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Citations for Macherey-Nagel GmbH :
51 - 100 of 2192 citations for Mouse Fragile X Mental Retardation 1 Neighbor Protein FMR1NB ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 1 × 106 cells with NucleoSpin Tissue kit (Macherey-Nagel), and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... The total proteins were precipitated and prepared for Polyacrylamide Gel Electrophoresis using protein precipitator and resuspension buffer PSB/TCEP (Protein solving buffer and (tris(2-carboxyethyl)phosphine) TCEP reducing agent) from the NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France). The elution fraction containing RNA was further used for PCR analysis (see below) ...
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bioRxiv - Cancer Biology 2019Quote: ... genomic DNA was isolated from the peripheral blood and tail tissues of each chimeric mouse 30 days after BM transfer using a NucleoSpin tissue kit (Macherey-Nagel, Duren, Germany). Then ...
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bioRxiv - Microbiology 2023Quote: ... 29-18-1 using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was isolated from peripheral blood and tail tissues of each chimeric mouse 6 weeks after BM transplantation with NucleoSpin® Tissue Kit (MACHEREY-NAGEL, Duren, Germany). Then ...
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bioRxiv - Microbiology 2023Quote: ... PA14 and PAK from ∼1 × 108 CFU using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel), as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... an EC 100/2 Nucleoshell Phenyl-Hexyl column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Plant Biology 2021Quote: ... a Nucleoshell RP 18 plus reversed-phase column (2 x 100 mm, 2.7 μm; Macherey-Nagel) was used for chromatographic separation ...
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bioRxiv - Biochemistry 2019Quote: ... Supernatants were depleted of undecanal and undecanal-modified peptides using HR-X (M) cartridges (Macherey-Nagel). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... SSC was separated on a 250 x 3 mm 5 µm Nucleodur HTec C18 (Macherey-Nagel) column ...
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bioRxiv - Molecular Biology 2020Quote: ... Pre_S or FECD corneal endothelial tissues (Supplemental Table 1) by NucleoSpin RNA XS kit (Macherey-Nagel). cDNAs were prepared by reverse transcription ...
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bioRxiv - Microbiology 2019Quote: ... Total nucleic acids were extracted from mouse samples submitted to IDEXX BioAnalytics using a commercially available platform (NucleoMag® VET Kit; Macherey-Nagel GmbH & Co. KG, Düren, Germany).
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bioRxiv - Genomics 2023Quote: ... was purified from a 1% agarose gel using a Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved protein was passed through Ni-TED (Macherey-Nagel) to remove uncleaved protein ...
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bioRxiv - Biochemistry 2023Quote: All proteins were purified using Ni-IDA (Macherey-Nagel) following the instructions provided by the manufacturer ...
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bioRxiv - Plant Biology 2021Quote: ... Separation of metabolites was achieved on a Nucleoshell RP18 column (100 x 4.6 mm, 2.7 µm; Macherey-Nagel, Germany). The column was equipped with a precolumn (Macherey–Nagel ...
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bioRxiv - Molecular Biology 2022Quote: ... Resulting PCR products were purified from 1% agarose gels with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel) to prevent the production of side products during in vitro transcription ...
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bioRxiv - Molecular Biology 2024Quote: 1 µg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 µl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441) ...
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bioRxiv - Molecular Biology 2020Quote: ... following which it was applied onto a pre-equilibrated (EQ buffer containing 0.15% Triton X-100) Nucleobond AX-100 column (Macherey-Nagel). The column-bound RNA was washed twice with wash buffer WB (10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA constructs for mammalian tissue culture (Supplementary Table 1) were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Biophysics 2022Quote: ... The product was then purified from 1% agarose gel and extracted using the NucleoSpin clean up kit (Macherey-Nagel, 740588.50).
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bioRxiv - Microbiology 2023Quote: ... phenol:chloroform:isoamylalcohol (25:24:1; Panreac AppliChem) method or 2) the guidelines of the NucleoSpin Plant II Mini kit (Macherey-Nagel). The integrity of the samples was checked by agarose electrophoresis (0.8 %).
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bioRxiv - Plant Biology 2022Quote: ... Proteins were batch-purified using Ni-NTA agarose resin (Macherey-Nagel) and eluted using the abovementioned lysis buffer with increased imidazole concentration (250 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Hydrolyzed samples were then purified and extracted using solid phase extraction cartridges (Chromabond HR-X, 30mg, 1ml, Macherey-Nagel, Dueren, Germany) placed onto a 12-port vacuum manifold ...
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bioRxiv - Plant Biology 2022Quote: ... The eluted desulfoglucosinolates were separated on a reversed phase C-18 column (Nucleodur Sphinx RP, 250 x 4.6 mm, 5µm, Macherey-Nagel, Düren, Germany) with a water (A)-acetonitrile (B ...
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bioRxiv - Biochemistry 2023Quote: ... The BoNT-X/NTNH complex was purified by gravity-flow affinity chromatography on Ni-NTA agarose resin (Macherey-Nagel, Düren, Germany) in lysis buffer ...
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bioRxiv - Microbiology 2024Quote: ... into prefilled tube strips (8 x 12) containing 750 µl DNA/RNA ShieldTM (ZymoBIOMICS) and 40-400 µm glass beads (Macherey-Nagel) and arranged on a 96-well rack ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 0.25 g (3.6 g TSS L−1) activated sludge was processed to extract intracellular DNA using a NucleoSpin® Soil kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA from each sample was isolated using the NucleoSpin® RNA isolation kit (Macherey-Nagel, Hoerdt Cedex, France) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Clones whose culture had proven successful (IgG concentration ≥ 1 μg/mL at day 21-25) were selected and extracted using the NucleoSpin96 RNA extraction kit (Macherey-Nagel) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Both PCR products were excised from a 1% agarose gel and extracted using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) and used as templates for In-Fusion HD cloning (Takara) ...
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bioRxiv - Genomics 2021Quote: ... Resulting amplicons were loaded on a 1% agarose gel and purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Libraries were sequenced paired-end 75bp on the NextSeq 500 platform yielding approximately 4*106 fragments per sample (Supplementary Table 2) ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from three independent experiments of the mock- and KSHV-infected lymphatic ECFCs isolated as an adherent subpopulation from donor 1 using the NucleoSpin RNA extraction kit (Macherey-Nagel), after which purity and concentration was determined with NanoDrop spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Barcoded amplicons were extracted from agarose gel (1.5%, 1 h, 100 V) by using NucleoSpin® Gel and PCR Cleanup XS kit (Macherey-Nagel) and the amplicon size and DNA concentration were checked with High Sensitivity D1000 ScreenTape assay in 2200 TapeStation System (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and verified by Sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and sent for Sanger sequencing.
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bioRxiv - Physiology 2023Quote: ... Products were run on 1% agarose gels and then gel-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Clones whose culture had proven successful (IgG concentration ≥ 1 μg/mL at day 21-25) were selected and extracted using the NucleoSpin96 RNA extraction kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from Kasumi-1 cells after 2 days after shRUNX1::ETO knockdown was induced with doxycycline using the Nucleospin RNA kit (Macherey-Nagel). cDNA was synthesised using Superscript II (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products were visualized on a 1% TAE agarose gel and purified using the Nucleospin PCR Clean-up Kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from immature inflorescences (stages 1-12) or aerial parts of 16- day-old seedlings using NucleoSpin RNA Plus kit (Macherey-Nagel) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: DNA was extracted from blood samples of patients II-8 and IV-1 using the Nucleospin extraction kit (ref 740952-Macherey-Nagel). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified using Protino NTA columns (Macherey-Nagel, Freemansburg, PA, USA) using linear imidazole gradients (30-250 mM) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified at 4 °C on Ni-NTA-Sepharose (Macherey-Nagel) packed in a gravity flow column ...
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bioRxiv - Immunology 2022Quote: ... The protein was purified on a Ni-NTA resin column (Macherey-Nagel) and the eluted fractions were concentrated and dialyzed against PBS using the same Amicon system as described above ...
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bioRxiv - Cell Biology 2020Quote: ... or Protino Ni-TED resin (for SUMO-tagged proteins, Macherey-Nagel, #745200.5) for 1 h at 4 °C with rotation and then transferred to gravity columns (Talon® 2 ml Disposable Gravity Column ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were then transferred onto Porablot NCP nitrocellulose membrane (Macherey-Nagel) and blocked for 1 hour at RT in 3% BSA (Applichem ...
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bioRxiv - Evolutionary Biology 2020Quote: Fifteen microliters of extract were injected into an Agilent 1100 series HPLC and compounds were separated on an EC 150/4.6 NUCLEODUR® C18 Gravity column (3 μm, 150 mm x 4.6 mm, Macherey-Nagel, Düren, Germany). Cardenolides and bufadienolides were eluted at a constant flow of 0.7 ml/min at 30°C with an acetonitrile–H2O gradient as follows ...