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Citations for Macherey-Nagel GmbH :
1551 - 1600 of 2144 citations for Mouse Eukaryotic translation initiation factor 4E binding protein 3 EIF4EBP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: ... Cells were washed with PBS once and RNA isolated from lysed cells Nucleospin RNA kit (#740955.50, Macherey-Nagel) according to manufacturer’s instructions including rDNAse treatment ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from infected and non-infected HCT8 cells using NucleoSpin RNA Kit (Macherey-Nagel, Germany). An on-column DNase digestion with a RNase-free DNase was included in the process described by the fabricant to remove any genomic DNA contamination in RNA samples ...
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bioRxiv - Cell Biology 2022Quote: ... The dsDNA donors were then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and stored at -20°C or directly used for electroporation ...
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bioRxiv - Immunology 2022Quote: Total RNA from one hundred thousand TILs was extracted using a commercially available RNA isolation kit (Macherey-Nagel) following the manufacturer’s recommendations and ...
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bioRxiv - Cell Biology 2022Quote: ... and isolation was performed by mechanical disruption and chemically followed by NucleoSpin gDNA Clean-up Kit (Macherey-Nagel). RNA was removed with ribonucleases and genomic DNA concentration was quantified with NanoDrop ...
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bioRxiv - Plant Biology 2019Quote: ... Frozen samples were subjected to RNA extraction using the NucleoSpin RNA Plant kit (Cat# 740949, Macherey-Nagel, Germany) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2019Quote: ... The eluted DNA samples were further purified with a NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL). The DNA libraries were prepared using a SMARTer ThruPLEX Tag-seq Kit (Takara Bio) ...
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bioRxiv - Genetics 2020Quote: ... the single band was purified from a gel with a NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel) and its concentration determined ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was extracted using the NucleoSpin miRNA kit including a DNase treatment step (Macherey-Nagel, Düren, Germany) and assessed the purity and quantity of RNA with a Nanodrop 2000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: Gel-purify the excised band using the Macherey-Nagel NucleoSpin Gel & PCR Clean-up kit (Macherey-Nagel, Germany). Insert the purified array into the opened plasmid backbone using In-Fusion cloning (Takara Bio ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were purified with a NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Restriction enzymes and T4 DNA ligase were obtained from Thermo Fisher Scientific and were used according to the supplier’s specifications ...
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bioRxiv - Molecular Biology 2019Quote: ... the dried residue was dissolved in the buffer solution of NucleoSpin Tissue® kit (Macherey-Nagel Gmbh, Germany) and DNA was extracted following manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The PCR amplicon was gel-purified by using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: DNA was extracted from blood samples using the NucleoSpin Blood kit (Macherey-Nagel, GmbH & Co. KG, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified from agarose gels using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). EIF2AK2 and EIF2S1 sequences were inserted into pET-His 1a vector using the Gibson Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... Nucleic acid extraction was performed with the NucleoSpin DNA Insect Kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The final plasmid library DNA was isolated from the culture using the Nucleobond Xtra Maxi kit (Macherey-Nagel).
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bioRxiv - Microbiology 2019Quote: ... The 8kb PCR fragment was gel extracted using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and eluted in P3 Buffer (Lonza ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA from 2.5×105 HEK293T cells was purified using a Nucleo Spin Tissue XS kit (MACHEREY-NAGEL). Using 25 mg of human postmortem tissue ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was extracted from the samples using the NucleoSpin® DNA Food kit (Macherey-Nagel, Düren, Germany), according to the manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: Viral RNA was extracted from infected cells using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel, Hoerdt, France) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: To clone the NERCLIN cDNA we extracted total RNA from 143B cells using Mini spin kit (Macherey-Nagel). Then RNA was reverse transcribed using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher scientific) ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA was isolated using the NucleoSpin Tissue – Mini kit for DNA from cells and tissue (MACHEREY-NAGEL). Genomic DNA was sheared with a 30-G syringe ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from iPSCs and EBs using the NucleoSpin RNA Plus Kit (Macherey-Nagel, Düren, Germay), quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... beads were magnetized and 150 µl RA1 lysis buffer from NucleoSpin RNA Plus XS Kit (40990.50, Macherey-Nagel) was added to the beads ...
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bioRxiv - Microbiology 2022Quote: Total DNA was isolated from frozen cell pellets using the NucleoSpin Microbial DNA Kit (Macherey-Nagel, Düren, Germany). Methods for DNA quality control and quantification were reported previously [28] ...
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bioRxiv - Plant Biology 2023Quote: ... approximately 50 mg of ground tissue were extracted using the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany) following the user guidelines ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted following standard protocols using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany). DNA was quantified using a Qubit 3 fluorometer in combination with dsDNA BR or HS assay kits (ThermoFisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... we isolated mtDNA from postnuclear fractions with the alkaline denaturation-neutralization procedure (Nucleospin Plasmid Mini kit, Macherey-Nagel). Liver tissue was homogenized and nuclei pelleted (1000g 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Cloning was performed according to standard protocols and plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel). DNA fragments were amplified using Phusion High Fidelity DNA polymerase (NEB ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... total RNA after McIdas RNAi was isolated from U2OS cells using the Nucleospin RNA II kit (Macherey-Nagel). 1 μg RNA was converted to cDNA using M-MLV transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used in cloning reactions were purified using the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA were extracted from GBM control and EMP3 KO cells using the Nucleospin RNA kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Its DNA was extracted from mycelium and conidia with the NucleoBond High Molecular Weight DNA kit (Macherey-Nagel), with the mechanical disruption of about 30 mg of lyophilized mycelium with beads for 5 min at 30 Hz ...
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bioRxiv - Neuroscience 2023Quote: ... genomic DNA was extracted from a single F1 mosquito using the NucleoSpin Tissue kit (Macherey-Nagel Inc., 740952.50). A diagnostic PCR was then performed to fully amplify the Act5C-ECFP marker using the primer ...
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bioRxiv - Molecular Biology 2023Quote: DNA samples was extracted from different salmon tissues using the NucleoSpin® Tissue kit (Macherey-Nagel, no. 740741) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... The gDNA was isolated from the transgenic parasite lines using the NucleoSpin Blood Kit (Macherey-Nagel; Dueren, DE) following the manufacturer’s protocol and used as template to verify the successful vector integration by diagnostic PCR (for primer sequence ...
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bioRxiv - Genomics 2023Quote: ... The DNA donors were then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and stored at -20°C or directly used for electroporation ...
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bioRxiv - Cancer Biology 2022Quote: ... The library stock was prepared by scraping plates followed by purification using the Midi Prep kit (Macherey-Nagel). Library sequencing was performed on a MiSeq run to ensure sgRNA uniformity ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were evaluated by electrophoresis and then further processed with the PCR clean-up Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from tissues or purified cells using the the NucleoSpin® RNA Plus Kit (Macherey-Nagel). Complementary DNAs were synthesized using the SuperScript™ III First-Strand Synthesis System Kit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: RNA extraction of in vitro cultured Th17 cells was performed with the NucleoSpin RNA XS Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and the reverse transcription products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). The reverse transcription products were eluted with 30 μL of HiDi formamide (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were purified via gel extraction using a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). Illumina sequencing adapters were added with Phusion High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Microbiology 2023Quote: mRNA of total colonic tissue or small intestinal organoids was isolated using the NucleoSpin RNAII kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... All probes were gel purified using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel, cat# 740609). The pixel intensities of the bands corresponding to linear ...
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bioRxiv - Genetics 2023Quote: ... PCR reactions were purified using a PCR/Gel purification kit (NucleoSpin Gel and PCR Clean□up, Macherey-Nagel).
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bioRxiv - Genetics 2023Quote: ... the fragments were purified using a PCR/Gel purification kit (NucleoSpin Gel and PCR Clean□up, Macherey-Nagel) and sequenced using the smt0-seq-Rv oligonucleotide ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from fresh bacterial cultures using the NucleoSpin® 96 Tissue Core Kit (Macherey-Nagel, Germany). All DNA samples were sequenced at the Max Planck-Genome-Centre Cologne using the Illumina paired-end technology with read lengths of 150 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified by a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel., Düren, Germany). COI gene fragments were amplified from some samples according to the USDA recommended protocol (https://www.fsis.usda.gov/sites/default/files/media_file/2020-11/CLG-FPCR.pdf ...