested pMB1-10x and 2.4 ng amplified sgRNA library insert were assembled using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was separated from the debris by centrifugation at 4000g for 40 minutes at 4°C and the supernatant was purified using 1 ml Ni-NTA columns (Macherey-Nagel). His-tagged UL12 was eluted using lysis buffer with increasing concentration of Imidazole (100 mM ...
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bioRxiv - Microbiology 2024Quote: ... was performed either when all ammonium (or nitrate) and nitrite was depleted, or when the values stayed constant for 1-3 days (qualitatively, using Quantofix (Macherey-Nagel) strips) ...
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bioRxiv - Biochemistry 2024Quote: ... After cell lysis using a French press the cell debris was removed by centrifugation at 17000 g for 1 h at 4 °C and the cleared lysate was incubated with Protino IDA resin (Macherey-Nagel) for 20 min at 4° C ...
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bioRxiv - Plant Biology 2021Quote: ... The aqueous phase was transferred to a new tube and incubated with 1 μL of RNase A (Macherey-Nagel, 12mg/mL stock) for 30 min at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared from cell debris by centrifugation at 35.000 g for 30 min and incubated for 1 hour with 2 g Ni-IDA matrix (Protino; Macherey-Nagel, Düren, Germany). The matrix was washed extensively with buffer L and bound protein was eluted in buffer L containing 250 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: The final PCR amplified library was submitted to two consecutive 1× NucleoMag NGS Clean-up and Size select magnetic bead selections (Macherey-Nagel – 7449970.5) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 3 mice at each time point (ZT 1, 5, 9, 13, 17, 21) were sacrificed and RNA was extracted (Macherey-Nagel, Oensingen, Switzerland) according to manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: OMM bacteria were grown as described and 1 mL of each bacterial culture supernatant was homogenized using NucleoSpin Bead Tubes (Macherey-Nagel, Düren, Germany) and a Precellys Evolution Homogenizer (Bertin Corp. ...
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bioRxiv - Genetics 2023Quote: ... and then incubated for 2 h on ice on a rocking platform with 1 ml Protino Ni-NTA Agarose (MN, Macherey-Nagel GmbH, Düren, Germany) pre-equilibrated with lysis buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we measured the chemical oxygen demand (COD) using NANOCOLOR COD tube tests (detection range 1-15 g/l by Macherey-Nagel (ref: 985 038), see (32 ...
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bioRxiv - Bioengineering 2022Quote: ... The remaining cuticular wax-chloroform mixture was filtered (cellulose folded filter paper; 4-12 µm pore size, M&N 615 1/4, Ø 185 mm, Macherey-Nagel GmbH & Co. KG), and chloroform was evaporated (rotary evaporator ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These were obtained by using as template a 1:10 serial dilution (8 dilutions) of a cleaned (Macherey-Nagel’s NucleoSpin Gel and PCR Clean-up) and quantified (Invitrogen’s Qubit™ ...