Labshake search
Citations for Macherey-Nagel GmbH :
101 - 150 of 184 citations for Chocolate Agar with Bacitracin for Haemophilus isolation 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... RNA from dry pellets of Vγ9Vδ2 T lymphocytes was extracted and purified using the NucleoSpin RNA plus isolation kit from Macherey-Nagel. DGE-sequencing was performed by the BIRD core facility and data were analyzed using R programming languages and tools.
-
bioRxiv - Molecular Biology 2024Quote: ... Freezing and RNA isolation was done following the instructions of the NucleoSpin RNA/Protein Kit (Macherey-Nagel; Düren, Germany). 1 µg of the extracted total RNA per sample was reverse transcribed using oligo(dt)18 primers (First Strand cDNA Synthesis Kit ...
-
The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: RNA isolation for multiple tissues was achieved by using the NucleoZOL one phase RNA purification kit (Macherey-Nagel #740404.200) following manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... DNA isolations were conducted using the NucleoSpin Plant II Mini Kit for DNA from plants (Macherey-Nagel, Düren, Germany). Preliminary quality assessments were conducted using agarose gel visualization and NanoDrop (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2024Quote: RNA from 10 mL yeast culture was isolated using a mini kit for RNA isolation (Macherey-NAGEL, REF 740933.50). Next ...
-
bioRxiv - Cell Biology 2020Quote: RNA was purified from cerebellum samples from Cockayne patients and age- and gender-matched healthy individuals by using the Nucleospin RNA isolation kit (catalog no. 740955.250; Macherey-Nagel) with initial quantitation conducted using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA from ΔbolA and control cell line was isolated independently six times using NucleoSpinTM RNA isolation kit (Macherey-Nagel) according to manufacturer’s protocol ...
-
bioRxiv - Biophysics 2024Quote: ... NucleoSpin® plasmid isolation kit and NucleoSpin® gel and PCR clean-up kit were purchased from Macherey-Nagel (Germany). PierceTM protease inhibitor tablets EDTA free and Pierce™ high capacity Ni-IMAC resin were purchased from Thermo Scientific (international) ...
-
bioRxiv - Systems Biology 2023Quote: ... cDNA was generated by isolating RNA from 5×106 HEK293T cells with the Nucleospin RNA isolation Mini kit (Macherey-Nagel)according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... RNA from tissues or cells was isolated using an RNA isolation kit (NucleoSpin RNA, Macherey-Nagel GmbH & co.KG, Düren, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... We then stored the pellet at -20 °C for RNA isolation using the NucleoSpin RNA kit (MACHEREY-NAGEL, Düren, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Total RNAs were extracted from about 6 ×106 HepaRG cells using a Nucleospin RNA isolation Kit (Macherey-Nagel, Hoert, France) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: RNA from human prostate cancer cell lines was extracted using NucleoSpin® RNA isolation kit from Macherey-Nagel (Ref: 740955.240C), following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... Tissue homogenates were resuspended in RNA lysis buffer and RNA was isolated using the NucleoSpin RNA Plus RNA isolation kit (Macherey-Nagel). For some experiments ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA from each sample was isolated using the NucleoSpin® RNA isolation kit (Macherey-Nagel, Hoerdt Cedex, France) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... the genomic DNA from the edited cells was isolated after the transduction for eight days using a DNA isolation kit (NucleoSpinBlood - Macherey-Nagel). The target region for base editing was amplified using the respective primers (Sup Tables 2 and 3) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... isolation of vRNA from 150 µL cell-free supernatants was carried out with the NucleoSpin RNA virus kit (Macherey-Nagel, 740956) as described in the manufacturers’ instructions ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted from 30 mg of lice (15 lice) using the Total RNA isolation Nucleospin RNA kit (Macherey-Nagel) according to the manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2021Quote: ... total RNA was extracted from mESCs and MEFs (3 biological replicates of WT and Atxn7l3−/− for each cell types) using the NucleoSpin RNA isolation kit (Macherey-Nagel), according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel). The selection markers for fungal transformation were cloned into digested plasmids by using the Rapid DNA Ligation Kit (Thermo) ...
-
bioRxiv - Microbiology 2022Quote: ... The 0.22 µm membrane was used for bacterial DNA isolation using NucleoMag DNA/RNA Water Kit (MACHEREY-NAGEL Inc., Bethlehem, PA) following manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... homogenized and frozen on dry ice in 350 μl of Buffer RA1 containing BME (Macherey-Nagel Nucleospin RNA isolation kit 740955), and stored at –80°C ...
-
bioRxiv - Bioengineering 2023Quote: ... supernatant was removed and cell pellets were used for plasmid isolation by using NucleoBond□ Xtra Midi Plasmid DNA Purification Kit (Macherey-Nagel) according to the protocol supplied by the kit.
-
bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from different tissues (leaf, xylem, phloem, and root) using a commercial RNA isolation kit (Macherey-Nagel, Germany). According to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: RNA was extracted from a 145 μL volume of the clarified supernatant of each homogenized sample or virus stock using NucleoMag RNA isolation (Macherey-Nagel) either manually or in a EpiMotion 5075vt liquid handler ...
-
bioRxiv - Cell Biology 2024Quote: Total RNA from MCF7 cells was obtained with a Macherey-Nagel™ Total RNA Isolation kit (Macherey-Nagel, Germany, Cat # 740955.50) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: RNA was isolated from A375-C and A375-Cx43 untreated cell lines and under BRAF/MEKi (dabrafenib and trametinib) treatment (n=4, each condition) using the Kit RNA isolation: NucleoSpin RNA (Macherey-Nagel). Quality and integrity of each RNA sample was checked using a Bioanalyzer 2100 instrument (Agilent ...
-
bioRxiv - Cell Biology 2024Quote: ... The cloned gRNA + pSpCas9(BB)-2A-Puro V2.0 constructs were then isolated from the bacterial culture using a plasmid isolation kit (Macherey-Nagel, #740588.50). Following isolation ...
-
bioRxiv - Genetics 2022Quote: RNA extraction was performed from frozen tissue in culture plates (P6 well plate, Falcon®) by using the NucleoSpin RNA kit (Macherey-Nagel). RNA concentration was measured with NanoDrop1000 spectrophotometer (Thermo Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... 5000-10 000 ThyMCs were sorted into lysis buffer and the RNA was extracted using the NucleoSpin® RNA XS RNA isolation kit (Macherey-Nagel). Total RNA was reverse transcribed into cDNA using the SuperScript IV First-Strand Synthesis System (Thermo Fisher) ...
-
bioRxiv - Immunology 2023Quote: Total RNA was extracted from microglia after CD11b microbead isolation from P7 neonates born to and nursed by WT and Slc2a5−/− dams using the NucleoSpin RNA kit (Macherey-Nagel, 740955.250). The QuantiTect Reverse Transcription Kit (Qiagen ...
-
bioRxiv - Microbiology 2023Quote: ... exonuclease V was used to digest the linear DNA in the sample prior to isolation of the residual putative cirDNA using a DNA clean-up kit (NucleoSpin gel and PCR clean-up kit, Macherey-Nagel, Germany). The final cirDNA preparation was dissolved in TE buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were harvested by scraping in RA1 lysis buffer and total RNA was isolated using the NucleoSpin RNA Mini Kit for RNA Isolation (Macherey-Nagel, 740955) according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... about 1×103 – 1×104 cells were subjected to total RNA isolation using NucleoSpin RNA Plus XS (MACHEREY-NAGEL GmbH & Co. KG). The first-strand cDNA was synthesized by SuperScript III (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: RNA from cell culture supernatant after CPZ/PCZ treatment (mentioned above) was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG). Real-time quantitative RT-PCR was performed in Roche LightCycler 480 using commercial kits ...
-
bioRxiv - Physiology 2021Quote: ... mRNA specimens were isolated from the cells grown on collagen-gel in 24-well dishes using a NucleoSpin RNAplus RNA isolation kit (Macherey-Nagel, Dueren, Germany) and converted to cDNA with SuperScript IV VILO Master Mix (Invitrogen ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 2 ml of mutant culture from propagation round 3 were used for gDNA isolation using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). Whole genome sequencing was performed via the INVIEW Resequencing service for bacterial genomes up to 10 Mb with an Illumina standard genomic library provided by Eurofins Genomics (Ebersberg ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from fresh mycelium and conidia suspension after single-ascospore isolation and growth for five days on MEA media using the Nucleospin soil kit (Macherey-Nagel, Düren, Germany). For checking that isolated spores were recombinant offspring and not asexual conidia ...
-
bioRxiv - Genetics 2024Quote: Total RNA from twenty heads of 3 day-old Drosophila adults for each genetic combination was purified using the RNA isolation kit of Macherey-Nagel (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions.1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2022Quote: ... then genomic DNA was isolated using the Sodium Dodecyl Sulfate (SDS) procedure of the NucleoSpin Plant II DNA isolation kit (Macherey-Nagel, Dueren, Germany). Preparation of DNA libraries for bisulfite sequencing was performed as described in (Nunn et al. ...
-
bioRxiv - Bioengineering 2022Quote: Cultured cells were lysed in Macherey-Nagel RA1 buffer containing 1% β-mercaptoethanol and total RNAs were isolated using NucleoSpin RNA isolation kit (Macherey-Nagel 740955). Reverse transcription and PCR amplification were performed as described before21 ...
-
bioRxiv - Biochemistry 2020Quote: ... Individual quenched timepoints were spotted PEI-TLC plates (Macherey-Nagel) and nucleotides were resolved in 1.5 KH2PO4 pH 3.5 buffer ...
-
bioRxiv - Microbiology 2023Quote: ... silica gel G60 plates (10 x 20 cm, Macherey-Nagel) were used to spot samples and lipids were separated with CHCl3/CH3OH (90:10 ...
-
bioRxiv - Plant Biology 2024Quote: ... Twenty seedlings were pooled per replicate and total RNA was extracted from homogenised plant tissue using a phenol-based RNA isolation method (NucleoZol, Macherey-Nagel, Catalogue No.: 740404). PolyA+ libraries were prepared by and sequenced at BGI Genomics (Hong Kong ...
-
bioRxiv - Genomics 2024Quote: ... tissues were immediately frozen in liquid nitrogen and stored at -80°C prior to total RNA isolation with NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel Inc., PA, US). RNA libraries for RNA-Seq were prepared using the Illumina TruSeq Stranded Total RNA Ribo-Zero Plant kit (Illumina ...
-
bioRxiv - Microbiology 2021Quote: RNA from VTMs or swab-immersed TE buffer or cell culture supernatant was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG; 740956.250). The SARS-CoV2 RNA was quantified using (LabGun™ COVID-19 RT-PCR Kit ...
-
bioRxiv - Plant Biology 2020Quote: ... DNA was extracted using either a MO BIO PowerPlant DNA Isolation Kit (MO BIO Laboratories, Carlsbad, California, USA) or NUCLEOSPIN Plant II Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). Dry plant leaf material (typically ...
-
bioRxiv - Genetics 2021Quote: ... Vacuum the EconoSpin plate on the vacuum manifold (MACHEREY-NAGEL, Dueren, Germany) until no droplet drop down ...
-
bioRxiv - Plant Biology 2020Quote: PCR products were purified by NucleoFast 96 PCR plates (Macherey-Nagel, Germany) and were sequenced directly on both DNA strands on Applied Biosystems (Weiterstadt ...
-
bioRxiv - Microbiology 2021Quote: ... The amplicons were then purified using NucleoFast 96 PCR Plate (Macherey-Nagel) coupled to a vacuum pump ...