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Citations for Macherey-Nagel GmbH :
1601 - 1650 of 2263 citations for Protein Concentration kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted from GYP treated GLUTag cells using the NucleoSpin® RNA kit (Macherey-Nagel, UK) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: mRNA from encysting and non-encysting KO strains was isolated by NucleoTrap mRNA Mini Column kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Genomic DNA was extracted using the NucleoSpin tissue mini kit for DNA from cells and tissue (Macherey-Nagel). DNA integrity was assessed by TapeStation (Agilent ...
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bioRxiv - Immunology 2022Quote: ... Supernatants collected were processed for RNA preparation using Nucleospin Viral RNA isolation kit (Macherey-Nagel GmbH & Co. KG), and infectious titer assay (plaque formation assay (PFA)) ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA (gDNA) was isolated from sorted and unsorted cells using the Nucleospin Tissue Mini Kit (Macherey-Nagel) and sgRNA sequences were retrieved by a nested PCR approach that pre-amplified sgRNAs in a first round with primers specific to the lentiGuide-Puro construct (5’-AGAGGGCCTATTTCCCATGA-3’ ...
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bioRxiv - Microbiology 2021Quote: ... the PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Purified PCR products were cloned into pGEM®-T Easy Vector (Promega ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... Cells were washed with PBS once and RNA isolated from lysed cells Nucleospin RNA kit (#740955.50, Macherey-Nagel) according to manufacturer’s instructions including rDNAse treatment ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from infected and non-infected HCT8 cells using NucleoSpin RNA Kit (Macherey-Nagel, Germany). An on-column DNase digestion with a RNase-free DNase was included in the process described by the fabricant to remove any genomic DNA contamination in RNA samples ...
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bioRxiv - Cell Biology 2022Quote: ... The dsDNA donors were then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and stored at -20°C or directly used for electroporation ...
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bioRxiv - Immunology 2022Quote: Total RNA from one hundred thousand TILs was extracted using a commercially available RNA isolation kit (Macherey-Nagel) following the manufacturer’s recommendations and ...
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bioRxiv - Cell Biology 2022Quote: ... and isolation was performed by mechanical disruption and chemically followed by NucleoSpin gDNA Clean-up Kit (Macherey-Nagel). RNA was removed with ribonucleases and genomic DNA concentration was quantified with NanoDrop ...
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bioRxiv - Plant Biology 2019Quote: ... Frozen samples were subjected to RNA extraction using the NucleoSpin RNA Plant kit (Cat# 740949, Macherey-Nagel, Germany) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2019Quote: ... The eluted DNA samples were further purified with a NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL). The DNA libraries were prepared using a SMARTer ThruPLEX Tag-seq Kit (Takara Bio) ...
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bioRxiv - Genetics 2020Quote: ... the single band was purified from a gel with a NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel) and its concentration determined ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was extracted using the NucleoSpin miRNA kit including a DNase treatment step (Macherey-Nagel, Düren, Germany) and assessed the purity and quantity of RNA with a Nanodrop 2000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: Gel-purify the excised band using the Macherey-Nagel NucleoSpin Gel & PCR Clean-up kit (Macherey-Nagel, Germany). Insert the purified array into the opened plasmid backbone using In-Fusion cloning (Takara Bio ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were purified with a NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Restriction enzymes and T4 DNA ligase were obtained from Thermo Fisher Scientific and were used according to the supplier’s specifications ...
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bioRxiv - Molecular Biology 2019Quote: ... the dried residue was dissolved in the buffer solution of NucleoSpin Tissue® kit (Macherey-Nagel Gmbh, Germany) and DNA was extracted following manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The PCR amplicon was gel-purified by using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: DNA was extracted from blood samples using the NucleoSpin Blood kit (Macherey-Nagel, GmbH & Co. KG, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified from agarose gels using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). EIF2AK2 and EIF2S1 sequences were inserted into pET-His 1a vector using the Gibson Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... Nucleic acid extraction was performed with the NucleoSpin DNA Insect Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The final plasmid library DNA was isolated from the culture using the Nucleobond Xtra Maxi kit (Macherey-Nagel).
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bioRxiv - Microbiology 2019Quote: ... The 8kb PCR fragment was gel extracted using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and eluted in P3 Buffer (Lonza ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from 2.5×105 HEK293T cells was purified using a Nucleo Spin Tissue XS kit (MACHEREY-NAGEL). Using 25 mg of human postmortem tissue ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was extracted from the samples using the NucleoSpin® DNA Food kit (Macherey-Nagel, Düren, Germany), according to the manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: Viral RNA was extracted from infected cells using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel, Hoerdt, France) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: To clone the NERCLIN cDNA we extracted total RNA from 143B cells using Mini spin kit (Macherey-Nagel). Then RNA was reverse transcribed using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher scientific) ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA was isolated using the NucleoSpin Tissue – Mini kit for DNA from cells and tissue (MACHEREY-NAGEL). Genomic DNA was sheared with a 30-G syringe ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from iPSCs and EBs using the NucleoSpin RNA Plus Kit (Macherey-Nagel, Düren, Germay), quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... beads were magnetized and 150 µl RA1 lysis buffer from NucleoSpin RNA Plus XS Kit (40990.50, Macherey-Nagel) was added to the beads ...
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bioRxiv - Microbiology 2022Quote: Total DNA was isolated from frozen cell pellets using the NucleoSpin Microbial DNA Kit (Macherey-Nagel, Düren, Germany). Methods for DNA quality control and quantification were reported previously [28] ...
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bioRxiv - Plant Biology 2023Quote: ... approximately 50 mg of ground tissue were extracted using the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany) following the user guidelines ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted following standard protocols using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany). DNA was quantified using a Qubit 3 fluorometer in combination with dsDNA BR or HS assay kits (ThermoFisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... we isolated mtDNA from postnuclear fractions with the alkaline denaturation-neutralization procedure (Nucleospin Plasmid Mini kit, Macherey-Nagel). Liver tissue was homogenized and nuclei pelleted (1000g 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Cloning was performed according to standard protocols and plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel). DNA fragments were amplified using Phusion High Fidelity DNA polymerase (NEB ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... total RNA after McIdas RNAi was isolated from U2OS cells using the Nucleospin RNA II kit (Macherey-Nagel). 1 μg RNA was converted to cDNA using M-MLV transcriptase (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA extraction was performed using a combination protocol between the NucleoSpin® Plant II kit from Macherey-Nagel and the DNeasy plant mini kit from QIAGEN ...
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bioRxiv - Plant Biology 2024Quote: ... and subjected to genomic DNA extraction using the Nucleo Mag Plant kit (Macherey-Nagel GmbH &Co. KG, Germany) in the automated nucleic acid extractor Maelstrom 4800 (TANBead Taiwan Advanced Nanotech Inc. ...
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bioRxiv - Microbiology 2023Quote: ... and the selection of inactivated cells was performed by DNA extraction using the NucleoSpin Blood kit (Macherey-Nagel) followed by PCR amplification using primers flanking the Cas9 cleavage site ...
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bioRxiv - Microbiology 2023Quote: ... and the backbone band was cut out and isolated using NucleoSpin Gel and PCR cleanup Kit (Macherey-Nagel). Backbone and insert were ligated using T4 Ligase (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... A 200 ul aliquot was used for RNA extraction using a NucleoMag Virus kit (Macherey-Nagel, Düren, Germany) and a Magnetic Particle Processor (MPP ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 g of soil were used for DNA extraction using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were purified with the rDNase set and the RNA Clean-up kit (Macherey-Nagel, Düren, Germany). RNA quality was checked with the Prokaryote Total RNA Nano Series II chip using the Agilent Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... and the resultant PCR product gel extracted using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). flaB promoter-lacI and pQE30 were Q5 PCR-amplified from pJSB252 using primers PA468 + PA471 (for pind template ...
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bioRxiv - Molecular Biology 2024Quote: ... extracted by using the NucleoSpin® Plasmid or NucleoBond® Xtra Midi Kit (Macherey-Nagel; #740588.250 and #740410.50) and controlled by Sanger Sequencing (Eurofins Genomics or Seqlab Microsynth).
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bioRxiv - Neuroscience 2024Quote: ... Maxi-preps of the plasmid construct were isolated using a Nucleobond Xtra Maxi Plus EF kit (Macherey-Nagel). The full coding frame of all pRK5-rPanx1 and pRK5-mPanx1 constructs were sequenced at the University of Calgary Sequencing Core using the primers CMV-F (5′-CGCAAATGGGCGGTAGGCGTG-3′ ...
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bioRxiv - Molecular Biology 2024Quote: ... The pUC57 mini plasmid used as carrier cDNA for transfections was gigaprepped as per kit instructions (Macherey-Nagel). The normalized ACE-tRNA library cDNA at 50 ng/μL was mixed at equal volumes with pUC57 mini carrier plasmid at 150 ng/μL to give final concentrations of 25 ng/μL and 75 ng/μL of the library and carrier plasmids respectively.
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bioRxiv - Cancer Biology 2024Quote: ... and genomic DNA was extracted using the Nucleospin Blood L Midi genomic DNA extraction kit (Macherey-Nagel, 740954.20). Genomic DNA was PCR amplified by the Genetic Perturbation Platform at the Broad Institute using the following primers:
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bioRxiv - Microbiology 2024Quote: ... DNA fragments and PCR products were purified using the Nucleospin Gel and PCR clean up kit (Macherey-Nagel).