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Citations for Macherey-Nagel GmbH :
51 - 86 of 86 citations for Zika Virus Lysate DakArD 41662 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: HIV RNA was extracted from plasma samples collected from either HIV-1 infected or uninfected humanized hNSG mice transplanted with CD4+ T cells from PWH in the absence or presence of immunotherapy using NucleospinTM RNA Virus kit (Cultek, Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was loaded onto a 5 mL Ni-NTA column (Macherey-Nagel). After washing with a buffer consisting of 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The clarified lysate was subjected to affinity chromatography on Ni2+ agarose resin (Macherey-Nagel) and washed extensively with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of the permeabilized sample was then processed using the Machery-Nagel NucleoSpin Virus kit (Macherey-Nagel, Catalog # 740983.50 and 740983.250).
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bioRxiv - Microbiology 2020Quote: ... Clonal virus populations were characterized by isolating viral RNA from 150 µl aliquots of the cell culture supernatant with the NucleoSpin® RNA Virus kit (MACHEREY-NAGEL) followed by conventional RT-PCR and bidirectional Sanger sequence analysis.
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bioRxiv - Zoology 2024Quote: ... RNA from 150 µl of excreta solution was extracted by adding 600 µl of RAV1 lysis buffer and using NucleoSpin virus RNA kit (Macherey-Nagel, France).
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bioRxiv - Immunology 2022Quote: ... coli strain in presence of 100 μg/ml ampicillin and prepared by Midi DNA-prep kit (Macherey-Nagel, 740410) for the transient transfection ...
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bioRxiv - Microbiology 2020Quote: ... difficile strains was estimated by quantitative PCR on genomic DNA extracted using the NucleoSpin Microbial DNA kit (Macherey-Nagel). The total chromosome copy number was quantified based on the reference gene dnaF (CD1305 ...
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bioRxiv - Genetics 2019Quote: ... Total RNA was extracted from the lysate using NucleoSpin RNA Set for NucleoZOL (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Total RNAs were extracted from cell-lysates using the NucleoSpin RNA II Kit (Macherey-Nagel) following the manufacturer’s protocol and were eluted in nuclease-free water ...
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bioRxiv - Microbiology 2021Quote: Total RNAs were extracted from cell lysates using the NucleoSpin RNA II Kit (Macherey-Nagel) following the manufacturer’s protocol and were eluted in nuclease-free water ...
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bioRxiv - Biophysics 2022Quote: ... and the protein was affinity- purified from lysate using Ni-NTA affinity resin (Macherey-Nagel) and elution with imidazole ...
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bioRxiv - Microbiology 2024Quote: Total RNA from cell lysates was isolated with a NucleoSpin RNA purification kit (Macherey-Nagel). Purified whole cell RNA was converted to cDNA using the high-capacity cDNA reverse transcription kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: Total RNAs from cell lysates were extracted using the NucleoSpin RNA II kit (Macherey-Nagel) following the manufacturer’s protocol and were eluted in nuclease-free water ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA form cell lysates was obtained using a NucleoSpin® RNA kit (Macherey-Nagel), according to the manufacturer’s instructions and with on-column DNAse treatment ...
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bioRxiv - Developmental Biology 2024Quote: ... the cleared lysate was loaded onto a 5 mL Protino GST/4B column (Macherey-Nagel) and washed with 10 column volumes of lysis buffer ...
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bioRxiv - Epidemiology 2020Quote: ... 20 µL of Proteinase K were added to 180 µL of crushed tick sample and DNA was extracted using the NucleoSpin® 96 virus Core kit (Macherey-Nagel, Germany) and the automatic platform Biomek4000 (Beckman Coulter) ...
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bioRxiv - Microbiology 2019Quote: ... difficile strains was done using standard methods.33 Routine DNA extractions were performed using the Nucleospin Plasmid Easypure (Macherey-Nagel) and DNeasy Blood and Tissue (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... total RNA was extracted from all cell lysates using the NucleoSpin RNA II kit (Macherey-Nagel).
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bioRxiv - Biochemistry 2022Quote: ... The proteins were purified from the cleared lysate with Ni-NTA agarose (Macherey-Nagel, Düren, Germany) following concentration and dialysis against 0.1 M potassium phosphate buffer pH 7.
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bioRxiv - Immunology 2023Quote: Total RNA from cell lysates was extracted using the NucleoSpin RNA II kit (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from the lysates using the NucleoMag Vet kit (Macherey-Nagel, cat.no. 744200) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation and ZAP-S was captured using Ni-NTA resin (Macherey-Nagel). After elution with 500 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... and viral RNA from cell lysate was extracted using the Nucleospin RNA plus mini kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA from the LAB strains was extracted using a NucleoBond AXG column and Buffer Set III (U0544A & U0603A, MACHEREY-NAGEL, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... consecutive copies of 197 bp with modified 601 sequence were transformed into DH5α Escherichia coli strain and purified from four liters of culture by using plasmid DNA purification kit Nucleobond® PC 10000 (Macherey-Nagel). Three mg of the plasmids were digested with EcoRI HF (0.25 U/µg DNA ...
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bioRxiv - Microbiology 2022Quote: The genomic DNA of Rhodococcus strain IGTS8 was isolated using the NucleoSpin Tissue DNA Extraction kit (Macherey-Nagel, Lab Supplies Scientific SA, Hellas) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from cell-free lysate by metal ion affinity chromatography with Protino Ni-NTA Agarose (Macherey-Nagel) as matrix followed by ultrafiltration (Amicon Ultra-15 centrifugal filter units ...
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bioRxiv - Cell Biology 2019Quote: ... lysates prepared from infected and stimulated cells were collected and RNA extracted using a NucleoSpin RNA kit (Macherey-Nagel, 740955). The yield and purity of the extracted RNA was assessed using a spectrophotometer (DS-11 FX ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were centrifuged at 21000 g for 10 min and supernatants were transferred to silica membrane spin columns (Macherey-Nagel) and centrifuged at 11000 g for 1 min ...
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bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... His6-BsrA was purified from the cell lysate by chromatography on Ni-agarose columns (Protino Ni-TED 1000, Macherey-Nagel) with 300 mM imidazole in phosphate buffer used for elution ...
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bioRxiv - Biochemistry 2024Quote: ... Clarified lysates were filtered (0.45 µm syringe tip filters) and applied to pre-equilibrated Protino Ni-NTA Agarose (Macherey-Nagel) beats in a gravety column ...
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bioRxiv - Developmental Biology 2022Quote: ... All plasmids were cloned into the DH5a strain (Enzynomics) and purified with a NucleoBond Xtra Midi Plus kit (MACHEREY-NAGEL GmbH & Co. KG, Dueren, Germany) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... The lysates were then purified to genomic DNA and RNA using NucleoSpin Tissue Kits and NucleoSpin RNA Plus kits (MACHEREY-NAGEL), respectively ...
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bioRxiv - Biochemistry 2023Quote: ... After cell lysis using a French press the cell debris was removed by centrifugation at 17000 g for 1 h at 4 °C and the cleared lysate was incubated with Protino IDA resin (Macherey-Nagel) for 20 min at 4° C ...