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Citations for Macherey-Nagel GmbH :
2201 - 2250 of 2271 citations for Rat Heme Oxygenase 1 HMOX1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted from 100 freshly harvested seeds in three biological replicates using the NucleoSpin® RNA Plant and Fungi kit (Macherey-Nagel, Düren, Germany), according to the manufacturer instructions ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and small (<200 nucleotides) and large (>200 nucleotides) RNA were extracted separately for each tissue with the NucleoSpin® miRNA kit (Macherey-Nagel; Düren, Germany). RNA quality and quantity were determined using a NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from tobacco leaf disks sampled from T1 plants and quickly frozen in liquid nitrogen using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Düren, Germany). cDNA was synthesized using 1 µg of total RNA according to the protocol in the RevertAid Reverse Transcriptase kit (Fermentas ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from ACE-grown cells (DSMZ medium 461 with 5 mM ACE) with the NucleoSpin® Microbial DNA kit (Macherey-Nagel, Düren, Germany) according to the manual and using wide-bore tips ...
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bioRxiv - Microbiology 2022Quote: ... The product of barcoding qPCR was cleaned by gel extraction using NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL, GmbH & Co, KG, Germany), after 1% AGE electrophoresis run for 1 hour at 100 V ...
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bioRxiv - Cell Biology 2024Quote: ... RNAlater was removed then tissues were transferred into screw-capped tube pre-filled with 350 µl of RA1 buffer of NucleoSPin RNAXS kit (Macherey-Nagel Cat# SKU: 740902.250). Tissues were homogenised with 10 pcs of 3 mm Acid-Washed Zirconium Beads (OPS diagnostics Cat# BAWZ 3000-300-23 ...
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bioRxiv - Zoology 2024Quote: ... Genomic (g)DNA was extracted and purified using the NucleoSpin®Tissue kit following the manufacturer’s instructions (Macherey-Nagel Gmbh & Co. KG, Düren, Germany). Primers used for the detection of all pathogens are found in Supplementary Data 3.
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bioRxiv - Genetics 2024Quote: RNA was extracted from a total of 24 samples of male brain (four vgll3*EE and four vgll3*LL individuals for each of the three time points) using a NucleoSpin RNA kit (Macherey-Nagel GmbH & Co. KG). The collected samples were transferred to tubes containing 1.4 mm ceramic beads from Omni International ...
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bioRxiv - Microbiology 2023Quote: ... inoculated seeds and seedlings of the SynCom conditions (control, 6-strain, 8-strain, 12-strain SynComs) with the NucleoSpin® 96 Food kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... a 450 bp long sequence upstream of timA was synthesized by PCR from EGD-e genomic DNA using primers TE74 and TE75 and purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). For the EMSA experiment ...
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bioRxiv - Synthetic Biology 2023Quote: ... Base-editing efficiency when targeting a plasmid-borne gene was estimated by isolating plasmid DNA upon 24-h editing treatment by using the NucleoSpin™ plasmid EasyPure kit (Macherey-Nagel, Dueren, Germany). Either the isolated plasmids or the purified PCR products from independent samples were Sanger-sequenced using the Mix2Seq™ kit (Eurofins Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was isolated from peripheral blood and tail tissues of each chimeric mouse 6 weeks after BM transplantation with NucleoSpin® Tissue Kit (MACHEREY-NAGEL, Duren, Germany). Then ...
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bioRxiv - Systems Biology 2023Quote: ... The pellet was washed once with sterile H2O and then each library was purified over two columns of the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany). A total of 17 Mio were harvested ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA extractions were either performed with a modified CTAB 2%/PVPP 2% protocol (see Jolly et al. 2003) or the NucleoSpin®Tissue 96 kit (Macherey-Nagel, Karlsruhe, Germany).
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bioRxiv - Plant Biology 2024Quote: ... Maize leaf discs sampled at 4 dpi were homogenized using metal beads in a Retsch mixer mill and fungal genomic DNA was extracted with NucleoSpin® Plant II Kit (Macherey-Nagel, Dueren, Germany) as explained before ...
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bioRxiv - Genomics 2024Quote: ... tissues were immediately frozen in liquid nitrogen and stored at -80°C prior to total RNA isolation with NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel Inc., PA, US). RNA libraries for RNA-Seq were prepared using the Illumina TruSeq Stranded Total RNA Ribo-Zero Plant kit (Illumina ...
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bioRxiv - Genetics 2024Quote: ... using Ion Xpress™ Plus Fragment Library Kit (Thermo Scientifc, USA) and NucleoMag® NGS Clean-up and Size Select beads (Macherey-Nagel, Germany) following manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and bands of the correct size were excised and cleaned up with a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Cat. No. 740609.5) followed by cleanup with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2024Quote: ... PCRs for loci of interest were run using Amplicon-EZ (Genewiz) partial IlluminaÒ adapters and amplicons were processed using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Catalog no. 740609.250). Paired end (2 x 250 bp ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was then loaded on a Protino® GST/4B column 1 ml (Macherey-Nagel) for pull-down experiments ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µl from each sample was spotted onto a POLYGRAM CEL 300 PEI-TLC plate (Macherey-Nagel), and developed with 0.5 M LiCl (Sigma) ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The PCR product (325 bp) was purified from a 1% agarose gel via NucleoSpin columns (Macherey-Nagel). NGS was performed on an Illumina Miniseq using a MiniSeq Mid Output Kit (300-cycles ...
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bioRxiv - Microbiology 2024Quote: ... 4°C) and the supernatant was passed through a 1 ml Ni-NTA column (Protino, Macherey-Nagel) utilizing liquid chromatography (ÄKTApurifier) ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from tobacco leaf disks (sampled from glasshouse grown plants and quickly frozen in liquid nitrogen) using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Fisher Scientific, UK). cDNA was synthesized using 1 μg total RNA in 20 μl using the oligo-dT primer according to the protocol in the RevertAid Reverse Transcriptase kit (Fermentas ...
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bioRxiv - Developmental Biology 2022Quote: ... All plasmids were cloned into the DH5a strain (Enzynomics) and purified with a NucleoBond Xtra Midi Plus kit (MACHEREY-NAGEL GmbH & Co. KG, Dueren, Germany) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: RNA from VTMs or swab-immersed TE buffer or cell culture supernatant was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG; 740956.250). The SARS-CoV2 RNA was quantified using (LabGun™ COVID-19 RT-PCR Kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The leaf material was lysed with sterile sand and 7mm glass beads before DNA was extracted with the NucleoMag 96 plant kit (Macherey-Nagel Gmbh & Co., Düren, Germany) using the KingFisher Flex magnetic particle processor (Thermo Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... and the 3’-terminal poly A) were amplified by PCR and purified using the NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Then we co-extracted the total RNA and DNA of the biological replicates on up to 0.35 g frozen soil sample with the NucleoBond RNA Soil Mini kit (Macherey-Nagel GmbH & Co. KG Dueren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from 12-day-old plantlets grown in vitro using the RNA Plus Kit (Macherey-Nagel GmbH & Co. KG, Düren, NRW, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid preparation and DNA gel extraction were performed using the Nucleospin Plasmid and the Nucleospin Extract II kits (Macherey-Nagel, Lab Supplies Scientific SA, Hellas). Restriction enzymes were from Takara Bio (Lab Supplies Scientific SA ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were separated on 2.5% agarose gels and extracted using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany, cat. no. 740609). The purified PCR products were then cloned using a Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplified regions of CRISPR-C unedited samples and the specific bands derived from the amplification of the deletion scar and circular junction sites were dissected and DNA products purified by NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL Düren, Nordrhein-Westfalen, Germany) and sent for Sanger sequencing at Eurofins Genomics ...
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bioRxiv - Evolutionary Biology 2020Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean‑up (Macherey-Nagel, Düren, Germany). Following digestion with restriction enzymes ...
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bioRxiv - Evolutionary Biology 2019Quote: The pH of individual reaction mixtures was determined via TRITEST L pH 1–11 pH papers (Macherey-Nagel) directly after the reaction ...
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bioRxiv - Biochemistry 2022Quote: ... Table 1) followed by column purification using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). After sequencing the purified PCR product ...
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bioRxiv - Microbiology 2019Quote: For the hybrid LAMP assay total DNA was extracted from two or three frozen cedar baits using the Machery-Nagel Plant Kit II (Macherey-Nagel GmbH & Co. KG, Düren, Germany) and the manufacturer’s recommended protocol for plant material ...
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bioRxiv - Microbiology 2022Quote: Swab tips were excised using sterile scissors and were subject to DNA extraction using the NucleoSpin Soil DNA extraction kit (Macherey-Nagel Inc., Allentown, PA; catalogue: 740780) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... containing 1819 adenine bases) was amplified by PCR and purified using a NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) with Milli-Q water ...
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bioRxiv - Biochemistry 2023Quote: ... and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). In vitro methylation experiments were performed for 10 min (DNA ...
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bioRxiv - Biochemistry 2023Quote: ... and purified in RNase-free water using a NucleoSpin RNA Clean-up spin column kit with a DNA removal column (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). In vitro RNA methylation experiments were performed for 2 h at 37 °C in 20 μL reaction mixtures containing 1× CutSmart buffer (New England Biolabs Inc. ...
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bioRxiv - Plant Biology 2023Quote: ... RNA were extracted at different germination timepoints and in different tissues using the NucleoSpin® RNA Plant and Fungi Kit (Macherey Nagel, DuiJren, Germany) as described above but including a DNAse treatment (Macherey-Nagel, rDNAse set, DuiJren, Germany). RNA were quantified using a using a NanoDrop ND-1000 (NanoDrop Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Total nucleic acids were extracted from mouse samples submitted to IDEXX BioAnalytics using a commercially available platform (NucleoMag® VET Kit; Macherey-Nagel GmbH & Co. KG, Düren, Germany).
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bioRxiv - Microbiology 2020Quote: ... the soluble fraction was directly loaded onto a 1-ml nickel column (Protino Ni-nitrotriacetic acid [NTA]; Macherey-Nagel). The column was washed with Wash Buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... Products were then run on a 1% agarose gel and the gel was extracted using NucleoSpin (Macherey-Nagel, 740588.50) clean up kit according to manufacturer specifications.
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bioRxiv - Molecular Biology 2024Quote: ... DNA in the supernatant was precipitated with isopropanol and suspended in 250 μL of STE (10 mM Tris-HCl [pH 8.0], 1 mM EDTA [pH 8.0], 100 mM NaCl containing 80 μg/mL RNaseA [Macherey-Nagel]). RNA was digested by incubation for 1 hr at 37°C ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from the youngest leaf tissues of three biological replicates of DM-susceptible and DM-resistant yellow maize inbred lines (mock-inoculated and DM-inoculated) using the modified NucleoSpin™ Mini Kit for RNA purification (Macherey-Nagel™, GmbH & Co. Du ren, Germany). One hundred fifty (150 ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from 1 ml of crude homogenate containing 6 μl of proteinase K (22 mg/ml, Macherey-Nagel), incubated overnight at 55°C.
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected onto an RP18 column (4 × 50 mm, 2.7 μm particle size, Nucleoshell, Macherey-Nagel) with a flow rate of 0.25 mL min−1 ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from around 5×105 to 1×106 cells using the NucleoSpin RNA Plus (MACHEREY-NAGEL, 740984.50) or Quick-RNA Miniprep Kit (Zymo Research ...