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Citations for Macherey-Nagel GmbH :
1551 - 1600 of 2124 citations for Mouse Protein FAM40A FAM40A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Cloning was performed according to standard protocols and plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel). DNA fragments were amplified using Phusion High Fidelity DNA polymerase (NEB ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... total RNA after McIdas RNAi was isolated from U2OS cells using the Nucleospin RNA II kit (Macherey-Nagel). 1 μg RNA was converted to cDNA using M-MLV transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used in cloning reactions were purified using the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA were extracted from GBM control and EMP3 KO cells using the Nucleospin RNA kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Its DNA was extracted from mycelium and conidia with the NucleoBond High Molecular Weight DNA kit (Macherey-Nagel), with the mechanical disruption of about 30 mg of lyophilized mycelium with beads for 5 min at 30 Hz ...
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bioRxiv - Neuroscience 2023Quote: ... genomic DNA was extracted from a single F1 mosquito using the NucleoSpin Tissue kit (Macherey-Nagel Inc., 740952.50). A diagnostic PCR was then performed to fully amplify the Act5C-ECFP marker using the primer ...
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bioRxiv - Molecular Biology 2023Quote: DNA samples was extracted from different salmon tissues using the NucleoSpin® Tissue kit (Macherey-Nagel, no. 740741) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... The gDNA was isolated from the transgenic parasite lines using the NucleoSpin Blood Kit (Macherey-Nagel; Dueren, DE) following the manufacturer’s protocol and used as template to verify the successful vector integration by diagnostic PCR (for primer sequence ...
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bioRxiv - Genomics 2023Quote: ... The DNA donors were then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and stored at -20°C or directly used for electroporation ...
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bioRxiv - Cancer Biology 2022Quote: ... The library stock was prepared by scraping plates followed by purification using the Midi Prep kit (Macherey-Nagel). Library sequencing was performed on a MiSeq run to ensure sgRNA uniformity ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were evaluated by electrophoresis and then further processed with the PCR clean-up Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from tissues or purified cells using the the NucleoSpin® RNA Plus Kit (Macherey-Nagel). Complementary DNAs were synthesized using the SuperScript™ III First-Strand Synthesis System Kit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: RNA extraction of in vitro cultured Th17 cells was performed with the NucleoSpin RNA XS Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and the reverse transcription products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). The reverse transcription products were eluted with 30 μL of HiDi formamide (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were purified via gel extraction using a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). Illumina sequencing adapters were added with Phusion High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Microbiology 2023Quote: mRNA of total colonic tissue or small intestinal organoids was isolated using the NucleoSpin RNAII kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... All probes were gel purified using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel, cat# 740609). The pixel intensities of the bands corresponding to linear ...
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bioRxiv - Genetics 2023Quote: ... PCR reactions were purified using a PCR/Gel purification kit (NucleoSpin Gel and PCR Clean□up, Macherey-Nagel).
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bioRxiv - Genetics 2023Quote: ... the fragments were purified using a PCR/Gel purification kit (NucleoSpin Gel and PCR Clean□up, Macherey-Nagel) and sequenced using the smt0-seq-Rv oligonucleotide ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from fresh bacterial cultures using the NucleoSpin® 96 Tissue Core Kit (Macherey-Nagel, Germany). All DNA samples were sequenced at the Max Planck-Genome-Centre Cologne using the Illumina paired-end technology with read lengths of 150 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified by a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel., Düren, Germany). COI gene fragments were amplified from some samples according to the USDA recommended protocol (https://www.fsis.usda.gov/sites/default/files/media_file/2020-11/CLG-FPCR.pdf ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were subsequently purified using the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Systems Biology 2023Quote: ... and 230 μg of DNA was extracted using the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany). Efficiency of the re-circularization was checked by Sanger sequencing of eight clones ...
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bioRxiv - Immunology 2023Quote: ... purified again via agarose gel separation and using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany), before being pooled and submitted to GATC Biotech (Germany) ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments and PCR products were purified using the Nucleospin Gel and PCR clean up kit (Macherey-Nagel).
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bioRxiv - Microbiology 2024Quote: ... RNA was column purified using NTC buffer and the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: Serum samples from all experimental groups were extracted using the NucleoSpin RNA isolation kit (Macherey-Nagel, Allentown, PA). Following viral RNA extraction ...
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bioRxiv - Genomics 2024Quote: High-molecular-weight DNA was extracted with the NucleoBond® HMW DNA kit (Macherey-Nagel Gmbh & Co, Germany) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and DNA bands of interest were isolated using the NucleoSpin® Gel & PCR Cleanup Kit from Macherey-Nagel. The extracted DNA underwent Sanger sequencing at Eurofins Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... PCR samples were then purified using following the manufacturer’s instructions (NucleoSpin® PCR and gel kit, Macherey-Nagel). Purified amplicons were verified by electrophoresis on a 1% agarose gel ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA extraction was performed using a combination protocol between the NucleoSpin® Plant II kit from Macherey-Nagel and the DNeasy plant mini kit from QIAGEN ...
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bioRxiv - Plant Biology 2024Quote: ... and subjected to genomic DNA extraction using the Nucleo Mag Plant kit (Macherey-Nagel GmbH &Co. KG, Germany) in the automated nucleic acid extractor Maelstrom 4800 (TANBead Taiwan Advanced Nanotech Inc. ...
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bioRxiv - Microbiology 2023Quote: ... and the selection of inactivated cells was performed by DNA extraction using the NucleoSpin Blood kit (Macherey-Nagel) followed by PCR amplification using primers flanking the Cas9 cleavage site ...
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bioRxiv - Microbiology 2023Quote: ... and the backbone band was cut out and isolated using NucleoSpin Gel and PCR cleanup Kit (Macherey-Nagel). Backbone and insert were ligated using T4 Ligase (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... A 200 ul aliquot was used for RNA extraction using a NucleoMag Virus kit (Macherey-Nagel, Düren, Germany) and a Magnetic Particle Processor (MPP ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 g of soil were used for DNA extraction using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were purified with the rDNase set and the RNA Clean-up kit (Macherey-Nagel, Düren, Germany). RNA quality was checked with the Prokaryote Total RNA Nano Series II chip using the Agilent Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... and the resultant PCR product gel extracted using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). flaB promoter-lacI and pQE30 were Q5 PCR-amplified from pJSB252 using primers PA468 + PA471 (for pind template ...
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bioRxiv - Molecular Biology 2024Quote: ... extracted by using the NucleoSpin® Plasmid or NucleoBond® Xtra Midi Kit (Macherey-Nagel; #740588.250 and #740410.50) and controlled by Sanger Sequencing (Eurofins Genomics or Seqlab Microsynth).
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bioRxiv - Neuroscience 2024Quote: ... Maxi-preps of the plasmid construct were isolated using a Nucleobond Xtra Maxi Plus EF kit (Macherey-Nagel). The full coding frame of all pRK5-rPanx1 and pRK5-mPanx1 constructs were sequenced at the University of Calgary Sequencing Core using the primers CMV-F (5′-CGCAAATGGGCGGTAGGCGTG-3′ ...
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bioRxiv - Molecular Biology 2024Quote: ... The pUC57 mini plasmid used as carrier cDNA for transfections was gigaprepped as per kit instructions (Macherey-Nagel). The normalized ACE-tRNA library cDNA at 50 ng/μL was mixed at equal volumes with pUC57 mini carrier plasmid at 150 ng/μL to give final concentrations of 25 ng/μL and 75 ng/μL of the library and carrier plasmids respectively.
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bioRxiv - Cancer Biology 2024Quote: ... and genomic DNA was extracted using the Nucleospin Blood L Midi genomic DNA extraction kit (Macherey-Nagel, 740954.20). Genomic DNA was PCR amplified by the Genetic Perturbation Platform at the Broad Institute using the following primers:
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bioRxiv - Biochemistry 2024Quote: Fosmid DNA of the hit clones was extracted with the NucleoBond Xtra Midi kit from Macherey-Nagel (France) following supplier recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... SNU-499 and Hep3B cells was extracted using the NucleoSpin RNA kit (Macherey-Nagel, AH Diagnostics, Solna, Sweden) according to the protocol of the manufacturers ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated using the NucleoSpin® RNA Plus kit following the manufacturer’s protocol (Macherey-Nagel, Düren, Germany). The integrity and concentration of RNA samples was assessed using the TapeStation 2200 (Agilent ...
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bioRxiv - Systems Biology 2020Quote: The viral RNA from supernatant of infected cells was isolated using the NucleoSpin RNA virus isolation kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... were grown overnight at 37 °C and plasmid was subsequently purified using the NucleoBond Xtra Midi kit (Macherey-Nagel). Plasmid DNA was solubilized in 150 µl of TE buffer and quantified using NanoDrop ND-1000 spectrophotometer (Celbio) ...
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bioRxiv - Molecular Biology 2021Quote: ... Fragments of 300–2000 bp were excised from the gel and isolated with a gel-extraction kit (Macherey-Nagel, NucleoSpin Gel and PCR Clean-up ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplicons were purified using the NucleoSpin® Gel and PCR clean up kits from Macherey-Nagel (Bethlehem, PA), after which quantification and qualification of purified band(s ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA for short-read sequencing was extracted using NucleoSpin® Tissue kit (Macherey-Nagel, GmbH & Co. KG, Germany), library was prepared by Nextera® XT Library Preparation kit (Illumina ...