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Citations for Macherey-Nagel GmbH :
101 - 150 of 185 citations for 7 8 9 10 TETRAHYDRO 6H AZEPINO 2 1 B QUINAZOLIN 12 YLIDENEAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 4°C for 45 min) and the supernatant was incubated for 25 min at 4°C with 2 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Neuroscience 2020Quote: ... The total proteins were precipitated and prepared for Polyacrylamide Gel Electrophoresis using protein precipitator and resuspension buffer PSB/TCEP (Protein solving buffer and (tris(2-carboxyethyl)phosphine) TCEP reducing agent) from the NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France). The elution fraction containing RNA was further used for PCR analysis (see below) ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µL of extracts were injected into a Nucleoshell RP 18 column (2.7 µm x 150 mm x 2 mm, Macherey-Nagel GmbH, Düren, Germany). A solvent system composed of A ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a 10% SDS-polyacrylamide gels and transferred onto a 0.45 µm pore size nitrocellulose membrane (Macherey-Nagel GMBH: 741280) by wet blotting ...
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bioRxiv - Cell Biology 2023Quote: ... and 1:100 RNase A (MACHEREY-NAGEL, 740505) was added in each sample for 15 min at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... equipped with a photodiode array detector and a semi-preparative RP-Nucleodur C18 Htec column (5 µm, 250 × 10 mm, Macherey-Nagel, Düren, Germany) using a water-acetonitrile gradient ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... containing 10 nM internal standard, and 100 µL were transferred into mass spectrometry vials (Wicom, Heppenheim, Germany) with inserts (Macherey-Nagel, Düren, Germany) for carprofen quantification ...
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bioRxiv - Microbiology 2020Quote: ... The lysed culture was centrifuged at 46,000 x g for 1 hour and the supernatant was loaded onto a 1-ml Protino® Ni-NTA column (Macherey-Nagel). The protein was eluted with buffer A + 300 mM imidazole and protein-containing fractions were pooled and dialyzed overnight in buffer A with TEV protease (1/10 w/w ratio ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 100 μL of 1:1 diluted apical wash using the NucleoMag VET (Macherey-Nagel AG, Oensingen, Switzerland), according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... about 1×103 – 1×104 cells were subjected to total RNA isolation using NucleoSpin RNA Plus XS (MACHEREY-NAGEL GmbH & Co. KG). The first-strand cDNA was synthesized by SuperScript III (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Both peptide segments were purified by C18 reverse phase (RP)-HPLC on a Nucleosil C18 column (300 Å, 250 × 10 mm) (Macherey-Nagel, Düren, Germany), maintained at 25°C ...
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bioRxiv - Genomics 2021Quote: ... Menaquinones were then removed from the plate and analyzed using HPLC fitted with a reverse phase column (Macherey-Nagel, 2 mm x 125 mm, 3 µm, RP18) with methanol ...
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The complete mitochondrial genome of Spurilla braziliana MacFarland 1909 (Nudibranchia, Aeolidiidae)bioRxiv - Genomics 2023Quote: ... Japan (Figure 1) and preserved in NucleoProtect RNA (MACHEREY-NAGEL) for 5.5 months at -80 °C before DNA extraction ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was loaded onto 1 ml Ni-NTA agarose beads (Macherey-Nagel), pre-equilibrated with 10 ml wash buffer and rotated end-over-end for 1h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 mM NaCl and 1 mg/ml proteinase K (Macherey-Nagel, 740506) and 0.05 mg/ml RNase A (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... The frozen pellet was resuspended in 1 ml of RA1 buffer (Macherey-Nagel) supplemented 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Genomics 2023Quote: ... The ground tissues were lysed with 1 ml CF lysis buffer (MACHEREY-NAGEL) supplemented with 20 μl RNase and incubated for 1 h at 65°C under agitation ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Solid-phase extraction cartridges (Chromabond HR-X, 30mg, 1 ml, Macherey-Nagel, Dueren, Germany) were conditioned with 1 ml MeOH followed by 1 ml distilled HPLC-water ...
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bioRxiv - Plant Biology 2020Quote: ... The pellet was resuspended on ice in 1 ml NucleoZol (Macherey-Nagel, Düren, Germany) and incubated for 10 minutes at 65°C with shaking at 250 rpm ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from 1 × 106 cells using NucleoSpin DNA RapidLyse (MACHEREY-NAGEL). qPCR was performed with primers targeting the ND2 locus in the mitochondrial genome (5′-TGTTGGTTATACCCTTCCCGTACTA-3′ and 5′- CCTGCAAAGATGGTAGAGTAGATGA-3′ ...
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bioRxiv - Microbiology 2023Quote: ... 29-18-1 using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from 1×106 cells using a standard protocol (Macherey-Nagel Nucleospin). Sequencing library preparation was performed using the KAPA HyperPlus Library Preparation kit and associated protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from 1 mL overnight culture using NucleoSpin Plasmid Easypure columns (Macherey-Nagel) from the Plasmid Easypure Kit (Nucleospin).
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with preequilibrated Protino Ni-NTA beads (1 ml resin, Macherey-Nagel) at 4°C for 1h ...
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bioRxiv - Microbiology 2023Quote: ... PA14 and PAK from ∼1 × 108 CFU using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel), as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 1 ml supernatant containing Upa2 variant was added directly to the washed Ni-NTA (Macherey-Nagel) bead ...
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bioRxiv - Evolutionary Biology 2022Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Gibson assembly was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... Pre_S or FECD corneal endothelial tissues (Supplemental Table 1) by NucleoSpin RNA XS kit (Macherey-Nagel). cDNAs were prepared by reverse transcription ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM PMSF) by sonication followed by loading on to Ni-IDA column (Macherey-Nagel, Germany). Endonuclease G was eluted in gradient imidazole concentration (100-500 mM ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was first extracted from 1 x 105 cells using NucleoSpin Tissue (MACHEREY-NAGEL #740952) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was then loaded on a Protino® GST/4B column 1 ml (Macherey-Nagel) for pull-down experiments ...
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bioRxiv - Systems Biology 2019Quote: ... RNA was recovered from 1 x 106 cells using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The RNA was sent to Eurofins for RNA-seq analysis ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µl from each sample was spotted onto a POLYGRAM CEL 300 PEI-TLC plate (Macherey-Nagel), and developed with 0.5 M LiCl (Sigma) ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The PCR product (325 bp) was purified from a 1% agarose gel via NucleoSpin columns (Macherey-Nagel). NGS was performed on an Illumina Miniseq using a MiniSeq Mid Output Kit (300-cycles ...
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bioRxiv - Genomics 2023Quote: ... was purified from a 1% agarose gel using a Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 4°C) and the supernatant was passed through a 1 ml Ni-NTA column (Protino, Macherey-Nagel) utilizing liquid chromatography (ÄKTApurifier) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean‑up (Macherey-Nagel, Düren, Germany). Following digestion with restriction enzymes ...
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bioRxiv - Evolutionary Biology 2019Quote: The pH of individual reaction mixtures was determined via TRITEST L pH 1–11 pH papers (Macherey-Nagel) directly after the reaction ...
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bioRxiv - Biochemistry 2022Quote: ... Table 1) followed by column purification using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). After sequencing the purified PCR product ...
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bioRxiv - Genomics 2024Quote: ... cells were harvested at OD=1 for plasmid extraction using the NucleoBond Xtra Midi kit (Macherey-Nagel; 740412.50). The composition of the final library was verified by deep sequencing.
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bioRxiv - Microbiology 2020Quote: ... the soluble fraction was directly loaded onto a 1-ml nickel column (Protino Ni-nitrotriacetic acid [NTA]; Macherey-Nagel). The column was washed with Wash Buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... Products were then run on a 1% agarose gel and the gel was extracted using NucleoSpin (Macherey-Nagel, 740588.50) clean up kit according to manufacturer specifications.
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bioRxiv - Molecular Biology 2022Quote: ... Resulting PCR products were purified from 1% agarose gels with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel) to prevent the production of side products during in vitro transcription ...
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bioRxiv - Molecular Biology 2024Quote: 1 µg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 µl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441) ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA in the supernatant was precipitated with isopropanol and suspended in 250 μL of STE (10 mM Tris-HCl [pH 8.0], 1 mM EDTA [pH 8.0], 100 mM NaCl containing 80 μg/mL RNaseA [Macherey-Nagel]). RNA was digested by incubation for 1 hr at 37°C ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from 1 ml of crude homogenate containing 6 μl of proteinase K (22 mg/ml, Macherey-Nagel), incubated overnight at 55°C.
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bioRxiv - Plant Biology 2021Quote: ... 1 μL of sample was injected onto an RP18 column (4 × 50 mm, 2.7 μm particle size, Nucleoshell, Macherey-Nagel) with a flow rate of 0.25 mL min−1 ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA constructs for mammalian tissue culture (Supplementary Table 1) were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from around 5×105 to 1×106 cells using the NucleoSpin RNA Plus (MACHEREY-NAGEL, 740984.50) or Quick-RNA Miniprep Kit (Zymo Research ...