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Citations for Macherey-Nagel GmbH :
2001 - 2050 of 2132 citations for Creatinine Urinary Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from microglia after CD11b microbead isolation from P7 neonates born to and nursed by WT and Slc2a5−/− dams using the NucleoSpin RNA kit (Macherey-Nagel, 740955.250). The QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were grown in Luria-Bertani Broth at 37°C and plasmid DNA was isolated using the Nucleobond Xtra midi kit (MACHEREY-NAGEL, 740410), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products from the same sample were pooled and purified over 2% agarose gels (band size at 275 bp) using NucleoSpin® Gel and PCR clean up kit (Macherey-Nagel). Purified amplicons were quantified by Qubit (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA gene of Ca ...
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bioRxiv - Cancer Biology 2023Quote: ... or 1.2 l LB-medium and incubated for 4 h at 37 °C before extracting plasmid DNA using Nucleobond Xtra Midi kit (Macherey-Nagel, C640003) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2023Quote: Genomic DNA of the 54 genotypes was extracted from the leaves using a mixed alkyltrimethylammonium bromide buffer and the kit NucleoMag Plant (Macherey-Nagel, Germany). DNA samples were quantified using ThermoFisher Scientific fluorometry Qubit ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from INS-1 832/13 cells 48h after transfection using the NucleoSpin extraction kit (Macherey-Nagel, Düren, Germany). The amount of isolated RNA was measured using the NanoDrop system ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from 8–12 mg of dried tissue using the NucleoSpin 96 Plant II kit (Macherey-Nagel GmbH & Co. KG) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting constructs were used for protoplast transformation through midiprep using the NucleoBond Xtra plasmid kit (Macherey-Nagel GmbH & Co.KG, Duren, German) as per the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Both gel purifications and PCR clean-ups were performed using the NucleoSpin® PCR and Gel Clean Up Kit (Macherey-Nagel, Germany). DNA concentrations and purities were measured on a NanoDropTM 2000 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Systems Biology 2023Quote: ... 700ng of the plasmid library was isolated from the liquid culture using the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Genomics 2023Quote: Perca fluviatilis gDNA samples from 35 males and 35 females were extracted with the NucleoSpin Kit for Tissue (Macherey-Nagel, Duren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... consecutive copies of 197 bp with modified 601 sequence were transformed into DH5α Escherichia coli strain and purified from four liters of culture by using plasmid DNA purification kit Nucleobond® PC 10000 (Macherey-Nagel). Three mg of the plasmids were digested with EcoRI HF (0.25 U/µg DNA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The total RNA of the brain samples was extracted according to the manufacturer’s protocol by applying the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The quality and quantity of RNA were assessed at 260 nm using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of the sample with gRNA–TAR and –p24 combined was run on a 1% agarose gel containing ethidium bromide and the two expected bands were cut out separately and purified using the Nucleospin® Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s manual ...
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bioRxiv - Evolutionary Biology 2023Quote: Bulk DNA of the meristematic region and the basal portions of the leaves was extracted using NucleoSpin Plant II kit (Macherey-Nagel, Germany). DNA concentration was determined using a Qubit Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... were then extracted from the agarose gel and the DNA purified following the manufacturer instruction (NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL)) ...
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bioRxiv - Molecular Biology 2023Quote: ... these cultures were incubated overnight (800 RPM, 37°C) and miniprepped the following day using a 96 Plasmid NucleoSpin kit (Macherey-Nagel #740625.1). The resulting plasmids were able to be sequenced verified for proper VH and VL insertion ...
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bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from six-day-old seedlings (approximately 20 seedlings per genotype) using the NucleoSpin RNA plant kit (Macherey-Nagel CO.). Subsequently ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The isolation of gDNA was conducted either by using a column-based method following manufacturer’s instructions (NucleoSpin Microbial DNA kit, Macherey-Nagel, Düren, Germany) or an automated system (King Fisher Duo Prime System ...
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bioRxiv - Cancer Biology 2023Quote: ... the cultured alone C4-2B cells or the mixed co-cultured C4-2B/MC3T3-E1 were harvested for DNA extraction using the NucleoSpin Tissue kit (Macherey-Nagel Inc.). Primers against the nuclear and mitochondrial genomes were used for qRT-PCR (sequences provided in Supplementary Table S2 ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR reaction was performed on the new synthetized vector to amplify the HA1-DCK*-P2A-GFP-HA2 sequence corresponding to the HDR (homology directed repair) DNA template and was purified using the NucleoSpin PCR Clean-up kit (Macherey-Nagel # 740609.10) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2024Quote: ... RNA from 150 µl of excreta solution was extracted by adding 600 µl of RAV1 lysis buffer and using NucleoSpin virus RNA kit (Macherey-Nagel, France).
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bioRxiv - Zoology 2024Quote: ... total RNA was extracted from the second pooled sample of 60 bees using a NucleoSpin®RNA kit (Macherey-Nagel Gmbh & Co.), cDNA was synthesized from 800 ng total RNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories) ...
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bioRxiv - Developmental Biology 2024Quote: ... The three constructs were then cultured for 24 h at 37°C and purified with NucleoBond Xtra Midi Kit (Macherey-Nagel, Germany) and used for gene reporter assay.
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... and we collected bands containing arrays ≥2-plex at 550-650 bps and 650-800 bps to be purified separately using a NucleoSpin Gel Purification Kit (Macherey-Nagel 740609.250). We also collected the cut vector backbone ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting amplicons were purified using the NucleoSpin gel and PCR cleanup kit following the manufacturer’s instructions (Macherey-Nagel Inc., Bethlehem, PA) and submitted for Sanger sequencing with the same primers used to generate the amplicons ...
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bioRxiv - Microbiology 2023Quote: ... The dephosphorylated pEX18Tc vector and PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). TEDA reaction was then carried out by mixing 150 ng of pEX18Tc with the corresponding PCR products at a molar ratio of 1:4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The NaOH was neutralized by the addition of 1 M HCl and the cDNA was purified by a PCR-clean-up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Germany) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to pellet the cell debris and the RNA was extracted from the supernatant using the two column-system of the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Germany). DNA removal was ensured by the on-column DNA digestion according to the manufacturer’s description ...
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bioRxiv - Genetics 2022Quote: ... DNA was then extracted from tissue using the NucleoSpin Tissue Mini kit columns and corresponding protocol for extraction from animal tissue (Macherey-Nagel, Duren, Germany). For each sperm pool ...
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bioRxiv - Microbiology 2019Quote: Environmental DNA was extracted from 0.3 g of crushed rocks using NucleoSpin® Plant II Kit (Macherey-Nagel, Gmbh & Co. KG, Duren, Germany) according to the manufacturer’s instructions and quantified by Quant-iT dsDNA HS assay kit (Invitrogen molecular probes-Eugene ...
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bioRxiv - Cancer Biology 2020Quote: ... GRANTA-519 and B-cells purified from four MCL patients using the NucleoSpin® RNA II kit according to the manufacturer’s protocol (Macherey-Nagel, Oensingen, Switzerland). A minimum of 2µg per samples were used for analysis ...
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bioRxiv - Biochemistry 2019Quote: Genomic DNA was isolated using the NucleoSpin® RNA II and NucleoSpin® RNA/DNA Buffer Set kit (MACHEREY-NAGEL, Düren, Mannheim, Germany) kit according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... were extracted from fresh haploid mycelium after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel, Düren, Germany). Sequencing was performed using the Illumina HiSeq 2500 paired-end technology (Illumina Inc. ...
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bioRxiv - Genomics 2019Quote: ... placed in 90% ethanol and then stored dried until gDNA extraction was performed using the NucleoSpin Kit for Tissue (Macherey-Nagel, Duren, Germany). Genomic DNAs from individual fish were then quantified using a Qubit fluorometer (Thermofisher ...
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bioRxiv - Cancer Biology 2019Quote: ... genomic DNA was isolated from the peripheral blood and tail tissues of each chimeric mouse 30 days after BM transfer using a NucleoSpin tissue kit (Macherey-Nagel, Duren, Germany). Then ...
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bioRxiv - Neuroscience 2020Quote: ... The total proteins were precipitated and prepared for Polyacrylamide Gel Electrophoresis using protein precipitator and resuspension buffer PSB/TCEP (Protein solving buffer and (tris(2-carboxyethyl)phosphine) TCEP reducing agent) from the NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France). The elution fraction containing RNA was further used for PCR analysis (see below) ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated from leaves harvested at the indicated time point using the NucleoSpin® RNA Plus kit (Macherey-Nagel, Düren, Germany). cDNA was synthesized from 2 μg of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Plant Biology 2021Quote: Extractions of total genomic DNA were generated from silica gel-dried leaf material using the NucleoSpin Plant II kit (Macherey-Nagel, Dueren, Germany) or from herbarium specimens using the CTAB DNA isolation method as modified by Borsch et al ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted plant DNA of at least one diseased plant individual per site using the NucleoSpin® 96 Plant II kit (Macherey-Nagel, Germany). We obtained DNA for 134 out of the 171 diseased plant individuals ...
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bioRxiv - Bioengineering 2019Quote: ... genomic fragments containing the gRNA target site were amplified with PCR and purified using NucleoSpin Gel and a PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Then 200 ng of each of the PCR products derived from the transfected and non-transfected cells were denatured at 95° C for 5 min and reannealed ...
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bioRxiv - Plant Biology 2021Quote: ... The amplicons were purified from the PCR reagent tube via the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Each worm pellet was re-suspended in 1M DTT and RA1 (solution from the NucleoSpin® RNA kit (Macherey-Nagel, Duren Germany #740955.50)) and frozen at −80°C overnight ...
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bioRxiv - Plant Biology 2022Quote: Leaves from 6- to 8-week-old Vicia faba plants were harvested and mesophyll RNA was extracted using the NucleoSpin Plant RNA extraction kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: The genomic DNA of Rhodococcus strain IGTS8 was isolated using the NucleoSpin Tissue DNA Extraction kit (Macherey-Nagel, Lab Supplies Scientific SA, Hellas) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... was linearized by AdeI restriction enzyme digestion and DNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). For in-vitro transcription ...
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bioRxiv - Molecular Biology 2022Quote: RNA from cell culture supernatant after CPZ/PCZ treatment (mentioned above) was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG). Real-time quantitative RT-PCR was performed in Roche LightCycler 480 using commercial kits ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 35 mg of animals were transferred into a 1.5 mL tube and total RNA was extracted using a Nucleospin® RNA Plus Kit (Macherey-Nagel, Düren, Germany). The prey cDNA library was screened for interactions with the 70 distal C-terminal amino acids of the TadNaC2 and TadNaC10 channels expressed in yeast from the bait vector pGBK-T7 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the samples were frozen and stored at −80 °C until the total RNA was extracted using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany). The transcriptome was analyzed for three replicates from independent runs of the exposure experiment ...