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Citations for Macherey-Nagel GmbH :
1601 - 1650 of 2323 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Extracted RNA was subsequently enriched for polyadenylated mRNA transcripts using the NucleoTrap mRNA enrichment kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: RNA from viral supernatants was isolated using Nucleospin Viral RNA isolation kit (Macherey-Nagel GmbH & Co. KG). qRT-PCR was carried out using nCOV-19 RT-PCR detection kit from Q-line Molecular to quantify SARS-CoV-2 RNA following manufacturer’s protocol on Roche LightCycler 480.
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bioRxiv - Synthetic Biology 2022Quote: ... We then collected spheroplasts by centrifugation and isolated RNA using the NucleoSpin RNA Plus kit (Macherey-Nagel). We evaluated RNA quality and integrity by Qubit fluorometry using an RNA BR Assay Kit (Thermo) ...
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bioRxiv - Synthetic Biology 2022Quote: ... gDNA was extracted from 10 individuals from pools bgcn-Cas9D using the NucleoSpin Tissue kit (Macherey-Nagel). DNA was digested with the restriction enzymes BamHI ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA isolation was performed according to the manufacture protocol with NucleoSpin RNA Plus kit (Macherey-Nagel). RNA was retrotranscribed with Reverse Transcriptase Superscript IV (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... and DNA was purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel; #740609.50) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmids were purified with the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Genetics 2024Quote: ... while the ZB5d/antibiotics mixture was further processed with the NucleoMag VET Kit (Macherey-Nagel, Düren, Germany) in a final elution volume of 100µL VEL-buffer using a KingFisher®Flex automat (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using 100 µL of homogenate with the Genomic DNA tissue kit (Macherey-Nagel, Germany). Detection and quantification of R ...
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bioRxiv - Microbiology 2024Quote: ... The PCR amplicons were gel purified with NucleoSpin gel and PCR clean-up kits (Macherey-Nagel, Germany) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel, 740609.50). DNA amount was measured using a Nanodrop ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was extracted from wild-type worms using the Nucleospin RNA® kit (Macherey-Nagel – 740955) or Trizol Reagent™ (Ambion® ...
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bioRxiv - Immunology 2023Quote: ... RNA from tumours was extracted and purified from tumours using the NucleoSpin RNA extraction kit (Macherey-Nagel). Library preparation and sequencing were performed by Genewiz (Leipzig ...
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bioRxiv - Plant Biology 2023Quote: RNA samples were extracted using the Nucleospin plant RNA extraction kit (Macherey-Nagel GmbH & Co. KG, Germany). cDNAs were synthesized using PrimeScript RT Mastermix (Takara Bio Inc. ...
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bioRxiv - Cell Biology 2023Quote: Large RNA above 200 nucleotides were isolated from macrophage samples using the NucleoSpin miRNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Spheroplasts were collected by centrifugation and RNA was isolated using the NucleoSpin RNA Plus kit (Macherey-Nagel). RNA quality and integrity was determined by Qubit fluorometry using an RNA BR Assay Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... the PCR product was purified using a NucleoSpin Gel and PCR Clean-up purification kit (Macherey-Nagel) and subjected to Sanger sequencing at Eurofins Genomics GmbH (Ebersberg ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from the resulting pellets using the NucleoMag DNA Kit (Macherey-Nagel, Allentown, PA, USA) with the aid of the AutoPure96 robot for the purification step ...
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bioRxiv - Microbiology 2023Quote: ... and total RNAs were extracted from the samples using a NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). cDNAs were synthesized from the extracted RNAs using a PrimeScript Double Strand cDNA Synthesis Kit (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany), quantified using the Quant-iT PicoGreen dsDNA kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany); their concentration was brought to 30 ng/μl ...
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bioRxiv - Cell Biology 2023Quote: ... before RNA was purified using the NucleoSpin miRNA Mini kit for miRNA and RNA purification (Macherey-Nagel) without size fractionation ...
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bioRxiv - Plant Biology 2024Quote: ... the PCR products were purified using a NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). Sequencing reactions were made up of 20-25/ng of the purified PCR product ...
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bioRxiv - Microbiology 2024Quote: ... PCR products for assembly were purified using the NucleoSpin Gel and PCR Clean- up Kit (Macherey-Nagel), and plasmids from positive clones were extracted using the NucleoSpin Plasmid Kit (Macherey-Nagel) ...
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bioRxiv - Genomics 2022Quote: ... The PCR products were gel extracted using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... and cleaned using the NucleoSpin© Gel and PCR clean-up kit (Macherey-Nagel, cat. no. 740609) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from iPSCs and EBs using the NucleoSpin Tissue Kit (Macherey-Nagel, Düren, Germany) with the manufacturer’s instructions and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... the PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel #740609). Purified DNA was cloned into pAV0714 (Vještica et al. ...
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bioRxiv - Genetics 2022Quote: ... Gametophyte DNA was extracted using the Macherey-Nagel NucleoSpin Plant II Maxi Kit (Macherey-Nagel, Duren, Germany) with a modified protocol ...
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bioRxiv - Physiology 2022Quote: The recombined Ptger3-tTA-BGH BAC was purified with a Nucleobond BAC100 kit (Macherey-Nagel, Düren, Germany) and the DNA was digested for 1 h with I-SceI (New England BioLabs ...
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA from thirty-three clones and WT tachyzoites extracted using NucleoSpin DNA RapidLyse kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Viral RNA from the supernatant was extracted using the NucleoSpin RNA Virus Isolation Kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were purified using a PCR clean-up kit as per manufacturer’s instructions (Macherey-Nagel, Germany) followed by agarose (1 w/v % ...
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bioRxiv - Bioengineering 2022Quote: ... The cssDNA released from the phage were then extracted with NucleoBond Xtra Midi EF kit (Macherey-Nagel) following the manufacturer’ s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and excised from a 0.8% agarose gel for purification using the NucleoSpin Gel Cleanup Kit (Macherey-Nagel). The insert pool and digested backbone were then ligated overnight at 16°C using T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... Gel bands were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, N. 740609.50) and sequenced by Sanger sequencing.
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted right after sorting using the NucleoSpin® RNA kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Plant Biology 2023Quote: ... fragments of interest were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel), and sequenced with Eurofins SupremeRun reactions.
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bioRxiv - Cell Biology 2023Quote: Total RNA from heart tissue was extracted with the NucleoSpin RNA Mini Kit with DNase (Macherey-Nagel). cDNA was synthesized using oligo dT with the Flex Script cDNA Synthesis kit (Quanta bio) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA of progeny virions was extracted from supernatants using the NucleoSpin RNA virus kit (Macherey-Nagel, #740956) according to the manufacturer’s instructions and stored at −80 °C until PCR-based analysis.
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bioRxiv - Developmental Biology 2024Quote: ... HH25 (E4) or HH28 (E5) chick embryos using the NucleoSpin RNA XS kit (740902-10, Macherey-Nagel). One µg of RNA was used for the reverse-transcription using RevertAid H Minus First Strand cDNA Synthesis Kit (K1632 ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from the primary CEC lines using a NucleoSpin RNA XS kit (Macherey-Nagel), in accordance with the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: RNA purification and reverse transcription were performed using the Nucleospin RNA kit from Macherey-Nagel (Düren, Germany) and the High capacity cDNA reverse transcription kit from Applied Biosystems (Woolston ...
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bioRxiv - Microbiology 2024Quote: ... The last purification step was performed with the RNA Nucleospin kit according to manufacturer’s instructions (Macherey-Nagel). The integrity of the RNAs was verified using the Agilent 2100 bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We extracted total genomic DNA from each whole individual sample using NucleoMag® Tissue Kit (Macherey-Nagel) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted using a modified protocol of the Nucleospin 96 RNA kit (Macherey-Nagel, Duren, Germany). Cells were lysed in a 96 deep-well plate by adding 450μl of lysis buffer containing 1M sorbitol (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... PCR-amplified DNA was cleaned with NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmid DNA was purified using QIAprep Spin Miniprep Kit (QIAGEN ...
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Transcription factors SlMYB41, SlMYB92 and SlWRKY71 regulate gene expression in the tomato exodermisbioRxiv - Plant Biology 2024Quote: ... The PCR products were gel-purified using the kit NucleoSpin Gel and PCR Clean-up (MACHEREY-NAGEL). We cloned all the promoters ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA products were clean up using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel, 740609.250). Primers targeting CLCN4 were designed using the NCBI Primer-BLAST tool [35] ...
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bioRxiv - Microbiology 2024Quote: DNA plasmids were amplified from DH5α cultures and purified using endotoxin-free DNA extraction kits (Macherey-Nagel) as previously described (28) ...