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Citations for Macherey-Nagel GmbH :
1801 - 1850 of 2218 citations for Vitamin A Retinol ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: Total RNAs were extracted from blood of 137 ewes with the Nucleospin® RNA Blood Kit (Macherey-Nagel, #Ref 740200.50) according to the manufacturer’s protocol starting with 800µL of whole blood with a DNAseI digestion treatment to eliminate contaminating genomic DNA ...
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bioRxiv - Neuroscience 2023Quote: We generated high-quality DNA of the expression constructs for electroporations using the NucleoBond Xtra Midi kit (Macherey-Nagel 740410.50)
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 200 mg of stool sample using a modified NucleoSpin® DNA Stool kit (Macherey-Nagel, Germany) via physical ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was extracted from the final pellets using the NucleoBond High Molecular Weight DNA Kit according to the manufacturer’s instruction (Macherey-Nagel).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The 140-bp UPTAG and DNTAG libraries were purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel), quantified using the Qubit dsDNA HS kit (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Digestion products were isolated on agarose gels and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Plasmid isolation was performed with NucleoSpin Plasmid kit (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2023Quote: Dissected thyroids were immediately placed in RNAlater solution and total RNA was isolated using the NucleoSpin RNA Kit (Macherey-Nagel) according to the manufacturer’ s instructions ...
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bioRxiv - Bioengineering 2023Quote: Genomic DNA was extracted from larvae or pupae of the selected lines using NucleoSpin Tissue gDNA extraction kit (Macherey-Nagel).
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bioRxiv - Bioengineering 2023Quote: We extracted RNA from live cells for bulk RNA-sequencing using the NucleoSpin totalRNA FFPE micro kit (Macherey-Nagel #740969.50) ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA was extracted from the heads of 30 male flies using the NucleoZol one phase RNA purification kit (Macherey-Nagel). Library preparation and sequencing was performed by BGI (Wuhan ...
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bioRxiv - Genomics 2022Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2023Quote: Total RNA was isolated from the cultured cells using the Nucleospin RNA Plus kit (cat. 740984, Macherey-Nagel, CA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cells from a confluent 6-well were lysed and RNA was purified using the NucleoSpin RNA Plus kit for RNA purification (740984.50, Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: cDNA constructs for mammalian tissue culture were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (no. 740410.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
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bioRxiv - Immunology 2023Quote: Total RNA from BMDMs and RAW 264.7 cells was isolated using the NucleoSpin RNA II kit (Macherey-Nagel, Catalog #740955). The cDNA of pre-mRNA was synthesized by the use of random hexamer primers (Thermo-Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... RNA from FAC-sorted lung EC was extracted using the Nucleaospin® TriPrep Kit (MACHEREY-NAGEL, 740966.50, Düren, Germany Germany). RNA quantity and quality were assessed using the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR products were purified using the NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, catalog no. 740609.50) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... We prepared a large amount of these plasmid vectors using a NucleoBond Xtra Midi Plus kit (Macherey-Nagel, Düren, Germany). Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested in RIPA with 1X HALT or the RNA lysis buffer from the Nucleospin RNA kit (Macherey-Nagel).
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were then purified using the NucleoBond PC 10000 EF Giga kit for endotoxin-free plasmid DNA from Macherey-Nagel. In parallel ...
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bioRxiv - Molecular Biology 2024Quote: ... The rest of the PCRs were purified with the NucleoSpin Gel and PCR Clean-up XS kit (#740611.50, Macherey-Nagel) for long-read amplicon sequencing using Oxford Nanopore Technology (via Plasmidsaurus).
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bioRxiv - Biochemistry 2024Quote: ... PCR products were purified using DNA gel extraction with NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL, 740609.10). Transformation was performed with purified PCR products in Stellar Competent Cells (Takara ...
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bioRxiv - Neuroscience 2024Quote: ... BAC DNA for pronuclear injection was prepared using the large construct DNA purification kit - NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al. ...
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bioRxiv - Microbiology 2024Quote: ... MDDCs were lysed and total RNA was extracted using the NucleoSpin RNA Mini kit for RNA purification (Macherey-Nagel, 740955) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Products from four PCR reactions were pooled and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel #740609). Equal amounts of products were mixed and sequenced on Illumina NextSeq 500 instruments using the NextSeq high-output kit (75 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription products were purified by NucleoSpin Gel and PCR clean-up kit equilibrated with NTC buffer (Macherey-Nagel). Dideoxy DNA sequencing samples were prepared using Thermo Sequenase DNA polymerase (Cytiva ...
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bioRxiv - Synthetic Biology 2024Quote: ... the genomic DNA of the expanded clones was extracted with a kit according to the manufacturer’s protocol (Nucleospin Tissue, Macherey-Nagel) to perform PCR genotyping ...
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bioRxiv - Plant Biology 2024Quote: ... and the resulting PCR reactions were cleaned-up using the NucleoSpin Gel and PCR Clean-up XS kit (Macherey-Nagel). All amplicons obtained from a single biological replicate were pooled in equimolar amounts and a total of 100 ng were used for library preparation following the modified Illumina Nextera Flex library preparation protocol described before (28 ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted by phenol-chloroform extraction followed by column clean-up using NucleoSpin® DNA Plant II kit (Macherey-Nagel). Clustering and DNA sequencing of wild-type ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 0.25 g (3.6 g TSS L−1) activated sludge was processed to extract intracellular DNA using a NucleoSpin® Soil kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Tissue homogenates were resuspended in RNA lysis buffer and RNA was isolated using the NucleoSpin RNA Plus RNA isolation kit (Macherey-Nagel). For some experiments ...
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bioRxiv - Genetics 2021Quote: ... DNA from three female and three male fifth instar larvae was isolated individually using the NucleoSpin DNA Insect kit (Macherey-Nagel). We observed that the primers Masc_F1 and Masc_R1 targeting EkMasc and EkMascB consistently showed off-target amplification in female samples ...
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bioRxiv - Molecular Biology 2021Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900 rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from flowers and whole chili pepper fruits at different developmental stages using a NucleoSpin(tm) RNA Plant kit (MACHEREY-NAGEL) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated from wt and Uhrf1- or Usp7-knockdown BHK cells using the nucleospin triprep kit from Macherey-Nagel. 500 ng of total RNA was reverse transcribed with a high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: ... and used for DNA extraction using the NucleoSpin Plant midi DNA extraction kit following the manufacture’s protocol (Macherey-Nagel, Düren, Germany). The genomic DNA was quality controlled using a Qubit® 3.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1.7 Gb paired-end reads for the RS_LinB were obtained from Red Sea samples preserved in CHAOS buffer (Flot 2007) and extracted using the DNA NucleoSpin kit (Macherey-Nagel) following the protocol recommended by the company ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from ~100 mg of feces with the use of a Nucleospin DNA Stool Kit (740472, Macherey-Nagel) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Systems Biology 2019Quote: ... Histone-DNA complexes were isolated from insoluble nuclear chromatin by centrifugation and DNA was extracted with a NucleoSpin PCR Clean-up kit (Macherey-Nagel). For CUT&RUN quantitative PCR ...
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bioRxiv - Genomics 2020Quote: ... for 30 min at 37 °C and gel purified with a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). The oligo-pool amplicons were assembled into the linearized Lenti-gRNA-Puro plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The amplified products were identified by gel electrophoresis and purified using Nucleospin PCR and Gel Clean Up Kit (Macherey-Nagel # 740609.5). Alternatively ...
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bioRxiv - Bioengineering 2019Quote: ... PCR products were analysed by agarose gel electrophoresis and gel purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Constructs were transformed into E ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... DNA removal was carried out via in-column treatment with the rDNAse included in the Nucleo-Spin RNA Plant Kit (Macherey-Nagel) following the instructions of the manufacturer ...
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bioRxiv - Genetics 2020Quote: ... PCR reactions were separated on agarose gels and PCR fragments isolated using a Nucleo-Spin Gel and PCR Clean-up kit (Macherey-Nagel). Sanger sequencing of PMEL and KIT ...