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Citations for Macherey-Nagel GmbH :
1451 - 1500 of 2266 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... The PCR amplicons were purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany) and sequenced by Eurofin Genomics K.K ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using Nucleospin® 96 Tissue Kit according to the manufacturer’s protocol (Macherey-Nagel, Germany). Individual double-digest RAD-seq libraries were constructed with PstI and MseI according to the protocol detailed in Brelsford ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted from 24h treated samples using the NucleoSpin® RNA kit (Macherey-Nagel, UK) and cDNA was synthesised using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The viral RNA (vRNA) in the culture supernatant (SN) was extracted using the NucleoSpin kit (Macherey-Nagel), according to the manufacturer’s instructions and quantified by real-time quantitative polymerase chain reaction (RT-qPCR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we extracted DNA from liver tissue of a single mouse using a Nucleospin Tissue Kit (Macherey-Nagel) and quantified the DNA with a QuantiFluor dsDNA System (Promega ...
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bioRxiv - Plant Biology 2021Quote: ... ∼500 mg of rhizosphere soil was used for the NucleoSpin Soil gDNA Extraction Kit (Macherey-Nagel, Germany), and ∼130 mg of roots for the DNeasy Plant DNA Extraction Kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA was extracted with a NucleoSpin RNA isolation kit (#740955.50, Macherey-Nagel GmbH & Co. Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Protino® Ni-NTA Agarose and NucleoBond® Midi Plasmid DNA Purification Kit were from Macherey-Nagel, Germany ...
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bioRxiv - Evolutionary Biology 2021Quote: ... total DNA was extracted using either the NucleoSpin® Plant II Midi Kit (Macherey-Nagel, Düren, Germany) or a modified CTAB protocol (Rogers and Bendich 1985 ...
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bioRxiv - Microbiology 2021Quote: ... Total RNAs from harvested cells were extracted using the NucleoSpin RNA extraction kit (Cat. # 740955.250, MACHEREY-NAGEL), and 0.4-1 μg RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Cat ...
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bioRxiv - Genomics 2020Quote: ... PCR products were gel-purified using a NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel 740609) followed by Sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel, Oensingen, Switzerland) from which the nucleotide sequence was determined using Sanger sequencing (Microsynth ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were then purified using NucleoSpin®Gel and PCR Clean up kit (Macherey-Nagel™). Final elution product was divided in two for sequencing by Sanger (after cloning ...
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bioRxiv - Immunology 2021Quote: ... RNA from cultured ILC2s was extracted using a NucleoSpin RNA Kit according to manufacturer’s instructions (MACHEREY-NAGEL). The concentration and purity of extracted RNA was measured using a Spectrophotometer NanoDrop 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA was extracted as pooled libraries using NucleoBond Xtra Maxi EF kit (Macherey-Nagel, Duere, Germany) and stored at −20 ℃.
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: RNA was isolated from MEFs or NIH3T3 cells using the Nucleospin RNA plus kit (Macherey-Nagel, 740984.5). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated from whole blood samples using the NucleoSpin 96 RNA tissue kit protocol (Macherey-Nagel) with some modifications as previously described15.
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from cell culture fluids using the NucleoSpin RNA Virus Kit (Macherey-Nagel, Germany). Total RNA from cells was isolated using TRIreagent (Sigma ...
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bioRxiv - Immunology 2020Quote: Viral RNA was extracted from the infected cells using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel) and Covid-19 virus detection was performed using One-Step qRT-PCR SuperScript™ III Platinum™ (Life Technologies ...
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bioRxiv - Immunology 2021Quote: Total cellular RNA from IFN-treated hAEC cultures was extracted with the NucleoMag RNA kit (Macherey-Nagel) according to the manufacturer’s guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
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bioRxiv - Genomics 2020Quote: ... We extracted genomic DNA using NucleoSpin Microbial DNA extraction kit according to the manufacturer’s instructions (Macherey-Nagel). Genomic DNA for strain Y55 was extracted independently using the QIAGEN Blood & Culture DNA Midi Kit ...
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bioRxiv - Genetics 2021Quote: ... coli (High Efficiency) cells and subsequent plasmid isolation was performed using the NucleoSpin Plasmid kit (Macherey-Nagel).
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bioRxiv - Microbiology 2023Quote: ... The pBAC infectious clone plasmid was extracted and purified using NucleoBond Xtra Maxi prep kit (Macherey-Nagel). The plasmid was then sequenced using Primordium Labs “Large” whole plasmid sequencing service ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used for cloning reactions were purified by using the Nucleospin gel extraction kit (Macherey-Nagel) according to the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2023Quote: ... DIA and HR muscles using the KingFisher Flex device and the NucleoMag Pathogen kit (744210.4, Macherey-Nagel). Briefly ...
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bioRxiv - Genomics 2023Quote: ... was purified from a 1% agarose gel using a Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The genomic DNA used for WGBS was prepared using a NucleoSpin Tissue Kit (Macherey-Nagel, Dueren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The digested vector was purified using the NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel™, 740609) and then ligated with annealed gRNA oligos ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA of all samples was then isolated using columns of a NucleoSpin RNA kit (Macherey-Nagel). The concentration and quality of the derived RNA were monitored by using NanoDrop 2000 system (NanoDrop Technology) ...
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bioRxiv - Genomics 2023Quote: Small RNA was isolated from frozen and ground flower samples using a NucleoSpin miRNA kit (Macherey-Nagel) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was isolated from rosette leaves using the NucleoBond HMW DNA Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... the vector backbone was gel-purified using the NucleoSpin Gel and PCR Clean‐up kit (Macherey-Nagel). Oligonucleotides pegRNA3-1 and pegRNA3-2 (Appendix Tables S1 and S2 ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR fragments were further subjected to PCR clean-up using PCR clean-up kit (Macherey-Nagel™). Electrocompetent cells were produced according to the Invitrogen Pichia Expression Kit (Catalogue no ...
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bioRxiv - Microbiology 2022Quote: ... ~500 mg of rhizosphere soil was used for the NucleoSpin Soil gDNA Extraction Kit (Macherey-Nagel, Germany), and ~130 mg of roots for the DNeasy Plant DNA Extraction Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... was digested with BsmBI and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Forward and reverse oligos containing the guide sequence of interest were phosphorylated and annealed and ligated into the parental plasmid with T4 PNK and T4 DNA ligase ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted RNA was subsequently enriched for polyadenylated mRNA transcripts using the NucleoTrap mRNA enrichment kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: RNA from viral supernatants was isolated using Nucleospin Viral RNA isolation kit (Macherey-Nagel GmbH & Co. KG). qRT-PCR was carried out using nCOV-19 RT-PCR detection kit from Q-line Molecular to quantify SARS-CoV-2 RNA following manufacturer’s protocol on Roche LightCycler 480.
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bioRxiv - Synthetic Biology 2022Quote: ... We then collected spheroplasts by centrifugation and isolated RNA using the NucleoSpin RNA Plus kit (Macherey-Nagel). We evaluated RNA quality and integrity by Qubit fluorometry using an RNA BR Assay Kit (Thermo) ...
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bioRxiv - Synthetic Biology 2022Quote: ... gDNA was extracted from 10 individuals from pools bgcn-Cas9D using the NucleoSpin Tissue kit (Macherey-Nagel). DNA was digested with the restriction enzymes BamHI ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA isolation was performed according to the manufacture protocol with NucleoSpin RNA Plus kit (Macherey-Nagel). RNA was retrotranscribed with Reverse Transcriptase Superscript IV (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... and DNA was purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel; #740609.50) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmids were purified with the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Genetics 2024Quote: ... while the ZB5d/antibiotics mixture was further processed with the NucleoMag VET Kit (Macherey-Nagel, Düren, Germany) in a final elution volume of 100µL VEL-buffer using a KingFisher®Flex automat (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using 100 µL of homogenate with the Genomic DNA tissue kit (Macherey-Nagel, Germany). Detection and quantification of R ...
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bioRxiv - Microbiology 2024Quote: ... The PCR amplicons were gel purified with NucleoSpin gel and PCR clean-up kits (Macherey-Nagel, Germany) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel, 740609.50). DNA amount was measured using a Nanodrop ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was extracted from wild-type worms using the Nucleospin RNA® kit (Macherey-Nagel – 740955) or Trizol Reagent™ (Ambion® ...
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bioRxiv - Immunology 2023Quote: ... RNA from tumours was extracted and purified from tumours using the NucleoSpin RNA extraction kit (Macherey-Nagel). Library preparation and sequencing were performed by Genewiz (Leipzig ...
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bioRxiv - Plant Biology 2023Quote: RNA samples were extracted using the Nucleospin plant RNA extraction kit (Macherey-Nagel GmbH & Co. KG, Germany). cDNAs were synthesized using PrimeScript RT Mastermix (Takara Bio Inc. ...
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bioRxiv - Cell Biology 2023Quote: Large RNA above 200 nucleotides were isolated from macrophage samples using the NucleoSpin miRNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...