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Citations for Macherey-Nagel GmbH :
151 - 200 of 224 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... All plasmids were purified from 4 ml bacterial culture using either NucleoSpin® Plasmid (REF 740588.50, Macherey-Nagel®) or PureLink™ HiPure Plasmid Miniprep Kit (Art ...
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bioRxiv - Microbiology 2022Quote: ... For Clr purification the initial purification was conducted using a 5 mL Protino™ Ni-NTA Column (Macherey-Nagel). Size-exclusion chromatography over a 16/600 Superdex 200 column (GE ...
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bioRxiv - Microbiology 2023Quote: ... Small RNA was isolated from AW-EVs or ML-EVs pellets using NucleoSpin® miRNA (MACHEREY-NAGEL, Duren, Germany). The small RNA library was prepared using NEBNext Small RNA Library Prep Set for Illumina (New England Biolaps ...
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bioRxiv - Immunology 2023Quote: ... Total RNA (0.2–0.4 mL) of mouse whole blood was isolated using NucleoSpin RNA Blood (Macherey-Nagel GmbH & Co.) according to the manufacturer’s instructions with on-column DNA digestion ...
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bioRxiv - Microbiology 2023Quote: ... slight adaptations were made from manufacturer’s instructions: half MCE filters were placed into individual 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel) and added with 1,200 µL MWA1 lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 4°C and the supernatant was incubated at 4 °C with 1.25 ml Ni-NTA agarose resin (Macherey-Nagel) for 1 h with agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... treatment with 100µg/ml RNase A (37° for 15 minutes) and 50µg/ml proteinase K and purification using the PCR cleanup kit (Macherey-Nagel).
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bioRxiv - Microbiology 2021Quote: ... as well as cKD TgISU1 (incubated with or without ATc at 1.5 μg/mL for 3 days) were extracted using Nucleospin RNA II Kit (Macherey-Nagel). The cDNAs were synthesized with 450 ng of total RNA per RT-PCR reaction using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Genomics 2022Quote: ... Midipreps for all constructs were prepared at final concentration >1mg/mL using the NucleoBond Xtra Midi Plus kit (Macherey-Nagel).
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bioRxiv - Bioengineering 2022Quote: ... containing an insert (15 mm tip, wide opening, 0.2 mL, 6×31 mm, clear, Macherey-Nagel GmbH & Co. KG Germany) for High-Performance Liquid Chromatography (HPLC ...
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bioRxiv - Microbiology 2020Quote: ... Samples of 5 mL liquid culture were quickly harvested onto nitrocellulose membrane filters (ø 25 mm, 0.45 µm pore size, Porafil NC, Macherey-Nagel) by vacuum filtration ...
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bioRxiv - Microbiology 2021Quote: ... 107 cells were inoculated in 50 ml LB medium supplemented with 100 µg/ml spectinomycin and grown overnight at 37 °C for purification of the plasmid library DNA by miniprep (NucleoSpin Plasmid, Macherey-Nagel). The efficiency of fragment insertion (100% ...
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bioRxiv - Evolutionary Biology 2019Quote: ... one freshly opened flower was taken out of the plant at 20:00 and incubated with polydimethylsiloxane (PDMS) tubes in a sealed 8 mL glass vial (MACHEREY-NAGEL)33.
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were extracted from positive colonies using 5 mL liquid cultures with the NucleoSpin Plasmid Purification kit (Macherey-Nagel; Ref.: 11932392) and sequenced (GATC ...
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bioRxiv - Neuroscience 2023Quote: ... The positive clones were grown in 300 mL LB cultures and the plasmids were extracted using the NucleoBond Xtra Midi EF kit (Macherey-Nagel). The plasmids were sent out for sequencing again before being packaged into AAVs.
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bioRxiv - Cancer Biology 2023Quote: ... Plates were scraped with 30-50 mL Luria-Bertani (LB) broth and plasmids were purified with Endotoxin-Free MaxiPrep kit (Macherey-Nagel). Precipitated DNA was resuspended in 1 mL water and the concentration was measured at Nanodrop ...
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bioRxiv - Genomics 2021Quote: ... Menaquinones were then removed from the plate and analyzed using HPLC fitted with a reverse phase column (Macherey-Nagel, 2 mm x 125 mm, 3 µm, RP18) with methanol ...
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The complete mitochondrial genome of Spurilla braziliana MacFarland 1909 (Nudibranchia, Aeolidiidae)bioRxiv - Genomics 2023Quote: ... Japan (Figure 1) and preserved in NucleoProtect RNA (MACHEREY-NAGEL) for 5.5 months at -80 °C before DNA extraction ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was filtered using a 0.45 µm syringe filter and loaded onto a 5 ml Protino Ni-NTA column (Macherey-Nagel, Düren, Germany). The column was washed until UV280 had reached a steady value and eluted with 500 mM imidazole ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was diluted with 4.5 ml water and subjected to solid-phase extraction on HR-XC (Chromabond, Macherey-Nagel, Düren, Germany) column ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... bacteria from the cultures used for infection were immediately pelleted by centrifugation and resuspended in 8 mL of RES-EF of the NucleoBond Xtra Midi EF kit prior to proceeding with the standard kit protocol (Macherey-Nagel 740420.50). Precipitated vectors were resuspended in 120 µL of water.
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bioRxiv - Molecular Biology 2020Quote: ... The overnight culture was used to inoculate a 500 mL LB with the antibiotic and the plasmid DNA was isolated using NucleoBond Xtra Midi EF (Macherey-Nagel, Dueren, Germany). Sequence flanking the spacer sequences were amplified and were sequenced ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 35 mg of animals were transferred into a 1.5 mL tube and total RNA was extracted using a Nucleospin® RNA Plus Kit (Macherey-Nagel, Düren, Germany). The prey cDNA library was screened for interactions with the 70 distal C-terminal amino acids of the TadNaC2 and TadNaC10 channels expressed in yeast from the bait vector pGBK-T7 ...
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bioRxiv - Plant Biology 2019Quote: ... The cooled samples were filled up with deionized water to a volume of 25 ml and filtered through ashless filter paper (MN640 m, Ø125 mm, manufactured by Macherey-Nagel, Düren, Germany). Absorption was measured at 213.8 nm for Zn and 248.3 nm for Fe with an atomic absorption spectrometer (model 11003 ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from 1 × 106 cells using NucleoSpin DNA RapidLyse (MACHEREY-NAGEL). qPCR was performed with primers targeting the ND2 locus in the mitochondrial genome (5′-TGTTGGTTATACCCTTCCCGTACTA-3′ and 5′- CCTGCAAAGATGGTAGAGTAGATGA-3′ ...
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bioRxiv - Microbiology 2023Quote: ... 29-18-1 using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... PA14 and PAK from ∼1 × 108 CFU using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel), as per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Gibson assembly was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... Pre_S or FECD corneal endothelial tissues (Supplemental Table 1) by NucleoSpin RNA XS kit (Macherey-Nagel). cDNAs were prepared by reverse transcription ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM PMSF) by sonication followed by loading on to Ni-IDA column (Macherey-Nagel, Germany). Endonuclease G was eluted in gradient imidazole concentration (100-500 mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was first extracted from 1 x 105 cells using NucleoSpin Tissue (MACHEREY-NAGEL #740952) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2019Quote: ... RNA was recovered from 1 x 106 cells using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The RNA was sent to Eurofins for RNA-seq analysis ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µl from each sample was spotted onto a POLYGRAM CEL 300 PEI-TLC plate (Macherey-Nagel), and developed with 0.5 M LiCl (Sigma) ...
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bioRxiv - Genomics 2023Quote: ... was purified from a 1% agarose gel using a Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The PCR product (325 bp) was purified from a 1% agarose gel via NucleoSpin columns (Macherey-Nagel). NGS was performed on an Illumina Miniseq using a MiniSeq Mid Output Kit (300-cycles ...
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bioRxiv - Evolutionary Biology 2020Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean‑up (Macherey-Nagel, Düren, Germany). Following digestion with restriction enzymes ...
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bioRxiv - Evolutionary Biology 2019Quote: The pH of individual reaction mixtures was determined via TRITEST L pH 1–11 pH papers (Macherey-Nagel) directly after the reaction ...
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bioRxiv - Biochemistry 2022Quote: ... Table 1) followed by column purification using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). After sequencing the purified PCR product ...
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bioRxiv - Biophysics 2022Quote: ... Products were then run on a 1% agarose gel and the gel was extracted using NucleoSpin (Macherey-Nagel, 740588.50) clean up kit according to manufacturer specifications.
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bioRxiv - Molecular Biology 2022Quote: ... Resulting PCR products were purified from 1% agarose gels with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel) to prevent the production of side products during in vitro transcription ...
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bioRxiv - Molecular Biology 2024Quote: 1 µg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 µl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441) ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected onto an RP18 column (4 × 50 mm, 2.7 μm particle size, Nucleoshell, Macherey-Nagel) with a flow rate of 0.25 mL min−1 ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA constructs for mammalian tissue culture (Supplementary Table 1) were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from around 5×105 to 1×106 cells using the NucleoSpin RNA Plus (MACHEREY-NAGEL, 740984.50) or Quick-RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Biophysics 2022Quote: ... The product was then purified from 1% agarose gel and extracted using the NucleoSpin clean up kit (Macherey-Nagel, 740588.50).
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were analyzed on 1% agarose gel and purified by NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel). 5’ and 3’ homology arms and the insert were cloned into the pUC19 vector (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 0.25 g (3.6 g TSS L−1) activated sludge was processed to extract intracellular DNA using a NucleoSpin® Soil kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Harvested bacteria were lysed using a French press (3 runs at 20.000 psi) in 1× LEW buffer (Macherey-Nagel, Düren, Germany) containing 5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... In the following steps each amplicon generated either by PCR amplification or vector digestion was isolated and extracted from a 1% agarose gel through the NucleoSpin Gel (Macherey-Nagel) and PCR Clean-Up Kit (Takara ...