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Citations for Macherey-Nagel GmbH :
1701 - 1750 of 2151 citations for Pregnanediol 3 Glucuronide PDG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Total DNA was extracted from various mouse organs using the NucleoSpin Tissue kit (Macherey-Nagel Inc., Allentown, PA, USA). The DNAs were used for species-specific PCR with primers against human α-satellite and mouse plakoglobin genomic regions [35] ...
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bioRxiv - Genomics 2023Quote: A second purification step was performed using a PCR product extraction from gel agarose kit from Macherey-Nagel (MN) NucleoSpin® Gel and PCR Clean-up kit and restarting the above protocol from the step with the addition of CQW buffer then PW2 buffer.
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bioRxiv - Evolutionary Biology 2023Quote: ... They have been further purified using the Nucleospin® gDNA cleanup kit from Macherey-Nagel (Hudlow et al. 2011). The extracts were subsequently diluted in water and quantified with a Qubit® 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearised fragment was then excised and purified using the NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel). 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... DNA isolations were conducted using the NucleoSpin Plant II Mini Kit for DNA from plants (Macherey-Nagel, Düren, Germany). Preliminary quality assessments were conducted using agarose gel visualization and NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... total DNA was extracted from ∼300 mg of soil using the NucleoSpin Soil 96 kit (Macherey-Nagel, Düren, Germany). Lysis was performed using the Buffer SL with Enhancer SX and on the FastPrep 24 homogenizer (MP Biomedicals ...
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bioRxiv - Cell Biology 2024Quote: ... the first-strand cDNA was purified with the NucleoSpin gel and PCR clean-up kit (Macherey-Nagel, no. 470609) following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from approximately 100 mg of ground tissue using the NucleoSpin RNA Plant Kit (Macherey-Nagel) and treated with DNase I on the column ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from DIV4 or DIV6 primary neuronal cultures using the NucleoSpin RNA purification kit (Macherey-Nagel) and from human fibroblasts using Trizol reagent (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: BAC DNA was extracted from BAC clones (BacPac Resources, CHORI, Oakland) using the Nucleobond Xtra BAC kit (Macherey-Nagel) and subsequently labeled (Nick translation protocol ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA for quantitative reverse transcription PCR (qRT-PCR) was isolated using the NucleoSpin® RNA Kit (Macherey-Nagel), including DNA digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... Freezing and RNA isolation was done following the instructions of the NucleoSpin RNA/Protein Kit (Macherey-Nagel; Düren, Germany). 1 µg of the extracted total RNA per sample was reverse transcribed using oligo(dt)18 primers (First Strand cDNA Synthesis Kit ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR product was purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel Cat#740609.50), and resolved on a 8% polyacrylamide TBE gel (Life Technologies Cat#EC62162BOX) ...
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bioRxiv - Microbiology 2024Quote: ... All positive samples were purified with the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Each mix was then purified using the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). DNA was then eluted and quantified at a Nanodrop 2000 Spectrophotometer (ThermoScientific) ...
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bioRxiv - Molecular Biology 2024Quote: 1 µg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 µl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441) ...
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bioRxiv - Molecular Biology 2024Quote: ... and Agilent microarray analyses were performed from isolated total RNA using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250). Per reaction 200 ng RNA was amplified and Cy3-labeled using the LIRAK kit (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA containing the ACE-tRNA TTT sequences was midiprepped with the NucleoBond PC 20 miniprep kit (Macherey-Nagel). 16HBE14o-cells were diluted to 6 x 105 cells per mL with 1 mL added per well of a coated 6-well plate as previously described22 ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from 100 mg of ripe fruits using the Nucleo-spin RNA Plant mini kit (Macherey-Nagel). Library preparation and Nanopore sequencing were performed at the Ecole normale superieure genomic core facility (Paris ...
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bioRxiv - Cancer Biology 2021Quote: Cells were treated as indicated for 72 hours prior to harvesting of RNA using the Nucleospin RNA kit (Macherey-Nagel). First-strand cDNA synthesis was performed using iScript Supermix (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: RNA was purified from cerebellum samples from Cockayne patients and age- and gender-matched healthy individuals by using the Nucleospin RNA isolation kit (catalog no. 740955.250; Macherey-Nagel) with initial quantitation conducted using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... lysates prepared from infected and stimulated cells were collected and RNA extracted using a NucleoSpin RNA kit (Macherey-Nagel, 740955). The yield and purity of the extracted RNA was assessed using a spectrophotometer (DS-11 FX ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was isolated from total thymocytes and from sorted T-cell subsets from MCMV-infected and uninfected mice according to the manufacturer’s instructions using the NucleoSpin Blood QuickPure kit (MACHEREY-NAGEL), and stored at −20°C until further analysis.
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bioRxiv - Immunology 2021Quote: ... Virus was purified by centrifugation at 12,000 xg for 10 mins at 4 °C and genomic DNA extracted (NucleoSpin Tissue kit, Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Neuroscience 2021Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... the PCR product was purified with NucleoSpin Gel and PCR clean-up kit according to manufacturer’s description (Macherey-Nagel, 740609). RF cloning into pET28a was performed with eighteen 3-step cycles with annealing temperature 65°C and extension time 7 minutes ...
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bioRxiv - Genomics 2019Quote: ... PCR products were checked by agarose gel electrophoresis and purified using Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Cycle sequencing reactions were performed using the BigDye 3.1 chemistry ...
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bioRxiv - Cancer Biology 2020Quote: ... treatment with 100µg/ml RNase A (37° for 15 minutes) and 50µg/ml proteinase K and purification using the PCR cleanup kit (Macherey-Nagel).
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin® RNA II kit according to the manufacturer's protocol (Macherey-Nagel). Sixty nanograms of total RNA were reverse transcribed by using the RevertAid H Minus First Strands cDNA Synthesis Kit with oligo(dT ...
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bioRxiv - Microbiology 2019Quote: Total cellular RNA from well-differentiated hAECs was extracted with the Nucleospin™ RNA extraction kit (Macherey-Nagel, Oensingen, Switzerland) according to the manufacturer’s guidelines ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was extracted using the NucleoSpin Plant II Mini kit according to the manufacturer’s instructions (Macherey-Nagel, Hoerdt, France) with the following exception for the lysis step ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted DNA from recently collected field and greenhouse samples using NucleoSpin Plant Mini Kit II (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were cleaned up using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel, 740609.50) and sequenced using Sanger Sequencing (Department of Biochemistry ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were cleaned up using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel, 740609.50) and sent for Sanger Sequencing (Department of Biochemistry ...
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bioRxiv - Biochemistry 2020Quote: ... Genomic DNA was isolated from the pellet of trophozoite/schizont stage parasites using the Nucleospin® tissue kit (Macherey-Nagel) as instructed by the manufacturer.
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bioRxiv - Bioengineering 2021Quote: ... Unincorporated PCR primers and dNTPs were removed from the PCR products using the Nucleospin PCR clean-up kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated from sorted E14.5 fetal liver hematopoietic progenitor cells (KL; CD45+/Lineage−/cKit+/Sca1−) using the NucleoSpin RNA Plus XS kit (Macherey-Nagel). The RNA-sequencing libraries were prepared using KAPA RNA Hyper Prep Kit with RiboErase ...
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bioRxiv - Genetics 2021Quote: ... Each tissue sample was sonicated for a few seconds with the buffer LBP1 from NucleoSpin RNA Plus kits (MACHEREY-NAGEL) and divided into two tubes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA and proteins (used for the proteomic study) were extracted using the NucleoSpin® RNA/Protein extraction kit (Macherey-Nagel). RNA quantification was performed using a Nanodrop® spectrophotometer (ND-1000) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed 16 h after transfection and total RNA was extracted using the NucleoSpin RNA II kit (Macherey-Nagel).
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bioRxiv - Microbiology 2019Quote: ... PCR products were then purified using a Millipore NucleoFast 96 PCR kit following the manufacturer’s recommendations (Macherey-Nagel, Düren, Germany) and sequenced using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... and the resulting PCR products were cleaned using a NucleoSpin Gel and PCR Clean-Up kit (Macherey-Nagel, Düren, Germany). Samples were prepared for sequencing using a BigDye Terminator 3.1 kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stimulated with 15% FBS for 45 min and total RNA was extracted using Nucleospin RNA II kit according to the manufacturer’s protocol (Macherey-Nagel). Five hundred micrograms of total RNA wasused for complementary DNA synthesis using Thermo Scientific RT–PCR kit (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... After 16 hours cells were lysed and total RNA was isolated using NucleoSpin® RNA Plus Mini Kit (Macherey-Nagel) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA constructs for human Parkin mutant expression were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted by using the Macherey-Nagel Nucleospin® RNA Clean-up XS kit (Macherey-Nagel, Bethlehem, PA). cDNA was synthesized using iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... and purified directly from the PCR mix or from an agarose gel (Gel and PCR clean-up kit, Macherey-Nagel); the two fragments were fused together thanks to a primer-encoded 20-30bps overlap during a second PCR amplification round and the product cloned into the pEMG suicide vector [20] using EcoRI and BamHI ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were subjected to a final gel purification using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel).
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bioRxiv - Genomics 2022Quote: ... Midipreps for all constructs were prepared at final concentration >1mg/mL using the NucleoBond Xtra Midi Plus kit (Macherey-Nagel).
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bioRxiv - Developmental Biology 2022Quote: Total RNA was prepared from 100 mg of 11-day-old seedlings with the NucleoSpin RNA Plant Kit (Macherey-Nagel) according to the manufacturer’s instructions ...