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Citations for Macherey-Nagel GmbH :
151 - 200 of 247 citations for Nonanoic acid reaction products with diethanolamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The products were inspected on agarose gel and purified using Nucleofast PCR purification plates (Macherey-Nagel, Dueren, Germa) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and the resultant PCR product gel extracted using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). flaB promoter-lacI and pQE30 were Q5 PCR-amplified from pJSB252 using primers PA468 + PA471 (for pind template ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments and PCR products were purified using the Nucleospin Gel and PCR clean up kit (Macherey-Nagel).
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were evaluated by electrophoresis and then further processed with the PCR clean-up Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and the reverse transcription products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). The reverse transcription products were eluted with 30 μL of HiDi formamide (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified by a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel., Düren, Germany). COI gene fragments were amplified from some samples according to the USDA recommended protocol (https://www.fsis.usda.gov/sites/default/files/media_file/2020-11/CLG-FPCR.pdf ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were subsequently purified using the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... PCR product fragments were excised from gels and purified with a PCR clean-up kit (Macherey-Nagel Nucleospin).
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bioRxiv - Immunology 2024Quote: ... After purification of the RT-PCR products using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... RT-PCR products were purified with NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Carlo Erba, Italy). Sequencing libraries were made with a Nextera-XT DNA Library Prep Kit (Illumina Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting PCR product was gel purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and 3’ A-overhangs were added by incubation with Taq-polymerase (DreamTaq Green PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA for cell-free reactions was prepared from overnight bacterial cultures using Maxiprep kits (Macherey-Nagel).
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bioRxiv - Molecular Biology 2023Quote: ... were purified from the reaction with NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel) according to the manufacturer’s instructions and subjected to a T7 Endonuclease assay (T7EI ...
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were monitored by thin layer chromatography (TLC, silica gel 60, UV254, Macherey-Nagel, ref: 818333) and compounds were visualized by UV absorption (254 nm and/or 366 nm ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the PCR products were purified using a NucleoSpin Gel and a PCR Clean-up Kit (Macherey-Nagel, Duren, Germany) and then sequenced directly by Sanger sequencing using the same PCR primers ...
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bioRxiv - Genomics 2021Quote: ... PCR was performed and the products were purified with a NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL).
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bioRxiv - Biophysics 2022Quote: ... Products were then run on a 1% agarose gel and the gel was extracted using NucleoSpin (Macherey-Nagel, 740588.50) clean up kit according to manufacturer specifications.
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the adeD PCR product was additionally gel-purified (NucleoSpin Gel and PCR Clean-Up Kit, Macherey-Nagel, Düren, DE), and 100 ng of purified DNA was subsequently incubated with 0.2 M dATP and 5 U DreamTaq Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Resulting PCR products were purified from 1% agarose gels with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel) to prevent the production of side products during in vitro transcription ...
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bioRxiv - Molecular Biology 2022Quote: ... Certain PCR products were purified from the gel using NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel) and cloned individually in a TA-cloning vector called pGEM®-T Easy Vector (Promega ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR product was purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel Cat#740609.50), and resolved on a 8% polyacrylamide TBE gel (Life Technologies Cat#EC62162BOX) ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR clean-up was performed on the correct RT-PCR products according to the manufacturer’s instructions (Macherey-Nagel, #740609.250). 4-6 ng of each product were sent for Sanger sequencing to Eurofins Genomics Germany GmbH.
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were separated by agarose gel electrophoresis and purified from agarose gels using the NucleoSpin kit (Macherey-Nagel). Purified PCR products were subcloned into linearized plasmid vectors of interest using the In-fusion cloning system following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: PCR products from nematode specimens were purified using a NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Germany). Nucleotide sequences were determined by direct sequencing using a BigDye Terminator Kit ver ...
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bioRxiv - Genetics 2024Quote: ... PCR products were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Dü ren, Germany) and send to Eurofins Genomics for Sanger sequencing of both strands ...
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bioRxiv - Genomics 2021Quote: ... PCR reactions were pooled and purified on NucleoSpin Gel and PCR Clean-up #740609 columns (Macherey-Nagel). The screening vector was linearized with AgeI-HF and SalI-HF restriction enzymes (NEB ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used for cloning reactions were purified by using the Nucleospin gel extraction kit (Macherey-Nagel) according to the manufacturer’s specifications ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR reaction was purified with the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Germany) according to the manufacturer’s protocol and the purified fragments were eluted in 20 µL of RNase-free H2O ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were purified to remove unincorporated nucleotides and primers using NucleoFast® 96 PCR plates (Macherey-Nagel, Oensingen, Switzerland). Details of the annealing temperatures and primers were published previously (Friesen et al ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were cleaned up using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel, 740609.50) and sequenced using Sanger Sequencing (Department of Biochemistry ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were cleaned up using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel, 740609.50) and sent for Sanger Sequencing (Department of Biochemistry ...
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bioRxiv - Bioengineering 2021Quote: ... Unincorporated PCR primers and dNTPs were removed from the PCR products using the Nucleospin PCR clean-up kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected by gel electrophoresis on 2% Agarose/TAE and gel extracted by column purification (Macherey-Nagel). Amplicons were paired-end sequenced with 500 cycles on a MiSeq system (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were size-selected for 400-550 bp using a 2% agarose/TAE gel and column purification (Macherey-Nagel). Libraries were sequenced as above ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were subjected to a final gel purification using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel).
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bioRxiv - Biophysics 2022Quote: ... The product was then purified from 1% agarose gel and extracted using the NucleoSpin clean up kit (Macherey-Nagel, 740588.50).
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bioRxiv - Biochemistry 2023Quote: ... Digestion products were isolated on agarose gels and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Plasmid isolation was performed with NucleoSpin Plasmid kit (Macherey-Nagel) ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR products were purified using the NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, catalog no. 740609.50) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were analyzed on 1% agarose gel and purified by NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel). 5’ and 3’ homology arms and the insert were cloned into the pUC19 vector (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were purified using DNA gel extraction with NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL, 740609.10). Transformation was performed with purified PCR products in Stellar Competent Cells (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription products were purified by NucleoSpin Gel and PCR clean-up kit equilibrated with NTC buffer (Macherey-Nagel). Dideoxy DNA sequencing samples were prepared using Thermo Sequenase DNA polymerase (Cytiva ...
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting PCR product was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel GmbH, Cat. # 740609.50), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used in cloning reactions were purified using the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... PCR reactions were purified using a PCR/Gel purification kit (NucleoSpin Gel and PCR Clean□up, Macherey-Nagel).
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bioRxiv - Genetics 2021Quote: ... Nucleic acid extraction was performed with the NucleoSpin DNA Insect Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The amplified products were identified by gel electrophoresis and purified using Nucleospin PCR and Gel Clean Up Kit (Macherey-Nagel # 740609.5). Alternatively ...
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bioRxiv - Microbiology 2021Quote: ... Both PCR products were excised from a 1% agarose gel and extracted using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) and used as templates for In-Fusion HD cloning (Takara) ...
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bioRxiv - Developmental Biology 2021Quote: ... and the reverse primer 5’AACCCAGGTAATGAACACAGTTTCTAT. PCR products were run on a gel (supplemental Fig. 2B) and bands were gel purified (Macherey-Nagel), inserted in a pCR-bluntII-Topo vector (Invitrogen ...